Article
Veterinary Sciences
Oliver W. Stringer, Janine T. Bosse, Sonia Lacouture, Marcelo Gottschalk, Laszlo Fodor, Oystein Angen, Eduardo Velazquez, Paul Penny, Liancheng Lei, Paul R. Langford, Yanwen Li
Summary: This study validated the use of Whatman FTA(R) cards for collection and processing of A. pleuropneumoniae isolates or porcine lung tissue samples, allowing for rapid, low-cost identification and serotyping of the pathogen.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Multidisciplinary Sciences
Xuexiang Lin, Xiao-Yu Liu, Bo Zhang, Ai-Qing Qin, Kwok-Min Hui, Kevin Shi, Yang Liu, Don Gabriel, X. James Li
Summary: A virus enzyme assay using a luciferin derivative as the substrate has been developed as an alternative method for detection of acute viral infection, particularly for influenza diagnosis. The assay showed similar detection rates for all influenza types and subtypes except for A(H7N9) due to lower viral titer. When tested with diluted specimens in comparison to a real-time polymerase chain reaction assay, the assay demonstrated high sensitivity and specificity.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Xue-Lian Luo, Xiu-Dan Zhang, Bei-Jie Li, Tian Qin, Zhi-Jie Cao, Qian-Jin Fan, Jing Yang, Dong Jin, Shan Lu, Ya-Yun Zheng, Xue-Fang Xu, Ji Pu, Jianguo Xu
Summary: Lassa virus is an important human pathogen causing hemorrhagic fever in West Africa, with an increasing risk of imported cases to other countries due to increased traveling. The high nucleotide diversity of the virus strains complicates the development of diagnostic assays. The GPC RT-PCR/2007 assay and the Mabsky kit showed good sensitivity and specificity for detecting most LASV strains.
MICROBIOLOGY SPECTRUM
(2023)
Article
Medicine, Research & Experimental
Yushen Jiang, Shanming Zhang, Hong Qin, Shuai Meng, Xuyi Deng, He Lin, Xiaoliang Xin, Yuxin Liang, Bowen Chen, Yan Cui, Yiheng Su, Pei Liang, Guangzhi Zhou, Hongbo Hu
Summary: A quantitative RT-PCR method for detecting the COVID-19 virus was developed in this study, with acceptable performance and reliable evaluation of viral loads with low detection limits.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2021)
Article
Fisheries
Ifakat Tulay Cagatay
Summary: The study evaluated the feasibility of using FTA (R) card for sampling infected rainbow trout tissues and significantly accelerated the molecular diagnosis of various bacterial diseases. The results showed that DNA extraction from FTA (R) card is efficient and cost-effective for rapid molecular diagnosis of bacterial fish pathogens.
AQUACULTURE INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Naima Ahmed Fahmi, Heba Nassereddeen, Jaewoong Chang, Meeyeon Park, Hsinsung Yeh, Jiao Sun, Deliang Fan, Jeongsik Yong, Wei Zhang
Summary: AS-Quant is a robust program for identifying alternative splicing events from RNA-seq data. It outperforms other widely used tools by calculating read coverage, categorizing event types, and generating short read coverage plots. The significant alternative splicing events identified by AS-Quant were validated using RT-PCR experiments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Danna Zhou, Shuangshuang Wang, Keli Yang, Xue Liu, Wei Liu, Rui Guo, Wan Liang, Fangyan Yuan, Zewen Liu, Ting Gao, Yong-xiang Tian
Summary: A new real-time nucleic acid sequence-based amplification (RT-NASBA) method was developed for rapid detection of Japanese encephalitis virus (JEV), showing high specificity and sensitivity.
MICROBIAL PATHOGENESIS
(2021)
Article
Infectious Diseases
Ming Liao, Jianmin Wu, Manman Dai, Huanan Li, Nan Yan, Runyu Yuan, Chungen Pan
Summary: A convenient and safe method for rapid detection of SARS-CoV-2 in samples was developed, which can be used to identify animals susceptible to infection and determine the virus status in samples, as well as for developing assays for detecting other viruses.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Medicine, General & Internal
Antonella Mencacci, Alessio Gili, Anna Gidari, Elisabetta Schiaroli, Carla Russo, Elio Cenci, Barbara Camilloni, Alessandro Graziani, Arduino Melelli-Roia, Daniela Francisci, Fabrizio Stracci
Summary: In patients diagnosed with SARS-CoV-2 infection at least 21 days before, viable virus can still be found, especially in those with concomitant diseases. The Lumipulse(R) Antigen Assay (LAA) is a better predictor of non-infectivity compared to RT-PCR, and can safely be used to end isolation in cases with long persistence of viral RNA in the respiratory tract.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Agriculture, Dairy & Animal Science
Fadila Abosrer, Giulia Pezzoni, Emiliana Brocchi, Anna Castelli, Stefano Baselli, Santina Grazioli, Hafsa Madani, Elfurgani Kraim, Abdunaser Dayhum, Ibrahim Eldaghayes
Summary: Foot-and-mouth disease (FMD) is a highly contagious viral disease that affects cloven-hoofed domestic and wild animals. Diagnosing and controlling FMD outbreaks is crucial, and using FTA cards can be a cost-effective method for sending samples for diagnosis.
Article
Chemistry, Analytical
Guijun Miao, Meng Guo, Ke Li, Xiangzhong Ye, Michael G. Mauk, Shengxiang Ge, Ningshao Xia, Duli Yu, Xianbo Qiu
Summary: Convective PCR (CPCR) utilizes thermal convection to continuously drive reagent in a capillary tube, enabling spatially separate melting, annealing, and extending. By incorporating an FTA membrane filter, CPCR constructs a single reactor for both sample preparation and amplification. The system performance is optimized by analyzing critical issues such as wash scheme and reaction temperature.
Article
Biotechnology & Applied Microbiology
Hyuna Park, Wonjong Jung, Hyeongseok Jang, Kak Namkoong, Kwon-Young Choi
Summary: This study developed a simple and rapid thermolysis method that can bypass the need for viral RNA extraction and purification. The method integrates thermolysis, RT, and digital PCR into a single chip for large-scale viral analysis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Daniel Plante, Julio Alexander Bran Barrera, Maude Lord, Irene Iugovaz, Neda Nasheri
Summary: A method for extracting viral RNA from raw oysters has been developed, showing high efficiency in removing RT-PCR inhibitors and reliably detecting viral contamination. The method outperformed the ISO 15216-1 method and showed comparable efficiency to Method E, with fewer steps and shorter hands-on time.
Article
Horticulture
Chang Ho Ahn, Jung Yeon Han, Hyeong Soo Park, Hyun Won Yoon, Jung Won Shin, Jeong Min Seo, Hana Lee, Yeoung Ryul Kim, Saeng Geul Baek, Jae Ik Nam, Jung Min Kim, Yong Eui Choi
Summary: In this study, transcriptomic analysis identified three genes (TkBBM, TkWOX, and TkSERK) that are involved in somatic embryogenesis of Thuja koraiensis Nakai. These genes were highly expressed during somatic embryogenesis and may play important roles in this process. The findings of this study can be used as a reference for comparative transcriptome analysis in related species and further functional genomics studies in conifers.
Article
Biochemical Research Methods
Xiaoqin Li, Wenli Hu, Yu Li, Yan Li, Suiyun Chen, Jianguang Wang
Summary: In this study, a rapid, specific, sensitive, and low-cost RT-LAMP method was developed for the detection of Maize yellow mosaic virus (MaYMV). The RT-LAMP assay demonstrated 100 times higher sensitivity than RT-PCR and successfully detected MaYMV using crude RNA extracted from infected maize as a template.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Virology
Jaime A. Cardona-Ospina, Kovy Arteaga-Livias, Wilmer E. Villamil-Gomez, Carlos E. Perez-Diaz, D. Katterine Bonilla-Aldana, Alvaro Mondragon-Cardona, Marco Solarte-Portilla, Ernesto Martinez, Jose Millan-Onate, Eduardo Lopez-Medina, Pio Lopez, Juan-Carlos Navarro, Luis Perez-Garcia, Euler Mogollon-Rodriguez, Alfonso J. Rodriguez-Morales, Alberto Paniz-Mondolfi
Summary: The COVID-19 pandemic has rapidly spread throughout Latin America, overlapping infections with other viruses like Dengue may lead to increased ICU admissions. Colombia has confirmed a significant number of Dengue and COVID-19 cases in the first 20 epidemiological weeks of 2020.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Jaime A. Cardona-Ospina, Diana M. Rojas-Gallardo, Sandra C. Garzon-Castano, Erika V. Jimenez-Posada, Alfonso J. Rodriguez-Morales
Summary: Phylogenetic methods have become increasingly important in the study of emerging infectious diseases, providing valuable tools for understanding pathogen dynamics and developing vaccines and antiviral drugs. The application of phylodynamic analysis during the COVID-19 pandemic can enhance our understanding of current dynamics and guide the design of vaccines and antiviral drugs.
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2021)
Review
Medicine, Research & Experimental
Augusto Zuluaga-Velez, Adrian Quintero-Martinez, Lina M. Orozco, Juan C. Sepulveda-Arias
Summary: Silk fibroin nanocomposites have great potential in tissue engineering, with the ability to enhance stability and mechanical performance by incorporating inorganic phases and influencing specific biological responses. However, challenges still exist in their application to other tissues, calling for further research on the interaction between inorganic and organic phases and the associated biological responses.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Review
Medicine, Research & Experimental
Nancy Y. Guerrero-Pepinosa, Maria C. Cardona-Trujillo, Sandra C. Garzon-Castano, Luz Angela Veloza, Juan C. Sepulveda-Arias
Summary: This systematic review provides evidence on the antiproliferative and antitumor activities of thiazole and oxazole compounds and their derivatives from 2014 to April 2020. Most of the promising compounds identified contained thiazole nuclei or derivatives.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Food Science & Technology
Leydy Lorena Mendoza-Tobar, Ivonne Alejandra Meza-Cabrera, Juan C. Sepulveda-Arias, Jimmy Alexander Guerrero-Vargas
Summary: This research studied the pathophysiological effects of venom from two dangerous scorpion species in Colombia, revealing that the venoms can cause significant histopathological alterations in main organs like the heart and lungs, leading to multiple organ failure, as well as inducing hyperglycemia. The severity of scorpionism symptoms ranged from mild to severe, affecting the autonomous nervous system.
Article
Veterinary Sciences
Lyda Caballero Mendez, Luz Natalia Franco Montoya, Margarita Maria Mazo, Juan Carlos Sepulveda, Esteban Valencia, Tatiana Portilla, Leandro Restrepo
Summary: This study aimed to determine the prevalence of M. haemofelis infection and associated risk factors in domestic felines in Pereira, Colombia. Analysis of blood samples from 104 cats revealed a high infection rate, with a significant correlation between age and infection.
REVISTA DE INVESTIGACIONES VETERINARIAS DEL PERU
(2022)
Article
Public, Environmental & Occupational Health
Jaime A. Cardona-Ospina, Victoria Stittleburg, Natalia Millan-Benavidez, Juliana Restrepo-Chica, Autum Key, Diana Marcela Rojas-Gallardo, Anne Piantadosi, Matthew H. Collins, Jesse J. Waggoner
Summary: This study tested the stability of arboviral RNA using contrived dried blood spots as a means of sample collection and storage. The results showed that RNA of dengue virus, chikungunya virus, and Zika virus could be detected up to 180 days using protein saver cards, making them suitable for diagnostic RT-PCR assays and next generation sequencing.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2022)
Article
Infectious Diseases
Jaime A. Cardona-Ospina, Adriana M. Trujillo, Erika V. Jimenez-Posada, Juan C. Sepulveda-Arias, Fredy A. Tabares-Villa, Joanne S. Altieri-Rivera, Alejandro Monsalve, Juliana Restrepo-Chica, Daniela Osorio, Daniel Espinoza, Yerun Zhu, Juan D. Castrillon-Spitia, Valentina Henao-SanMartin, David R. Murillo-Garcia, Natalia Millan, Sandra X. Olaya, Ana M. Valencia-Montoya, Hugo A. Bedoya-Arias, Rhuvi Villamizar-Pena, Estefania Gutierrez-Ocampo, Yeimer Holguin-Rivera, Isabella Cortes-Bonilla, Maria C. Cardona-Trujillo, Alejandra Garcia-Barco, D. Katterine Bonilla-Aldana, Guillermo J. Lagos-Grisales, Alfonso J. Rodriguez-Morales, Matthew H. Collins
Summary: A high proportion of pregnant women in a region endemic for Aedes-borne viruses, including the teratogenic Zika virus, remain susceptible, suggesting a risk for future epidemics.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Agriculture, Dairy & Animal Science
D. Katterine Bonilla-Aldana, Erwin J. Gutierrez-Grajales, Daniela Osorio-Navia, Mariana Chacon-Pena, Adrian E. Trejos-Mendoza, Soffia Perez-Vargas, Lorenzo Valencia-Mejia, Luisa F. Marin-Arboleda, J. Paola Martinez-Hidalgo, Maria Angelica Reina-Mora, Luz Victoria Gonzalez-Colonia, Jaime A. Cardona-Ospina, Erika Vanessa Jimenez-Posada, Diego Andres Diaz-Guio, Jean Carlos Salazar, Manuel Sierra, Fausto Munoz-Lara, Lysien Zambrano, Eduardo Ramirez-Vallejo, Juan Camilo Alvarez, Ingrid Lorena Jaramillo-Delgado, Samuel Pecho-Silva, Alberto Paniz-Mondolfi, Alvaro A. Faccini-Martinez, Alfonso J. Rodriguez-Morales
Summary: The study found that dogs infected with canine vector-borne diseases commonly exhibit thrombocytopenia, neutropenia, and pancytopenia, highlighting the importance of haematology in diagnosing diseases. These haematological alterations can serve as key indicators for ehrlichiosis, anaplasmosis, and other related diseases, especially in endemic areas.
Article
Engineering, Biomedical
Augusto Zuluaga-Velez, Carlos Andres Toro-Acevedo, Adrian Quintero-Martinez, Jhon Jairo Melchor-Moncada, Francisco Pedraza-Ordonez, Enrique Aguilar-Fernandez, Juan Carlos Sepulveda-Arias
Summary: Silk fibroin hydrogels fabricated from Colombian hybrid cocoons showed excellent performance in the regeneration of hyaline cartilage, promoting cell proliferation and deposition. These hydrogels could also be used as a source of biomedical devices.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2022)
Article
Biology
Carlos Isaza, Oscar Mauricio Castano-Ramirez, Juan Pablo Velez, Julieta Henao, Leonardo Beltran-Angarita, Juan Carlos Sepulveda-Arias
Summary: Compared to conventional methadone maintenance treatment, using the CYP2B6 516G>T polymorphism to calculate methadone dosage resulted in improved retention rates, reduced heroin usage, and improved quality of life for patients.
Article
Microbiology
Carlos A. Naranjo-Galvis, Rima Mcleod, Jorge Enrique Gomez-Marin, Alejandra de-la-Torre, Cristian Rocha-Roa, Nestor Cardona, Juan Carlos Sepulveda-Arias
Summary: Ocular toxoplasmosis (OT) is characterized by inflammation within the eye. This study identified a significant association between the Gln460Arg single-nucleotide polymorphism (SNP) in the P2RX7 gene and OT in Colombian patients. This SNP could potentially serve as a biomarker for identifying Colombian patients at risk of OT.
Article
Public, Environmental & Occupational Health
D. Katterine Bonilla-Aldana, Erwin J. Gutierrez-Grajales, J. Paola Martinez-Arboleda, Maria Angelica Reina-Mora, Adrian E. Trejos-Mendoza, Soffia Perez-Vargas, Lorenzo Valencia-Mejia, Luisa F. Marin-Arboleda, Daniela Osorio-Navia, Mariana Chacon-Pena, Luz Victoria Gonzalez-Colonia, Jaime A. Cardona-Ospina, Erika Vanessa Jimenez-Posada, Andres Diaz, Jean Carlos Salazar, Manuel Sierra, Fausto Munoz-Lara, Lysien Zambrano, Eduardo Ramirez-Vallejo, Juan Camilo Alvarez, Ingrid Lorena Jaramillo-Delgado, Samuel Pecho-Silva, Alberto Paniz-Mondolfi, Alvaro A. Faccini-Martinez, Alfonso J. Rodriguez-Morales
Summary: A serosurvey conducted in Pereira, Colombia revealed a high seroprevalence of tick-borne diseases in local dogs, with E. canis/ewingii being the most common infection followed by A. phagocytophilum/platys. Dogs from lower socioeconomic families were more likely to be infected, and anorexia and mucocutaneous paleness were significantly associated with higher seroprevalence.
PARASITE EPIDEMIOLOGY AND CONTROL
(2022)
Meeting Abstract
Public, Environmental & Occupational Health
Teresa C. Smith, Daniel O. Espinoza, Yerun Zhu, Sylvia Becker-Dreps, Natalie M. Bowman, Srilatha Edupuganti, Alfonso J. Rodriguez-Morales, Jaime A. Cardona-Ospina, Filemon Bucardo, Aravinda M. de Silva, Matthew H. Collins
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2021)