期刊
SENSORS
卷 19, 期 18, 页码 -出版社
MDPI
DOI: 10.3390/s19183882
关键词
antibiotics; smartphone; food; biosensors; bioluminescence; milk; whole-cell biosensor
资金
- Innovative Materials and Analysis Technology Exploration Program (The i-MATE Program) of Academia Sinica Taiwan [AS-iMATE-107-96]
- Ministry of Science and Technology, Taiwan [MOST 107-2113-M-001-024]
- Academia Sinica-Hebrew University Joint Research Program on Nanoscience and Nanotechnology
- Minerva Center for Bio-Hybrid Complex Systems
- NATO Science for Peace and Security Program [985042]
We present an integral smartphone-based whole-cell biosensor, LumiCellSense (LCS), which incorporates a 16-well biochip with an oxygen permeable coating, harboring bioluminescent Escherichia coli bioreporter cells, a macro lens, a lens barrel, a metal heater tray, and a temperature controller, enclosed in a light-impermeable case. The luminescence emitted by the bioreporter cells in response to the presence of the target chemicals is imaged by the phone's camera, and a dedicated phone-embedded application, LCS_Logger, is employed to calculate photon emission intensity and plot it in real time on the device's screen. An alert is automatically given when light intensity increases above the baseline, indicating the presence of the target. We demonstrate the efficacy of this system by the detection of residues of an antibiotic, ciprofloxacin (CIP), in whole milk, with a detection threshold of 7.2 ng/mL. This value is below the allowed maximum as defined by European Union regulations.
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