Article
Chemistry, Analytical
Jie Zhang, Yuheng Lu, Wei Gao, Peng Yang, Nansheng Cheng, Yanwen Jin, Junbo Chen
Summary: In this study, a recognition-triggered structure switching-based element spectrometric assay was developed for ultrasensitive and highly specific detection of Ochratoxin A (OTA). The OTA aptamer was used to form a locked hairpin primer, which was then attached to magnetic beads. Upon binding of OTA to its aptamer, the primer was released, allowing for spontaneous structure-switching. Rolling circle amplification was used to produce repeated sequences by designing a dumbbell structure template. A DNA-AuNP tag was prepared to hybridize with the repeated sequences, enabling the formation of a detectable signal. This method allows for amplified detection of OTA at femtomolar concentrations and has potential applications in food safety inspection.
MICROCHEMICAL JOURNAL
(2023)
Article
Multidisciplinary Sciences
Georgia Diakoudi, Paolo Capozza, Gianvito Lanave, Francesco Pellegrini, Barbara Di Martino, Gabriella Elia, Nicola Decaro, Michele Camero, Paola Ghergo, Fabio Stasi, Alessandra Cavalli, Maria Tempesta, Vanessa R. Barrs, Julia Beatty, Krisztian Banyai, Cristiana Catella, Maria S. Lucente, Alessio Buonavoglia, Giovanna Fusco, Vito Martella
Summary: Hepadnaviruses can be found in dogs, similar to the hepadnavirus in domestic cats. Canine hepadnavirus can cause altered hepatic markers, but its role in canine health remains unclear.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Chen-Chen Li, Jinping Hu, Xiliang Luo, Juan Hu, Chun-Yang Zhang
Summary: A quantum dot-mediated fluorescence nanosensor was developed for sensitive detection of SNPs in cancer cells, offering high amplification efficiency, zero background signal, good specificity, and high sensitivity. It can detect a wide range of SNPs and accurately identify mutations as low as 0.001% in mixtures, achieving single-cell level SNP quantification.
ANALYTICAL CHEMISTRY
(2021)
Article
Environmental Sciences
Jian Meng, Qun Zhang, Min Ma, Huahong Shi, Guimei He
Summary: Plastics have been found to be colonized with pathogens and may serve as vectors for disease transmission. This study found that H9N2 avian influenza virus can persist on plastic surfaces for up to 14 days, with environmental factors such as temperature and humidity playing a significant role in virus survival and transmission. These findings highlight the importance of considering environmental factors when assessing the potential of plastics to spread viruses.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Review
Chemistry, Analytical
Lulu Xu, Jiaxin Duan, Junman Chen, Shijia Ding, Wei Cheng
Summary: RCA is an efficient enzymatic isothermal reaction using circular probes to amplify circular templates rapidly, with high specificity and the ability to generate customized functional nucleic acids for in situ detection.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Xiaoyue Zhang, Xueying Zhu, Yuanfang Li, Xin Hai, Sai Bi
Summary: In this study, a colorimetric/photothermal dual-mode exosomes sensing platform was developed for the detection of human breast cancer cell (MCF-7)-derived exosomes. The platform utilizes the encapsulation of 3,3',5,5'-tetramethylbenzidine-loaded graphene quantum dot nanozymes (TMB-GQDzymes) into DNA flowers (DFs) via rolling circle amplification (RCA). The dual-aptamer recognition strategy enables specific detection, and the platform showed excellent performance in distinguishing breast cancer patients from healthy individuals in serum samples analysis.
Article
Virology
Fatima-Zohra Sikht, Mariette Ducatez, Charifa Drissi Touzani, Adam Rubrum, Richard Webby, Mohammed El Houadfi, Nour-Said Tligui, Christelle Camus, Siham Fellahi
Summary: This study monitored and assessed the presence of H9N2 avian influenza virus in eight different regions of Morocco, and analyzed the phylogenetic and molecular evolution of the virus between 2016 and 2019. The results showed that the virus was still present in 2018-2019 regardless of vaccination status. Molecular analysis identified mutations related to virulence and increased resistance to antiviral drugs in the Moroccan H9N2 viruses.
Article
Biochemistry & Molecular Biology
Xiaowen Cao, Feng Chen, Jing Xue, Yue Zhao, Min Bai, Yongxi Zhao
Summary: Here, we present a hierarchical DNA branch assembly-encoded fluorescent nanoladders for denoised and highly multiplexed signal amplification of single-cell transcripts. Our method achieves independent RNA-primed rolling circle amplification without nonspecific amplification and allows for programmable DNA branch assembly to encode virtual signals for visualizing numbers of targets. We demonstrate accurate RNA profiling in different cancer cells and reveal diverse localization patterns for spatial regulation of transcripts.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Multidisciplinary Sciences
Gun-Soo Park, Jin-Soo Maeng
Summary: We developed a method for amplifying kilobases-long target sequence from RNA templates using Cre recombinase to generate circular intermediate template for subsequent amplification. The method can amplify a target sequence of about 2.9 kb, and the amplicon can be used for cloning.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Jingyang Zhang, Yu Wang, Xudong Zhao, Mengmeng Chen, Yuan Peng, Jialei Bai, Shuang Li, Dianpeng Han, Shuyue Ren, Kang Qin, Sen Li, Tie Han, Zhixian Gao
Summary: The development of a dual sensitization smartphone colorimetric strategy based on rolling circle amplification (RCA) coils gathering Au tetrahedra has been successful. This strategy utilizes the dual sensitization effect of RCA and Au tetrahedra to amplify the signal. The use of ImageJ software allows for timely target detection under practical conditions.
ANALYTICAL CHEMISTRY
(2021)
Article
Infectious Diseases
Mohsen Bashashati, David H. Chung, Mohammad Hossein Fallah Mehrabadi, Dong-Hun Lee
Summary: The evolutionary history of H9N2 avian influenza viruses in Iran shows that only the Iran 4 subgroup has survived and currently circulates in the country; the viruses possess molecular markers associated with mammalian adaption; continued surveillance of H9N2 viruses is needed to monitor their evolution and potential threat to public health.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Chemistry, Analytical
Yanfei Zhang, Jun Chen, Huihui Yang, Wen Yin, Chunrong Li, Yuzhi Xu, Si-Yang Liu, Zong Dai, Xiaoyong Zou
Summary: The LCR-HCR method uses exosomes as vehicles for controlled release and recruitment, significantly improving miRNA imaging contrast by confining reactants on exosome vehicles. It provides a powerful tool for sensitive miRNA imaging in living cells and cancer diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Letter
Immunology
Samnang Um, Jurre Y. Siegers, Borann Sar, Savuth Chin, Sarika Patel, Seng Bunnary, Makara Hak, Sothy Sor, Oum Sokhen, Seng Heng, Darapheak Chau, Tum Sothyra, Asheena Khalakdina, Joshua A. Mott, Sonja J. Olsen, Filip Claes, Ly Sovann, Erik A. Karlsson
Summary: In February 2021, a case of human avian influenza A(H9N2) virus infection was detected in Cambodia through routine surveillance. Investigations found no recent H9N2 virus infections among 43 close contacts. A chicken sample from the infected child's house tested positive for H9N2 virus and was genetically similar to the human virus.
EMERGING INFECTIOUS DISEASES
(2021)
Review
Chemistry, Multidisciplinary
Chi Yao, Junhan Ou, Jianpu Tang, Dayong Yang
Summary: Facing the increasing demand for precision medicine, materials chemistry systems with accurate molecular design, controllable structure, and adjustable biological activity are required for bioanalysis. In recent years, DNA has been considered a potential biomaterial for analysis and has been applied in various fields. DNA supramolecular assembly on micro/nanointerfaces is an effective strategy to concentrate functional DNA modules and enhance their functions in bioanalysis. This research direction is expected to guide the construction of more bioanalytical materials and promote the development of precision medicine.
ACCOUNTS OF CHEMICAL RESEARCH
(2022)
Article
Chemistry, Analytical
Chenxin Fang, Ping Ouyang, Yuxing Yang, Yang Qing, Jialun Han, Wenyan Shang, Yubing Chen, Jie Du
Summary: A microRNA detection platform utilizing a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system has been proposed for rapid and efficient detection of miRNA-21. This system achieves signal amplification and fluorescence intensity amplification through different mechanisms, showing broad prospects in nucleic acid analysis and detection.
Article
Biochemical Research Methods
Dandan Li, Man He, Beibei Chen, Bin Hu
JOURNAL OF CHROMATOGRAPHY A
(2019)
Article
Chemistry, Analytical
Zhenna Chen, Beibei Chen, Man He, Han Wang, Bin Hu
Article
Biochemical Research Methods
Dandan Li, Man He, Beibei Chen, Bin Hu
JOURNAL OF CHROMATOGRAPHY A
(2019)
Article
Chemistry, Analytical
Xiaoting Li, Beibei Chen, Man He, Bin Hu
Article
Chemistry, Analytical
Xiaolan Liu, Man He, Beibei Chen, Bin Hu
Article
Biochemical Research Methods
Man He, Xiaoxiao Ou, Yuxin Wang, Zhenna Chen, Dandan Li, Beibei Chen, Bin Hu
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Chemistry, Analytical
Guangyang Xiao, Beibei Chen, Man He, Xiaoting Li, Bin Hu
Article
Chemistry, Analytical
Bingshan Zhao, Man He, Beibei Chen, Bin Hu
Article
Environmental Sciences
Yin Wang, Beibei Chen, Baoshan Wang, Man He, Bin Hu
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Chemistry, Analytical
Wenying Fan, Man He, Linna You, Beibei Chen, Bin Hu
JOURNAL OF SEPARATION SCIENCE
(2020)
Article
Chemistry, Analytical
Zhenna Chen, Beibei Chen, Man He, Bin Hu
Article
Chemistry, Analytical
Man He, Shaowei Su, Beibei Chen, Bin Hu
Article
Chemistry, Analytical
Xue Ren, Man He, Beibei Chen, Bin Hu
ANALYTICAL METHODS
(2020)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)