Article
Biotechnology & Applied Microbiology
Rajnish Prakash Singh, Ying Ma, Afreen Shadan
Summary: The 1-aminocyclopropane-1-carboxylate deaminase (ACCD) enzyme plays a crucial role in alleviating stress in plants by reducing ethylene production. Microbial ACCD can be used as a cost-effective and environmentally friendly biocatalyst for sustainable agriculture.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Aung Htay Naing, Hui Yeong Jeong, Sung Keun Jung, Chang Kil Kim
Summary: Overexpression of the acdS gene in Petunia hybrida significantly reduced ethylene production under stress conditions, leading to greater stress tolerance in the transgenic plants. The improved stress tolerance was associated with better plant growth and physiological performance in the transgenics compared to the wild type plants. These results suggest that overexpression of acdS in ornamental plants can help overcome the detrimental effects of ethylene on plant growth under different abiotic stresses and benefit the floricultural industry.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Chang Xu, Bowei Hao, Gongling Sun, Yuanyuan Mei, Lifang Sun, Yunmei Sun, Yibo Wang, Yongyan Zhang, Wei Zhang, Mengyuan Zhang, Yue Zhang, Dan Wang, Zihe Rao, Xin Li, Qingxi Jeffery Shen, Ning Ning Wang
Summary: Genuine ACSs not only catalyze the conversion of S-adenosyl-methionine to the ethylene precursor ACC, but also widely have C-beta-S lyase activity. Analysis of ACS-like proteins from plants at different evolutionary stages suggests that the ACC-dependent pathway is uniquely developed in seed plants.
Article
Agronomy
Kuo-En Chen, You -Fu Liu, Yu -Chang Liu, Margo Sulistio, Chyi-Chuann Chen, Chun-Ta Wu
Summary: The expression levels of PgACS1 and PgACO2 are significantly lower in the nonclimacteric guava variety 'JJB', leading to the impairment of system-2 ethylene production and resulting in nonclimacteric behavior and long storability in 'JJB' fruits.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Yong Sun Moon, Sajid Ali
Summary: Plant growth-promoting rhizobacteria (PGPR) colonize the plant rhizosphere and play a crucial role in promoting plant growth and mitigating abiotic stress. ACC deaminase-producing PGPR have been extensively used for improving crop growth under various stress conditions. However, the mechanism of ACC acquisition by ACC deaminase-producing bacteria is not well understood.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Preshobha Kodackattumannil, Kenna Whitley, Shina Sasi, Geetha Lekshmi, Saranya Krishnan, Salima Al Senaani, Martin Kottackal, Khaled M. A. Amiri
Summary: A novel inducible promoter DREB1G from the Date palm was isolated and characterized, exhibiting higher expression levels compared to the widely used AtRD29A promoter under drought and salinity stress. It can be used to develop stress-tolerant transgenic plants by inducing the expression of stress-inducible genes.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Review
Plant Sciences
Jan Skalak, Katrina Leslie Nicolas, Radomira Vankova, Jan Hejatko
Summary: Plants growing in diverse environments must adapt and survive through multistep phosphorelay (MSP) signaling, balancing hormones and environmental cues, yet specific signal recognition mechanisms remain unclear. This summary analyzes the molecular mechanisms and signaling pathway for plant adaptation to stress.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Varvara Podia, Dimitris Chatzopoulos, Dimitra Milioni, Dimitrios J. J. Stravopodis, Irene Dervisi, Andreas Roussis, Kalliopi A. A. Roubelakis-Angelakis, Kosmas Haralampidis
Summary: Polyamine oxidases (PAOs) are involved in various physiological and developmental processes, as well as responses to stress conditions. This study investigated the transcriptional regulation of AtPAO3 using a GUS reporter-aided promoter deletion approach. The results revealed that the integrity of an upstream open reading frame (uORF) and a short inter-cistronic sequence in AtPAO3 is crucial for proper gene expression. Additionally, multiple environmental factors and hormonal signals were found to regulate the transcription of AtPAO3.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Zhijuan Feng, Na Liu, Guwen Zhang, Yuanpeng Bu, Bin Wang, Yaming Gong
Summary: Tonoplast intrinsic proteins (TIPs) are a sub-family of aquaporins (AQPs) that play important roles in plant abiotic stress responses. In this study, the promoter of the GmTIP1;6 gene in vegetable soybean was cloned, and its expression pattern and promoter analyses revealed its involvement in drought, salt, abscisic acid (ABA), and methyl jasmonate (MeJA) stress processes. Histochemical staining and activity assays further confirmed the high expression and responsiveness of the GmTIP1;6 promoter to different abiotic stress treatments. Overall, the GmTIP1;6 promoter can be utilized to enhance the tolerance of transgenic plants to abiotic stresses by promoting the expression of vegetable soybean AQPs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Arijit Ghosh, Indraneel Saha, Masayuki Fujita, Subhas Chandra Debnath, Alok Kumar Hazra, Malay Kumar Adak, Mirza Hasanuzzaman
Summary: The application of TiO2-NC coating extends the shelf-life of Capsicum fruit and has positive effects on its morphological, physiological, and molecular attributes.
Article
Horticulture
Shu-Zhen Jiao, Chao Guo, Wen-Kong Yao, Ning-Bo Zhang, Ji-Yuan Zhang, Wei-Rong Xu
Summary: VaHsfC1, a heat stress transcription factor from Vitis amurensis, responds to heat, cold, ABA, and salinity stress, and its overexpression in Arabidopsis enhances heat and cold tolerance. It may play a crucial role in mediating multiple abiotic stress responses in plants.
SCIENTIA HORTICULTURAE
(2022)
Article
Plant Sciences
Arun Karnwal, Seweta Shrivastava, Abdel Rahman Mohammad Said Al-Tawaha, Gaurav Kumar, Arvind Kumar, Anupam Kumar
Summary: Environmental stress produces reactive oxygen species that damage cells, leading to decreased food productivity. However, the use of plant growth-promoting bacteria (PGPR) in agriculture can mitigate the detrimental effects of biotic and abiotic stresses. PGPR improves nutrient absorption, modulates hormone levels, and enhances stress tolerance, offering a sustainable alternative to conventional farming practices. Understanding the evolutionary and ecological relationships between plants and their microbiomes is crucial for strengthening traditional agricultural techniques.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Qian Ma, Chunxue Su, Chun-Hai Dong
Summary: Quinoa, originated from South America, shows stronger salt tolerance than other crops, with ethylene and plant hormones playing important roles in its responses to salt stress. Through analysis of protein and gene expression, it was found that certain genes may be involved in regulating salt tolerance through pathways such as osmotic adjustment, cell wall organization, and plant hormone signaling.
Review
Plant Sciences
Dongdong Li, Wangshu Mou, Bram Van de Poel, Caren Chang
Summary: Research has found that ACC, a precursor of the plant hormone ethylene, can function as a signal in nonseed plants, even though it is inefficiently converted to ethylene. Distinct responses to ACC have been observed in different aspects of growth and development in liverworts and angiosperms, suggesting that ACC itself may have served as a signal before becoming the ethylene precursor in seed plants. These findings open up the possibility of uncovering a conserved ACC signaling pathway in plants and have implications for the use of ACC as a substitute for ethylene treatment in seed plants.
CURRENT OPINION IN PLANT BIOLOGY
(2022)
Article
Plant Sciences
William Errickson, Bingru Huang
Summary: This study found that a novel strain of ACCd-producing bacteria, Paraburkholderia aspalathi WSF23, can enhance the growth and stress tolerance of grass species during drought and post-drought recovery. Metabolic analysis showed that inoculation with WSF23 increased the content of metabolites related to stress defense, nitrogen metabolism, carbohydrate and pyrimidine metabolism, zeatin biosynthesis, and cellular metabolism, which contributed to improved drought tolerance and post-drought recovery in the grass species.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Chemistry, Inorganic & Nuclear
Falak Naz, Gul Asimullah Khan Nabi, Arif Nawaz, Sajid Ali, Mohsin Siddique
Summary: This study presents a novel approach utilizing potassium-doped tin oxide nanoparticles for the degradation of binary and single dyes under UV irradiation. The nanoparticles were synthesized using the co-precipitation method and characterized through various techniques. The results show that the potassium-doped tin oxide exhibits high photocatalytic degradation efficiency.
JOURNAL OF CLUSTER SCIENCE
(2023)
Article
Plant Sciences
Rafia Azmat, Iqbal Altaf, Sumeira Moin, Waseem Ahmed, Abdulwahed Fahad Alrefaei, Sajid Ali
Summary: This article discusses the accumulation of titanium dioxide nanoparticles (TiO2 NPs) in soil and its impact on plants. The uptake and accumulation of NPs in plants were observed through scanning electron microscopy and energy dispersive X-ray techniques. The results showed that NPs significantly affected the uptake of essential micro and micronutrients and enhanced photosystem II in plants.
PAKISTAN JOURNAL OF BOTANY
(2023)
Article
Microbiology
Yong-Sun Moon, Murtaza Khan, Muhammad Aaqil Khan, Sajid Ali
Summary: This study investigated the role of multi-traits Serratia fonticola (S1T1) in promoting the growth of Cucumis sativus L. under salinity stress (200 mM). The results showed that plants treated with S. fonticola (S1T1) in the root zone had significantly higher fresh and dry biomass, as well as improved chlorophyll content, compared to the control plants. Additionally, the inoculated plants exhibited increased water potential, decreased abscisic acid levels, and lower electrolytic leakage, indicating the beneficial effects of S. fonticola (S1T1) on cucumber plants under salt stress conditions. Antioxidant analysis revealed a decrease in MDA and H2O2 content, as well as an increase in catalase and superoxide dismutase activity. The symbiotic association of S. fonticola (S1T1) was also found to up-regulate the transcript accumulation of ion transporter genes, contributing to salt-stress tolerance in cucumber plants. In conclusion, the use of S. fonticola (S1T1) as a biofertilizer or microbial plant biostimulant could help alleviate the negative effects of salinity stress and improve cucumber plant growth.
Review
Biochemistry & Molecular Biology
Murtaza Khan, Sajid Ali, Tiba Nazar Ibrahim Al Azzawi, Saddam Saqib, Fazal Ullah, Asma Ayaz, Wajid Zaman
Summary: Reactive oxygen species (ROS) and reactive nitrogen species (RNS) play crucial roles as signaling molecules in the cell systems of plants, especially under stress conditions. They control plant growth, development, symbiotic association, and defense mechanisms in response to both biotic and abiotic stresses, but excessive accumulation of ROS and RNS can lead to cell damage and hinder plant growth. This review focuses on the production and elimination of ROS and RNS, their functions as signaling molecules during plant-microbe interactions, and the antioxidant system as a balancing mechanism.
Article
Agronomy
Luqman Muhammad, Salahuddin Salahuddin, Asif Khan, Yunwei Zhou, Miao He, Abdulwahed Fahad Alrefaei, Murtaza Khan, Sajid Ali
Summary: The tolerance response of four chrysanthemum cultivars to different cadmium concentrations was investigated. The study found that cadmium significantly inhibited chlorophyll and carotenoid synthesis in all cultivars. Furthermore, ultrastructure changes were observed in the cells under cadmium stress.
Review
Biochemistry & Molecular Biology
Murtaza Khan, Sajid Ali, Tiba Nazar Ibrahim Al Azzawi, Byung-Wook Yun
Summary: Nitric oxide (NO), a colorless gaseous molecule, plays a crucial role in plant growth, development, and responses to biotic and abiotic stresses. It interacts with reactive oxygen species, antioxidants, melatonin, and hydrogen sulfide, regulates gene expression, and modulates phytohormones. However, the key enzyme in NO production, nitric oxide synthase, has been poorly understood recently in both model and crop plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Sajid Ali, Nazma Faqir, Falak Naz, Muhammad Ishtiaq Jan, Naeem Khan, Amal Alotaibi, Riaz Ullah
Summary: The study found that complexes 1 and 2 can effectively inhibit the formation of bacterial biofilms and cause damage to the bacterial membrane. The minimum inhibitory concentration and minimum bactericidal concentration of complexes 1 and 2 were 46.87 +/- 1.822 and 93.75 +/- 1.345 and 47.87 +/- 1.345 and 94.85 +/- 1.466 mu g/mL, respectively. Both complexes also showed good interactions with E. coli DNA. Therefore, complexes 1 and 2 are good antibiofilm agents that can restrict the development of bacterial biofilms on therapeutic implants by disrupting the bacterial membrane and interacting with bacterial DNA.
Article
Biochemistry & Molecular Biology
Abdur Rahman, Gauhar Rehman, Nasrullah Shah, Muhammad Hamayun, Sajid Ali, Abid Ali, Said karim Shah, Waliullah Khan, Muhammad Ishaq Ali Shah, Abdulwahed Fahad Alrefaei
Summary: Green synthesis of silver nanoparticles (AgNPs) using Tribulus terrestris seed extract as the reducing and stabilizing agent was investigated for its anti-diabetic properties. The synthesized TT-AgNPs were characterized using FTIR, XRD, SEM, and UV-Vis spectroscopy. The spectroscopic analysis confirmed the development of TT-AgNPs, and SEM revealed their loose clustering and spherical structure. The in vitro assays demonstrated the high anti-diabetic activity of TT-AgNPs, and in vivo studies on streptozotocin-induced diabetic mice showed significant improvements in blood sugar levels and histological analysis of pancreatic and liver samples. These findings indicate the effective anti-diabetic properties of biosynthesized TT-AgNPs.
Article
Agronomy
Nighat Seema, Muhammad Hamayun, Anwar Hussain, Mohib Shah, Muhammad Irshad, Muhammad Qadir, Amjad Iqbal, Abdulwahed Fahad Alrefaei, Sajid Ali
Summary: The aim of this study was to isolate endophytes from the roots of Rhazya stricta and evaluate their potential in improving the growth of drought-stressed sunflower seedlings. The isolated endophytic fungus was found to enhance the growth attributes of rice varieties and contribute to the improvement of sunflower growth under drought stress. The fungal strain was identified as Fusarium proliferatum and its beneficial effects were attributed to the regulation of phytohormones and stress-related metabolites.
Article
Agronomy
Muhammad Musa, Farzana Gul Jan, Muhammad Hamayun, Gul Jan, Sumera Afzal Khan, Gauhar Rehman, Sajid Ali, In-Jung Lee
Summary: Heavy metal contamination in soil and accumulation in plants pose a serious threat to crops and human health. Endophytic fungi can enhance the plant's ability to tolerate stressful conditions. The present study found that the endophytic fungus Paecilomyces lilacinus (MRF) isolated from the roots of Justicia adathoda has the capability to alleviate heavy metal stress and improve the growth and physiological characteristics of tomatoes.
Article
Biochemistry & Molecular Biology
Tiba Nazar Al Azzawi, Murtaza Khan, Bong-Gyu Mun, Sang-Uk Lee, Muhammad Imran, Adil Hussain, Nkulu Kabange Rolly, Da-Sol Lee, Sajid Ali, In-Jung Lee, Byung-Wook Yun
Summary: This study investigated the role of AtNIGR1 in the growth and immunity of Arabidopsis thaliana. Results revealed that AtNIGR1 negatively regulated both growth and immunity processes in plants.
Article
Microbiology
Nabeela Nabeela, Sumera Afzal Khan, Saadat Mehmood, Sohaib Bin Shabbir, Sajid Ali, Abdulwahed Fahad Alrefaei, Mohammed Fahad Albeshr, Muhammad Hamayun
Summary: Industrial effluents cause water pollution and impact aquatic and terrestrial life. This study isolated efficient fungal strains, identified as Aspergillus fumigatus (SN8c) and Aspergillus terreus (SN40b), from the aquatic environment. These isolates were chosen for their ability to efficiently decolorize and detoxify Remazol brilliant blue (RBB) dye, commonly used in various industries. Among 70 different fungal isolates screened, 19 demonstrated dye decolorization capabilities, with SN8c and SN40b showing the highest decolorization capabilities in liquid medium. The study showed the potential of these indigenous aquatic fungi to restore contaminated sites and support aquatic and terrestrial life.
Article
Plant Sciences
Chrizostom Julius Niyoifasha, Birhanu Miressa Borena, Irasapa Tanimu Ukob, Phan Ngoc Minh, Tiba Nazar Ibrahim Al Azzawi, Muhammad Imran, Sajid Ali, Anousone Inthavong, Bong-Gyu Mun, In-Jung Lee, Murtaza Khan, Byung-Wook Yun
Summary: The application of sodium nitroprusside (SNP) can improve the heavy metals tolerance of rice by reversing the toxic effects of heavy metals stress and enhancing antioxidant enzyme activities. This study provides insights into the potential use of SNP as a regulator to improve rice growth and development under heavy metals stress.
Article
Biology
Naila Shah, Muhammad Irshad, Anwar Hussain, Muhammad Qadir, Waheed Murad, Asif Khan, Muhammad Awais, Abdulwahed Fahad Alrefaei, Sajid Ali
Summary: Copper is a vital micronutrient for plant growth and development, but its accumulation in soil negatively affects crop agronomic characteristics and yield. The combination of EDTA and IAA improved sunflower's ability to hyperaccumulate copper. Sunflowers treated with different levels of copper (0 (control), 25, 50, and 75 mg/kg of soil) and EDTA alone or with IAA showed significantly enhanced copper absorption and accumulation. Copper reduced root and shoot growth, impaired chlorophyll content, hormones, flavonoids, phenolics, and antioxidant response. EDTA alone reduced the harmful effects of copper, and the addition of IAA restored shoot and root growth. Higher levels of chlorophyll content, GA(3), endogenous IAA, and flavonoids were also observed, indicating improved plant health. The catalase and ascorbate peroxidase activities were restored in plants treated with EDTA and IAA. These findings suggest that EDTA and IAA can enhance sunflower's copper absorption potential and tolerance, making it a potential phytoextraction technique for copper-contaminated soil.
Review
Biology
Uzma Salam, Shakir Ullah, Zhong-Hua Tang, Ahmed A. Elateeq, Yaseen Khan, Jafar Khan, Asif Khan, Sajid Ali
Summary: Several abiotic factors, such as drought, salinity, cold, heat, ultraviolet radiations, reactive oxygen species, trace metals, and soil pH, have a destructive impact on crop yield. These factors induce physiological and biochemical changes in plants and stimulate multi-gene responses, altering the accumulation of primary and secondary metabolites. Metabolomics plays a crucial role in interpreting plant stress tolerance, with primary metabolites serving as osmolytes and osmoprotectants, and secondary metabolites acting as defenses against biotic factors and enhancing resistance against abiotic factors.