期刊
ATHEROSCLEROSIS
卷 289, 期 -, 页码 36-43出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.atherosclerosis.2019.08.002
关键词
Biomarkers; microRNAs; Ischaemic stroke; Thrombolysis
资金
- Biomedicine Key Programme of the Shanghai Municipal Science and Technology Commission [16411953100]
- National Natural Science Foundation of China [81271302]
- Shanghai Municipal Science and Technology Commission [18dz2313603, 14JC1404300]
- Prevention and Control of Chronic Diseases Project of the Shanghai Hospital Development Center [SHDC12015310]
- project of Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support [20161422]
- Clinical Research Project of Shanghai Jiao Tong University School of Medicine [DLY201614]
Background and aims: Circulating microRNAs (miRNAs) have recently emerged as promising biomarkers for acute ischaemic stroke (AIS). However, the expression profiles of miRNAs in AIS patients receiving intravenous thrombolysis, and their associations with outcome have not been investigated. Methods: In a prospective cohort study, a total of 84 AIS patients, who received intravenous thrombolysis (21.4% received combined reperfusion therapy) and completed 3 month follow-up visits, were included. Favourable and unfavourable outcomes were defined as modified Rankin Scale (mRS) scores of 0-1 and 2-6, respectively. Plasma samples were collected at 24 h after thrombolysis. We used RNA sequencing to study miRNA profiles in 5 patients with unfavourable outcomes and 5 matched patients with favourable outcomes. Differentially expressed miRNAs were further validated in all cohorts using quantitative real-time polymerase chain reaction assays. Results: After identification and validation, we found that miR-124-3p, miR-125b-5p and miR-192-5p levels were higher in patients with unfavourable outcomes than in patients with favourable outcomes. Logistic regressions and receiver-operating characteristic curve analyses demonstrated that these altered miRNAs may function as predictive biomarkers for outcome in AIS patients receiving thrombolysis, whether combined with endovascular thrombectomy or not. In addition, miR-124-3p and miR-125b-5p were closely associated with stroke severity. Conclusions: A set of circulating microRNAs (miR-124-3p, miR-125b-5p and miR-192-5p) are associated with unfavourable 3 month outcomes and might have clinical utility in AIS patients receiving thrombolysis.
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