Article
Agronomy
Ranran Wu, Qinxue Zhang, Yun Lin, Jingbin Chen, Prakit Somta, Qiang Yan, Chenchen Xue, Jinyang Liu, Xin Chen, Xingxing Yuan
Summary: Marker-assisted backcross (MABC) breeding was used to develop advanced inbred lines with resistance to bruchids. The line R67-22 showed high resistance and excellent agronomic traits, indicating its potential as a new mung bean variety.
Article
Agronomy
Xiaochen Zheng, Jianian Zhou, Min Zhang, Wenjing Tan, Chunhua Ma, Ran Tian, Qiong Yan, Xin Li, Chongjing Xia, Zhensheng Kang, Xianming Chen, Xinli Zhou, Suizhuang Yang
Summary: In this study, the wheat gene Yr39 was successfully transferred into four Chinese elite wheat cultivars, resulting in the development of 11 selected lines with stripe rust resistance and desirable agronomic traits. These lines offer potential for further testing in different wheat production regions and can serve as germplasm resources for breeding new wheat cultivars with durable stripe rust resistance, high yield, and adaptation to different production environments.
Article
Biochemistry & Molecular Biology
Jae-Eun Oh, Ji-Eun Kim, Jangmi Kim, Myoung-Hee Lee, Keunpyo Lee, Tae-Ho Kim, Sung-Hwan Jo, Jeong-Hee Lee
Summary: This study developed a genome-wide set of KASP markers for marker-assisted recurrent selection in the tetraploid minor crop perilla. By using genotyping-by-sequencing to construct high-resolution linkage maps of two F2 populations, 96 genome-wide markers were selected for MARS. The accuracy of markers was confirmed using a high-throughput Fluidigm system, providing a foundation for marker-assisted backcrossing and the development of new varieties of perilla.
MOLECULAR GENETICS AND GENOMICS
(2023)
Review
Forestry
Dario Grattapaglia
Summary: GS has rapidly evolved from a hyped topic to a leading area of applied research in tree breeding, surpassing the initial phases of the hype cycle and moving towards wide application. By integrating modern DNA typing, quantitative genetics, and mixed-model analysis, GS has revolutionized the breeding approach by predicting complex phenotypes through genomic effects, leading to enhanced genetic gain and breeding value accuracy in forest trees.
Article
Plant Sciences
Vincent P. Reyes, Rosalyn B. Angeles-Shim, Merlyn S. Mendioro, Ma Carmina C. Manuel, Ruby S. Lapis, Junghyun Shim, Hidehiko Sunohara, Shunsaku Nishiuchi, Mayumi Kikuta, Daigo Makihara, Kshirod K. Jena, Motoyuki Ashikari, Kazuyuki Doi
Summary: This study successfully introgressed and stacked major yield QTLs Gn1a and WFP into selected NERICA cultivars using marker-assisted backcross breeding, leading to improvements in rice yield traits. The combination of marker-assisted selection and phenotypic evaluation at BC3F4 and BC3F5 generations effectively selected lines with target alleles and traits. Genotyping-by-sequencing validated the introgression and demonstrated the potential of utilizing yield QTLs and marker-assisted selection to improve rice cultivars.
Article
Plant Sciences
Jignesh H. Kamdar, Mital D. Jasani, Ajay B. Chandrashekar, Pasupulati Janila, Manish K. Pandey, John J. Georrge, Rajeev K. Varshney, Sandip K. Bera
Summary: In this study, high oleic acid peanut mutant alleles were introgressed into two popular peanut cultivars through markers-assisted selection and backcrossing. The resulting lines showed significantly increased oleic acid content without affecting kernel mineral and vitamin composition. The introgression lines with improved oleic acid content and high pod yield potential could be released for commercial cultivation.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Agronomy
Liqiang Song, Ruihui Wang, Xueju Yang, Aimin Zhang, Dongcheng Liu
Summary: Wheat, an essential cereal crop, provides a significant amount of calories and proteins consumed by humans. Wheat breeders face challenges in developing new varieties to meet production requirements. Marker-assisted selection (MAS) has advantages in terms of time-saving, cost-effectiveness, and goal orientation. This review provides updated information on different molecular markers and their potential applications in wheat breeding programs.
Article
Agronomy
Yong-Pei Wu, Yu-Chi Chang, Su-Chen Kuo, Dah-Jing Liao, Ting-Yu Shen, Hsin- Kuo, Sheng-Wen Wang, Yu-Chien Tseng
Summary: Sorghum is an important cereal crop with drought resistance and environmental adaptability. This study successfully utilized the three-line method to breed high-quality waxy sorghum with favorable agronomic traits and yield quality.
Article
Plant Sciences
Partha Saha, Chandrika Ghoshal, Namita Das Saha, Aakriti Verma, Mohita Srivastava, Pritam Kalia, Bhoopal Singh Tomar
Summary: Cauliflower, a widely grown cool season vegetable in India, was successfully bred with resistance genes against black rot and downy mildew through marker-assisted backcross breeding. The newly developed lines showed high resistance to diseases, increased yield, and higher vitamin C content compared to the original cultivar. The introgression of resistant genes benefits farmers by reducing input costs and consumers by providing chemical residue-free produce.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Muhammad Sabar, Shahzad Amir Naveed, Shahid Masood Shah, Abdul Rehman Khan, Muhammad Musaddiq Shah, Tahir Awan, Muhammad Ramzan Khan, Zaheer Abbas, Muhammad Arif
Summary: Drought is a major threat to rice production, and the Super Basmati variety in Pakistan has been experiencing a decline in yield due to decreasing irrigation water. In this study, three novel QTLs for drought tolerance were incorporated into Super Basmati using marker assisted selection. The selected individuals with homozygous target QTLs and maximum background of Super Basmati were found to have higher yields under drought stress conditions. Six superior lines with drought tolerance and similar traits to Super Basmati were identified for release as drought-tolerant varieties or for use in breeding programs.
PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Agronomy
Dalasanuru Chandregowda Manjunathagowda, Raman Selvakumar
Summary: This study identified the male-fertility restoration locus among onion breeding lines through genotyping, using two markers with different application levels across the breeding lines. The research revealed the importance of molecular markers in marker-assisted selection for the development of F-1 hybrid onions.
GENETIC RESOURCES AND CROP EVOLUTION
(2021)
Article
Agronomy
Kartik Chandra Pradhan, Soumya Ranjan Barik, Shibani Mohapatra, Deepak Kumar Nayak, Elssa Pandit, Binod Kumar Jena, Sushree Sangeeta, Abhijit Pradhan, Abhishek Samal, Jitendiya Meher, Lambodar Behera, Debabrata Panigrahi, Arup Kumar Mukherjee, Sharat Kumar Pradhan
Summary: The bacterial blight disease of rice can be significantly reduced by transferring two BB resistance genes into susceptible rice varieties. By using marker-assisted backcross breeding, 16 progenies with desired BB resistance genes were obtained in the BC2F1 generation. Five pyramided lines carrying two resistance gene combinations exhibited enhanced resistance against BB pathogens.
Article
Agriculture, Multidisciplinary
Jean Fausto de Carvalho Paulino, Caleo Panhoca de Almeida, Isabella Laporte Santos, Joao Guilherme Ribeiro Goncalves, Sergio Augusto Morais Carbonell, Alisson Fernando Chiorato, Luciana Lasry Benchimol-Reis
Summary: This study aimed to develop bean varieties with durable resistance to diseases through assisted backcrossing. Both phenotypic and molecular selection methods were used. The results showed that selecting genotypes closest to the resistant parent in the progeny reduced the number of backcrossing cycles and facilitated early selection of resistant cultivars. The selected lines exhibited high resistance to anthracnose and other diseases, as well as desirable quality traits.
Article
Agronomy
Denia Pires de Almeida, Eveline Teixeira Caixeta, Karoliny Ferreira Moreira, Antonio Carlos Baiao de Oliveira, Katia Nogueira Pestana de Freitas, Antonio Alves Pereira, Renato Domiciano Silva Rosado, Laercio Zambolim, Cosme Damiao Cruz
Summary: By converting AFLP markers to SCAR and CAPS markers, this study developed molecular markers for breeding resistant coffee cultivars, leading to increased efficiency in selection of resistant plants.
Article
Agronomy
Goutam Das, Banshidhar Pradhan, Debendranath Bastia, Sanghamitra Samantaray, Debarchana Jena, Diptibala Rout, Paduranga Bhagwan Arsode, Vineeta Singh, Arup Kumar Mukherjee, Chander Mohan, Ramlakhan Verma
Summary: This study demonstrates the utility of marker-assisted backcross-breeding in improving submergence tolerance and bacterial blight resistance, and successfully develops sustainable rice varieties by introgressing tolerant genes and disease-resistant genes.
Article
Plant Sciences
Manmohan Dhkal, Abhishek Sharma, Gurpreet Kaur
JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Plant Sciences
Gurpreet Kaur, Mamta Pathak, Deepak Singla, Abhishek Sharma, Parveen Chhuneja, Navraj Kaur Sarao
Summary: Through hybridization to obtain the F-4 mapping population for genetic mapping of YMD resistance in bitter gourd, three QTLs associated with YMD resistance were identified on chromosomes 3, 4, and 5, providing valuable information for fine-mapping and marker-assisted selection for disease resistance in the future.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agronomy
Manmohan Dhkal, Abhishek Sharma, Sat Pal Sharma
Summary: This study identified a new begomovirus, tomato leaf curl Palampur virus (ToLCPalV), infecting muskmelon crop and conducted resistance screening on muskmelon genotypes. The results revealed a muskmelon genotype with monogenic resistance against the virus.
Article
Multidisciplinary Sciences
Ikbalpreet Singh, Ramandeep Kaur, Ashok Kumar, Satnam Singh, Abhishek Sharma
Summary: Research suggests that female whiteflies are more efficient vectors of Cotton leaf curl virus compared to males, possibly due to differences in the density of endosymbiont Arsenophonus and the expression of midgut protein genes.
Article
Agronomy
Sandeep Kaur Dhaliwal, Ranjit Kaur Gill, Abhishek Sharma, Amandeep Kaur, Dharminder Bhatia, Satinder Kaur
Summary: In this study, a large-effect QTL for resistance to Mungbean yellow mosaic India virus (MYMIV) was identified in blackgram variety Mash114 using QTL-seq approach. The researchers also identified a 500 kb genomic region closely associated with MYMIV using KASP markers. These findings will assist in marker-assisted transfer of this region into Vigna species infected by MYMIV.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Agronomy
Amanpal Kaur Sandhu, Kousik Mandal, Smriti Sharma, Sanjay Kumar Sahoo, Abhishek Sharma
Summary: This study conducted a field trial to investigate the dissipation of trifloxystrobin and tebuconazole on okra after applying Nativo. The results showed that the residues of tebuconazole were below the limit of quantification after 7 and 10 days, and trifloxystrobin dissipated within 5 days at recommended and double dosages. The risk assessment indicated that the theoretical maximum residue contributions were lower than the maximum permissible intake at both recommended and double dosages.
COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS
(2023)
Article
Agronomy
Neha Verma, Karmvir Singh Garcha, Madhu Sharma, Abhishek Sharma, Ajmer Singh Dhatt
Summary: Knowledge of gene actions governing begomovirus resistance and plant architectural traits is essential for successful hybrid breeding. In this study, gene actions associated with these traits were examined in two intervarietal crosses of Cucurbita moschata. Generation mean analysis revealed significant differences in all traits and significant variation between parental lines for most traits. The gene effects varied by trait and cross. The findings suggest that both additive effects and non-allelic interactions play important roles in these traits. This information will be valuable for the development of a breeding program for improved virus resistance and yield traits in pumpkins.
CZECH JOURNAL OF GENETICS AND PLANT BREEDING
(2023)
Article
Horticulture
Tejpal Singh Sran, Salesh Kumar Jindal, Abhishek Sharma, Neena Chawla
Summary: Pepper/Chilli leaf curl virus disease is a destructive disease that reduces pepper production. New resistant sources have been identified and their genetic characteristics have been confirmed. Resistant plants show higher levels of biochemical parameters and antioxidative compounds.
SCIENTIA HORTICULTURAE
(2023)
Article
Agronomy
Tejpal Singh Sran, Salesh Kumar Jindal, Abhishek Sharma, Dharminder Bhatia
Summary: Marker-assisted selection was used to introgress the leaf curl virus disease (LCVD) resistant gene from hot pepper to heat-tolerant bell pepper lines. Successful development of six improved LCVD resistant, heat-tolerant, and non-pungent populations was achieved through hybridization and phenotypic selection, which will contribute to future bell pepper breeding programs.
Article
Plant Sciences
Neha Verma, Karmvir Singh Garcha, Abhishek Sharma, Madhu Sharma, Dharminder Bhatia, Jiffinvir Singh Khosa, Barinder Kaur, Parveen Chuuneja, Ajmer Singh Dhatt
Summary: The resistant source PVR-1343 has been identified against mixed infection of squash leaf curl China virus and tomato leaf curl New Delhi virus. The genomic region qMI-Sq/To7.1 on chromosome 7 and qMI-Sq/To17.1 on chromosome 17 were identified as associated with resistance. The KASP marker Cmo4018182 within qMI-Sq/To7.1 accurately predicted disease reaction in diverse Cucurbita genotypes.
Article
Agronomy
Gagandeep Singh, Mamta Pathak, Abhishek Sharma
Summary: The study aimed to identify resistant sources against the yellow vein mosaic disease in okra through artificial screening. Sixteen accessions from seven different species and two cultivated okra cultivars were evaluated for their resistance to the disease. Several accessions showed high resistance, while others were susceptible to the disease. The resistance was confirmed using Deng universal primer.
Article
Plant Sciences
Sandeep Kaur Dhaliwal, Ranjit Kaur Gill, Abhishek Sharma, Sarabjit Kaur, Rohtas Singh, Satinder Kaur
Summary: This study examined the transcriptomic dynamics in blackgram genotypes with varying resistance to Mungbean yellow mosaic India virus (MYMIV). The differentially expressed genes were predominantly related to defense mechanisms and photosynthesis. The study identified four major genes with potential roles in disease resistance against MYMIV and their expression patterns varied between resistant and susceptible plants.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Sukhjeet Kaur, Salesh K. Jindal, Abhishek Sharma, Rupeet Gill
Summary: To mitigate the impact of root-knot nematode and late blight disease on tomato yield, disease-resistant indeterminate lines were developed and evaluated. Eight lines showed promise in resisting both pathogens, six lines exhibited resistance against nematode and possessed the Mi 1.2 gene, and three lines displayed high resistance to late blight disease along with multiple disease resistance and superior horticultural traits.
INDIAN JOURNAL OF GENETICS AND PLANT BREEDING
(2023)
Article
Entomology
Manmohan Dhkal, Abhishek Sharma, Sat Pal Sharma
Summary: Among the biostimulants tested, spraying buttermilk at 20% concentration was found to be the most effective in managing viral diseases in muskmelon, showing the lowest disease incidence (50.8%) and severity (21.3%). Other treatments were also significantly effective.
EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL
(2022)
Article
Agronomy
Navkiran Kaur Ludhar, Asmita Sirari, T. S. Bains, Abhishek Sharma, S. A. H. Patel, Pankaj Sharma, Upasana Rani, Harpreet Kaur Cheema
Summary: The Mungbean yellow mosaic virus was found to be prevalent in both summer and kharif season causing Yellow Mosaic Disease of mungbean. Interspecific derivative lines showing stable resistance were selected and confirmed using artificial inoculation technique, with SML 1827 showing the highest yield among all derivatives. Specific segments from rice bean were also identified in SML 1827.
