期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
卷 1858, 期 11, 页码 2911-2922出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2016.09.002
关键词
Stratum corneum; Ceramides; Lipid model membrane; Long-periodicity phase; Short-periodicity phase; Neutron diffraction
资金
- Helmholtz-Zentrum Berlin fur Materialien and Energie (HZB, Berlin, Germany)
- Deutsche Forschungsgemeinschaft [NE 427/30-1, DO 463/6-1]
The outermost layer of the mammalian skin, the stratum corneum (SC), is a very thin structure and realizes simultaneously the main barrier properties. The penetration barrier for xenobiotica is mostly represented by a complex lipid matrix. There is great interest in the subject of getting information about the arrangement of the lipids, which are mainly ceramides (CER), free fatty acids (FFA) and cbolesterol (CHOL.). SC lipid model membranes containing synthetically derived lipids in a non-physiological ratio were investigated. To compare the study to a former experiment, a methyl-branched ceramide [EOS] species in presence of the ultra-long chain CER[AP], CHOL and behenic acid (23/10/33/33, wt%) was applied. The membrane structure was studied using the very versatile technique of neutron diffraction. We were able to identify a long-periodicity phase (LPP) with a size of 114 A or 118 A with CER[EOS]-br in a ratio of >60 wt% of the ceramides. Furthermore, we figured out two additional coexisting short -periodicity phases (SPP) with repeat distances of 48 A and 45 A, respectively. Partial deuterations of CER[EOS]-br and CER[AP] enabled the localization of the molecules within the multiphase system. CER[EOS]-d3 was present in the LPP, but absent in both SPP. CER[AP]-d3 was determined in both short phases but not localized within the LPP. Besides, we revealed influences of humidity and time with respect to the long -periodicity phase. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据