期刊
STEM CELL REPORTS
卷 13, 期 3, 页码 559-571出版社
CELL PRESS
DOI: 10.1016/j.stemcr.2019.07.010
关键词
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资金
- National Basic Research Program of China [2016YFC0902102, 2015CB910402]
- National Basic Research Program [2011CB504200, 2015CB910403]
- National Natural Science Foundation of China [81401837, 81671446, 81471066, 81261120555, 31200878, 31071875, 81271742, 31401012, 31730017]
- Science and Technology Commission of Shanghai Municipality [19140900400, 14430712100]
- Shanghai Rising-Star Program [16QA1401500]
- Shanghai Natural Science Foundation [17ZR1407900, 16ZR1410000, 12ZR1409300, 14ZR1411400]
Development of spermatogonia and spermatocytes are the critical steps of spermatogenesis, impacting on male fertility. Investigation of the related regulators benefits the understanding of male reproduction. The proteasome system has been reported to regulate spermatogenesis, but the mechanisms and key contributing factors in vivo are poorly explored. Here we found that ablation of REG gamma, a proteasome activator, resulted in male subfertility. Analysis of the mouse testes after birth showed there was a decreased number of PLZF(+) spermatogonia and spermatocytes. Molecular analysis found that REG gamma loss significantly increased the abundance of p53 protein in the testis, and directly repressed PLZF transcription in cell lines. Of note, allelic p53 haplodeficiency partially rescued the defects in spermatogenesis observed in REG gamma-deficient mice. In summary, our results identify REG gamma-p53-PLZF to be a critical pathway that regulates spermatogenesis and establishes a new molecular link between the proteasome system and male reproduction.
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