Article
Chemistry, Multidisciplinary
Bruno Casciaro, Maria Rosa Loffredo, Floriana Cappiello, Niamh O'Sullivan, Carola Tortora, Rizwan Manzer, Sougata Karmakar, Alan Haskell, Syed K. Hasan, Maria Luisa Mangoni
Summary: Antimicrobial resistance continues to be a problem, but a newly designed peptide called KDEON WK-11 shows strong antimicrobial activity against Pseudomonas aeruginosa with low cytotoxicity and potential anti-lipopolysaccharide qualities, making it a promising agent against sepsis.
FRONTIERS IN CHEMISTRY
(2022)
Article
Engineering, Biomedical
Ziv Azoulay, Polina Aibinder, Ayala Gancz, Jacob Moran-Gilad, Shiri Navon-Venezia, Hanna Rapaport
Summary: FKF, a tripeptide with potential antimicrobial activity, forms a pure peptide hydrogel phase made of natural amino acids. The hydrogel exhibits bactericidal activity against E. coli and P. aeruginosa, and reduces P. aeruginosa contamination in a rat skin lesion model.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Sergei Y. Grishin, Pavel A. Domnin, Sergey Kravchenko, Viacheslav N. Azev, Leila G. Mustaeva, Elena Y. Gorbunova, Margarita Kobyakova, Alexey K. Surin, Maria A. Makarova, Stanislav R. Kurpe, Roman S. Fadeev, Alexey S. Vasilchenko, Victoria V. Firstova, Svetlana A. Ermolaeva, Oxana Galzitskaya
Summary: The development and testing of new antimicrobial peptides, including a unique hybrid peptide combining membrane penetrating peptide and amyloidogenic peptide segments, showed promise in inhibiting the growth of pathogens such as Pseudomonas aeruginosa. The study demonstrated antibacterial activity comparable to gentamicin sulfate, with no toxicity to eukaryotic cells, indicating the potential for new antimicrobial drug development against P. aeruginosa using these hybrid peptides.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Sandra Patricia Rivera-Sanchez, Ivan Dario Ocampo-Ibanez, Yamil Liscano, Natalia Martinez, Isamar Munoz, Marcela Manrique-Moreno, Luis Martinez-Martinez, Jose Onate-Garzon
Summary: This study investigates the interaction between a cationic antimicrobial peptide (CAMP) derived from Cecropin D-like and bacterial-like model membranes of colistin-resistant Pseudomonas aeruginosa (CRPa) strains. The experimental analysis shows that the peptide interacts with the lipids of CRPa membranes, resulting in an increase in the phase transition temperature of the lipid systems and a transition in the peptide's secondary structure.
Article
Microbiology
Adam S. Bronson, Nina S. Baggett, Matthew T. Cabeen
Summary: Pyocins, interbacterial killing complexes produced by Pseudomonas aeruginosa, are primarily regulated by the activation of RecA due to DNA damage. However, our study reveals that the deletion of xerC enhances basal pyocin expression independently of RecA and that DNA damage-induced pyocin stimulation in Delta xerC cells is RecA-independent but dependent on PrtN. Moreover, inactivation of XerC recombinase activity leads to increased pyocin production, which is fully RecA dependent. These findings highlight the importance of XerC in regulating pyocin production and suggest potential strategies for targeting noncanonical pyocin production.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Sara Masihzadeh, Mansour Amin, Zahra Farshadzadeh
Summary: This study investigated the antibiofilm activity of the WLBU2 peptide against Pseudomonas aeruginosa isolates. Results showed that WLBU2 exhibited strong inhibitory and eradication potency against MDR and carbapenem-resistant strains of P. aeruginosa, both in vitro and in vivo.
Article
Infectious Diseases
Roberto Vazquez, Antonio Domenech-Sanchez, Susana Ruiz, Julio Sempere, Jose Yuste, Sebastian Alberti, Pedro Garcia
Summary: Phage lysins are a potential alternative to antibiotics, especially for Gram-negative pathogens. In this study, a modified antimicrobial peptide derived from a phage lysin showed improved antibacterial activity, synergy with antibiotics, and low cytotoxicity.
Article
Biochemistry & Molecular Biology
Floriana Cappiello, Sudhir Verma, Xiao Lin, Isabel Y. Moreno, Bruno Casciaro, Debarun Dutta, Alison M. McDermott, Mark Willcox, Vivien J. Coulson-Thomas, Maria Luisa Mangoni
Summary: The corneal epithelium is essential for both vision and immune defense. Corneal abrasion can lead to severe eye infections, but recent research has discovered peptides that not only treat keratitis but also promote corneal wound healing, which could have potential clinical applications.
Article
Microbiology
Yun-Qi Song, Su Min Kyung, Suji Kim, Gun Kim, So Yeong Lee, Han Sang Yoo
Summary: Pseudomonas aeruginosa is a major pathogen in nosocomial infections, which are difficult to handle due to its biofilm-forming ability and carbapenem resistance. Antimicrobial peptides, such as RP557, have shown effectiveness in inhibiting biofilms. Our study assessed the effectiveness of RP557 on carbapenem-resistant P. aeruginosa planktonic cells and biofilms, providing a potential treatment option for carbapenem-resistant P. aeruginosa infection. This treatment could be helpful in managing severe nosocomial infections caused by carbapenem-resistant bacteria and improving patients' survival rates.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Li Li, Jiaxin Li, Xiaodan Yu, Ruipin Cao, Meiling Hong, Zuxian Xu, Jian Ren Lu, Yinglu Wang, Hu Zhu
Summary: This study demonstrated the potential of two newly designed antimicrobial peptides at subinhibitory concentrations as antiquorum-sensing agents against Pseudomonas aeruginosa. The peptides showed significant inhibition of QS-regulated virulence factors and biofilm formation, as well as downregulation of QS-related gene expression. The peptides induced structural alterations in the important QS-related protein LasR and bound to its hydrophobic groove.
BIOORGANIC CHEMISTRY
(2023)
Article
Infectious Diseases
Young-Min Kim, Hyosuk Son, Seong-Cheol Park, Jong-Kook Lee, Mi-Kyeong Jang, Jung Ro Lee
Summary: Biofilms are resistant to antibiotics and are a major source of persistent and recurring infections by clinically important pathogens. Researchers are developing new and effective antibiofilm agents, among which antimicrobial peptides have attracted interest. This study evaluated the effects of 10 synthetic peptides on growth inhibition, inhibition of biofilm formation, and biofilm elimination in drug-resistant Pseudomonas aeruginosa. The results showed that WIK-14 and WIKE-14 peptides could provide novel therapeutic drugs to overcome multidrug resistance in biofilm-associated infections.
Review
Biotechnology & Applied Microbiology
Maria del Mar Cendra, Eduard Torrents
Summary: This article discusses the traits of Pseudomonas aeruginosa biofilms and the clinical challenges they pose, focusing on the bacterium's ability to coexist and interact with other microorganisms. It explores infections caused by P. aeruginosa single and polymicrobial biofilms, as well as current models used to recreate them in laboratory conditions. Antimicrobial and antibiofilm strategies developed against P. aeruginosa mono and multispecies biofilms are also detailed.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Biochemistry & Molecular Biology
Hyun Kim, Ju Hye Jang, In Young Jung, Ha Rang Kim, Ju Hyun Cho
Summary: The study focuses on the development of genetically engineered probiotics capable of detecting and eliminating Pseudomonas aeruginosa in the intestines. The engineered probiotics successfully expressed and secreted a selective antimicrobial peptide in response to a specific quorum-sensing molecule of P. aeruginosa. In vitro and in vivo experiments demonstrated the ability of the engineered probiotics to inhibit P. aeruginosa growth and reduce colonization levels in mice.
Article
Pharmacology & Pharmacy
Weibin Zhang, Zhe An, Yuqi Bai, Ying Zhou, Fangyi Chen, Ke-Jian Wang
Summary: Antimicrobial resistance (AMR) is a significant global threat to human health. In this study, a novel cationic antimicrobial peptide (AMP) named Scyreptin1-30 was identified, which showed broad-spectrum antimicrobial activity against bacteria and fungi, including the clinically isolated multidrug-resistant bacteria Pseudomonas aeruginosa. Scyreptin1-30 exhibited rapid bactericidal kinetics and destroyed bacterial membranes. It also demonstrated no discernible cytotoxicity and promoted wound healing in vivo. The findings suggest that Scyreptin1-30 holds promise as an effective antibacterial agent against multidrug-resistant bacterial infections.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Biochemical Research Methods
Corrie R. Belanger, Robert E. W. Hancock
Summary: This study presents a protocol for testing physiologically relevant infection conditions in minimal inhibitory concentration (MIC) assays, which can be used to assess the susceptibility of bacterial isolates and novel antimicrobial drugs under conditions more similar to clinical infections. The protocol can be applied to most physiologically relevant liquid medium and aerobic pathogens, taking 3-4 days to complete, and may provide a more accurate assessment of the bactericidal effects of new drugs.
Article
Engineering, Environmental
Lisa Petani, Lorenz Wuehrl, Liane Koker, Markus Reischl, Jonas Renz, Ulrich Gengenbach, Christian Pylatiuk
Summary: Ozone therapy has been established in clinical applications for decades, especially in treating infections or herniated discs. The experimental setup developed enables real-time measurement of ozone concentration in water with a spectroscopic sensor, facilitating the calibration and evaluation of new ozone sensors. Results from the spectroscopic sensor and colorimetric photometer show a strong correlation in ozone concentration measurement, and the ozone decay curves measured with the experimental setup align well with previous research.
OZONE-SCIENCE & ENGINEERING
(2022)
Article
Engineering, Electrical & Electronic
Felix Berens, Stefan Elser, Markus Reischl
Summary: This article introduces a new method for optimizing the configuration of multiple sensors in autonomous vehicles, considering their position, rotation, and the impact on perception capabilities. The method takes into account the vehicle's shape, sensor size, and error assistance to ensure safe perception.
IEEE SENSORS JOURNAL
(2022)
Article
Materials Science, Multidisciplinary
Y. Lingelbach, T. Waldenmaier, L. Hagymasi, R. Mikut, V Schulze
Summary: This study explains the variance in core hardness of 18CrNi8 nozzle bodies after industrial heat treatment by aggregating data from various sources over five years. Traditional physical models and data-driven machine learning models are compared, with the latter showing no advantage due to low variability in the data. Visible drifts in hardness are best tracked by an alternative model that uses past measurements rather than by the other methods. Additionally, machine learning on features from heat treatment is not successful in predicting hardness change, possibly due to the stability of the process. Measurement error in HV 1 also contributes significantly to the variance.
MATERIALWISSENSCHAFT UND WERKSTOFFTECHNIK
(2022)
Article
Energy & Fuels
Kaleb Phipps, Sebastian Lerch, Maria Andersson, Ralf Mikut, Veit Hagenmeyer, Nicole Ludwig
Summary: This paper examines multiple strategies for applying ensemble post-processing to probabilistic wind power forecasts and finds that post-processing the final wind power ensemble improves forecast performance, while only post-processing the weather ensembles does not necessarily lead to increased forecast performance.
Article
Clinical Neurology
Farrokh Manzouri, Marc Zoellin, Simon Schillinger, Matthias Duempelmann, Ralf Mikut, Peter Woias, Laura Maria Comella, Andreas Schulze-Bonhage
Summary: This study proposes three patient-specific, energy-efficient seizure detectors and validates their applicability and energy consumption. The results show that seizure detection can be achieved using a few channels with limited spatial distribution, which has implications for designing new models for responsive neurostimulation.
FRONTIERS IN NEUROLOGY
(2022)
Article
Computer Science, Artificial Intelligence
Benedikt Heidrich, Marian Turowski, Kaleb Phipps, Kai Schmieder, Wolfgang Suess, Ralf Mikut, Veit Hagenmeyer
Summary: This paper presents a novel approach for controlling non-stationarity and periodicities in generated time series by using calendar and statistical information. The evaluation of the proposed approach shows that it can generate time series with controlled periodicities and non-stationarity, and outperforms existing benchmarks.
APPLIED INTELLIGENCE
(2023)
Article
Biochemical Research Methods
Martin Maska, Vladimir Ulman, Pablo Delgado-Rodriguez, Estibaliz Gomez-de-Mariscal, Tereza Necasova, Fidel A. Guerrero Pena, Tsang Ing Ren, Elliot M. Meyerowitz, Tim Scherr, Katharina Loeffler, Ralf Mikut, Tianqi Guo, Yin Wang, Jan P. Allebach, Rina Bao, Noor M. Al-Shakarji, Gani Rahmon, Imad Eddine Toubal, Kannappan Palaniappan, Filip Lux, Petr Matula, Ko Sugawara, Klas E. G. Magnusson, Layton Aho, Andrew R. Cohen, Assaf Arbelle, Tal Ben-Haim, Tammy Riklin Raviv, Fabian Isensee, Paul F. Jaeger, Klaus H. Maier-Hein, Yanming Zhu, Cristina Ederra, Ainhoa Urbiola, Erik Meijering, Alexandre Cunha, Arrate Munoz-Barrutia, Michal Kozubek, Carlos Ortiz-de-Solorzano
Summary: The Cell Tracking Challenge is a benchmarking initiative that has made significant improvements since 2017, including the creation of a new segmentation-only benchmark and the addition of diverse and complex datasets to the repository. A silver standard reference corpus has been created based on competitive results, which will benefit data-hungry deep learning-based strategies. This updated analysis provides practical conclusions for developers and users of cell segmentation and tracking algorithms, highlighting the advancements in both 2D and 3D algorithms.
Article
Metallurgy & Metallurgical Engineering
Elisabeth Birgit Schwarz, Fabian Bleier, Friedhelm Guenter, Ralf Mikut, Jean Pierre Bergmann
Summary: Ultrasonic metal welding is an attractive joining technology with high energy efficiency and solid-state joint formation. This study compares the suitability of contactless temperature measurements and vibration measurements for process monitoring of copper sheet welding. The results show that quality predictions based on temperatures are superior to those based on welding energy, and predictions based on both temperature and vibration measurements are even better. Therefore, combining temperature and vibration measurements is a good starting point for future industrial monitoring of ultrasonic metal welding processes.
WELDING IN THE WORLD
(2023)
Article
Mathematics, Interdisciplinary Applications
Thorbjorn Lund Onsaker, Heidi S. Nygard, Damia Gomila, Pere Colet, Ralf Mikut, Richard Jumar, Heiko Maass, Uwe Kuehnapfel, Veit Hagenmeyer, Benjamin Schaefer
Summary: Modelling, forecasting, and understanding the dynamics of the power grid and its frequency are crucial for ensuring the safe operation of power grids. This study focuses on European islands and compares their frequency prediction to the Nordic power system as a reference. The Balearic Islands are found to be more predictable, while Iceland is harder to predict. Additionally, it is shown that 2-4 weeks of data are needed for improved prediction performance.
JOURNAL OF PHYSICS-COMPLEXITY
(2023)
Article
Pharmacology & Pharmacy
Kai Hilpert, Christoph Rumancev, Jurnorain Gani, Dominic W. P. Collis, Paula Matilde Lopez-Perez, Vasil M. Garamus, Ralf Mikut, Axel Rosenhahn
Summary: Candida albicans is a common cause of candidiasis, and the development of resistance to antifungal drugs is a concern. A new method using BioSAXS was used to investigate the effects of antifungal compounds on the ultrastructure of C. albicans. The results suggest that certain antifungal peptides may have different modes of action.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Microbiology
Kai Hilpert, Tulika Munshi, Paula M. Lopez-Perez, Joana Sequeira-Garcia, Sven Hofmann, Tim J. Bull
Summary: Antimicrobial peptides can kill various microorganisms and stimulate bacterial response at sublethal concentrations. This study discovered antimicrobial peptides that can stimulate the growth of certain mycobacteria.
Article
Chemistry, Multidisciplinary
Sahana Sheshachala, Birgit Huber, Jan Schuetzke, Ralf Mikut, Tim Scharnweber, Carmen M. Dominguez, Hatice Mutlu, Christof M. Niemeyer
Summary: Microfluidic droplets are used to study and mimic biological systems. In this study, a new charged fluorosurfactant was synthesized to assemble DNA-modified silica nanoparticles at the interface of microfluidic droplets.
NANOSCALE ADVANCES
(2023)
Article
Multidisciplinary Sciences
Vojtech Kumpost, Lennart Hilbert, Ralf Mikut
Summary: Biological oscillators are affected by stochastic fluctuations and changing environmental conditions. In populations of uncoupled oscillators, noise can enable population-level entrainment for a wider range of input amplitudes and periods. This study found that increasing noise intensity in a population of a sufficient number of oscillators results in faster entrainment after a phase change of the input signal and increased sensitivity to low-amplitude input signals.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2023)
Proceedings Paper
Computer Science, Interdisciplinary Applications
Dorina Werling, Maximilian Beichter, Benedikt Heidrich, Kaleb Phipps, Ralf Mikut, Veit Hagenmeyer
Summary: Transforming the energy system to decentralised, renewable energy sources requires measures to balance their fluctuating nature and stabilise the energy system. One such measure is a dispatchable feeder, which combines inflexible prosumption with a flexible energy storage system. However, the accuracy of energy time series forecasts has a complex impact on the performance of the dispatchable feeder, thus requiring analysis of the forecast value.
E-ENERGY '23 COMPANION-PROCEEDINGS OF THE 2023 THE 14TH ACM INTERNATIONAL CONFERENCE ON FUTURE ENERGY SYSTEMS
(2023)
Article
Biochemistry & Molecular Biology
Philipp Grad, Katarina Edwards, Lars Gedda, Victor Agmo Hernandez
Summary: This study investigates the effects of polyethylene glycol-(PEG) modified lipids and gangliosides on the Ca2+ induced interaction between liposomes composed of palmitoyl-oleoyl phosphatidylethanolamine (POPE) and palmitoyl-oleoyl phosphatidylserine (POPS) at physiological ionic strength. The results show that naked liposomes tend to adhere, rupture, and collapse on each other's surfaces upon addition of Ca2+, eventually resulting in the formation of large multilamellar aggregates and bilayer sheets. However, the presence of gangliosides or PEGylated lipids leads to the formation of small, long-lived bilayer fragments/disks. PEGylated lipids seem to be more effective than gangliosides at stabilizing these structures. The study suggests that direct liposome-liposome fusion is not the dominating process triggered by Ca2+ in the systems studied.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Saurabh Saraswat, Archana Chugh
Summary: Cell penetrating peptides (CPP) are important tools for intracellular delivery of molecules. In this study, a novel marine-derived CPP from Engraulis japonicus, called Engraulisin, was reported. Engraulisin demonstrated successful cellular uptake and selective antimicrobial activity against Methicillin-resistant Staphylococcus aureus (MRSA), making it a potential candidate for drug delivery.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Emanuela Efodili, Ashlynn Knight, Maryem Mirza, Cedric Briones, Il-Hyung Lee
Summary: This study demonstrates the spontaneous transfer of small membrane-bound peptides between a supported lipid bilayer and giant unilamellar vesicles in vitro. The transfer occurs through the formation of hemi-fusion stalks and is limited to small peptides due to the highly curved structure of the stalk. This system provides a synthetic platform for studying peptide trafficking between synthetic membranes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Yueqi Niu, Si Jia Chen, Jeffery B. Klauda
Summary: Macrophage membranes in the activated state are more tightly packed, exhibit increased chain order across lipid species, and form specific lipid clusters. These findings provide physiologically accurate models for future computational studies of macrophage membranes and their proteins.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Niki Baccile, Vincent Chaleix, Ingo Hoffmann
Summary: This study measured the bending rigidity of self-assembled structures formed by a new biobased glucolipid bioamphiphile using neutron spin-echo (NSE) technology. The results showed that the bending rigidity of these structures is lower or higher compared to phospholipid membranes, providing a new theoretical basis for the application of these new molecular systems.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Alain Bolano Alvares, Pablo E. A. Rodriguez, Gerardo D. Fidelio
Summary: The behavior of amphiphilic molecules at the air/water interface was studied to evaluate the arrangement formed in a confined area. The surface properties of zwitterionic DPPC lipid and A beta(1-40) amyloid peptide in mixed films were investigated at different temperatures. DPPC undergoes a phase transition depending on temperature and lateral pressure, which allows for the study of its influence on amyloid structure formation.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Lukasz Plachta, Marzena Mach, Magdalena Kowalska, Pawel Wydro
Summary: Resveratrol, a popular phytoalexin found in grapes and red wine, has beneficial effects on the cardiovascular system, exhibits antiviral, antibacterial, and antifungal properties, and may have therapeutic effects against cancer. This study investigates the interaction of resveratrol with model cell membranes, demonstrating changes in their physicochemical parameters and highlighting the role of cholesterol content in resveratrol incorporation. The findings suggest that the molecular mechanism of action of resveratrol may involve interactions with lipid rafts.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Edurne Rujas, Beatriz Apellaniz, Johana Torralba, David Andreu, Jose M. M. Caaveiro, Shixia Wang, Shan Lu, Jose L. Nieva
Summary: This study investigates the role of fusion peptide and Trp-rich membrane proximal external region in HIV-1 fusion and demonstrates that liposome-based formulations containing FP-MPER hybrid peptides can induce the production of specific neutralizing antibodies in rabbits. The results support the use of liposomes as vaccine carriers and the inclusion of lipid membranes in immunogens to elicit specific humoral responses.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Dailin Li, Dingyuan Shi, Lei Wang
Summary: G protein-gated inwardly rectifying potassium (GIRK) channels play a significant role in regulating cell excitability. This study investigated the ion permeation mechanism in GIRK2 mutants using molecular dynamic simulations and QM/MM methods. The results revealed the importance of a multi-ion distribution for ion conduction.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Max J. den Uijl, Arnold J. M. Driessen
Summary: Membrane protein insertion into and translocation across the bacterial cytoplasmic membrane are essential processes facilitated by the Sec translocon. The phospholipid dependence of membrane protein insertion has remained mostly unknown. This study reveals that the insertion of the mannitol permease MtlA into the membrane depends on the presence of phosphatidylglycerol and is stimulated by phosphatidylethanolamine.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Patrick Allen, Adam C. Smith, Vernon Benedicto, Abbas Abdulhasan, Vasanthy Narayanaswami, Enrico Tapavicza
Summary: We explore the stability, size, and structure of nanodiscs formed by the N-terminal domain of apolipoprotein E3 and a variable number of 1,2-dimyristoyl-sn-glycero-3-phosphocholine molecules. Our simulations suggest that nanodiscs containing 240 to 420 DMPC molecules are stable. The antiparallel configuration shows more protein-protein interactions and ionic contacts, as well as greater stability and rigidity compared to the parallel configuration.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Patrick M. Keating, Nicholas P. Schifano, Xinrui Wei, Matthew Y. Kong, Jinwoo Lee
Summary: Lassa virus (LASV) infection is dependent on the fusion of its viral membrane with the host cell membrane, and our study reveals that this fusion process involves pH-dependent conformational changes in the transmembrane domain of LASV. These findings provide valuable insights into the fusion mechanism of LASV and can be utilized in the design of therapeutics to combat Lassa virus infections and prevent its potential spread.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Ashley L. Bennett, Kristen N. Cranford, Austin L. Bates, Christopher R. Sabatini, Hee-Seung Lee
Summary: This study employed molecular dynamics simulations to elucidate the binding, folding, and insertion details of TP10W in the cell membrane. The simulations revealed that TP10W can translocate across the lipid membrane as a monomer, and the charged amino acid side chains can move between lipid leaflets. Comparison with point mutated variants demonstrated significant conformational changes due to charge distribution.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Jessica H. van Wonderen, Jason C. Crack, Marcus J. Edwards, Thomas A. Clarke, Gerhard Saalbach, Carlo Martins, Julea N. Butt
Summary: Electrogenic bacteria transfer excess respiratory electrons to metal oxide particles and electrodes through the MtrCAB complex. The crystal structure of MtrCAB from S. baltica OS185 was resolved, and liquid chromatography mass spectrometry was used to determine the mass values of the three proteins in purified MtrCAB complexes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Review
Biochemistry & Molecular Biology
Yi-Ming Chen, Ching-Tai Lu, Chia-Wen Wang, Wolfgang B. Fischer
Summary: A series of dye ligands were ranked based on docking experiments, selecting the most suitable ligands for binding with different proteins. Additionally, a comparison study was conducted on a series of repurposing drugs and known antivirals.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
