期刊
TRENDS IN BIOTECHNOLOGY
卷 38, 期 2, 页码 136-141出版社
CELL PRESS
DOI: 10.1016/j.tibtech.2019.08.002
关键词
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资金
- United States-India Educational Foundation (USIEF), New Delhi
- US Department of State
- National Science Foundation Plant Genome Research Program [1740874]
- USDA National Institute of Food and Agriculture and Hatch Appropriations [PEN04659, 1016432]
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1740874] Funding Source: National Science Foundation
SpCas9 creates blunt end cuts in the genome and generates random and unpredictable mutations through error-prone repair systems. However, a growing body of recent evidence points instead to Cas9-induced staggered end generation, nonrandomness of mutations, and the predictability of editing outcomes using machine learning models.
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