期刊
BIOCONJUGATE CHEMISTRY
卷 27, 期 4, 页码 1153-1164出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.bioconjchem.6b00102
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资金
- Michael J. and Sharon R. Bukstein Chair in Cancer Research
- Coulter Foundation
- Fundacao para a Ciencia e Tecnologia (FCT) [EXCL/QEQ-MED/0233/2012]
- FCT [UID/Multi/04349/2013, PEst-OE/CTM/LA0024/2013, SFRH/BD/47308/2008]
- COST Action [TD1004]
- Programa Nacional de Reequipamento Cientifico of FCT [REDE/1503/REM/2005 - ITN]
- RNEM - Rede Nacional de Espectrometria de Massa
- Fundação para a Ciência e a Tecnologia [SFRH/BD/47308/2008] Funding Source: FCT
To get a better insight on the transport mechanism of peptide-conjugated nanoparticles to tumors, we performed in vivo biological studies of bombesin (BBN) peptide functionalized gold nanoparticles (AuNPs) in human prostate tumor bearing mice. Initially, we sought to compare AuNPs with thiol derivatives of acyclic and macrocyclic chelators of DTPA and DOTA types. The DTPA derivatives were unable to provide a stable coordination of Ga-67, and therefore, the functionalization with the BBN analogues was pursued for the DOTA-containing AuNPs. The DOTA-coated AuNPs were functionalized with BBN[7-14] using a unidentate cysteine group or a bidentate thioctic group to attach the peptide. AuNPs functionalized with thioctic-BBN displayed the highest in vitro cellular internalization (approximate to 25%, 15 min) in gastrin releasing peptide (GRP) receptor expressing cancer cells. However, these results fail to translate to in vivo tumor uptake. Biodistribution studies following intravenous (IV) and intraperitoneal (IP) administration of nanoconjugates in tumor bearing mice indicated that the presence of BBN influences to some degree the biological profile of the nanoconstructs. For IV administration, the receptor-mediated pathway appears to be outweighed by the EPR effect. By contrast, in IP administration, it is reasoned that the GRPr-mediated mechanism plays a role in pancreas uptake.
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