Article
Materials Science, Multidisciplinary
Ai-Huei Chiou, Jun-Luo Wei, Ssu-Han Chen
Summary: A novel SERS-based probe utilizing SiNW arrays with AgNPs was developed for capturing Zn2+ ions by BSA. The composite interfaces showed significant Raman scattering enhancement, indicating potential for label-free biosensors with high sensitivity. The specific SERS spectra of BSA and ZnSO4 conjugations suggest promising applications in biosensing.
Article
Microbiology
Fernanda Jimenez Otero, Dianne K. Newman, Leonard M. Tender
Summary: Biofilms provide a protective environment but also present challenges to the cells living within them. Pseudomonas aeruginosa overcomes oxygen limitation by secreting redox active phenazines, which act as electron shuttles. Blocking the re-oxidation of one of these electron shuttles, pyocyanin, decreases cell survival within biofilms and enhances the effect of gentamicin in killing cells. The study highlights the importance of redox cycling of electron shuttles in P. aeruginosa biofilms.
Article
Immunology
Zuoji Ye, Liumei Ye, Dingbin Li, Shunsheng Lin, Wusheng Deng, Li Zhang, Jinhua Liang, Jinlong Li, Qingjun Wei, Ke Wang
Summary: In this study, the researchers found that Daphnetin (DAP) can effectively inhibit and eradicate biofilm formation of Pseudomonas aeruginosa. DAP also reduced bacterial motility and pyocyanin production. These findings suggest that DAP may serve as a natural anti-biofilm therapeutic agent.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Ahmed A. Abdelaziz, Amal M. Abo Kamer, Khaled B. Al-Monofy, Lamiaa A. Al-Madboly
Summary: The discussion focuses on the bioprospecting of microorganisms and their bioactive byproducts, specifically pigments. Microbial pigments, such as pyocyanin produced by Pseudomonas aeruginosa, have various benefits including their natural composition, therapeutic effects, and year-round production. This article concentrates on the production, extraction, and biological applications of pyocyanin pigment in biotechnology, engineering, and biology fields.
MICROBIAL CELL FACTORIES
(2023)
Article
Multidisciplinary Sciences
Chelsey M. VanDrisse, Rosalie Lipsh-Sokolik, Olga Khersonsky, Sarel J. Fleishman, Dianne K. Newman
Summary: This study demonstrates that by engineering the structure of PodA protein and combining it with tobramycin, the killing effect on P. aeruginosa can be enhanced, especially on biofilms that are resistant to conventional antibiotic treatment.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Julie Oziat, Thibaut Cohu, Sylvie Elsen, Maxime Gougis, George G. Malliaras, Pascal Mailley
Summary: Fast identification of bacterial strains is crucial during infections, and electrochemical analysis of culture supernatants can provide a fingerprint of the strain based on the redox peaks observed. Peaks corresponding to known toxins and signals can be identified, along with potential new biomarkers for strain differentiation.
BIOELECTROCHEMISTRY
(2021)
Article
Chemistry, Analytical
Cristina Adrover-Jaume, Antonio Clemente, Barbara Rodriguez-Urretavizcaya, Lluisa Vilaplana, M. Pilar Marco, Estrella Rojo-Molinero, Antonio Oliver, Roberto de la Rica
Summary: Using a paper-based biosensor for competitive immunoassay enables effective detection of PYO in sputum for diagnosing Pseudomonas aeruginosa respiratory infections, with better detection performance and stability compared to traditional ELISA methods.
Article
Microbiology
Rachel L. Neve, Brent D. Carrillo, Vanessa V. Phelan
Summary: The study revealed differences in the metabolism of Pseudomonas aeruginosa, an opportunistic pathogen, when cultured in different artificial sputum media. Concentration of aromatic amino acids and iron from mucin added to the media were found to affect the production of virulence-associated secondary metabolites in P. aeruginosa.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Microbiology
Avi Flamholz, Samuel Saccomano, Kevin Cash, Dianne K. Newman
Summary: From a metabolic perspective, molecular oxygen (O-2) is vital in microbial physiology, but its measurement can be complicated by interactions with bacterial secretions. This study found that certain bacterial metabolites, such as phenazines and flavins, quench phosphorescent O-2 probes, leading to inaccurate measurements. Careful control and custom probe design are necessary for accurate O-2 measurements in the presence of bacteria.
Article
Biotechnology & Applied Microbiology
Adilson Jose da Silva, Josivan de Souza Cunha, Teri Hreha, Kelli Cristina Micocci, Heloisa Sobreiro Selistre-de-Araujo, Blanca Barquera, Mattheos A. G. Koffas
Summary: This study engineered the pyocyanin biosynthetic pathway for high production in a heterologous host for the first time. Optimized culture and induction parameters led to a 3.5-fold increase in pyocyanin accumulation, while pathway balancing through testing plasmids with different copy numbers resulted in a fourfold difference in product titer among the engineered strains. Co-expression of Vitreoscilla hemoglobin Vhb further improved pyocyanin production to a final titer of 18.8 mg/L, demonstrating the potential of using Escherichia coli for phenazines production in electro-fermentation systems.
METABOLIC ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Obinna Markraphael Ajunwa, Olubusola Ayoola Odeniyi, Emmanuel Oluwaseun Garuba, Mrinalini Nair, Enrico Marsili, Abiodun Anthony Onilude
Summary: This study determined the production of pyocyanin, NAD, and NAD synthetase in the electrogenic bacterium Pseudomonas aeruginosa using a Microbial Fuel Cell (MFC). The co-expression of nadD and nadC genes increased the activity of NAD synthetase and resulted in higher pyocyanin concentration, NAD/NADH levels, and MFC potential in PA-A4 strain. This finding advances our understanding of NAD biosynthetic genes in PA electrogenicity.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Selene Garcia-Reyes, Miguel Cocotl-Yanez, Martin Paolo Soto-Aceves, Abigail Gonzalez-Valdez, Luis Servin-Gonzalez, Gloria Soberon-Chavez
Summary: This study characterized the PqsE-dependent production of PYO in Pseudomonas aeruginosa ATCC 9027 and found that PqsE promotes the RhlR-dependent production of PYO, although this production is not strictly dependent on PqsE. The interaction between PqsE and RhlR modifies the transcription of genes involved in PYO production, highlighting the variability of the quorum-sensing response among different P. aeruginosa clades and strains.
MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Theerthankar Das, Biswanath Das, Brandon Clark Young, Vina Aldilla, Shekh Sabir, Basmah Almohaywi, Mark Willcox, Mike Manefield, Naresh Kumar
Summary: The presence of ascorbic acid can interact with pyocyanin, resulting in shifts in its absorbance peaks and structure. This interaction impairs the binding of pyocyanin to DNA and disrupts biofilm formation. In combination with furanone-30, ascorbic acid further inhibits quorum sensing and enhances antibiotic-mediated bacterial killing, reducing the virulence and biofilm formation of Pseudomonas aeruginosa. The antioxidant properties of ascorbic acid play a crucial role in these interactions and their effects.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Electrochemistry
Jeong Hoon Lee, Eun-Tae Yun, Han-Shin Kim, So-Young Ham, Peng-Fei Sun, Yong-Sun Jang, Jeong-Hoon Park, Namsoo Peter Kim, Hee-Deung Park
Summary: In this study, a novel bioelectrochemical reactor was developed using a vertically aligned carbon nanotube composite as an electroconductive barrier to separate microbial cells from hexavalent chromium (Cr(VI)). Through the secretion of an electron shuttle by Pseudomonas aeruginosa, sustainable biological treatment of chromium compounds was achieved without growth inhibition, even at high Cr(VI) concentrations. The reactor exhibited a Cr(VI) removal rate of 99.6% at 100 mg/L, the highest reduction rate achieved using P. aeruginosa.
ELECTROCHIMICA ACTA
(2023)
Article
Microbiology
Yanyan Hu, Wenjing Peng, Yifan Wu, Hui Li, Qi Wang, Huahua Yi, Rong Zhang, Bing Shao, Kui Zhu
Summary: The study demonstrated that the epidemic clones of Pseudomonas aeruginosa in non-cystic fibrosis patients, particularly ST463, exhibited multidrug resistance and carried a variety of virulence genes. These isolates showed high levels of resistance to antibiotics and produced toxins, indicating a high-risk potential for virulence.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Biochemical Research Methods
Natalia E. Markina, Dana Cialla-May, Alexey V. Markin
Summary: The use of cyclodextrins (CDs) in surface-enhanced Raman spectroscopy (SERS) is still developing with less than 100 papers published since 1993. The review reveals controversial information about the efficiency of CDs in SERS analysis, and further experimental investigations are needed to estimate their real potential in SERS-based analysis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Dana Cialla-May, Christoph Krafft, Petra Roesch, Tanja Deckert-Gaudig, Torsten Frosch, Izabella J. Jahn, Susanne Pahlow, Clara Stiebing, Tobias Meyer-Zedler, Thomas Bocklitz, Iwan Schie, Volker Deckert, Juergen Popp
ANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Maher M. Aljohani, Dana Cialla-May, Juergen Popp, Raja Chinnappan, Khaled Al-Kattan, Mohammed Zourob
Summary: Aptamers are RNA/DNA oligonucleotide molecules that specifically bind to targeted molecules. They have potential diagnostic and therapeutic applications for blood diseases and offer advantages over antibodies in terms of selection, production, modification, stability, and immunogenicity. Several aptamers have been developed and investigated in various biomedical areas. The pegaptanib sodium injection, an aptamer-based therapeutic, has been approved by the FDA for therapeutic use. Other aptamers are also in clinical trials. This review highlights the current status and promising aptamers in pre-clinical development for blood diseases.
Article
Materials Science, Multidisciplinary
E. Parinova, S. S. Antipov, V Sivakov, E. A. Belikov, I. S. Kakuliia, S. Yu Trebunskikh, S. Yu Turishchev
Summary: Successful integration of ferritin Dps protein with silicon nanowires has been demonstrated, allowing effective removal of buffer solution salts while retaining ferritin Dps on the porous surface of silicon nanowires.
RESULTS IN PHYSICS
(2022)
Article
Materials Science, Multidisciplinary
D. Yakimchuk, U. Prigodich, S. E. Demyanov, J. Ustarroz, H. Terryn, K. Baert, S. A. Khubezhov, D. Tishkevich, A. Trukhanov, V Sivakov, E. Yu Kaniukov
Summary: Template synthesis is a method for obtaining nanostructures with predetermined shapes and sizes. This study successfully created silver nanostructures with complex geometry by changing only the pore volume of the template without altering other chemical kinetic conditions, and the growth mechanism of these nanostructures was examined and discussed in detail.
MATERIALS CHEMISTRY AND PHYSICS
(2022)
Article
Chemistry, Physical
Tingsen Ming, Benjamin Dietzek-Ivansic, Xiaoju Lu, Xiaohua Zuo, Vladimir Sivakov
Summary: This study investigates the effects of decorating highly n-doped silicon nanowires with silver nanoparticles on hydrogen generation. The results show that the presence of silver nanoparticles can enhance the efficiency of hydrogen generation, and the decorated silicon nanowires can maintain their morphology after hydrogen release.
ACS APPLIED ENERGY MATERIALS
(2022)
Review
Chemistry, Analytical
Stefano Fornasaro, Dana Cialla-May, Valter Sergo, Alois Bonifacio
Summary: This article presents a comprehensive review of various novel approaches based on surface-enhanced Raman scattering (SERS) for the direct and indirect detection and quantification of antibiotic, antifungal, and antituberculosis drugs in different matrices. The challenges for successful exploitation of this technique in the development of assays for point-of-care tests are particularly emphasized.
Article
Pharmacology & Pharmacy
E. Tolstik, M. B. Gongalsky, J. Dierks, T. Brand, M. Pernecker, N. V. Pervushin, D. E. Maksutova, K. A. Gonchar, J. V. Samsonova, G. Kopeina, V. Sivakov, L. A. Osminkina, K. Lorenz
Summary: This study evaluated the effectiveness of drug delivery, release, and toxicity of pSiNPs nanocontainers in colon cancer cells and cardiac cells. The results demonstrated that pSiNPs effectively loaded and localized the drug within cells, and showed good tolerance and potential protective effects against toxicity in cardiac cells.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Dzmitry Yakimchuk, Soslan A. Khubezhov, Uladzislau Prigodich, Daria Tishkevich, Sergei Trukhanov, Alex Trukhanov, Vladimir Sivakov, Egor Y. Kaniukov
Summary: This paper presents a detailed study on the influence of the shape of silver nanostructures on the efficiency of Raman signal amplification. It is found that silver crystals, dendrites, and sunflower-like nanostructures can be used for Raman signal amplification in SiO2/Si template, but with different efficiencies.
Article
Biochemistry & Molecular Biology
Andrey V. Bykov, Tatiana A. Shestimerova, Mikhail A. Bykov, Liubov A. Osminkina, Alexey N. Kuznetsov, Victoria E. Gontcharenko, Andrei V. Shevelkov
Summary: By using X-ray crystal structure analysis, Raman and diffuse reflectance spectroscopies, and quantum chemical calculations, we synthesized a new polyiodoantimonate (HpipeH(2))(2)[Sb2I10](I-2) and investigated its crystal and electronic structure. We found that the compound has a 3D structure with a complex pattern of covalent bonds and non-covalent interactions, which provides a narrow band gap of 1.41 eV. This compound shows potential as a versatile tool for optimizing the band gap of light-harvesting materials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Optics
K. A. Gonchar, E. A. Alekseeva, O. D. Gyuppenen, I. V. Bozhev, E. V. Kalinin, S. A. Ermolaeva, L. A. Osminkina
Summary: In this study, a method of manufacturing composite nanostructures of silicon nanowires decorated with silver (AgSiNWs) was developed, and the SERS activity of AgSiNWs for protein detection was investigated using human serum albumin as an example. The possibility of rapid diagnosis of internalin B (InlB) protein of pathogenic bacteria Listeria monocytogenes by SERS using the obtained nanostructures was shown for the first time. Distinct peaks corresponding to Raman scattering on protein molecules were observed in the spectra of InlB adsorbed on AgSiNWs at different concentrations. The InlB detection limit was calculated to be 4.8 x 10(-9) M based on the experimental data.
OPTICS AND SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Sergey Turishchev, Alexander Schleusener, Olga Chuvenkova, Elena Parinova, Poting Liu, Maxim Manyakin, Sergei Kurganskii, Vladimir Sivakov
Summary: The composition, atomic and electronic structure of a silicon nanowire array coated with tin oxide were studied. The results showed that tin-oxide nanocrystals of 20 nm in size formed a continuous and highly developed surface with a complex phase composition. The use of photoemission electron microscopy in XANES mode revealed that the entire SiNW surface is covered with a tin(IV) oxide layer and traces of tin(II) oxide and metallic tin phases.
Article
Chemistry, Multidisciplinary
Poting Liu, Alexander Schleusener, Gabriel Zieger, Arne Bochmann, Matthijs A. van Spronsen, Vladimir Sivakov
Summary: Tin-containing layers with different degrees of oxidation are uniformly distributed along the length of silicon nanowires formed by a top-down method. The electronic and atomic structure of the obtained layers is investigated using nondestructive X-ray absorption spectroscopy. The distribution effect of tin-containing phases in the nanostructured silicon matrix is demonstrated, and the amount and distribution of tin-containing phases can be varied and controlled by adjusting the geometric parameters.
Article
Cell Biology
Tatiana N. N. Tikhonova, Vasilii S. S. Kolmogorov, Roman V. V. Timoshenko, Alexander N. N. Vaneev, Dana Cohen-Gerassi, Liubov A. A. Osminkina, Petr V. V. Gorelkin, Alexander S. S. Erofeev, Nikolay N. N. Sysoev, Lihi Adler-Abramovich, Evgeny A. A. Shirshin
Summary: In this study, scanning ion conductance microscopy (SICM) was used to investigate the growth of cells on self-assembled peptide-based hydrogels. The results demonstrated that SICM can serve as a tool for studying functional living cells.
Article
Physics, Multidisciplinary
K. A. Gonchar, I. V. Bozh'ev, O. A. Shalygina, L. A. Osminkina
Summary: Due to their unique structural and physical properties, silicon nanowires show great potential in various fields such as electronics, photovoltaics, photonics, sensors, and biomedicine. Among the available methods for synthesizing silicon nanowires, metal-assisted chemical etching is currently one of the most cost-effective approaches. This study uses gold nanoparticles as a catalyst to synthesize silicon nanowires by metal-assisted chemical etching of crystalline silicon. The resulting nanowires have a diameter of about 100 nm, consisting of a crystalline core of about 50 nm in diameter, coated with a SiO2 shell about 25 nm thick, and with silicon nanocrystals at the interface.
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
