Article
Microbiology
Younjung Kim, Maura Carrai, Marcus H. Y. Leung, Jaime Chin, Jun Li, Patrick K. H. Lee, Julia A. Beatty, Dirk U. Pfeiffer, Vanessa R. Barrs
Summary: The study found that the feline bladder microbiome is typically sparse, with various phyla, families, and genera identified. Different urotypes were associated with disease status, with the Escherichia-Shigella-predominant urotype being common in CKD cases. The microbial composition of CKD cases was more similar to that of E. coli PUC cases than to controls, indicating potential implications for the development of clinical infections.
Article
Immunology
Mohamed Kettani Halabi, Fatima Azzahra Lahlou, Idrissa Diawara, Younes El Adouzi, Rabiaa Marnaoui, Rachid Benmessaoud, Imane Smyej
Summary: ESBL-EC infections pose challenges due to limited treatment options, increased hospital costs, reduced microbiological and clinical responses, and a threat to patient's life. Majority of ESBL-EC isolates were from female adult patients over 50, resistant to third-generation cephalosporins and quinolones, but sensitive to carbapenems and fosfomycin.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Eric Farfour, Laurent Dortet, Thomas Guillard, Nicolas Chatelain, Agathe Poisson, Assaf Mizrahi, Damien Fournier, Remy A. Bonnin, Nicolas Degand, Philippe Morand, Frederic Janvier, Vincent Fihman, Stephane Corvec, Lauranne Broutin, Cecile Le Brun, Nicolas Yin, Genevieve Hery-Arnaud, Antoine Grillon, Emmanuelle Bille, Helene Jean-Pierre, Marlene Amara, Francoise Jaureguy, Christophe Isnard, Vincent Cattoir, Tristan Diedrich, Emilie Flevin, Audrey Merens, Herve Jacquier, Marc Vasse
Summary: There are some trends in antibiotic resistance among Enterobacterales isolates recovered from urinary tract samples in France. Certain carbapenem-sparing drugs remain highly active.
Article
Biochemistry & Molecular Biology
Nabi Jomehzadeh, Morteza Saki, Khadijeh Ahmadi, Golshan Zandi
Summary: This study aimed to determine the prevalence of quinolone resistance and the presence of plasmid-mediated quinolone resistance (PMQR) genes among extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates. The results showed a high resistance rate to nalidixic acid and ciprofloxacin among ESBL-producing isolates. The most frequent PMQR gene was aac(6')-Ib-cr variant. The coexistence of aac(6')-Ib-cr variant and qnrB was the most widely distributed genotype among quinolone resistance isolates.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Infectious Diseases
Adriana Belas, Catia Marques, Juliana Menezes, Luis Telo da Gama, Patricia Cavaco-Silva, Constanca Pomba
Summary: This study aimed to compare ESBL/pAmpC-producing E. coli strains causing community-acquired UTI in companion animals and humans. The study found that companion animals and humans shared two multidrug-resistant high-risk clonal lineages: ST131 and ST648. The study also reported two newly discovered clonal lineages in humans with community-acquired UTI in Portugal.
Article
Health Care Sciences & Services
Adil Abalkhail, Ahmad S. AlYami, Saeed F. Alrashedi, Khalid M. Almushayqih, Thamer Alslamah, Yasir Ahmed Alsalamah, Ayman Elbehiry
Summary: This study aims to investigate the antibiotic resistance of ESBL-E. coli among male and female patients with urinary tract infections in Riyadh, Saudi Arabia. The research found that most ESBL-E. coli isolates were resistant to cephalosporins and penicillin, but sensitive to carbapenems, aminoglycosides, and nitrofurantoins.
Article
Infectious Diseases
Stephanie Larramendy, Aurelie Gaultier, Jean-Pascal Fournier, Jocelyne Caillon, Leila Moret, Francois Beaudeau
Summary: The prevalence of ESBL-producing E. coli in community-acquired UTI varies significantly worldwide, and in this study, various anthropogenic factors were found to be associated with the local ESBL-E. coli rate, providing useful information for improving risk management.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Leah J. Toombs-Ruane, Jonathan C. Marshall, Jackie Benschop, Dragana Drinkovi, Anne C. Midwinter, Patrick J. Biggs, Zoe Grange, Michael G. Baker, Jeroen Douwes, Mick G. Roberts, Nigel P. French, Sara A. Burgess
Summary: This study aimed to assess whether having a pet in the home is a risk factor for community-acquired urinary tract infections associated with extended-spectrum β-lactamase (ESBL)-or AmpC β-lactamase (ACBL)-producing Enterobacterales. Through telephone questionnaire and logistic regression analysis, it was found that pet ownership was not associated with ESBL-or ACBL-producing Enterobacterales-related urinary tract infections, while the main risk factors included recent antimicrobial treatment, travel to Asia in the previous year, and a doctor's visit in the last 6 months.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Immunology
Thijs ten Doesschate, Sander Kuiper, Cees van Nieuwkoop, Robert-Jan Hassing, Tom Ketels, Suzan P. van Mens, Wouter van den Bijllaardt, Akke K. van der Bij, Suzanne E. Geerlings, Ad Koster, Evert L. Koldewijn, Judith Branger, Andy I. M. Hoepelman, Cornelis H. van Werkhoven, Marc J. M. Bonten
Summary: Fosfomycin is noninferior to ciprofloxacin as oral step-down treatment for febrile urinary tract infections (fUTIs) caused by E. coli in women, but its use is associated with more gastrointestinal events.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Microbiology
Amanda Brumwell, Granger Sutton, Paul M. M. Lantos, Kate Hoffman, Felicia Ruffin, Lauren Brinkac, Thomas H. H. Clarke, Mark D. D. Adams, Vance G. G. Fowler Jr, Derrick E. E. Fouts, Joshua T. T. Thaden
Summary: This study aims to further understand the risk factors, clinical outcomes, and bacterial genetics associated with ST131 bloodstream infections. A cohort study was conducted in which 88 out of 227 patients with E. coli bloodstream infection were infected with ST131. There was no significant difference in in-hospital mortality between patients with ST131 and non-ST131 infections overall, but ST131 was associated with higher mortality in patients with urinary tract infections.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Microbiology
S. J. Ryan Arends, Deborah Butler, Nicole Scangarella-Oman, Mariana Castanheira, Rodrigo E. Mendes
Summary: The in vitro activities of gepotidacin against a large collection of Escherichia coli and Staphylococcus saprophyticus strains, including those resistant to other standard-of-care antibiotics, were determined. Gepotidacin exhibited potent activity, inhibiting the majority of isolates at low concentrations.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Medicine, General & Internal
Katarzyna Werbel, Dorota Jankowska, Anna Wasilewska, Katarzyna Taranta-Janusz
Summary: The study analyzed the increasing resistance of bacteria in children with UTI over the years, identified parameters related to antibiotic sensitivity using the CART method, and provided guidance for antibiotic therapy to inhibit increasing antibiotic resistance.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Microbiology
Hyeon Jin Choi, Seok Hoon Jeong, Kyeong Seob Shin, Young Ah Kim, Young Ree Kim, Hyun Soo Kim, Jong Hee Shin, Jeong Hwan Shin, Young Uh, Songmee Bae, Eun-Jeong Yoon, Jung Sik Yoo
Summary: This study assessed 636 cases of E. coli urinary tract infections in South Korea, identifying risk factors for bloodstream infections and characterizing the genetic diversity of E. coli isolates. The findings provide insights for treatment options and the prevalence of antimicrobial-resistant organisms.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Guadalupe Miranda-Novales, Karen Flores-Moreno, Yolanda Lopez-Vidal, Samuel Ponce de Leon-Rosales
Summary: This study evaluated the antimicrobial susceptibility patterns of Escherichia coli and Klebsiella pneumoniae, and found that multidrug-resistant bacteria represented 61% of all isolates.
Review
Nutrition & Dietetics
Reeta Ala-Jaakkola, Arja Laitila, Arthur C. Ouwehand, Liisa Lehtoranta
Summary: Urinary tract infections (UTIs) are a common bacterial disease worldwide, and antibiotic resistance is a major concern. D-mannose, a dietary supplement, shows promise as an alternative treatment for UTIs, reducing the risk and recurrence.
Article
Infectious Diseases
Kelly L. Wyres, Jane Hawkey, Marit A. K. Hetland, Aasmund Fostervold, Ryan R. Wick, Louise M. Judd, Mohammad Hamidian, Benjamin P. Howden, Iren H. Lohr, Kathryn E. Holt
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2019)
Article
Infectious Diseases
Olav Bjarte Natas, Anita Lovas Brekken, Eva Bernhoff, Marit Andrea Klokkhammer Hetland, Iren Hoyland Lohr, Paul Christoffer Lindemann
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2019)
Article
Microbiology
Fredrik Hakonsholm, Marit A. K. Hetland, Cecilie S. Svanevik, Arnfinn Sundsfjord, Bjorn Tore Lunestad, Nachiket P. Marathe
Article
Infectious Diseases
Oskar Ekelund, Marit Andrea Klokkhammer Hetland, Iren Hoyland Lohr, Thomas Schon, Sofia Somajo
Summary: A rapid and sensitive flow cytometry-based colistin resistance testing method for E. coli and K. pneumoniae was developed and shown to have higher efficiency and accuracy compared to the traditional BMD method.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Aasmund Fostervold, Marit A. K. Hetland, Ragna Bakksjo, Eva Bernhoff, Kathryn E. Holt, Orjan Samuelsen, Gunnar Skov Simonsen, Arnfinn Sundsfjord, Kelly L. Wyres, Iren Hoyland Lohr
Summary: The study used nationwide surveillance data to examine longitudinal changes in Klebsiella pneumoniae isolates in Norway, focusing on ESBL-producing strains. The results revealed a diverse ESBL population dominated by specific clonal groups carrying bla(CTX-M-15), indicating convergence of virulence and resistance determinants. Klebsiella variicola was found to be a frequent cause of infection but rarely carried ESBLs.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Public, Environmental & Occupational Health
Fredrik Hakonsholm, Marit A. K. Hetland, Cecilie S. Svanevik, Bjorn Tore Lunestad, Iren H. Lohr, Nachiket P. Marathe
Summary: This study investigates the genetic diversity, resistome, and pathogenic potential of Kp isolates from the Norwegian marine environment using whole-genome sequencing. The study provides the first comprehensive account of genetic diversity among Kp from the marine environment and reveals a high diversity of Kp in the Norwegian marine environment and seafood, including globally disseminated pathogenic sequence types carrying clinically relevant antibiotic resistance genes and virulence factors, as well as several heavy metal resistance genes.
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2022)
Article
Genetics & Heredity
Marit A. K. Hetland, Jane Hawkey, Eva Bernhoff, Ragna-Johanne Bakksjo, Hakon Kaspersen, Siren I. Rettedal, Arnfinn Sundsfjord, Kathryn E. Holt, Iren H. Lohr
Summary: Klebsiella pneumoniae ST17 is a globally disseminated clone that causes multidrug-resistant hospital infections. An outbreak of MDR ST17 occurred at a neonatal intensive care unit in Norway, with long-term colonization observed in the children even after discharge. The within-host evolution of ST17 during colonization showed stability with minimal genetic changes and persistent carriage of a specific plasmid. ST17 has diverse lineages with various AMR genes and virulence loci, but many strains do not acquire AMR. The non-human sources and human colonization may play a crucial role in severe infections.
MICROBIAL GENOMICS
(2023)
Article
Genetics & Heredity
Hakon Kaspersen, Fiona Valerie Franklin-Alming, Marit A. K. Hetland, Eva Bernhoff, Iren H. Lohr, Jatesada Jiwakanon, Anne Margrete Urdahl, Thongpan Leangapichart, Marianne Sunde
Summary: In this study, Klebsiella pneumoniae isolated from healthy fattening pigs in Norway were characterized. The results showed a high detection rate of the pathogen in pig caecal samples, and most of the isolates carried acquired virulence determinants, including the siderophore aerobactin. In contrast, only a few pig isolates from Thailand harbored aerobactin. The virulence genes were located on potentially transferable plasmids and were associated with a predicted composite transposon.
MICROBIAL GENOMICS
(2023)
Article
Microbiology
Fredrik Hakonsholm, Marit A. K. Hetland, Iren H. Loehr, Bjorn Tore Lunestad, Nachiket P. Marathe
Summary: This study aimed to determine the genetic background of antibiotic and heavy metal resistance genes in antibiotic-resistant K. pneumoniae isolated from marine bivalves. Complete genome sequencing was conducted on five antibiotic-resistant isolates, and plasmid transfer potential was examined through conjugation experiments. The results revealed the co-occurrence of antibiotic and heavy metal resistance genes on a conjugative IncFIB plasmid, highlighting the potential dissemination route for antimicrobial resistance through the marine environment and seafood.
Article
Gastroenterology & Hepatology
Niclas Raffelsberger, Marit Andrea Klokkhammer Hetland, Kristian Svendsen, Lars Smabrekke, Iren Hoyland Lohr, Lotte Leonore Eivindsdatter Andreassen, Sylvain Brisse, Kathryn E. Holt, Arnfinn Sundsfjord, Orjan Samuelsen, Kirsten Gravningen
Summary: The study found that older individuals, those who have traveled to Greece or Asia, those with Crohn's disease/ulcerative colitis, users of proton pump inhibitors, non-steroidal anti-inflammatory drugs, and antibiotics were more likely to carry antibiotic resistant Klebsiella pneumoniae. The diverse population structure of colonizing isolates reflects the bacterium's adaptive capacity and poses challenges for vaccine development and identifying reservoirs as a potential source for human colonization.