Article
Chemistry, Inorganic & Nuclear
Huimin Zhong, Cong Jiang, Jiahui Zou, Guancheng Zhu, Yanyan Huang
Summary: In this study, CeO2-based porous carbonaceous frameworks (CeO2 PCFs) were used as antioxidant nanozymes to scavenge reactive oxygen species and absorb benzo[a]pyrene. CeO2 PCFs exhibited excellent superoxide dismutase-like and catalase-like activity, effectively eliminating superoxide radical, hydroxyl radical, and hydrogen peroxide. The 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) free radical scavenging assay confirmed the free radical scavenging ability of CeO2 PCFs. Furthermore, CeO2 PCFs with large surface area and porous structure efficiently adsorbed benzo[a]pyrene. The designed CeO2 PCFs can serve as new antioxidant nanozymes for scavenging reactive oxygen species and adsorbing polycyclic aromatic hydrocarbons (PAHs).
INORGANIC CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Bozena Bukowska, Katarzyna Mokra, Jaromir Michalowicz
Summary: Benzo[a]pyrene (B[a]P) is a representative polycyclic aromatic hydrocarbon (PAH) that is commonly found in the environment and human surroundings. It has been shown to have toxic effects on DNA, reproduction, neurology, and development. The metabolism of B[a]P is mediated by cytochrome P450 (CYP P450) and its polymorphism determines the sensitivity to B[a]P. Additionally, B[a]P may facilitate the replication of viruses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Amor Hedfi, Manel Ben Ali, Montaser M. Hassan, Bander Albogami, Samia S. Al-Zahrani, Ezzeddine Mahmoudi, Paraskevi K. Karachle, Melissa Rohal-Lupher, Fehmi Boufahja
Summary: The majority of experimental studies on the effects of pollutants on meiofauna have been conducted in closed systems, with the current study revealing that single PAHs impact meiobenthic nematodes similarly in closed or open systems, with higher toxicity observed for benzo[a]pyrene. Closed systems contaminated with PAHs became organically enriched, resulting in changes in the types and numbers of deposit feeders. The responses of taxa to hydrocarbons in open systems were influenced by caudal morphology, with the effects of PAHs mixtures being both additive and synergistic. Based on this study, open systems are preferred over closed systems for more accurate research outcomes.
ENVIRONMENTAL POLLUTION
(2021)
Article
Chemistry, Multidisciplinary
Jingnan Jin, Yahui Shi, Baozhong Zhang, Dongjin Wan, Qingye Zhang, Ying Li
Summary: In this study, a BaP-degrading strain Pannonibacter sp. JPA3 was isolated from oil well production water, which could remove 80% of BaP at an initial concentration of 100 mg L-1 after 35 days of culture. The metabolites BaP-4,5-dihydrodiol, BaP-4,5-epoxide, 5-hydroxychrysene, and 2-hydroxy-1-naphthoic acid were identified in the biodegradation process. A dioxygenase model was built and molecular docking was performed to investigate the interaction mechanism between BaP and dioxygenase. A hypothesis for the progressive degradation mechanism of BaP by this strain was proposed based on the identification of downstream metabolites. In conclusion, this study provided an efficient BaP degrader and a comprehensive reference for the study of degradation mechanisms at the molecular level.
Article
Environmental Sciences
Barbara Campos Jorge, Ana Carolina Casali Reis, Erika Tissiana Sterde, Paola da Silva Balin, Wellerson Rodrigo Scarano, Hamilton Hisano, Arielle Cristina Arena
Summary: BaP exposure during the prepubertal period negatively impacted the reproductive parameters in adult male rats, leading to compromised copulatory behavior, reduced sperm quality and quantity, and histopathological changes in testicular tissue. A comprehensive risk assessment of prepubertal BaP exposure is necessary to understand its long-term effects on male reproductive health.
Article
Environmental Sciences
Claire Baranger, Isabelle Pezron, Laurence Lins, Magali Deleu, Anne Le Goff, Antoine Fayeulle
Summary: Hydrophobic organic soil contaminants like PAH are poorly mobile in soil and filamentous fungi are efficient in degrading them, but the mechanism of their mobilization needs further investigation. A microfluidic device was designed to study the uptake of BaP by fungi, revealing the uptake and storage mechanisms as well as the release of surface-active compounds.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biotechnology & Applied Microbiology
Mercedes Quintanilla-Mena, Julio Vega-Arreguin, Marcela Del Rio-Garcia, Victoria Patino-Suarez, Santy Peraza-Echeverria, Carlos Puch-Hau
Summary: The study investigated the effects of BaP on the gut microbiome of Oreochromis niloticus. Dysbiosis was observed at 24 hours post-injection, followed by a slight recovery at 72 hours and stabilization at 120 hours. The gut microbiome played a significant role in PAH degradation mainly at 24 hours post-injection.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Environmental Sciences
Rosa Posada-Baquero, Kirk T. Semple, Miguel Ternero, Jose-Julio Ortega-Calvo
Summary: The study utilized desorption extraction method standardized by ISO16751 to determine the bioavailable concentration of the commonly regulated polycyclic aromatic hydrocarbon benzo(a)pyrene (BaP) in industrial soil, showing comparable results to field-contaminated soils.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Multidisciplinary
Xiushang Xu, Amy L. Vonder Haar, Rengo Yoshioka, Qizheng Zhang, Serhii Vasylevskyi, Andrew J. Musser, Akimitsu Narita
Summary: A benzo[rst]pentaphene derivative, BPP-MeOPA, was synthesized and characterized by NMR and single-crystal X-ray analysis. Investigation of its photophysical properties, including transient absorption spectroscopy, revealed improved absorption and emission features from an S-1 state with excitonic and charge-transfer character by introducing MeOPA groups.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Yue Liu, Xinguo Ren, Lixin He
Summary: This study identified six possible DNA adducts with BPQ, including four covalently bound adducts and two adducts purely bound by van der Waals interactions. Surprisingly, the two van der Waals-bound adducts have comparable, if not larger, binding energies than the covalent adducts. These findings provide insights into the interactions between PAH metabolites and DNA.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Pharmacology & Pharmacy
Montserrat Zaragoza-Ojeda, Ulises Torres-Flores, Alejandra Rodriguez-Leviz, Francisco Arenas-Huertero
Summary: This study aimed to assess the effects of BghiP exposure on human cells, and the results showed that BghiP can induce cellular dormancy and ER stress, and regulate cell survival through the increase of pro-inflammatory cytokines.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2022)
Article
Environmental Sciences
Siwar Abouda, Omayma Missawi, Tiziana Cappello, Iteb Boughattas, Giuseppe De Marco, Maria Maisano, Mohamed Banni
Summary: Marine ecosystems are currently facing severe threats from multiple stressors, including microplastics and polycyclic aromatic hydrocarbons. This study assessed the toxicity of environmental relevant concentrations of microplastics and benzo[a]pyrene, and found that they can accumulate in marine organisms and cause cytotoxicity, genotoxicity, and oxidative stress effects.
ENVIRONMENTAL POLLUTION
(2022)
Review
Environmental Sciences
Jiezhang Mo, Doris Wai-Ting Au, Jiahua Guo, Christoph Winkler, Richard Yuen-Chong Kong, Frauke Seemann
Summary: Research indicates that environmental exposure to benzo[a]pyrene may cause osteotoxicity, which could be transgenerationally inherited. Genetic and epigenetic factors play important roles in regulating bone metabolism, and researchers propose two possible epigenetic regulatory circuits in relation to bone miRNAs and genes.
CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yi Zhu, Man Hu, Liqin Yin, Wei Qin, Xiaoyi Hu, Shengmei Lyu, Junfeng Dou
Summary: Proteomics and metabolomics are effective and convenient methods for assessing proteins and metabolites during microbial degradation of pollutants, providing a means to explore degradation pathways. This study investigated the degradation pathways and regulatory characteristics of Microbacterium sp. M.CSW3 for the metabolism of benzopyrene alone and in the presence of phenanthrene and pyrene. Proteomics results identified important enzymes for polycyclic aromatic hydrocarbons (PAHs) metabolism. Metabolomics analysis suggested five degradation pathways for benzopyrene and identified phenanthrene as an intermediate metabolite. The study also showed that the addition of low-molecular-weight PAHs promoted enzyme production and enhanced the degradation of benzopyrene.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2023)
Article
Biochemistry & Molecular Biology
Krystian Mokrzynski, Grzegorz Szewczyk, Tadeusz Sarna
Summary: This study measured the quantum yield and action spectra of singlet oxygen photogeneration by benzopyrenes and their ability to generate superoxide anion. The results revealed the photochemical activity of benzopyrenes in producing singlet oxygen and superoxide anion. Furthermore, the cytotoxicity of benzopyrenes was demonstrated in skin model cells, with concentration-dependent and light-dependent effects, leading to damage to cell mitochondria and increased levels of intracellular reactive oxygen species.
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2023)
Article
Medicine, Research & Experimental
Tyler Risom, Xiaoyan Wang, Juan Liang, Xiaoli Zhang, Carl Pelz, Lydia G. Campbell, Jenny Eng, Koei Chin, Caroline Farrington, Goutham Narla, Ellen M. Langer, Xiao-Xin Sun, Yulong Su, Colin J. Daniel, Mu-Shui Dai, Christiane V. Lohr, Rosalie C. Sears
JOURNAL OF CLINICAL INVESTIGATION
(2020)
Article
Biotechnology & Applied Microbiology
Amita Kashyap, Adelaide Rhodes, Brent Kronmiller, Josie Berger, Ashley Champagne, Edward W. Davis, Mitchell Finnegan, Matthew Geniza, David A. Hendrix, Christiane Loehr, Vanessa M. Petro, Thomas J. Sharpton, Jackson Wells, Clinton W. Epps, Pankaj Jaiswal, Brett M. Tyler, Stephen A. Ramsey
Editorial Material
Veterinary Sciences
Christiane V. Loehr, Scott Hendy, William H. Miller, Patti K. Kiser
JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION
(2020)
Article
Toxicology
Yvonne Chang, Celine Thanh Thu Huynh, Kelley M. Bastin, Brianna N. Rivera, Lisbeth K. Siddens, Susan C. Tilton
TOXICOLOGY IN VITRO
(2020)
Review
Veterinary Sciences
Kristi L. Helke, David K. Meyerholz, Amanda P. Beck, Eric R. Burrough, Rachel J. Derscheid, Christiane Lohr, Elizabeth F. McInnes, Cheryl L. Scudamore, Cory F. Brayton
Summary: Animal models are valuable tools for biomedical researchers, but careful consideration of various features and background factors is necessary for accurate interpretation of results. Different species, breeds/stocks, sex, age, diseases, and other factors can all impact study outcomes.
Article
Agriculture, Dairy & Animal Science
Ibrahim A. Khan, Nathan B. Parker, Christiane V. Lohr, Gita Cherian
Summary: The study found that supplementation of DHA-rich microalgae and methionine in broiler chicken diets can reduce the incidence of breast muscle striping and myopathy, while increasing the content of n-3 fatty acids in meat. However, inclusion of methionine in microalgae-based diets may affect meat tenderness and color.
Article
Pathology
T. William O'Neill, Christiane V. Lohr
Summary: Cutaneous round cell tumors in goats can be challenging to diagnose. This study identified the expression patterns of CD117/KIT and Iba1 in caprine tissues, providing valuable diagnostic markers for cutaneous mast cell tumors and histiocytomas. The research showed that CD117/KIT was predominantly expressed in certain cells, while Iba1 exhibited strong expression in macrophages and other cell types.
VETERINARY PATHOLOGY
(2021)
Article
Pathology
Christiane V. Lohr, Susanne M. Stieger-Vanegas, Jesse L. Terry, Milan Milovancev, Jan Medlock
Summary: The majority of peritumoral lesions identified during in vivo imaging of FISS are non-neoplastic. A protocol targeting PTIIL for microscopic examination showed promising performance but may require further refinement.
VETERINARY PATHOLOGY
(2021)
Article
Chemistry, Medicinal
Yvonne Chang, Julia E. Rager, Susan C. Tilton
Summary: This study evaluated the carcinogenic potential of PAH mixtures through transcriptomic and bioinformatic approaches in a 3D human bronchial epithelial cell model, finding over 3000 genes significantly correlated with RPF values, potential biomarkers for cancer risk evaluation. Analysis of gene sets revealed molecular mechanisms related to PAH cancer risk, linking genes to various cancer-relevant risk values and identifying pathways associated with cell cycle regulation, inflammation, DNA damage, and cell adhesion processes. Additionally, RPF values and IARC categorizations showed similar responses in gene modules, highlighting a novel integration of gene sets with chemical toxicity equivalence estimates for understanding potential mechanisms of PAH-related cancer risk.
CHEMICAL RESEARCH IN TOXICOLOGY
(2021)
Article
Biology
Yang Zhang, Gerd Bobe, Cristobal L. Miranda, Malcolm B. Lowry, Victor L. Hsu, Christiane Lohr, Carmen P. Wong, Donald B. Jump, Matthew M. Robinson, Thomas J. Sharpton, Claudia S. Maier, Jan F. Stevens, Adrian F. Gombart
Summary: XN and TXN were found to reduce hepatic lipid accumulation in mice, with TXN potentially antagonizing the actions of PPAR gamma. They inhibited cell differentiation by decreasing expression of lipogenesis-related genes.
Article
Engineering, Marine
Cara Rodgveller, Christiane Lohr, John A. Dimond
Summary: The study found that capture and time out of water can affect the reflexes and health of sablefish, but these factors did not directly lead to mortality or histological findings. Some reflexes were impaired after capture, but overall there were no significant negative impacts from the capture and experimental conditions.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2021)
Article
Agriculture, Dairy & Animal Science
Ahmad Fraz, Nathan B. Parker, Christiane V. Lohr, Gita Cherian
Summary: This study investigated the effects of organic chromium and flax seed supplementation on broilers. The results showed that the supplementation improved the live performance, carcass yield, muscle lipid profile, histopathological aspects, and meat quality parameters of the broilers.
Article
Veterinary Sciences
Alexandra G. Collins-Webb, Christiane Lohr, Susanne M. Stieger-Vanegas
Summary: Neoplasia of the tubular genital tract in goats is most commonly reported as uterine adenocarcinoma, leiomyoma, or leiomyosarcoma. This retrospective case series analyzed medical records of goats with computed tomography (CT) diagnosis of tubular genital mass and definitive histologic diagnosis of malignant neoplasia. Adenocarcinoma goats had upper urinary tract obstruction, regional lymphadenopathy, peritoneal fluid, and nodules/masses, while leiomyosarcoma goats had mild peritoneal fluid and lymphadenopathy. Five goats had pulmonary nodules, with pathologic confirmation in three cases. Severe lymphadenopathy and obstructive uropathy were observed in goats with adenocarcinoma, but not in those with leiomyosarcoma.
VETERINARY RADIOLOGY & ULTRASOUND
(2023)
Article
Virology
Shelby Matsuoka, Gloria Petri, Kristen Larson, Alexandra Behnke, Xisheng Wang, Muhui Peng, Sean Spagnoli, Christiane Lohr, Ruth Milston-Clements, Konstantin Divilov, Ling Jin
Summary: The newly discovered inhibitor ML324 shows significant inhibitory effect against CyHV-3 infection in koi, providing a new direction for finding treatment methods for CyHV-3 infection in koi.
Article
Education, Scientific Disciplines
Diana M. Hassel, Maria Fahie, Christiane Lohr, Rachel L. Halsey, William Vernau, Elena Gorman
JOURNAL OF VETERINARY MEDICAL EDUCATION
(2020)