期刊
ANALYTICAL CHEMISTRY
卷 91, 期 24, 页码 15461-15468出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b03180
关键词
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资金
- National Science Foundation of China [51773159, 51303142]
- Outstanding Young and Middle-aged Scientific Innovation Team of Colleges and Universities of Hubei Province, Biomass chemical technologies and materials [T201908]
- Science and Technology Planning Program of Guangdong [2015A010105018]
- Special Fund of Guangdong Academy of Science [2018GDASCX0105]
- Graduate Innovative Fund of Wuhan Institute of Technology [CX2017121]
High-purity cellulose membranes prepared via a green pathway were used to attempt to enhance their performance of glucose detection compared with that of traditional filter paper. In this work, cellulose-based strips (CBS) for the low concentration of glucose detection have been designed based on a fast, sensitive, and easy enzyme colorimetric assay from porous and high-purity cellulose membranes (CM). Different from the traditional paper-based sensors that were made of commercially available filter paper, the cellulose-based membranes matrix was fabricated by a green route in that cellulose was dissolved in an aqueous NaOH/urea solution, and then modified by the sodium periodate oxidation method to immobilize the glucose oxidase (GO(x) and horseradish peroxidase (HRP) with Schiff-base reaction. The structure and properties of CM and CBS were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray mapping (EDS), Fourier-transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), etc. SEM images showed a porous, interpenetrating structure of CM, which benefited the enzyme immobilization and enzymatic reaction. When glucose solution was dropped onto the CBS, the color change from colorless to blue was only 5 min. The limit of detection (LOD) is 0.45 mM in the linear range of glucose from 1 to 11 mM. Moreover, the CBS had also been successfully used for glucose determination in real urine samples, and the color changes can be easily recorded by a simple camera to achieve the semiquantitative detection of glucose. This work provided a new design strategy for the cellulose-based functional materials which could be applied in biosensors, drug carriers, and biomedicine.
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