Article
Microbiology
Madjid Morsli, Christine Zandotti, Aurelie Morand, Philippe Colson, Michel Drancourt
Summary: In this case report, metagenomic next-generation sequencing successfully identified Echovirus 12 as an etiological agent of acute meningitis in pediatric population, highlighting the potential of this method in improving the accuracy of viral diagnosis.
Article
Infectious Diseases
Yukang Dong, Yingying He, Xia Zhou, Xia Lv, Jia Huang, Yaqi Li, Xin Qian, Fangfang Hu, Jiaying Zhu
Summary: This case report highlights the use of metagenomic next-generation sequencing (mNGS) in diagnosing Mycoplasma hominis meningitis, which is a rare postoperative complication of neurosurgery. mNGS showed high sensitivity and specificity in identifying the pathogen, enabling targeted clinical treatment. Combination with polymerase chain reaction (PCR) is recommended for early identification of pathogens and targeted medication.
INFECTION AND DRUG RESISTANCE
(2022)
Review
Immunology
Zheng-Bing Xiang, Er-Ling Leng, Wen-Feng Cao, Shi-Min Liu, Yong-Liang Zhou, Chao-Qun Luo, Fan Hu, An Wen
Summary: mNGS has good specificity and moderate sensitivity in diagnosing TBM. A more sensitive test should be developed to assist in the diagnosis of TBM.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Clinical Neurology
Aiqing Lin, Baotao Cheng, Xiaochun Han, Hong Zhang, Xiaoli Liu, Xueping Liu
Summary: The study aimed to evaluate the application of next-generation sequencing (NGS) technology in the early diagnosis of TBM patients. The results showed that NGS technology combined with MTB culture could increase the detection rate of TBM.
JOURNAL OF THE NEUROLOGICAL SCIENCES
(2021)
Article
Immunology
Zhouqing Gan, Jia Liu, Yijie Wang, Lu Yang, Zheng Lou, Han Xia, Min Li, Zhuolin Chen, Ying Jiang, Fuhua Peng
Summary: This study demonstrates that cerebrospinal fluid (CSF) metagenomic next-generation sequencing (mNGS) can be considered as a supplementary test for the diagnosis of cryptococcal meningitis (CM) and for directly distinguishing Cryptococcus gattii from Cryptococcus neoformans in clinical specimens.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Xiao-Wei Xing, Su-Fei Yu, Jia-Tang Zhang, Rui-Shu Tan, Yu-Bao Ma, Xia Tian, Rong-Fei Wang, Guo-En Yao, Fang Cui, Qiu-Ping Gui, Sheng-Yuan Yu
Summary: The study evaluated the utility of mNGS for the diagnosis of CA in CSF, with a sensitivity of 85.71% and specificity of 84.62%, which may reduce the need for cerebral biopsy in patients suspected of having CA.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Medicine, General & Internal
Yaoguang Li, Mengfan Jiao, Ying Liu, Zhigang Ren, Ang Li
Summary: Metagenomic next-generation sequencing (mNGS) is a hypothesis-free test that can detect Mycobacterium tuberculosis and other co-infection pathogens or underlying tumors simultaneously. However, compared with conventional assays, it is more expensive and may be overqualified for patients with mild and typical symptoms.
FRONTIERS IN MEDICINE
(2022)
Article
Medicine, General & Internal
Yong Liu, Xichao Mo, Ye Feng, Rodney E. Willoughby, Xing Weng, Yuyang Wang, Xing Li, Junling Gao, Jinfei Tian, Jie Peng
Summary: Rabies is a lethal disease for which an accurate diagnostic assay is highly desirable. This study found that metagenomic next-generation sequencing can successfully identify the rabies virus, making it a useful screening tool for diagnosing rabies, especially in cases without timely laboratory testing or exposure history.
FRONTIERS IN MEDICINE
(2023)
Article
Infectious Diseases
Xi Liu, Yuanli Chen, Hui Ouyang, Jian Liu, Xiaoqing Luo, Yayi Huang, Yan Chen, Jinmin Ma, Jinyu Xia, Li Ding
Summary: Combining mNGS with conventional detection methods can increase the detection rate for Mycobacterium tuberculosis complex (MTBC). Additionally, mNGS is able to identify new potential pathogens, aiding in better diagnosis of coinfection.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Review
Public, Environmental & Occupational Health
Yulian Li, Wentao Bian, Shiping Wu, Jie Zhang, Dan Li
Summary: This meta-analysis evaluated the performance of mNGS in diagnosing tuberculosis and found that it has high sensitivity and can rapidly diagnose Mycobacterium tuberculosis. Various factors, such as sample types, study types, and sample sizes, may contribute to heterogeneity. In a population with a 10% prevalence rate, the accuracy of sensitivity reached 94%.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Immunology
Ying Liu, Huifen Wang, Yaoguang Li, Zujiang Yu
Summary: The study aimed to evaluate the clinical diagnostic value of metagenomic next-generation sequencing (mNGS) for tuberculosis (TB). Retrospective analysis of 52 patients with suspected TB infection was conducted, and multiple detection methods were performed. The results showed that mNGS had a higher detection rate of MTB compared to acid-fast staining and PCR, but no statistical difference with T-SPOT.TB assay. As an adjunctive diagnostic technology, mNGS can be combined with traditional methods to guide the diagnosis and treatment of TB.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Min Kong, Wei Li, Qingsheng Kong, Haixin Dong, Aizhong Han, Liqing Jiang
Summary: This study describes a case of a child infected with tuberculosis after being wounded. The use of metagenomic next-generation sequencing (mNGS) helped in identifying the specific pathogen and facilitating early treatment, leading to improved prognosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Rong Zhang, Yan Zhuang, Zheng-Hui Xiao, Cai-Yun Li, Fan Zhang, Wei-Qing Huang, Min Zhang, Xiao-Ming Peng, Chao Liu
Summary: In this study, metagenomic next-generation sequencing (mNGS) was used for etiological diagnosis and monitoring antibiotic treatment efficacy in neonatal patients with severe infections. mNGS identified microbial findings in all cases, leading to changes in antibiotic treatment. This method has the potential to reduce the overall cost and burden of disease management in neonatal infections.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Medicine, General & Internal
Junyan Qu, Zhiyong Zong
Summary: This case report presents a case of strongyloidiasis in a Chinese male patient diagnosed using metagenomic next-generation sequencing (mNGS). The study highlights the potential of mNGS in detecting uncommon infectious diseases and emphasizes the importance of raising awareness of strongyloidiasis in non-endemic areas.
FRONTIERS IN MEDICINE
(2022)
Article
Infectious Diseases
Xiaobo Zhang, Chao Jiang, Chaojun Zhou
Summary: MNGS technology plays a crucial role in the diagnosis of E. faecalis meningitis, assisting in confirming the diagnosis and treatment plan even when CSF culture is negative.
BMC INFECTIOUS DISEASES
(2021)
Review
Biotechnology & Applied Microbiology
Chufeng Chen, Jiaying Liu, Yidong Chen, Anqi Lin, Weiming Mou, Lingxuan Zhu, Tao Yang, Quan Cheng, Jian Zhang, Peng Luo
Summary: Researches have shown that chronic viral infection and persistent exposure to antigens and/or inflammatory signals in cancer can lead to T cell exhaustion due to major changes in the epigenetic and metabolic environment. The discovery of the immune checkpoint pathway has been a significant milestone in understanding and reversing T cell exhaustion. Antibodies targeting these pathways have demonstrated superior capability in reversing T cell exhaustion. However, immune checkpoint blocking therapy still has limitations, such as short-term therapeutic effects and high individual heterogeneity. Assay for transposase-accessible chromatin with sequencing (ATAC-seq) is a method that can analyze the accessibility of whole-genome chromatin. Increasing studies have reported that ATAC-seq can characterize the dynamic changes of epigenetics in T cell exhaustion. It has been found that immune checkpoint blocking therapy can only temporarily restore the function of exhausted T cells due to irreversible changes in their epigenetics. In this study, we review the latest developments that provide a clearer molecular understanding of T cell exhaustion, reveal potential new therapeutic targets for persistent viral infection and cancer, and offer new insights for designing effective immunotherapy for cancer and chronic infection.
CANCER GENE THERAPY
(2023)
Article
Oncology
Zaoqu Liu, Hui Xu, Siyuan Weng, Chunguang Guo, Qin Dang, Yuyuan Zhang, Yuqing Ren, Long Liu, Libo Wang, Xiaoyong Ge, Zhe Xing, Jian Zhang, Peng Luo, Xinwei Han
Summary: A machine learning-based prognostic signature (MLPS) has been developed to predict the prognosis of melanoma patients, showing superior predictive performance compared to clinical traits and other collected signatures.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2023)
Article
Oncology
Tianqi Gu, Aimin Jiang, Chaozheng Zhou, Anqi Lin, Quan Cheng, Zaoqu Liu, Jian Zhang, Peng Luo
Summary: This study aims to determine the safety of immune checkpoint inhibitors combined with the anti-angiogenesis drug bevacizumab through a large-scale retrospective analysis of adverse drug reactions (ADRs). The risk of ADRs such as interstitial lung disease, hypertension, and gastrointestinal bleeding was found to be elevated in patients receiving combination therapy.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Plant Sciences
Xiaowen Zhao, Xiaomai Yuan, Yuanjun Xing, Jicao Dao, Deqiang Zhao, Yuze Li, Weiwei Li, Ziting Wang
Summary: This study conducted a meta-analysis of 57 studies on the effect of plant growth-promoting rhizobacteria (PGPR) inoculation on plants under drought conditions. The results showed that PGPR inoculation helps plants recover from drought stress and improves drought resistance, particularly for C4 plants. Furthermore, PGPR is more effective in increasing plant biomass, enhancing photosynthesis, and inhibiting oxidant damage under drought conditions. Different taxa and functional traits of PGPR also have varying degrees of effect on plant drought resistance.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Cell Biology
Zeyu Wang, Ziyu Dai, Hao Zhang, Nan Zhang, Xisong Liang, Luo Peng, Jian Zhang, Zaoqu Liu, Yun Peng, Quan Cheng, Zhixiong Liu
Summary: In this study, we collected 73 GBM samples and found that the different subtypes of GBM have different prognoses. We also discovered the association between pyroptosis and tumor-microglia interaction and the tumor response to interferon-gamma. Based on pyroptosis-related genes, we grouped GBM samples into cluster 1 and cluster 2, with cluster 1 samples showing worse prognosis and a more complex immune landscape. Machine learning algorithms identified nutlin-3 as a potential treatment for cluster 1 samples.
CELL PROLIFERATION
(2023)
Article
Chemistry, Physical
Fushao Li, Yingxian Xu, Qingqing Wu, Deqiang Zhao, Mingsen Deng
Summary: The fibrous La1.2Sr0.8CoO4 +/- 6 cathode material is prepared using soluble salts as metal-ion sources and polyacrylonitrile (PAN) as a polymer matrix via electrostatic spinning. The microstructural characteristic of this material is investigated, and its electrochemical performance in solid-oxide fuel cells is tested. The results demonstrate that the fibrous cathode exhibits superior electrochemical performance compared to the conventional plain cathode.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Oncology
Jiexia Zhang, Ningning Zhou, Huojin Deng, Xin Chen, Qunqing Chen, Qiongyao Wang, Lei Sun, Yang Wen, Xiaolong Cao, Zhiqiang Luo, Jian Zhang, Weiliang Zhu, Linlang Guo
Summary: This study used liquid biopsies to monitor and analyze plasma samples from patients with extensive-stage SCLC, and found that ctDNA levels can reflect tumor burden and predict progression-free survival. The study also demonstrated that plasma ctDNA assays can provide real-time information on somatic mutations for potential targeted therapies for SCLC.
Article
Chemistry, Inorganic & Nuclear
Zhimo Yang, Kun Liu, Yiwen Guo, Deqiang Zhao, Ning Zhang, Xiaodong Sun, Yuhao Zhou, Wenlong Liu, Juncai Sun
Summary: Metal doping is crucial for enhancing the electronic conductivity of Sn4P3. This study presents a facile synthesis of Fe-doped Sn4P3 through a solid-state reaction process. The Fe-doped Sn4P3 shows stacking of numerous nanoparticles, with Fe3+ easily doping into the Sn4P3 structure due to its slightly smaller ionic radius compared to Sn4+. The Sn4P3 anode with 5% Fe doping exhibits a higher initial discharge capacity of 1105.1 mAh/g and a columbic efficiency of 86.2%. After 200 cycles, a high discharge capacity of 972.4 mAh/g is achieved, while the undoped Sn4P3 anode only maintains at around 423.3 mAh/g. Fe doping acts as an inactive matrix, dispersing among Sn atoms to inhibit aggregation during cycling. The results demonstrate the vital role of Fe doping in enhancing the stability and electrochemical performance of the Sn4P3 architecture. This facile solid-state reaction process can be scaled up for the production of other metal-doped Sn4P3 in the field of lithium-ion batteries.
SOLID STATE SCIENCES
(2023)
Editorial Material
Immunology
Clare Y. Slaney, Peng Luo, Feng-Ming Kong, Linlang Guo, Jian Zhang
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Chong-Rui Xu, Qixun Chen, Chengzhi Zhou, Lin Wu, Wen Li, Huizhong Zhang, Yongsheng Li, Fei Xu, Jianping Xiong, Qiming Wang, Haibo Zhang, Yuequan Jiang, Haitao Yin, Qingchen Wu, Qiangsheng Dai, Jian Hu, Jianhua Chen, Jian Zhang, Gang Wu, Jun Yin, Jianfu Zhao, Baogang Liu, Jianzhen Shan, Liming Sheng, Qunqing Chen, Zhengxiang Han, Huaqiu Shi, Yimin Liu, Jun Chen, Yi-Long Wu
Summary: This study aimed to investigate the real-world effectiveness and safety of camrelizumab in inoperable or advanced non-small cell lung cancer (NSCLC) patients. The results showed that the objective response rate of camrelizumab was 40.2%, disease control rate was 85.1%, and 6-month progression-free survival and overall survival rates were 73.0% and 93.1%, respectively. The presence of liver metastasis and longer duration of treatment were associated with worse outcomes.
TRANSLATIONAL LUNG CANCER RESEARCH
(2023)
Article
Chemistry, Physical
Dandan Han, Bin Zhang, Deqiang Zhao, Jianwei Zhao, Yuhang Liu, Shunri Zheng, Li Fan, Changhao Wang, Dawei Wang, Fanling Meng
Summary: In this work, lead-free (1-x)Ba0.85Ca0.15Zr0.1Ti0.9O3-xBi(Mg2/3Ta1/3)O3 (BCZT-BMTx) relaxor ferroelectrics (RFEs) were prepared via a traditional solid-state reaction. The introduction of BMT inhibited grain growth and induced a phase transition from ferroelectric to relaxor ferroelectric. Ta2O5 doping significantly improved the breakdown strength (Eb) of BCZT ceramics. BCZT-BMT8 ceramic exhibited high energy efficiency (q) of 93.87% and recoverable energy density (Wrec) of 3.4 J/cm3 at a relatively high Eb of 440 kV/cm, making it a promising candidate for energy storage capacitors.
JOURNAL OF ALLOYS AND COMPOUNDS
(2023)
Review
Biochemistry & Molecular Biology
Zhifei Gao, Yifeng Bai, Anqi Lin, Aimin Jiang, Chaozheng Zhou, Quan Cheng, Zaoqu Liu, Xin Chen, Jian Zhang, Peng Luo
Summary: As a nontraditional T-cell subgroup, gamma delta T cells have shown great potential in immunotherapy for tumors. Immune checkpoint inhibitors (ICIs) have become pioneer drugs in tumor immunotherapy, and studies have shown that targeting ICs can reverse the dysfunctional state of gamma delta T cells in the tumor microenvironment and enhance their antitumor effects. Understanding the functional state of gamma delta T cells and their interaction with ICs will solidify ICIs combined with gamma delta T cells as a viable treatment option.
Article
Engineering, Chemical
Ling Yu, Hui Wang, Qi Huang, Hainan Liu, Qingkong Chen, Bojie Yuan, Qi Li, Xiaofei Zhao, Junwang Tang, Deqiang Zhao
Summary: A series of BiVO4/InVO4 composites were synthesized by regulating the ratio of Bi/In using a one-pot microwave method. The photocatalysts exhibited higher photocatalytic activities (100%) compared to pure InVO4 and BiVO4. Characterization techniques including X-ray diffraction, X-ray photoelectron spectroscopy, transmission electron microscopy, UV-vis diffuse reflection spectroscopy, and Fourier transform infrared spectroscopy confirmed the formation of BiVO4/InVO4 with enhanced solar light absorption. The improved reduction rate is attributed to efficient charge carrier separation due to the heterojunction formed between BiVO4 and InVO4. The potential photocatalytic mechanism of the BiVO4/InVO4 composite was also discussed in detail.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Xin Li, Dingdi Wang, Yuan Zhong, Feng Jiang, Deqiang Zhao, Siqi Sun, Po Lu, Min Lu, Zhenyu Wang, Zhennan Wu, Yanbo Gao, Yu Zhang, William W. W. Yu, Xue Bai
Summary: In this study, efficient blue-emitting Sb3+-doped Cs2NaInCl6 nanocrystals (NCs) were selected as the host, and rare-earth (RE) ions (Sm3+, Eu3+, Tb3+, and Dy3+) were incorporated into the host, achieving an excellent PLQY of 80.1%. Femtosecond transient absorption measurement revealed that RE ions served as activator ions and filled deep vacancy defects. These RE ions-doped halide double perovskite NCs exhibited applications in anti-counterfeiting, optical thermometry, and white-light-emitting diodes (WLEDs).
Article
Engineering, Chemical
Qi Huang, Zhuyan Zhao, Xuhuan Zhao, Qi Li, Hainan Liu, Heng Lu, Qiuhong Li, Mao Du, Yongjie Cao, Yang Wang, Xuan Xu, Niklas Hedin, Deqiang Zhao
Summary: This study investigated the use of low band gap photocatalysts for sterilizing ceramic tiles in toilets using natural light. The Ag/InVO4/BiOBr composite showed high sterilization efficacy and was not affected by anions such as Cl-, SO42-, and NO3-.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)