Article
Biochemistry & Molecular Biology
Sung-il Park, Hyun-Bin Kim, Hyun-Ji Jeon, Hyeran Kim
Summary: In this study, different Agrobacterium strains were used for pepper transformation, along with optimization of PPT concentration and CRISPR/Cas9 binary vector. The results demonstrate differential sensitivity of CM334 and Dempsey to Agrobacterium-mediated callus induction, as well as differential selection pressure of PPT through the pBAtC binary vector.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Van Schepler-Luu, Coline Sciallano, Melissa Stiebner, Chonghui Ji, Gabriel Boulard, Amadou Diallo, Florence Auguy, Si Nian Char, Yugander Arra, Kyrylo Schenstnyi, Marcel Buchholzer, Eliza P. I. Loo, Atugonza L. Bilaro, David Lihepanyama, Mohammed Mkuya, Rosemary Murori, Ricardo Oliva, Sebastien Cunnac, Bing Yang, Boris Szurek, Wolf B. Frommer
Summary: Bacterial leaf blight (BB) of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), poses a threat to global food security and the livelihood of small-scale rice producers. Despite robust global rice trade, Xoo strains from different regions show complete continental segregation. Unprecedented BB outbreaks were discovered in Tanzania, caused by Xoo strains carrying Asian-type TAL effectors and iTAL effectors, unlike endemic African Xoo strains. In order to protect African rice production from this emerging threat, a hybrid CRISPR-Cas9/Cpf1 system was developed to edit TALe-binding elements in SWEET promoters. The edited lines displayed broad-spectrum resistance against Asian and African Xoo strains.
Article
Plant Sciences
Lauren Dickinson, Wenxin Yuan, Chantal LeBlanc, Geoffrey Thomson, Siyuan Wang, Yannick Jacob
Summary: During transformation via Agrobacterium tumefaciens, the sequence composition of T-DNA affects its concatenation in Arabidopsis, and the loss of specific DNA repair proteins dramatically decreases T-DNA concatenation levels. T-DNA concatenation can be used as a tool to enhance gene editing.
Article
Plant Sciences
Lauren Dickinson, Wenxin Yuan, Chantal LeBlanc, Geoffrey Thomson, Siyuan Wang, Yannick Jacob
Summary: The inclusion of retrotransposon sequences in T-DNA can significantly increase the copy number of T-DNA in Arabidopsis. This increase is primarily induced by the activity of retrotransposon sequences, and T-DNA concatenation depends on the activity of DNA repair proteins. This study demonstrates the potential of T-DNA concatenation as a tool to enhance gene editing.
Article
Plant Sciences
Tongxu Xin, Haojie Tian, Yalin Ma, Shenhao Wang, Li Yang, Xutong Li, Mengzhuo Zhang, Chen Chen, Huaisong Wang, Haizhen Li, Jieting Xu, Sanwen Huang, Xueyong Yang
Summary: In this study, an efficient genetic transformation system for melon and squash was established using an optimal infiltration intensity strategy. The power of this method was harnessed to create alleles that resulted in a compact plant architecture with shorter internodes in melon, squash, and cucumber.
HORTICULTURE RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Matthias Knoedler, Katharina Frank, Lucy Kerpen, Johannes Felix Buyel
Summary: Antibody-drug conjugates (ADCs) have higher therapeutic indices compared to plain monoclonal antibodies (mAbs), but are more complex and costly to synthesize. Recombinant immunotoxins (RITs) consisting of antibody derivatives, linkers, and proteinaceous toxins can be produced more easily in plants. In this study, effective RITs targeting CD64 for the treatment of myelomonocytic leukemia were produced in Nicotiana benthamiana and N. tabacum plants and tobacco BY-2 cell-based plant cell packs (PCPs). The accumulation of RITs was optimized to a high yield using a design of experiments approach and an optimized affinity chromatography method. One RIT candidate targeting CD64 was characterized for storage stability and cytotoxicity. Bottlenecks in the plant-based expression platform were identified and critical process parameters for plant-made RITs were assessed.
Article
Plant Sciences
Stijn Aesaert, Lennert Impens, Griet Coussens, Els Van Lerberge, Rudy Vanderhaeghen, Laurence Desmet, Yasmine Vanhevel, Shari Bossuyt, Angeline Ndele Wambua, Mieke Van Lijsebettens, Dirk Inze, Ellen De Keyser, Thomas B. Jacobs, Mansour Karimi, Laurens Pauwels
Summary: This study made a breakthrough in plant transformation by co-transforming morphogenic transcription factors to induce somatic embryogenesis. It also showed that modifying tissue culture media and using specific constructs and control systems can reduce undesirable phenotypes and increase transformation efficiency and gene editing functionality.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Mengdou Ding, Chun-Lan Piao, Xinyu Zhang, Ying Zhu, Min-Long Cui
Summary: Solanum nigrum is an important plant with potential as a model for studying secondary metabolism and fruit development. The study reports an efficient tissue culture, transformation, and genome editing method for S. nigrum, which enables genetic integration and manipulation. This system provides a powerful platform for studying the molecular mechanisms of secondary metabolism, fruit development, and biotechnological production of important compounds.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Biotechnology & Applied Microbiology
Matias Nicolas Gonzalez, Gabriela Alejandra Massa, Mariette Andersson, Cecilia Andrea Decima Oneto, Helle Turesson, Leonardo Storani, Niklas Olsson, Ann-Sofie Falt, Per Hofvander, Sergio Enrique Feingold
Summary: Comparisons were made between different delivery methods of CRISPR/Cas9 components for potato genome editing, with transient CR-PPO expression in protoplasts showing the highest efficiency and successfully editing all four StPPO2 alleles. The dual-sgRNA strategy resulted in a low incidence of targeted deletion, potentially due to contrasting efficiencies between sgRNAs. These findings suggest that the CRISPR/Cas9 delivery strategy plays a crucial role in gene editing efficiency in potato.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Plant Sciences
Artur Pinski, Alexander Betekhtin
Summary: Fagopyrum tataricum is an exceptional crop known for its health benefits and gluten-free properties. Through optimized genetic transformation, the study demonstrates the feasibility of trait enhancement in F. tataricum.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Qianqian Li, Shuang Liu, Xuxia He, Haiyun Li, Shanhua Lyu, Yinglun Fan
Summary: This study established a highly efficient Agrobacterium rhizogenes-mediated genetic transformation method in Limonium bicolor and successfully established a CRISPR/Cas9 system. These methods provide valuable tools for functional genomics investigation and trait improvement in Limonium bicolor.
Article
Biology
Dandan Liu, Qian Liu, Wenzhu Guo, Yin Liu, Min Wu, Yongli Zhang, Jingen Li, Wenliang Sun, Xingji Wang, Qun He, Chaoguang Tian
Summary: In this study, efficient transformation tools were developed for A. niger N1 and O1, and a marker-free CRISPR/Cas9 genome editing system was successfully constructed. Nine mutants were generated using these tools. These genetic tools provide an important reference for manipulating tough working industrial fungi.
Article
Plant Sciences
J. E. Holmes, H. Sanghera, Zamir K. Punja
Summary: Crown gall, caused by Agrobacterium tumefaciens, was found for the first time on cannabis plants in licensed production facilities in British Columbia, Canada. The incidence of the disease was extremely low, estimated at 0.01% of total plants.
CANADIAN JOURNAL OF PLANT PATHOLOGY
(2023)
Review
Biochemical Research Methods
Rishikesh Ghogare, Yvonne Ludwig, Gela Myan Bueno, Inez H. Slamet-Loedin, Amit Dhingra
Summary: Genome editing has the potential to rapidly improve crops to meet the challenges of feeding the planet in changing climates. Different delivery methods for editing reagents, particularly the use of RNPs, show high efficiency but face challenges. Innovative delivery approaches are being explored for routine non-transgenic genome editing in plants.
TRANSGENIC RESEARCH
(2021)
Article
Plant Sciences
Ye Tang, Zhennan Zhang, Zhiyuan Yang, Jiahe Wu
Summary: CRISPR/Cas9 and Agrobacterium tumefaciensvirulence proteins work together to achieve precise genome editing via homology directed repair.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Ecology
Roel Pel, Simon Dupin, Henk Schat, Jacintha Ellers, E. Toby Kiers, Nico M. van Straalen
EUROPEAN JOURNAL OF SOIL BIOLOGY
(2018)
Article
Multidisciplinary Sciences
Robin van Velzen, Rens Holmer, Fengjiao Bu, Luuk Rutten, Arjan van Zeijl, Wei Liu, Luca Santuari, Qingqin Cao, Trupti Sharma, Defeng Shen, Yuda Roswanjaya, Titis A. K. Wardhani, Maryam Seifi Kalhor, Joelle Jansen, Johan van den Hoogen, Berivan Gungor, Marijke Hartog, Jan Hontelez, Jan Verver, Wei-Cai Yang, Elio Schijlen, Rimi Repin, Menno Schilthuizen, M. Eric Schranz, Renze Heidstra, Kana Miyata, Elena Fedorova, Wouter Kohlen, Ton Bisseling, Sandra Smit, Rene Geurts
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Multidisciplinary Sciences
Maximilian Griesmann, Yue Chang, Xin Liu, Yue Song, Georg Haberer, Matthew B. Crook, Benjamin Billault-Penneteau, Dominique Lauressergues, Jean Keller, Leandro Imanishi, Yuda Purwana Roswanjaya, Wouter Kohlen, Petar Pujic, Kai Battenberg, Nicole Alloisio, Yuhu Liang, Henk Hilhorst, Marco G. Salgado, Valerie Hocher, Hassen Gherbi, Sergio Svistoonoff, Jeff J. Doyle, Shixu He, Yan Xu, Shanyun Xu, Jing Qu, Qiang Gao, Xiaodong Fang, Yuan Fu, Philippe Normand, Alison M. Berry, Luis G. Wall, Jean-Michel Ane, Katharina Pawlowski, Xun Xu, Huanming Yang, Manuel Spannagl, Klaus F. X. Mayer, Gane Ka-Shu Wong, Martin Parniske, Pierre-Marc Delaux, Shifeng Cheng
Article
Plant Sciences
Arjan van Zeijl, Titis A. K. Wardhani, Maryam Seifi Kalhor, Luuk Rutten, Fengjiao Bu, Marijke Hartog, Sidney Linders, Elena E. Fedorova, Ton Bisseling, Wouter Kohlen, Rene Geurts
FRONTIERS IN PLANT SCIENCE
(2018)
Article
Biochemistry & Molecular Biology
Matthew D. Whiteside, Gijsbert D. A. Werner, Victor E. A. Caldas, Anouk Van't Padje, Simon E. Dupin, Bram Elbers, Milenka Bakker, Gregory A. K. Wyatt, Malin Klein, Mark A. Hink, Marten Postma, Bapu Vaitla, Ronald Noe, Thomas S. Shimizu, Stuart A. West, E. Toby Kierst
Article
Plant Sciences
Fengjiao Bu, Luuk Rutten, Yuda Purwana Roswanjaya, Olga Kulikova, Marta Rodriguez-Franco, Thomas Ott, Ton Bisseling, Arjan van Zeijl, Rene Geurts
Article
Plant Sciences
Luuk Rutten, Kana Miyata, Yuda Purwana Roswanjaya, Rik Huisman, Fengjiao Bu, Marijke Hartog, Sidney Linders, Robin van Velzen, Arjan van Zeijl, Ton Bisseling, Wouter Kohlen, Rene Geurts
Article
Multidisciplinary Sciences
Jieshun Lin, Yuda Purwana Roswanjaya, Wouter Kohlen, Jens Stougaard, Dugald Reid
Summary: Legumes balance nitrate signaling and symbiotic nitrogen fixation through cytokinin biosynthesis, with low nitrate conditions promoting cytokinin induction and high nitrate inhibiting it. This differential response is further seen in mutants, which show hypersensitivity to nitrate inhibition but can be rescued by cytokinin application. Nitrate's inhibitory effect on symbiosis occurs in a Nlp1 and Nlp4 dependent manner, contrasting with its positive influence on non-legumes.
NATURE COMMUNICATIONS
(2021)