Article
Chemistry, Analytical
Tim Boogaerts, Maarten Quireyns, Adrian Covaci, Hans De Loof, Alexander L. N. van Nuijs
Summary: Wastewater-based epidemiology (WBE) uses human metabolic biomarkers in influent wastewater to estimate community-wide exposure to xenobiotics, such as prescription opioids. A sensitive analytical method was optimized for the detection and analysis of 19 opioid biomarkers, allowing for the evaluation of spatial differences in opioid use. The proposed methodology was able to detect most biomarkers above the lower limit of quantification (LLOQ) levels in various locations in Belgium.
Article
Environmental Sciences
Evsen Yavuz-Guzel, Asli Atasoy, Ismail Ethem Goren, Nebile Daglioglu
Summary: The COVID-19 pandemic has significantly impacted mental health. This study utilized wastewater-based epidemiology to monitor changes in antidepressant use during the pandemic, revealing an increase in antidepressant use.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Xiaolin Fan, Jianhe Zhang, Xiaofang Fu, Bo Zhou, Zeqiong Xu, Hongmei Huang, Sheng Han, Xiqing Li
Summary: Synthetic cannabinoids are widely used in many cities in China, and wastewater monitoring can be used to detect their community consumption, but there is currently limited research on this topic.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Haijian Lu, Jingpu Fan, Changsheng Guo, Jiangtao Yang, Heng Zhang, Miao Chen, Yang Liu, Wenxiu Liu, Jian Xu
Summary: Wastewater-based epidemiology is a cost-effective method to determine drug consumption and prevalence in communities. This study estimated the prevalence of antidepressants in five cities in Qinghai Province, China, using wastewater analysis, and examined the feasibility of using this method to estimate depression prevalence.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Analytical
Susan C. Scott, Nicole M. Anders, Morgan Scardina, Christine L. Hann, Michelle A. Rudek
Summary: Vistusertib, an orally bioavailable mTOR inhibitor under clinical trials, was quantitated using a novel LC-MS/MS method to explore exposure-response relationships accurately. The method allowed accurate quantitation of high-concentration samples through dilution, and maintained long-term frozen stability.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Environmental Sciences
Wenting Lin, Zhishan Huang, Shiyu Gao, Zhifeng Luo, Wenxuan An, Ping Li, Senwen Ping, Yuan Ren
Summary: Wastewater-based epidemiology is an effective tool for monitoring drug consumption, but the stability of drugs in wastewater and sewers will significantly affect their residual concentrations. Different drugs exhibit varying degrees of stability under different conditions.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Taja Verovsek, David Heath, Ester Heath
Summary: Enantiomeric profiling provides valuable information on drug residues, enabling the differentiation of drug sources and improving drug consumption estimates. This study developed a chiral derivatisation method for the enantiomeric profiling of amphetamines in wastewater, demonstrating its good performance in terms of accuracy, repeatability, and linearity. The results indicate a correlation between amphetamine residues in wastewater samples and either licit or illicit drug use.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biology
Iris Wagner, Linda Grigoraki, Peter Enevoldson, Michael Clarkson, Sam Jones, Jane L. L. Hurst, Robert J. J. Beynon, Hilary Ranson
Summary: The rapid evaporative ionisation mass spectrometry (REIMS) method can accurately identify the species and age of mosquitoes, with high accuracy in both laboratory-reared and wild populations.
Article
Engineering, Environmental
Richard Bade, Jason M. White, Maulik Ghetia, Santosh Adiraju, Sangeet Adhikari, Lubertus Bijlsma, Tim Boogaerts, Daniel A. Burgard, Sara Castiglioni, Alberto Celma, Andrew Chappell, Adrian Covaci, Erin M. Driver, Rolf U. Halden, Felix Hernandez, Heon-Jun Lee, Alexander L. N. van Nuijs, Jeong-Eun Oh, Marco A. Pineda Castro, Noelia Salgueiro-Gonzalez, Bikram Subedi, Xue-Ting Shao, Viviane Yargeau, Ettore Zuccato, Cobus Gerber
Summary: This study focused on 28 NPS stimulants and detected 11 of them, with eutylone and 3-methylmethcathinone (3MMC) being the most commonly found and with the highest mass loads, indicating international popularity. Older generation stimulants like para-methoxyamphetamine (PMA), methylone, and mephedrone were also detected, showing the dynamic nature of the NPS market. Areas in New Zealand were found to have the highest loads of NPS stimulant consumption, demonstrating the potential public health risks associated with these substances.
ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS
(2022)
Review
Chemistry, Multidisciplinary
Junjie Hu, Fei Liu, Yunlong Chen, Guoqiang Shangguan, Huangxian Ju
Summary: This review summarizes the principle of mass spectrometric sensing, construction of different mass tags for biosensing, and typical combination mode of mass spectrometric imaging (MSI) technique. Future perspectives including the design of portable matching platforms, exploitation of novel mass tags, development of effective signal amplification strategies, and standardization of MSI methodologies are proposed to advance the field of mass spectrometric biosensing.
Article
Environmental Sciences
Sergio Santana-Viera, Pablo A. Lara-Martin, Eduardo Gonzalez-Mazo
Summary: In this study, a method for the simultaneous analysis of 195 prescription, over-the-counter, and illicit drugs was developed using solid phase extraction and liquid chromatography coupled to high resolution mass spectrometry. The method was applied to analyze influent sewage samples from wastewater treatment plants in Cadiz Bay, Spain, and more than 100 pharmaceuticals were quantified, with 19 of them at concentrations higher than 1 μg/L. Wastewater based epidemiology was used to estimate the consumption of the detected compounds in the sampling area.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Chemistry, Analytical
Selina Tisler, David Pattison, Jan H. Christensen
Summary: Matrix effects pose challenges for accurate measurements with LC ESI-MS. This study developed a three-step method to evaluate and compensate for matrix effects in enriched wastewater extracts. The method involved determining the optimal relative enrichment factor, correcting matrix effects at higher REFs, and predicting and correcting residual structure-specific matrix effects.
ANALYTICAL CHEMISTRY
(2021)
Article
Environmental Sciences
Tim Boogaerts, Maarten Quireyns, Hans De Loof, Xander Bertels, Natan Van Wichelen, Bram Pussig, Jan Saevels, Lies Lahousse, Pauline Bonmariage, Wouter Hamelinck, Bert Aertgeerts, Adrian Covaci, Alexander L. N. van Nuijs
Summary: Wastewater-based epidemiology analyzes human metabolic biomarkers in wastewater to study the presence of xenobiotics. It complements existing drug utilization methods and provides objective spatio-temporal information on pharmaceutical consumption in the general population.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Environmental
Nicola Ceolotto, Patricia Dollamore, Angus Hold, Bethany Balne, Kishore Kumar Jagadeesan, Richard Standerwick, Megan Robertson, Ruth Barden, Barbara Kasprzyk-Hordern
Summary: This manuscript presents a novel wastewater-based epidemiology workflow for estimating the prevalence of non-communicable diseases (NCDs) using pharmaceuticals and their metabolites as proxies. The study tested the performance of the SPE-UHPLC-MS/MS method, the stability of biomarkers under various storage conditions, and the consideration of human metabolism in back-calculations. Most analytes exhibited high stability, except for a few exceptions. The method accuracy and precision were found to be satisfactory. The validated method was then applied to estimate pharmaceutical usage in a community wastewater sample and compare the results with prescription data. The new WBE method showed promise in estimating the daily usage/intake of NCD targets.
JOURNAL OF HAZARDOUS MATERIALS
(2024)
Article
Environmental Sciences
Erin M. Driver, Adam J. Gushgari, Joshua C. Steele, Devin A. Bowes, Rolf U. Halden
Summary: This study demonstrates the feasibility of using endogenous biomarkers, specifically glucocorticoid hormones, to estimate population-level stress by monitoring wastewater. The study found reproducible patterns in the excretion of stress hormones, with the highest levels occurring on Mondays and Tuesdays, and the lowest on Sundays. Stress levels were significantly higher on weekdays compared to weekends, and also higher during the first two months of each semester. Overall, stress levels were higher in the Spring semester compared to the Fall semester.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemical Research Methods
Tim Boogaerts, Maarten Quireyns, Florence Maes, Maria Laimou-Geraniou, Natan Van Wichelen, Ester Heath, Bram Pussig, Bert Aertgeerts, Adrian Covaci, Alexander L. N. van Nuijs
Summary: Wastewater-based epidemiology analyzes biomarkers in wastewater to gain evidence-based information about various lifestyles and health aspects of a population. Due to the complexity and trace level occurrence of biomarkers in wastewater, sensitive analytical procedures are crucial. To improve its value, more compounds, locations, and samples need to be analyzed.
DRUG TESTING AND ANALYSIS
(2023)
Article
Environmental Sciences
Stephanie Chaousis, Frederic D. L. Leusch, Colin J. Limpus, Amanda Nouwens, Liesbeth J. Weijs, Antonia Weltmeyer, Adrian Covaci, Jason P. van de Merwe
Summary: All seven species of sea turtle are facing increasing pressures from human activities that are impacting their health. Changes in blood proteins can indicate adverse health outcomes. This study examines the protein abundance in sea turtle plasma, compares protein expression between different populations, and investigates markers of contaminant exposure.
ENVIRONMENTAL RESEARCH
(2023)
Article
Engineering, Environmental
Jing Li, Xiaowen Zeng, Xiaolin Liang, Shuqin Tang, Adrian Covaci, Xiaofeng Ma, Yan Yang, Da Chen
Summary: This study aimed to characterize gestational exposure to plasticizers and antioxidants, and explore the associations with placental function related genes. Several chemicals showed significantly different concentrations between preterm and full-term deliveries, and were associated with mRNA expression levels of selected genes. The associations exhibited chemical-specific patterns and significantly differed between preterm and full-term deliveries.
ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS
(2023)
Article
Biochemical Research Methods
Siebe Lievens, Evelynn Vervoort, Giulia Poma, Adrian Covaci, Mik Van Der Borght
Summary: Concerns about microplastics in the environment have grown, leading scientists to conduct thorough studies. A cryogenic grinding and wet-sieving method was developed to efficiently produce and fractionate microplastics. With this method, polyvinyl chloride (PVC) microplastics could be produced with specific particle sizes and easily separated into different size ranges. The method has the potential to be applied to other types of plastics as well.
METHODS AND PROTOCOLS
(2023)
Article
Chemistry, Analytical
Chien-chia Chen, Karabi Mondal, Philippe Vervliet, Adrian Covaci, Evan P. O'Brien, Karl J. Rockne, James L. Drummond, Luke Hanley
Summary: Compound identification by database searching is commonly used in mass spectrometric data analysis. Machine learning was applied to statistically evaluate the identification results generated by software. Logistic regression was used to assign identification probabilities for each compound, providing more confidence in identification beyond database matching.
ANALYTICAL CHEMISTRY
(2023)
Article
Toxicology
Elias Iturrospe, Rani Robeyns, Katyeny Manuela da Silva, Maria van de Lavoir, Joost Boeckmans, Tamara Vanhaecke, Alexander L. N. van Nuijs, Adrian Covaci
Summary: Despite being common, alcoholic liver disease remains poorly understood, especially in its early stages. In this study, alcoholic steatohepatitis was simulated in vitro to investigate the metabolic alterations and identify affected pathways. The researchers discovered key findings, such as the influence of TNF-alpha on hepatic triglycerides and the detection of FAHFA-containing triglycerides in human hepatocytes, which may play an important role in the progression of alcoholic steatohepatitis. Additionally, ethoxylated phosphorylcholine was identified as a potential new biomarker of ethanol exposure.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Environmental Sciences
Shanshan Yin, Thomas J. McGrath, Adam Cseresznye, Jasper Bombeke, Giulia Poma, Adrian Covaci
Summary: Chlorinated paraffins (CPs) are a major environmental concern and it is essential to have an effective tool for monitoring personal exposure to CPs. In this study, silicone wristbands (SWBs) were used as a personal passive sampler, and results showed that micro-environments were key contributors to dermal exposure to CPs.
ENVIRONMENTAL RESEARCH
(2023)
Article
Chemistry, Analytical
Katyeny Manuela da Silva, Michele Woelk, Palina Nepachalovich, Elias Iturrospe, Adrian Covaci, Alexander L. N. van Nuijs, Maria Fedorova
Summary: Epilipids, a subset of the lipidome, play a crucial role in regulating redox lipid metabolism and influencing cell fate decisions. Ion mobility spectrometry (IMS) combined with liquid chromatography-high-resolution mass spectrometry (LC-HRMS) can improve the peak capacity and separation ability for the analysis of oxidized complex lipids. This study demonstrates the advantages and limitations of implementing IMS in LC-HRMS workflows and provides insights into the annotation of oxidized complex lipids.
ANALYTICAL CHEMISTRY
(2023)
Article
Environmental Sciences
Lidia Belova, Giulia Poma, Maarten Roggeman, Yunsun Jeong, Da-Hye Kim, Patrick Berghmans, Jan Peters, Amina Salamova, Alexander L. N. van Nuijs, Adrian Covaci
Summary: Quaternary ammonium compounds (QACs) in European indoor dust were identified, characterized, and semi-quantified using ion-mobility high-resolution mass spectrometry. Targeted QACs (21) and suspect QACs (17) were detected with high detection frequencies. The concentrations of individual QACs were estimated and daily intakes for adults and toddlers were calculated. This study provides important insights into the potential human exposure to QACs.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Environmental Sciences
Thomas J. McGrath, Giulia Poma, Sebastien Hutinet, Yukiko Fujii, Robin E. Dodson, Boris Johnson-Restrepo, Dudsadee Muenhor, Gaud Dervilly, Ronan Cariou, Adrian Covaci
Summary: In this study, dust samples from Japan, Australia, Colombia, and Thailand were analyzed for different chain lengths of chlorinated paraffins. The results showed that chlorinated paraffins were detected in all samples, with the highest concentrations found in samples from Colombia and Thailand. Based on current toxicological data, it was determined that short-chain and medium-chain chlorinated paraffins in indoor dust do not pose a health risk through ingestion.
ENVIRONMENTAL POLLUTION
(2023)
Article
Environmental Sciences
Elisabeth Hansen, Jiachen Sun, Bjorn Helander, Jan Ove Bustnes, Igor Eulaers, Veerle L. B. Jaspers, Adrian Covaci, Marcel Eens, Sophie Bourgeon
Summary: English Summary: Exposure to persistent organic pollutants (POPs), such as organochlorines (OCs) and polybrominated diphenyl ethers (PBDEs), can have adverse health effects on wildlife. White-tailed eagles in the Baltic ecosystem have suffered population declines due to severe exposure to DDT and PCB. In this study, feathers from breeding WTE pairs were analyzed to investigate the annual variations in feather corticosterone (fCORT), POPs, and dietary proxies. The results did not support fCORT as a relevant biomarker of contaminant-mediated effects in WTEs, but fCORT can be used as a non-destructive assessment of long-term stress physiology in wild raptors.
ENVIRONMENTAL RESEARCH
(2023)
Article
Environmental Sciences
Paulien Cleys, Lucas Panneel, Jasper Bombeke, Catalina Dumitrascu, Govindan Malarvannan, Giulia Poma, Antonius Mulder, Philippe G. Jorens, Adrian Covaci
Summary: This study reports metabolites of phthalate and alternative plasticizers in neonatal hair samples as biomarkers for exposure to these plasticizers. Results showed that hair sampled from premature neonates after a NICU stay contained significantly higher metabolite concentrations of both phthalates and alternative plasticizers compared to healthy control neonates. Prolonged NICU exposure to respiratory support devices and gastric tubes was correlated with increased concentrations in hair samples.
ENVIRONMENTAL RESEARCH
(2023)
Article
Environmental Sciences
Valentina Rosolen, Elisa Giordani, Marika Mariuz, Maria Parpinel, Vicente Mustieles, Liese Gilles, Eva Govarts, Laura Rodriguez Martin, Kirsten Baken, Greet Schoeters, Ovnair Sepai, Eva Sovcikova, Lucia Fabelova, Jiri Kohoutek, Tina Kold Jensen, Adrian Covaci, Maarten Roggeman, Lisa Melymuk, Jana Klanova, Argelia Castano, Marta Esteban Lopez, Fabio Barbone
Summary: The knowledge about the effects of organophosphate flame retardants on children's neurodevelopment is limited. This research aimed to evaluate the association between exposure to these flame retardants and children's neurodevelopment. The study found a small, negative trend between neurodevelopment and exposure to BDCIPP and DPHP, but the evidence is limited. Further investigations are needed to explore the timing of exposure and the effect of other metabolites.
Article
Engineering, Environmental
Anran Cai, Sylvie Remy, Virissa Lenters, Bianca Cox, Greet Schoeters, Adrian Covaci, Roel Vermeulen, Lutzen Portengen
Summary: Childhood exposure to endocrine-disrupting chemicals (EDCs) may affect metabolic outcomes. Our study found associations between certain chemicals and body mass index, abdominal obesity, total cholesterol, and triglycerides. Further investigation is needed for sex-specific effects.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).