Article
Chemistry, Multidisciplinary
Qingyi Hu, Kejun Dong, Jie Ming, Wen Yang, Hongbo Wang, Xianjin Xiao, Tao Huang
Summary: DNA hydrogels have unique properties that have led to increased research in nanomaterials and biomedical fields. However, the lack of effective methods to regulate microstructure limits their applications in tissue engineering. By introducing DNA scaffolds and implementing rapid self-assembly strategies, a regulable scaffold-net DNA hydrogel can be produced, offering a promising platform for cancer cell metastasis and microenvironment biophysics studies.
MATERIALS TODAY CHEMISTRY
(2022)
Review
Engineering, Biomedical
Kenichi Arai, Takahiro Kitsuka, Koichi Nakayama
Summary: The safety and efficacy of new drugs are typically tested in animals, but differences in drug responses and potential cardiac side effects have led to many drug development projects being discontinued. Alternative methods to animal testing are needed. Tissue engineering techniques can create three-dimensional cardiac constructs from hiPSC-CMs, providing a new approach for drug screening.
Article
Biochemistry & Molecular Biology
Zhinan Mao, Xuewei Bi, Fan Ye, Puyu Du, Xiong Shu, Lei Sun, Juan Guan, Xiaoming Li, Sujun Wu
Summary: The appropriate dosage of crosslinking agent EGDE is critical for achieving balanced mechanical properties, in vivo degradability, and immunological properties of silk fibroin scaffold platform for soft tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Physical
Stefan Jentsch, Ramin Nasehi, Christoph Kuckelkorn, Benedikt Gundert, Sanja Aveic, Horst Fischer
Summary: Bioprinting faces challenges such as nozzle limitations and high shear stress, which can lead to cell damage. A novel 3D bioprinting method based on acoustic droplet ejection (ADE) is introduced to minimize critical shear stress and enable precise printing of cell-laden structures. This multiscale acoustic bioprinting technique shows promise for creating complex and individualized 3D hydrogel structures while preserving cell viability.
Article
Biochemistry & Molecular Biology
Abdullah Al-Ani, Derek Toms, Saud Sunba, Kayla Giles, Yacine Touahri, Carol Schuurmans, Mark Ungrin
Summary: The focus of the research is on developing size-controlled RPE microtissues suitable for injection delivery to improve the survival and integration of transplanted RPE cells, while also retaining the simplicity of production and delivery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Multidisciplinary
Pedram Iranmanesh, Mohammad Gowdini, Abbasali Khademi, Mohammad Dehghani, Mehran Latifi, Naif Alsaadi, Mehran Hemati, Ramin Mohammadi, Saeed Saber-Samandari, Davood Toghraie, Afrasyab Khan
Summary: The combination of 3D bioprinting technology with novel hydrogel and bioink has led to the successful fabrication of scaffolds with unique mechanical and biological responses. The addition of naproxen coating enhances the tensile strength of the scaffolds, showing promising compatibility with the human body.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2021)
Article
Multidisciplinary Sciences
Miriam Filippi, Oncay Yasa, Roger Dale Kamm, Ritu Raman, Robert K. Katzschmann
Summary: The next frontier in robotics will be led by biohybrids, and microfluidics is essential for sustaining, improving, and scaling the architectural complexity of biological tissues. Advances in microfluidics have already transformed disease modeling and drug development, and now have the potential to impact regenerative medicine through biohybrids. By combining microfluidics with living materials, tissue perfusion and maturation can be improved, and precise patterning of sensing, processing, and control elements can be achieved.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Engineering, Biomedical
Minglei Yang, Jiafei Chen, Yi Chen, Weikang Lin, Hai Tang, Ziwen Fan, Long Wang, Yunlang She, Feng Jin, Lei Zhang, Weiyan Sun, Chang Chen
Summary: A scaffold-free modular strategy for tracheal engineering is developed, which constructs a complex tracheal substitute by laminating a cartilage sheet and a temperature-sensitive epithelium sheet. This substitute exhibits native-like structure and function, showing great clinical potential for tracheal reconstruction.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Anna Gryadunova, Jesil Kasamkattil, Max Hans Peter Gay, Boris Dasen, Karoliina Pelttari, Vladimir Mironov, Ivan Martin, Stefan Schaeren, Andrea Barbero, Olga Krupkova, Arne Mehrkens
Summary: This study demonstrates the potential of scaffold-free spheroids derived from human nasal chondrocytes for nucleus pulposus regeneration, showing good injectability and biochemical properties under suitable culture conditions, making it a promising strategy for degenerative disc disease treatment.
ACTA BIOMATERIALIA
(2021)
Article
Robotics
Xianke Dong, Sina Kheiri, Yangning Lu, Zhaoyi Xu, Mei Zhen, Xinyu Liu
Summary: Researchers have created an untethered, highly controllable living soft microrobot called RoboWorm by engineering the genetic and nervous systems of a nematode worm. By reproducing the major crawling behaviors of the worm in a controlled manner, they have achieved closed-loop regulation of movement direction and destination of single worms. This technology may advance scientific studies on the biophysics and neural basis of crawling locomotion in C. elegans and other nematode species.
Article
Chemistry, Multidisciplinary
Yurui Xu, Anwei Zhou, Weiwei Chen, Xinghai Ning
Summary: This study presents a novel bio-orthogonal engineering strategy (BIEN) to create advanced multicellular 3D tissue constructs (MTCs) using a scaffold-free approach. By harnessing the cellular biosynthetic machinery, BIEN incorporates bio-orthogonal azide reporters into cell surface glycoconjugates and assembles MTCs through a click reaction with multiarm PEG. The resulting MTCs demonstrate uniform, heterogeneous spheroids with high intercellular junction and pluripotency, simulating tumor features and improving research models.
Article
Nanoscience & Nanotechnology
Libo Zhou, Wuxia Zhang, Jinhyung Lee, Liisa Kuhn, Yupeng Chen
Summary: This study developed an injectable nanomatrix (NM) scaffold with a layer-by-layer structure inside each nanosized fiber for highly localized drug delivery and a homeostatic microenvironment, successfully generating highly organized structures via molecular self-assembly.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Multidisciplinary Sciences
Irini Gerges, Margherita Tamplenizza, Federico Martello, Stefano Koman, Giulia Chincarini, Camilla Recordati, Mariacaterina Tamplenizza, Scott Guelcher, Maurizio Crestani, Alessandro Tocchio
Summary: By adjusting the mechanical properties and biochemical cues of 3D scaffolds, soft tissue formation can be promoted, with mechanical and biochemical conditioning showing equivalent efficacy in promoting adipose tissue formation. This study opens up a new avenue for designing synthetic 3D scaffolds for the regeneration of large volumes of soft and adipose tissue.
SCIENTIFIC REPORTS
(2021)
Article
Engineering, Biomedical
Fushi Wang, Xinjie Cai, Ya Shen, Liuyan Meng
Summary: This review discusses the effects of various types of scaffold materials on cell-scaffold interaction in tissue engineering. It elaborates on the influence of the physical, chemical, mechanical, and biological characteristics of scaffold materials on cell-scaffold interaction. The review also examines the role of scaffold components, degradation process, and degraded products in cell-scaffold interactions. Furthermore, it summarizes the latest research progress on cell-scaffold interactions among various engineered tissues or organs in the oral and craniofacial region.
BIOACTIVE MATERIALS
(2023)
Article
Engineering, Biomedical
Sangil Min, David Cleveland, In Kap Ko, Ji Hyun Kim, Hee Jo Yang, Anthony Atala, James J. Yoo
Summary: By incorporating vascular endothelial growth factor (VEGF) into the vascular scaffold, improved angiogenic capability was achieved, promoting vascularization, reducing apoptosis of implanted cells, and forming hybrid renal tubule-like structures.
ACTA BIOMATERIALIA
(2021)
Article
Engineering, Biomedical
Tanchen Ren, Shan Yu, Zhengwei Mao, Changyou Gao
Article
Chemistry, Multidisciplinary
Tanchen Ren, Yelin Ni, Wang Du, Shan Yu, Zhengwei Mao, Changyou Gao
ADVANCED MATERIALS INTERFACES
(2017)
Article
Multidisciplinary Sciences
Tanchen Ren, Anne Faust, Yolandi van der Merwe, Bo Xiao, Scott Johnson, Apoorva Kandakatla, Vijay S. Gorantla, Stephen F. Badylak, Kia M. Washington, Michael B. Steketee
SCIENTIFIC REPORTS
(2018)
Article
Biochemistry & Molecular Biology
Tanchen Ren, Shan Yu, Zhengwei Mao, Sergio Enrique Moya, Lulu Han, Changyou Gao
Article
Engineering, Biomedical
Si Cheng, Xianbao Liu, Yi Qian, Miribani Maitusong, Kaixiang Yu, Naifang Cao, Juan Fang, Feng Liu, Jinyong Chen, Dilin Xu, Gangjie Zhu, Tanchen Ren, Jian'an Wang
Summary: This study introduces a double-network tough hydrogel to improve the fatigue resistance and biocompatibility of heart valves in replacement surgeries, extending their durability and demonstrating great hemocompatibility and anti-calcification properties.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Engineering, Biomedical
Yuwen Lu, Tanchen Ren, Hua Zhang, Qiao Jin, Liyin Shen, Mengqi Shan, Xinzhe Zhao, Qichao Chen, Haoli Dai, Lin Yao, Jieqi Xie, Di Ye, Tengxiang Lin, Xiaoqian Hong, Kaicheng Deng, Ting Shen, Jiazhen Pan, Mengyan Jia, Jun Ling, Peng Li, Yue Zhang, Huanan Wang, Lenan Zhuang, Changyou Gao, Jifu Mao, Yang Zhu
Summary: This study fabricated stiff polymeric microneedles with backward-facing barbs inspired by honeybee stingers and embedded them into elastomer films to produce self-interlocking microneedle patches. The barbing pattern was adjusted to increase interlocking efficiency. The microneedle patches demonstrated strong tissue adhesion and were successfully implanted onto beating hearts. They reduced cardiac wall stress and maintained left ventricular function. The microneedle patches were minimally invasive and did not require sutures or adhesives.
ACTA BIOMATERIALIA
(2022)
Article
Chemistry, Multidisciplinary
Tanchen Ren, Miribani Maitusong, Xuhao Zhou, Xiaoqian Hong, Si Cheng, Yin Lin, Junhui Xue, Dilin Xu, Jinyong Chen, Yi Qian, Yuwen Lu, Xianbao Liu, Yang Zhu, Jian'an Wang
Summary: Tissue engineering requires precise control of cell distribution in materials and structures. Current ink-based bioprinting methods face challenges in achieving universal applicability due to the specific conditions required for different inks. The Magneto-Archimedes effect based strategy offers a promising alternative by directly modulating cell locomotion without relying on inks. However, its widespread adoption has been limited by the complexity and precision of magnetic field distribution. By controlling the paramagnetic reagent concentration and magnet gaps, researchers have successfully patterned micrometer-scale cell assemblies with single or multiple cell types. This Mag-Arch patterning technique has potential applications in tissue engineering, regenerative medicine, and cell biology research.
Article
Engineering, Biomedical
Tanchen Ren, Wolfgang Steiger, Pu Chen, Aleksandr Ovsianikov, Utkan Demirci
Article
Neurosciences
Tanchen Ren, Yolandi van der Merwe, Michael B. Steketee