Article
Agriculture, Dairy & Animal Science
Justin T. H. Chow, Aaron R. Gall, Alexander K. Johnson, TuAnh N. Huynh
Summary: This study found a high incidence of L. monocytogenes shedding in lactating dairy cows in a Wisconsin farm, with isolates showing antibiotic resistance and the capability to infect human and murine cells. Differences in fecal bacterial communities were observed between shedding and nonshedding cows, indicating the potential negative influence of subclinical L. monocytogenes carriage on animal gut health.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Infectious Diseases
Pavel A. Andriyanov, Pavel A. Zhurilov, Elena A. Liskova, Tatyana I. Karpova, Elena V. Sokolova, Yulia K. Yushina, Elena V. Zaiko, Dagmara S. Bataeva, Olga L. Voronina, Ekaterina K. Psareva, Igor S. Tartakovsky, Denis V. Kolbasov, Svetlana A. Ermolaeva
Summary: Susceptibility testing of 117 L. monocytogenes strains to 23 antibiotics revealed a varied response, with most strains showing sensitivity to multiple antibiotics but some strains exhibiting resistance to specific drugs. There was a shift in the proportion of resistant strains over time, with different antibiotics showing unique patterns of resistance evolution.
Article
Biotechnology & Applied Microbiology
Cristina Rodriguez-Melcon, Victor Serrano-Galan, Rosa Capita, Carlos Alonso-Calleja
Summary: The aim of this research was to investigate the effect of subinhibitory concentrations of disinfectants on the resistance of Listeria monocytogenes to tetracycline. The results showed that exposure to subinhibitory doses of the disinfectants could lead to increased resistance to tetracycline. The use of disinfectants at subinhibitory concentrations should be avoided to prevent the development of resistance.
Article
Food Science & Technology
Cristina Rodriguez-Melcon, Carlos Alonso-Calleja, Rosa Capita
Summary: The study investigated the effects of low doses of four biocides on antibiotic resistance and cell surface hydrophobicity of Listeria monocytogenes and Salmonella enterica. The results showed that the use of subinhibitory concentrations of biocides could increase bacterial resistance to antibiotics and modify the hydrophobicity of the cell surface.
Article
Food Science & Technology
Cristina Rodriguez-Melcon, Alexandra Esteves, Javier Carballo, Carlos Alonso-Calleja, Rosa Capita
Summary: The impact of treating minced chicken meat with sodium nitrite, nisin, and lactic acid on microbial groups and antibiotic resistance of Listeria monocytogenes was investigated. The results showed that lactic acid treatment reduced microbial load, while the prevalence of L. monocytogenes did not differ significantly among the different treatments. All isolates showed resistance to multiple antibiotics, with higher resistance in the treated samples.
Article
Food Science & Technology
Adeoye John Kayode, Anthony Ifeanyi Okoh
Summary: We investigated the prevalence, genetic diversity, and antibiogram profiles of Listeria monocytogenes (Lm) in fruits and vegetables sourced from three District Municipalities in the Eastern Cape Province, South Africa. Lm was detected in a significant proportion of the samples tested, with high genetic diversity and multidrug resistance. This highlights the potential threat to consumers and the importance of food safety measures.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Food Science & Technology
Barbara Szymczak
Summary: An increasing number of atypical Listeria monocytogenes (LM) strains are being isolated from food, causing challenges in classification and testing. These atypical strains have distinct phenotypic and genotypic characteristics, and may pose a risk to human health.
Review
Food Science & Technology
Haoqi Zhang, Xin Luo, Zafeiro Aspridou, Ourania Misiou, Pengcheng Dong, Yimin Zhang
Summary: A meta-analysis comparing the prevalence and antibiotic resistance rate of Listeria monocytogenes in livestock and poultry meat between China and the European Union (EU) was conducted. The prevalence of L. monocytogenes in meat was 7.1% in China and 8.3% in Europe, with a decreasing trend observed in both regions. The pooled prevalence of antibiotic resistance was 5.8%, with the highest resistance found in oxacillin, ceftriaxone, and tetracycline. There was a significant difference between China and the EU in ceftriaxone (52.6% vs. 17.3%) and cefotaxime (7.0% vs. 0.0%). Enforcing good control measures against meat-sourced L. monocytogenes remains a significant challenge in both China and the EU.
Article
Immunology
Binghuai Lu, Junwen Yang, Chunyan Gao, Dong Li, Yanchao Cui, Lei Huang, Xingchun Chen, Duochun Wang, Aiping Wang, Yulei Liu, Yi Li, Zhijun Zhang, Mingyuan Jiao, Heping Xu, Yu Song, Baoqing Fu, Lili Xu, Qing Yang, Yongzhong Ning, Lijun Wang, Chunmei Bao, Guolan Luo, Hua Wu, Tongshu Yang, Chen Li, Manjuan Tang, Junrui Wang, Wenchen Guo, Ji Zeng, Wen Zhong
Summary: This study investigated the demographic and clinical features, outcomes, antibiotic susceptibility profiles, and molecular characteristics of L. monocytogenes causing listeriosis in China from 2008 to 2019. It identified a high genotypic diversity of L. monocytogenes strains and highlighted a high prevalence of ST87 and ST1 in listeriosis cases in China.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Hasan Tukenmez, Pardeep Singh, Souvik Sarkar, Melike Cakir, Ana H. Oliveira, Cecilia Lindgren, Karolis Vaitkevicius, Mari Bonde, A. Elisabeth Sauer-Eriksson, Fredrik Almqvist, Jorgen Johansson
Summary: Bacteria resistant to antibiotics are a major problem, requiring the development of new antimicrobial drugs. In this study, the effectiveness of a new compound, PS900, in inhibiting virulence factor expression in Listeria monocytogenes was demonstrated. Furthermore, PS900 was found to potentiate the bactericidal effects of cholic acid and osmolytes, making it a promising candidate for future antibacterial drug design.
Article
Biotechnology & Applied Microbiology
Phillip Brown, Kevin Hernandez, Cameron Parsons, Yi Chen, Nicholas Gould, Christopher S. Deperno, Jeffrey Niedermeyer, Sophia Kathariou
Summary: Listeria monocytogenes is a foodborne pathogen that can cause severe disease. This study analyzed strains of L. monocytogenes and non-pathogenic Listeria spp. isolated from wild black bears for antimicrobial resistance (AMR). The results showed that tetracycline resistance was more common in non-pathogenic Listeria strains and identified different resistance elements in these strains. The study suggests that non-pathogenic Listeria strains in wildlife may serve as reservoirs for AMR determinants and should be further monitored.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Food Science & Technology
Qifan Sun, Shuzhen Cai, Jianheng Cheng, Ying Zhang, Ruoqin Lin, Qinghua Ye, Liang Xue, Haiyan Zeng, Tao Lei, Shuhong Zhang, Xueting Luo, Kegang Wu, Qingping Wu, Moutong Chen, Jumei Zhang
Summary: Listeria monocytogenes was found to be present in 27.0% of samples collected in a mushroom plant, with specific strains persisting throughout different stages of production. The study suggests that critical disinfection measures are necessary to eliminate the persistence of L. monocytogenes in the facility.
Article
Food Science & Technology
Sarah Panera-Martinez, Cristina Rodriguez-Melcon, Victor Serrano-Galan, Carlos Alonso-Calleja, Rosa Capita
Summary: A study in North-Western Spain found that 73 out of 100 samples of fresh poultry preparations were contaminated with Listeria spp., with Listeria monocytogenes being the most common species. These bacteria showed resistance to multiple antimicrobials, including antibiotics commonly used to treat human listeriosis. This highlights the need to handle poultry preparations correctly to prevent the spread of drug-resistant Listeria strains.
Article
Microbiology
Robert Hanes, Fangyuan Zhang, Zuyi Huang
Summary: Listeria monocytogenes is a dangerous foodborne pathogen that poses a significant threat to the food industry. A data analysis approach was used to study its stress response, virulence, and antimicrobial resistance by creating protein interaction networks. Key proteins were identified, including sigB, flaA, cheA, cheY, and lmo0693, which show high potential as targets for combating L. monocytogenes. These findings offer new directions for improving food preservation methods and developing treatments for L. monocytogenes.
Article
Microbiology
Rubina Paradiso, Marita Georgia Riccardi, Bianca Cecere, Nunzia Riccone, Roberto Scialla, Antonietta Anzalone, Anna Cerrone, Esterina De Carlo, Giorgia Borriello, Giovanna Fusco
Summary: Listeria monocytogenes is a Gram-positive pathogen that can cause life-threatening infections. Whole genome sequencing is a powerful method for molecular typing of this pathogen and can provide insights into its pathogenic potential.
Article
Infectious Diseases
Raskit Lachmann, Sven Halbedel, Marlen Adler, Natalie Becker, Franz Allerberger, Alexandra Holzer, Idesbald Boone, Gerhard Falkenhorst, Sylvia Kleta, Sascha Al Dahouk, Klaus Stark, Petra Luber, Antje Flieger, Hendrik Wilking
Summary: This study identified a nationwide outbreak of listeriosis in Germany through whole genome sequencing and molecular typing, tracing the source to a meat products company. The outbreak was controlled through product recall and company closure, leading to no further cases being detected.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Microbiology
Jeanine Rismondo, Lisa M. Schulz, Maria Yacoub, Ashima Wadhawan, Michael Hoppert, Marc S. Dionne, Angelika Gruendling
Summary: The ABC transporter EslABC is associated with the intrinsic lysozyme resistance of Listeria monocytogenes. The deletion of EslB not only affects lysozyme resistance, but also influences endogenous cell lysis, cell wall biosynthesis, cell division, and growth in media containing high concentrations of sugars. EslB is an important determinant for cell wall integrity in L. monocytogenes through a yet-unknown mechanism.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Microbiology
Samuel Hauf, Tim Engelgeh, Sven Halbedel
Summary: Listeria monocytogenes, an environmental bacterium, has been found to develop resistance against the antibiotic aurantimycin in soil, thanks to the genes lieAB and lftRS. The tight repression and induction of these genes play a crucial role in survival in natural habitats. The study sheds light on the molecular mechanisms behind aurantimycin resistance in L. monocytogenes.
JOURNAL OF BACTERIOLOGY
(2021)
Review
Microbiology
Jeanine Rismondo, Annika Gillis, Angelika Grundling
Summary: This article provides a brief overview of the functions and complexity of secondary cell wall polymers in Gram-positive bacteria, focusing on the biosynthesis pathways of teichoic acid and complex polysaccharides, as well as the recent glycosylation mechanism.
CURRENT OPINION IN MICROBIOLOGY
(2021)
Article
Microbiology
Sabrina Wamp, Patricia Rothe, Daniel Stern, Gudrun Holland, Janina Doehling, Sven Halbedel
Summary: This study discovered a new signaling route that controls PG production in the human pathogen Listeria monocytogenes and its role in intrinsic resistance to beta-lactam antibiotics. This signaling involves the PrkA kinase and its substrate ReoM, which regulates PG biosynthesis by controlling the stability of MurA. The transglycosylase RodA and the transpeptidase PBP B3 are also important downstream factors. These findings shed new light on the regulation of PG biosynthesis and antibiotic resistance in Gram-positive bacteria.
Article
Immunology
Raskit Lachmann, Sven Halbedel, Stefanie Lueth, Alexandra Holzer, Marlen Adler, Ariane Pietzka, Sascha Al Dahouk, Klaus Stark, Antje Flieger, Sylvia Kleta, Hendrik Wilking
Summary: Invasive listeriosis outbreaks associated with the consumption of smoked and graved salmon products were investigated in Germany. Whole-genome sequencing and patient interviews were used to identify and analyze the outbreaks. The study identified 22 independent outbreaks between 2010 and 2021, with 228 cases and 50 deaths in Germany. Cross-border outbreaks were also observed. The findings highlight the serious risk posed by L. monocytogenes-contaminated salmon products in Germany, emphasizing the need for improved hygiene measures and better awareness among susceptible individuals.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Microbiology
Mengyi Wang, Sabrina Wamp, Johannes Gibhardt, Gudrun Holland, Inge Schwedt, Kai-Uwe Schmidtke, Katrin Scheibner, Sven Halbedel, Fabian M. Commichau
Summary: This study investigates the phenotypes of mutants with defects in c-di-AMP metabolism in Listeria monocytogenes and identifies suppressor mutants that restore their growth defects. The results show that c-di-AMP plays a key role in controlling osmolyte homeostasis in L. monocytogenes, and that the Opp oligopeptide transport system is involved in the uptake of the antibiotic fosfomycin. These findings contribute to our understanding of the complex metabolic regulation in L. monocytogenes.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Martin A. Fischer, Tim Engelgeh, Patricia Rothe, Stephan Fuchs, Andrea Thuermer, Sven Halbedel
Summary: Listeria monocytogenes, a Gram-positive bacterium, is widespread in the environment and infects humans via contaminated food, posing a particularly dangerous threat to at-risk patients. By using Tn-Seq technology, crucial cellular processes and genes necessary for pathogen growth both inside and outside the host have been identified, offering potential novel options for developing antilisterial drugs.
Article
Chemistry, Medicinal
Ann-Britt Schafer, Maurice Steenhuis, Kin Ki Jim, Jolanda Neef, Sarah O'Keefe, Roger C. Whitehead, Eileithyia Swanton, Biwen Wang, Sven Halbedel, Stephen High, Jan Maarten van Dijl, Joen Luirink, Michaela Wenzel
Summary: Eeyarestatin 24 (ES24) is a promising new antibiotic that inhibits SecYEG-mediated protein transport in Gram-negative bacteria and has notable activity against Gram-positive bacteria. It causes DNA damage and membrane potential dissipation in Escherichia coli but not in Bacillus subtilis. ES24 differs from nitrofurantoin in its mechanism of action and outperforms it in a zebrafish embryo pneumococcal infection model, suggesting multiple targets in bacteria.
ACS INFECTIOUS DISEASES
(2023)
Article
Microbiology
Sven Halbedel, Ida Sperle, Raskit Lachmann, Sylvia Kleta, Martin A. Fischer, Sabrina Wamp, Alexandra Holzer, Stefanie Lueth, Larissa Murr, Christin Freitag, Laura Espenhain, Roger Stephan, Ariane Pietzka, Susanne Schjorring, Guido Bloemberg, Mareike Wenning, Sascha Al Dahouk, Hendrik Wilking, Antje Flieger
Summary: The use of whole-genome sequencing (WGS) has allowed for the identification of a multinational listeriosis outbreak caused by Listeria monocytogenes. The outbreak was traced back to smoked rainbow trout filets from a Danish producer, highlighting the potential for international food trade to contribute to the spread of infectious diseases. This study emphasizes the importance of surveillance and collaboration in preventing further outbreaks of listeriosis.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Jeanine Rismondo, Cornelia Grosse, Dietrich H. Nies
Summary: The combined actions of an inner membrane with a transenvelope efflux system play an important role in the efficient removal of essential-but-toxic transition metal cations in bacteria.
MICROBIOLOGY SPECTRUM
(2023)
Article
Environmental Sciences
Lisa Maria Schulz, Alicia Konrath, Jeanine Rismondo
Summary: Listeria monocytogenes is a foodborne pathogen that can grow in various environmental conditions. In this study, two wildtype strains of L. monocytogenes were examined for their ability to grow under salt and pH stress in Listeria synthetic medium (LSM). It was found that a wide range of carbon sources could support the growth of these strains in LSM, but hexose phosphate sugars required pre-growth under conditions where the virulence regulator PrfA was active. Additionally, the amino acid sugar N-acetylmannosamine (ManNAc) promoted the growth of both strains in LSM, with specific genes in the lmo2795-nanE operon playing a role in this process.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2023)
Article
Microbiology
Martin A. Fischer, Andrea Thuermer, Antje Flieger, Sven Halbedel
Summary: This study presents the closed genome sequences of three clinical Listeria monocytogenes strains of MLST sequence type 8 (ST8), which were associated with three separate listeriosis outbreak clusters in Germany between 2012 and 2020.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Biochemistry & Molecular Biology
Chih-Hung Wu, Jeanine Rismondo, Rhodri M. L. Morgan, Yang Shen, Martin J. Loessner, Gerald Larrouy-Maumus, Paul S. Freemont, Angelika Grundling
Summary: Research showed that Bacillus subtilis has multiple UDP-glucose synthesizing enzymes, among which YngB is a newly discovered enzyme with UGPase activity. YngB can reintroduce glucose residues on wall teichoic acid and produce glucolipids in a B. subtilis gtaB mutant.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)