Article
Biochemistry & Molecular Biology
Maciej Jaskiewicz, Adrianna Moszynska, Jaroslaw Kroliczewski, Aleksandra Cabaj, Sylwia Bartoszewska, Agata Charzynska, Magda Gebert, Michal Dabrowski, James F. Collawn, Rafal Bartoszewski
Summary: HIF is a transcription factor that activates the adaptive hypoxic response during low oxygen levels. HIF-1 and HIF-2 mediate this response in a sequential manner, but their transcriptional profiles differ and the transition between them is not well understood.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2022)
Article
Agriculture, Dairy & Animal Science
Yang Yu, Jian He, Wenhui Liu, Zhimin Li, Shaoping Weng, Jianguo He, Changjun Guo
Summary: This study investigated the characterization of PHD2 gene from mandarin fish and its role in the HIF-1 signaling pathway. The results showed that overexpression of scPHD2 could inhibit the HIF-1 signaling pathway and promote the degradation of scHIF-1a. This study enhances our understanding of the molecular mechanisms underlying hypoxia adaptation in teleost fish.
Review
Biochemistry & Molecular Biology
Thuy-Hang Nguyen, Stephanie Conotte, Alexandra Belayew, Anne-Emilie Decleves, Alexandre Legrand, Alexandra Tassin
Summary: Muscular dystrophies are genetic degenerative muscle disorders characterized by muscle wasting, often accompanied by respiratory impairments and hypoxemia. Hypoxic stress activates compensatory mechanisms, with HIF-1α playing a key role. Alterations in HIF-1α in muscle may impact the pathophysiology of muscular dystrophies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Ju-Won Jang, Sojin Park, Eun-Yi Moon
Summary: B cells may be regulated by crosstalk between HIF-1 alpha and Nrf2 through ROS-mediated Syk activation, where HIF-1 alpha levels might control Nrf2 levels and vice versa, possibly associated with Syk phosphorylation.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yu-Kun Jie, Jian-Jun Jiang, Hong-Ling Ma, Chang -Hong Cheng, Guang-Xin Liu, Si-Gang Fan, Juan Feng, Zhi-Xun Guo
Summary: In this study, a novel PHD2 gene, SpPHD2, was cloned and identified in the mud crab Scylla paramamosain. The mRNA expression of SpPHD2 was widely distributed across all examined tissues, and the protein was mainly localized in the cytoplasm. Silencing SpPHD2 in vivo upregulated SpHIF-1 alpha and downstream genes, while overexpression of SpPHD2 in vitro dose-dependently reduced SpHIF-1 alpha transcriptional activity, indicating the crucial role of SpPHD2 in regulating SpHIF-1 alpha.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Oncology
Leah Davis, Matthias Recktenwald, Evan Hutt, Schuyler Fuller, Madison Briggs, Arnav Goel, Nichole Daringer
Summary: Hypoxia, or inadequate oxygen supply, is a characteristic of the tumor microenvironment and is associated with a poor prognosis and resistance to therapy. HIF-alpha, specifically the HIF-1 alpha and HIF-2 alpha subunits, play a crucial role in mediating the transcription of important proteins involved in tumor progression. While HIF-1 alpha has been extensively studied, the role of HIF-2 alpha is often overlooked. HIF-2 alpha can enhance the aggressive behavior of tumors, promoting metastasis, tissue remodeling, angiogenesis, and the upregulation of cancer stem cell factors. This review highlights the structure, function, hypoxic response, and roles of HIF-2 alpha in cancer progression, as well as discussing current therapeutic strategies and future directions.
Review
Cell Biology
Minghui Zhang, Yu Zhang, Yubo Ding, Jialu Huang, Jingwei Yao, Zhuoyi Xie, Yufan Lv, Jianhong Zuo
Summary: This review examines the regulatory roles of HIF-1 alpha and lncRNAs in the EMT process of tumor cells and their relationship with tumorigenesis, and summarizes current drugs that inhibit tumor progression by modulating HIF-1 alpha.
Article
Biochemistry & Molecular Biology
Kenta Kaihara, Shuji Nakagawa, Yuji Arai, Hiroaki Inoue, Shinji Tsuchida, Yuta Fujii, Yoichiro Kamada, Tsunao Kishida, Osam Mazda, Kenji Takahashi
Summary: The study found that hypoxia can suppress the development of rheumatoid arthritis by controlling HIF-1 alpha expression, delaying joint swelling and reducing joint destruction. Deferoxamine-mediated activation of HIF-1 alpha can counteract the inhibitory effect of sustained hypoxia on pro-inflammatory cytokines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Nasim Kheshtchin, Jamshid Hadjati
Summary: Hypoxia, a common characteristic of solid tumors, contributes to different aspects of tumor progression and limits the efficacy of immunotherapies. Developing new immunotherapy strategies involving therapeutic targeting of HIF-1 molecules associated with hypoxia may enhance the clinical effectiveness of immunotherapy. Targeting hypoxia presents a potential opportunity to improve the clinical benefit of cancer immunotherapy.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Review
Cell Biology
Mykyta Malkov, Chee Teik Lee, Cormac T. Taylor
Summary: Hypoxia and inflammation frequently co-occur in inflammatory diseases, with pro-inflammatory cytokines such as TNF-alpha and IL-1 beta directly impacting HIF signaling pathways. Multi-level crosstalk between inflammatory and hypoxic signaling pathways plays a crucial role in shaping the degree of inflammation at hypoxic sites.
Article
Biochemistry & Molecular Biology
Adrianna Moszynska, Maciej Jaskiewicz, Marcin Serocki, Aleksandra Cabaj, David K. Crossman, Sylwia Bartoszewska, Magdalena Gebert, Michal Dabrowski, James F. Collawn, Rafal Bartoszewski
Summary: The cellular adaptive response to hypoxia is regulated by hypoxia-inducible factors (HIFs), HIF-1 and HIF-2. miRNAs play a significant role in modulating cellular signaling pathways during the hypoxic adaptive response.
Article
Biochemistry & Molecular Biology
Yijie Wang, Xiong Liu, Min Wang, Yu Wang, Shuo Wang, Lai Jin, Min Liu, Jun Zhou, Yan Chen
Summary: This study reveals that UBE3B is an E3 ligase for HIF-2 & alpha; and its depletion inhibits breast cancer progression. UBE3B physically interacts with HIF-2 & alpha; and promotes its polyubiquitination, thereby inhibiting its degradation. UBE3B depletion also inhibits breast cancer cell proliferation, migration, and invasion, and suppresses tumor growth and metastasis.
Article
Oncology
Chang W. Song, Hyunkyung Kim, Haeun Cho, Mi-Sook Kim, Sun-Ha Paek, Heon-Joo Park, Robert J. Griffin, Stephanie Terezakis, Lawrence Chinsoo Cho
Summary: High-dose hypofractionated radiation stimulates anti-tumor immune response but also increases immune suppression mediated by HIF alpha. Inhibition of HIF-1 alpha can restore radiation-induced anti-tumor immunity. Combination of HIF-1 alpha inhibitors with immune checkpoint blocking antibodies may enhance overall anti-tumor immune profile.
Article
Biochemistry & Molecular Biology
Yijie Wang, Xiong Liu, Min Liu, Yu Wang, Shuo Wang, Lai Jin, Min Liu, Jun Zhou, Yan Chen
Summary: It has been found that UBE3B acts as an E3 ligase for HIF-2 alpha, promoting its polyubiquitination and inhibiting its degradation, which plays a crucial role in breast cancer growth and metastasis.
Article
Biochemistry & Molecular Biology
Mengxia Qu, Yang Long, Yuqin Wang, Nan Yin, Xiaotian Zhang, Junjie Zhang
Summary: Autotaxin (ATX), an enzyme involved in generating lysophosphatidic acid (LPA) from lysophosphatidylcholine (LPC), is induced by hypoxia through HIF-2a-dependent mechanism in colon cancer SW480 cells. The induction of ATX during hypoxia promotes cancer cell migration through the ATX-LPA axis. The upregulation of ATX expression is mediated by histone crotonylation, which is induced by HIF-2a through recruiting p300/CBP.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)