Article
Genetics & Heredity
Linxiang Feng, Zian Feng, Jie Hu, Jiahui Gao, Ang Li, Xiaodong He, Liu Liu, Zuojun Shen
Summary: In this study, high-throughput sequencing of miRNAs in plasma exosomes from lung adenocarcinoma patients and healthy individuals was performed. The results identified hsa-miR-4454 and hsa-miR-619-5p as potential biomarkers for the early diagnosis of lung adenocarcinoma.
FRONTIERS IN GENETICS
(2023)
Article
Oncology
Jing Wu, Zian Feng, Rui Wang, Ang Li, Hong Wang, Xiaodong He, Zuojun Shen
Summary: The aim of this study is to identify plasma exosomal miRNAs as noninvasive biomarkers for the early diagnosis of lung adenocarcinoma. Through next-generation sequencing and bioinformatics analysis, three candidate miRNAs have been identified, and further experimental validation suggests the potential use of these miRNAs for early diagnosis of lung adenocarcinoma.
Article
Veterinary Sciences
Aparna Hebbar, Rajeev Chandel, Payal Rani, Suneel Kumar Onteru, Dheer Singh
Summary: This study aimed to evaluate the potential of miRNAs as estrus biomarkers in buffalo, finding that certain miRNAs showed altered levels in different estrous cycle phases, while others were undetected in buffalo urine. Future studies should further explore the biomarker potential of miR-99a-5p in a larger cohort.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Cell Biology
Carlotta C. Damanti, Enrico Gaffo, Federica Lovisa, Anna Garbin, Piero Di Battista, Ilaria Gallingani, Anna Tosato, Marta Pillon, Elisa Carraro, Maurizio Mascarin, Caterina Elia, Alessandra Biffi, Stefania Bortoluzzi, Lara Mussolin
Summary: Plasma exosomal miRNAs are promising biomarkers for cancer diagnosis, and miR-486-5p and miR-26a-5p were identified as stable reference miRNAs for normalization using qRT-PCR, with miR-26a-5p specifically highlighted as a reliable reference for studies on hematological malignancies.
Article
Genetics & Heredity
Yuanxue Yang, Yun Zhang, Aiyu Wang, Ailing Duan, Chao Xue, Kaiyun Wang, Ming Zhao, Jianhua Zhang
Summary: This study investigated the resistance of Spodoptera frugiperda to insecticides and the regulatory roles of miRNAs. RNA-Seq analysis revealed that differentially expressed miRNAs play an important role in the insecticide resistance of S. frugiperda. Overexpression of specific miRNAs significantly increased the mortality of S. frugiperda to insecticides.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Li Wang, Mingxin Zhang, Jiansheng Wang, Jia Zhang
Summary: This study identified prognostic factors, accurate treatment approaches, and effective postoperative adjuvant therapy strategies for patients with mixed-type gastric adenocarcinoma through microRNA sequencing data analysis. The miR-18a-5p was found to be a potential target for treatment, with common signal pathways suggesting reliable molecular targets for diagnosis and clinical treatment of mixed-type GA.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Jiung-Pang Huang, Chih-Chun Chang, Chao-Yu Kuo, Kuang-Jing Huang, Etienne M. Sokal, Kuan-Hsing Chen, Li-Man Hung
Summary: EXO-miRNAs have the potential to be used as biomarkers for diabetic HFpEF. The study found that miR-30d-5p and miR-126a-5p were associated with HFpEF, and may be important for the development of diagnostic tools for diabetic HFpEF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Daniele Carvalho Calvano Mendes, Carlos Marino Cabral Calvano Filho, Natalia Garcia, Marcos Desiderio Ricci, Jose Maria Soares Junior, Katia Candido Carvalho, Edmund Chada Baracat
Summary: The expression levels of miR-96-5p and miR-182-5p have been found to be upregulated in breast cancer, while the protein expression of FOXO3A is decreased. This phenomenon is more prominent in triple-negative breast cancer, suggesting the important role of these molecules in the biology and clinical behavior of this tumor type. These findings provide new perspectives for alternative target therapy options for breast cancer patients expressing both FOXO3A and its regulatory miRNAs.
Article
Microbiology
Ata Garajei, Milad Parvin, Hady Mohammadi, Abdolamir Allameh, Azin Hamidavi, Masoud Sadeghi, Azadeh Emami, Serge Brand
Summary: This study evaluated the expression of miR-486-3p, miR-561-5p, miR-548-3p, and miR-509-5p in tissue biopsy samples with and without OSCC. The results showed that the expression of miR-486-3p and miR-561-5p was significantly lower in cancer samples compared to normal tissue samples, while miR-548-3p expression increased in the OSCC group compared to the control group. There was no significant difference in miR-509-5p expression between OSCC and control groups.
Article
Medicine, General & Internal
Rafael Alfaro, Isabel Legaz, Victor Jimenez-Coll, Jaouad El Kaaoui El Band, Helios Martinez-Banaclocha, Jose Antonio Galian, Antonio Parrado, Anna Mrowiec, Carmen Botella, Maria Rosa Moya-Quiles, Francisco Boix, Jesus de la Pena-moral, Alfredo Minguela, Santiago Llorente, Manuel Muro
Summary: The study analyzed miRNAs involved in the differentiation and activation of B and T lymphocytes from kidney transplant patients' peripheral blood leukocytes for biomarkers of acute renal rejection. The results showed an increase in miR-191-5p, miR-223-3p, miR-346, miR-423-5p, miR-574-3p, and miR-181d in the acute rejection group, and an increase in miR-150-5p in the non-acute rejection group.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Oncology
Giovanni Cochetti, Luigi Cari, Vincenza Maula, Rosy Cagnani, Alessio Paladini, Michele Del Zingaro, Giuseppe Nocentini, Ettore Mearini
Summary: The early diagnosis of ccRCC is crucial for improving patients' survival. A previously developed algorithm based on the level of specific small RNAs in urine showed promising results in predicting the presence of kidney cancer. This study further validated the algorithm's effectiveness and demonstrated its potential application in noninvasive early diagnosis of ccRCC.
Article
Cell Biology
Rui Mao, Kehao Liu, Nana Zhao, Pengsen Guo, Yingxin Wu, Zheng Wang, Yanjun Liu, Tongtong Zhang
Summary: The study developed a reliable and personalized prognostic signature based on immune genes for gastric adenocarcinoma, which accurately predicts prognosis and has predictive value in different clinical subgroups. High-risk patients were characterized by low immune scores, distinctive immune cell proportions, different immune checkpoint profiles, and a low tumor mutational burden.
Article
Obstetrics & Gynecology
Delnya Gholami, Farzane Amirmahani, Reza Salman Yazdi, Tahereh Hasheminia, Hossein Teimori
Summary: The study found that up-regulation of miR-182-5p, miR-192-5p, and miR-493-5p may serve as potential biomarkers for TZ. Additionally, the expression level of the CRISP3 glycosylated isoform was significantly lower in TZ patients compared to those with normal sperm. These results suggest a significant role of CRISP3 protein and miRs in male infertility.
REPRODUCTIVE SCIENCES
(2021)
Article
Oncology
Qinggui Chen, Li Lin, Boliang Xiong, Wensheng Yang, Junli Huang, Huibo Shi, Zhenfa Wang
Summary: MiR-873-5p is associated with gastric cancer characteristics and can impact gastric cancer cell behavior and chemoresistance by targeting THUMPD1.
JOURNAL OF GASTROINTESTINAL ONCOLOGY
(2021)
Article
Genetics & Heredity
Liping Zou, Qiwen Feng, Wei Xia, Changhong Zhu
Summary: This study aimed to identify common targets of miR-223-3p, miR-122-5p, and miR-93-5p in PCOS and assess their transcript levels in PCOS rat ovaries. Transcriptome profiles of granulosa cells from PCOS patients were analyzed to identify DEGs, followed by miRNA target prediction and functional analysis. The study identified several potential genes, including JMJD1C, PLCG2, SMAD3, FOSL2, TGFB1, TRIB1, GAS7, TRIM25, NFYA, and CALCRL, which may play a role in the development of PCOS. These findings contribute to the identification of potential biomarkers for the prevention and treatment of PCOS in the future.
FRONTIERS IN GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Negin Raei, Reza Safaralizadeh, Mohammadali Hosseinpourfeizi, Saeid Latifi-Navid, Abbas Yazdanbod
Summary: This study found that NORAD is significantly overexpressed in gastric cancer tissues, and may promote tumor development by affecting cell migration, metastasis, apoptosis rate, and relevant protein levels. NORAD may serve as a potential diagnostic biomarker in gastric cancer.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Esmaeil Darvish Aminabad, Ahmad Mobed, Mohammad Hasanzadeh, Mohammad Ali Hosseinpour Feizi, Reza Safaralizadeh, Farzad Seidi
Summary: This study developed an electrochemical immunosensor based on gold nanoparticle-modified graphene for the ultra-sensitive monitoring of Parkinson's disease-related protein. The combination of gold nanoparticles and graphene enhanced the electrochemical activity of the materials, improving sensitivity and lowering the detection limit. This diagnostic method has potential applications in early-stage diagnosis and high-throughput screening of Parkinson's disease.
Correction
Chemistry, Multidisciplinary
Esmaeil Darvish Aminabad, Ahmad Mobed, Mohammad Hasanzadeh, Mohammad Ali Hosseinpour Feizi, Reza Safaralizadeh, Farzad Seidi
Summary: This study presents a modification of an immunosensing method using gold nanoparticles conjugated with graphene for sensitive detection of α-synuclein protein in human plasma samples. The method shows potential for early identification of Parkinson's disease and can be used for point-of-care analysis.
Article
Oncology
Mina Seifi Inallou, Reza Safaralizadeh, Ali Rajabi, Mohammadali Hosseinpourfeizi, Mehdi Haghi
Summary: This study investigated the association of SDMGC and TRIM16 with GC susceptibility and clinicopathological characteristics of GC patients. The results showed an increase in the expression of SDMGC and a decline in the expression pattern of TRIM16 among the Iranian population. SDMGC was found to have a tumor-accelerative function, while TRIM16 played a pivotal tumor-suppressing role in GC patients.
JOURNAL OF GASTROINTESTINAL CANCER
(2023)
Article
Biochemistry & Molecular Biology
Narges Dastmalchi, Reza Safaralizadeh, Seyed Mahdi Banan Khojasteh, Mahdi Abdoli Shadbad, Mohammad Ali Hosseinpourfeizi, Shirin Azarbarzin, Ali Rajabi, Behzad Baradaran
Summary: The combined restoration of miR-424-5p and miR-142-3p has been shown to be more effective in inhibiting MCF-7 breast cancer development by upregulating Bax and Beclin-1 and downregulating Bcl-2 and c-Myc, leading to cell cycle arrest and suppression of clonogenicity.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Sama Akbarzadeh, Samaneh Tayefeh-Gholami, Parisa Najari, Ali Rajabi, Tooraj Ghasemzadeh, Mohammadali Hosseinpour Feizi, Reza Safaralizadeh
Summary: This study aimed to evaluate the expression levels of HAR1A and HAR1B lncRNAs in the blood samples of MS patients and investigate their relationship with disease activity. The results showed a significant decrease in HAR1A expression and a moderate increase in HAR1B expression in SPMS patients. The expression levels of HAR1A and HAR1B were positively correlated in MS patients. The study suggests that HAR1A can be introduced as a novel biomarker for MS diagnosis.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Narges Dastmalchi, Sama Akbarzadeh, Farnaz Amini, Ali Rajabi, Reza Safaralizadeh
Summary: This study assessed the expression changes of APOC1P1-3 lncRNA in cancerous and adjacent non-tumorous tissues from cervical cancer patients. APOC1P1-3 expression was significantly increased in cancerous tissues and showed associations with lymph node involvement, tumor invasion depth, squamous type of cervical cancer, and age. APOC1P1-3 may serve as a potential biomarker for the diagnosis and treatment of cervical cancer patients.
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
(2023)
Article
Oncology
Elaheh Mohammadali, Reza Safaralizadeh, Arash Poursheikhani, Tooraj Asvadi, Behzad Baradaran, Shahram Teimourian
Summary: This study aimed to evaluate the expression of lncRNA TMPO-AS1 in Iranian patients with gastric cancer (GC). Results showed that TMPO-AS1 was overexpressed in cancerous tissues and could serve as a biomarker to distinguish tumor tissues from normal ones. However, further research is needed to confirm its potential application in the diagnosis, prognosis, and treatment of GC.
INTERNATIONAL JOURNAL OF CANCER MANAGEMENT
(2023)
Review
Pathology
Negin Raei, Reza Safaralizadeh, Saeid Latifi-Navid
Summary: Metastatic cancer ctDNA has a high frequency of mutations in TP53, HER-2, KRAS, and EGFR genes, and studying ctDNA can help identify patients with pre-malignant tumors and determine prognosis and appropriate treatments. However, obstacles to ctDNA detection and analysis should be addressed and more clinical trials are needed to study the significance of ctDNA in commonly diagnosed malignancies.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2023)
Article
Biochemistry & Molecular Biology
Mina Rasoolnezhad, Reza Safaralizadeh, Mohammad Ali Hosseinpour Feizi, Seyed Mahdi Banan-Khojasteh, Elmira Roshani Asl, Parisa Lotfinejad, Behzad Baradaran
Summary: Restoring miR-138-5p in combination with paclitaxel can suppress breast cancer cell migration, sustain colony formation and proliferation, enhance chemosensitivity to paclitaxel by regulating apoptosis factors, and arrest cells in specific phases of the cell cycle, representing a novel approach in breast cancer treatment.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Pathology
Hesam Moazzen, Ali Rajabi, Reza Safaralizadeh
Summary: This study investigated the expression differences of BOK-AS1, FAM215A, and FEZF1-AS1 genes between tumor and non-tumor tissues in gastric cancer patients. The results showed that these genes exhibited significantly increased expression in tumor tissues and may function as oncogenic factors. They could also serve as potential biomarkers for the diagnosis and treatment of gastric cancer.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Pathology
Hossein Akhgari, Neda Shokri, Parisa Dehghanzadeh, Samaneh Tayefeh-Gholami, Ali Rajabi, Reza Safaralizadeh
Summary: The study found that PCAT1, PCAT2, and PCAT5 were significantly overexpressed in gastric tumor tissues compared to non-cancerous tissues. Furthermore, these lncRNAs were suggested to be novel oncogenes involved in promoting and developing tumor cells in gastric cancer patients.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Review
Pathology
Elmira Aboutalebi Vand Beilankouhi, Mir Amirhossein Sajadi, Iraj Alipourfard, Peyman Hassani, Mohammad Valilo, Reza Safaralizadeh
Summary: When misfolded or unfolded proteins accumulate in the endoplasmic reticulum (ER) due to stress, the unfolded protein response (UPR) is activated. This process is disrupted in various diseases including diabetes, neurodegenerative diseases, and cancers. The UPR activates specific signaling pathways in the ER, nucleus, and cytoplasm to restore cellular homeostasis, either promoting cell survival or apoptosis. Disturbance in this balance can lead to cancer.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Medicine, General & Internal
Mojtaba Varshochi, Reyhaneh Ravanbakhsh Gavgani, Fatemeh Ravanbakhsh Ghavghani, Sina Hamzehzadeh
Summary: This report highlights the unexpected and rare complications that can occur in brucellosis, and emphasizes that these complications can be treated with standard treatment.
CLINICAL CASE REPORTS
(2023)
Article
Environmental Sciences
Reyhaneh Ravanbakhsh, Naser Agh, Mojtaba Nouraein, Peter Bossier
Summary: The ecological crisis in recent years has led to changes in the adaptation mechanisms of Artemia urmiana in Urmia Lake, with increased Hsp-70 expression levels and improved response to environmental stressors in nauplii exposed to long-term ecological crisis.
ENVIRONMENTAL RESEARCH
(2023)
