Article
Fisheries
Dayanne Carla Fernandes, Silas Fernandes Eto, Gustavo da Silva Claudiano, Paulo Fernandes Marcusso, Fernando Carlos Ramos-Espinoza, Julieta Rodini Engracia de Moraes
Summary: This study aimed to obtain purified polyclonal immunoglobulin Y (IgY) for the diagnosis of A. hydrophila by immunizing chickens and demonstrated the utility of IgY in detecting A. hydrophila within the phagosomes of phagocytes and extracellular bacteria colonizing host tissues in pacus.
AQUACULTURE RESEARCH
(2022)
Article
Environmental Sciences
Analia Ale, Juan M. Galdoporpora, Maria C. Mora, Fernando R. de la Torre, Martin F. Desimone, Jimena Cazenave
Summary: This study aimed to evaluate the mitigatory effect of humic acids (HAs) on silver nanoparticles (AgNPs) toxicity. The results showed that the release of Ag+ from AgNPs decreased when HAs were present in the media, and Ag accumulation in fish tissue was lower with the addition of HAs. Additionally, antioxidant enzymes and other indicators were mitigated by HAs presence in the media under exposure to both forms of silver.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Fisheries
Marco Medina-Morillo, Giovanna Sotil, Caren Arteaga, Gabriela Cordero, Mauricio Laterca Martins, German Murrieta-Morey, Jefferson Yunis-Aguinaga
Summary: The aim of this study was to isolate and identify different species of Aeromonas and evaluate their lethality in Piaractus brachypomus. The study found that P. brachypomus was susceptible to various species of Aeromonas, each carrying different virulence genes.
AQUACULTURE REPORTS
(2023)
Article
Food Science & Technology
Jing Dong, Tianhui Yan, Qiuhong Yang, Yi Song, Bo Cheng, Shun Zhou, Yongtao Liu, Xiaohui Ai
Summary: Aeromonas hydrophila is a common pathogen in freshwater aquaculture that can also cause health problems in humans. Antibiotic-resistant strains of A. hydrophila are a concern as they limit antibiotic usage and can lead to treatment failure. Additionally, antibiotic residues in aquatic products pose a risk to quality and safety. This study explores alternative strategies to combat infections caused by antibiotic-resistant bacteria, focusing on palmatine, a compound that inhibits the expression of a key virulence factor in A. hydrophila. The findings suggest that palmatine could be a promising treatment for A. hydrophila-associated infections in aquaculture.
Article
Fisheries
Milena Freitas, Lieschen V. G. Lira, Raquel B. Ariede, John F. G. Agudelo, Rubens Ricardo de Oliveira Neto, Carolina H. S. Borges, Vito A. Mastrochirico-Filho, Baltasar F. Garcia Neto, Roberto Carvalheiro, Diogo T. Hashimoto
Summary: The study estimated genetic parameters, evaluated genotype by environment interaction, and identified morphotypes for growth traits in pacu using data from a pedigreed population in Brazil. The results showed moderate to high heritability estimates, positive genetic correlations between final weight and morphometric traits, and different morphotype patterns for pacu. GxE interaction was considered low for all traits between the two environments.
Article
Biochemistry & Molecular Biology
Iuri Moraes Neyrao, Jaqueline Dalbello Biller, Leonardo Susumu Takahashi, Elisabeth Criscuolo Urbinati
Summary: The study investigated the effects of oral hydrocortisone and acute stressor on stress, immune responses, and antioxidant system responses in juvenile Piaractus mesopotamicus. The findings showed that exposure to stressor and HC impacted circulating glucose, cortisol levels, and immune responses, while differences in biomarkers indicated that exogenous hormone was unable to precisely replicate stress responses.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2021)
Article
Biology
Zhao Lv, Yazhou Hu, Jin Tan, Xiaoqing Wang, Xiaoyan Liu, Cong Zeng
Summary: The dominant disease in the aquaculture of Chinese soft-shelled turtle is hemorrhagic sepsis caused by Aeromonas hydrophila infection. This study identified two groups of turtles with different susceptibility to A. hydrophila and examined their gene expression profiles post-infection, providing new insights into immune mechanisms against A. hydrophila.
Article
Veterinary Sciences
Jing Dong, Tianhui Yan, Qiuhong Yang, Shun Zhou, Yi Song, Yongtao Liu, Liang Ma, Ning Xu, Yibin Yang, Xiaohui Ai
Summary: The fast-growing demand for aquatic products has led to the rapid development of aquaculture. However, antibiotic resistance caused treatment failure in the control of diseases caused by bacterial pathogens. In this study, researchers identified aerolysin as a target for developing drugs and found that polydatin could decrease the pathogenesis of antibiotic-resistant A. hydrophila infections.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Fisheries
Milena Freitas, Celma G. Lemos, Raquel B. Ariede, John F. G. Agudelo, Rubens R. O. Neto, Carolina H. S. Borges, Vito A. Mastrochirico-Filho, Fabio Porto-Foresti, Rogerio L. Iope, Fabricio M. Batista, Jose R. F. Brega, Diogo T. Hashimoto
Summary: This study developed a computer vision system (CVS) using deep learning to predict body shape in fish. Through the analysis of pacu, it was found that morphotypes vary according to the environment, sex, and age. The genetic parameters for body shape indicate that this trait is moderately controlled by genetics and can be altered through selection.
Article
Biotechnology & Applied Microbiology
Chaojie Wang, Shengchi Shi, Meiju Wei, Yi Luo
Summary: This study reports the discovery of an A. hydrophila prophage endolysin, PlyD4, which exhibits antibacterial activity against a broad range of pathogens. PlyD4 shows bactericidal effects in vitro and can protect zebrafish from A. hydrophila infection in vivo.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Jing Dong, Lushan Zhang, Yongtao Liu, Shun Zhou, Yibin Yang, Ning Xu, Qiuhong Yang, Xiaohui Ai
Summary: Aeromonas hydrophila is a widely distributed pathogen that has developed antibiotic resistance, necessitating the exploration of novel treatment strategies. The bacterium's pathogenicity is primarily attributed to virulence factors such as aerolysin and biofilm.
Article
Fisheries
Aldo Aparecido Proietti-Junior, Luciana Sampaio Lima, Emily Moraes Roges, Yan Correa Rodrigues, Karla Valeria Batista Lima, Dalia Prazeres Rodrigues, Marcos Tavares-Dias
Summary: This study reported an outbreak of disease in pirarucu caused by Aeromonas, conducted experimental co-infection research, and characterized resistance profile, virulence factors, and phenotypic and molecular differentiation. The findings support the existence of synergism between A. hydrophila and A. jandaei in pirarucu, with implications for occurrences of this co-infection and determinants of virulence.
AQUACULTURE RESEARCH
(2021)
Article
Microbiology
Lushan Zhang, Liang Ma, Qiuhong Yang, Yongtao Liu, Xiaohui Ai, Jing Dong
Summary: The study aimed to find alternative treatments to antibiotics. Through disrupting quorum sensing, sanguinarine was found to inhibit the growth and biofilm formation of A. hydrophila. The results showed that sanguinarine protected A549 cells and reduced mortality in infected channel catfish.
Article
Biochemistry & Molecular Biology
Bruno Oliveira Silva Duran, Bruna Tereza Thomazini Zanella, Erika Stefani Perez, Edson Assuncao Mareco, Josefina Blasco, Maeli Dal-Pai-Silva, Daniel Garcia de la Serrana
Summary: Amino acids and IGF1 play different roles in regulating gene expression in pacu-cultured myotubes, with potential molecular markers identified. Muscle miRNAs were up-regulated especially in AA samples, revealing various potential molecular networks through omics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Zhen Li, Lishan Zhang, Qingli Song, Guibin Wang, Wenxiao Yang, Huamei Tang, Ramanathan Srinivasan, Ling Lin, Xiangmin Lin
Summary: This study examined the role of transcriptional regulators in enoxacin resistance in Aeromonas hydrophila and found that deletion of ahslyA up-regulated key antibiotic resistance proteins under ENX stress, reducing pressure by preventing activation of the SOS repair system. The research also revealed a complex regulatory network involving at least 11 TRs induced by ahslyA, and identified specific genes that may contribute to ENX resistance. Overall, the data demonstrated the significant role of ahslyA in ENX resistance and provided new insights into transcriptional regulation effects on antibiotic resistance in bacteria.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Fisheries
Fernando Carlos Ramos-Espinoza, Victor Alexander Cueva-Quiroz, Jefferson Yunis-Aguinaga, Julieta Rodini Engracia de Moraes
Article
Food Science & Technology
Daniel Leonardo Cala Delgado, Caled Alvarez Rubio, Victor Alexander Cueva Quiroz
Summary: Feeding accounts for a significant portion of production costs in fish farming, prompting the use of alternative raw materials. This study conducted analyses on red tilapia fed with biofloc meal, revealing it to be a sustainable alternative with satisfactory sensory and microbiological results.
FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Fisheries
Andria Gama Sousa, Acacio H. B. Pacheco, Gilson A. Siqueira-Pinto, Gleika T. J. dos Reis, Michelle M. S. Fugimura, Luciano J. Vaz, Paulo F. Marcusso, Fernando C. Ramos-Espinoza, Gustavo da Silva Claudiano
Summary: This study evaluated the effects of benzocaine and eugenol on anesthesia in tambaqui fish, as well as the use of different anticoagulants on hematological and biochemical parameters. Results showed that eugenol-induced anesthesia had better performance compared to benzocaine, with faster induction and recovery times and fewer alterations in blood parameters. Additionally, the use of EDTA was found to facilitate erythrocyte conservation and cause fewer blood alterations compared to other anticoagulants.
AQUACULTURE INTERNATIONAL
(2021)
Article
Fisheries
Luiz F. A. Costa, Jhonatan S. Lima, Alicia M. A. Siqueira, Elissandro C. C. da Silva, Vivian C. F. Lima, Lucas A. da Silva, Suzete R. da Silva, Thalis F. Santos, Michelle Midori Sena Fugimura, Luciano Jensen Vaz, Paulo Fernandes Marcusso, Gustavo da Silva Claudiano
Summary: The use of probiotics during tambaqui breeding was proven to be safe, but adding multi-species probiotics to water or feed did not provide additional benefits in terms of zootechnical performance, water quality, and tambaqui health under the applied experimental conditions.
AQUACULTURE NUTRITION
(2021)
Article
Fisheries
Paulo Fernandes Marcusso, Gustavo da Silva Claudiano, Jefferson Yunis-Aguinaga, Fausto de Almeida Marinho-Neto, Silas Fernandes Eto, Dayanne Carla Fernandes, Mayara Caroline Rosolem, Rogerio Salvador, Julieta Rodini Engracia de Moraes, Flavio Ruas de Moraes
Summary: This study focused on finding a more effective and safer antigenic fraction to develop a vaccine against streptococcosis in Nile tilapia. The insoluble fraction vaccine showed the best protective effect with a 100% relative percent of survival. The spleen may play a key role in the vaccine response in Nile tilapia.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Isabela F. S. Perossi, Paulo E. B. Martinelli, Marita Vedovelli Cardoso, Julieta R. E. de Moraes
Summary: This study aimed to identify and analyze the antibacterial susceptibility of bacteria in a veterinary autopsy room, and to test the effectiveness of two sanitizers against these microorganisms. The results indicated that hypochlorite was the most effective sanitizing agent and highlighted the importance of alerting professionals to the biological risks and precautions they should take.
REVISTA MVZ CORDOBA
(2022)
Article
Fisheries
Dayanne Carla Fernandes, Silas Fernandes Eto, Gustavo da Silva Claudiano, Paulo Fernandes Marcusso, Fernando Carlos Ramos-Espinoza, Julieta Rodini Engracia de Moraes
Summary: This study aimed to obtain purified polyclonal immunoglobulin Y (IgY) for the diagnosis of A. hydrophila by immunizing chickens and demonstrated the utility of IgY in detecting A. hydrophila within the phagosomes of phagocytes and extracellular bacteria colonizing host tissues in pacus.
AQUACULTURE RESEARCH
(2022)
Article
Fisheries
Elizabeth Gusmao Affonso, Marieta Nascimento de Queiroz, Eduardo Akifumi Ono, Sanny Maria de Andrade Porto, Gustavo da Silva Claudiano
Summary: The aim of this study was to assess the use of sodium chloride against monogenoid trematodes infestations in pirarucu. The results showed that sodium chloride had a good anthelmintic effect on the parasites at certain concentrations, but it also had some physiological effects on the fish. Furthermore, all the parasites found in the gills were identified as a specific type of monogenoid.
AQUACULTURE INTERNATIONAL
(2023)
Article
Fisheries
Raphael Brito dos Santos, Juda Izel-Silva, Paulo Adelino de Medeiros, Michelle Midori Sena Fugimura, Thiago Mendes de Freitas, Eduardo Akifumi Ono, Gustavo da Silva Claudiano, Elizabeth Gusmao Affonso
Summary: This study compared the effects of different feed crude protein levels in biofloc vs. clear water systems on the growth performance, digestive enzymes, and body composition of tambaqui juveniles. The results showed that tambaqui reared in the biofloc system exhibited better performance indices, such as feed conversion and protein efficiency rates, as well as higher trypsin activity. However, no significant differences were found in tambaqui proximal composition and other digestive enzymes. These findings suggest that a feed containing 24% crude protein can be offered to tambaqui in both systems.
AQUACULTURE INTERNATIONAL
(2023)
Article
Fisheries
Carla Fernandez-Espinel, Marco Medina-Morillo, Rute Irgang, Giovanna Sotil, Henry Araya-Leon, Violeta Flores-Dominick, Jesus L. Romalde, Ruben Avendano-Herrera, Jefferson Yunis-Aguinaga
Summary: This study aimed to identify and classify Yersinia ruckeri isolates from the Puno Region in Peru. Two biotypes were found among the isolates, but they shared identical fingerprints and serotypes. This information is important for the epidemiological research and vaccine development against Y. ruckeri.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Jose Lima Moraes, Sanny Maria de Andrade-Porto, Eduardo Akifumi Ono, Gustavo Da Silva Claudiano, Elizabeth Gusmao Affonso
Summary: This study evaluated the efficacy of therapeutic levamisole hydrochloride (LVC) baths against the endoparasite Neoechynorhyncus buttnerae and its effects on juvenile tambaqui blood parameters. The results showed that LVC was highly effective in both in vitro and in vivo tests, without compromising tambaqui juvenile homeostasis.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Raphael Brito dos Santos, Juda Izel-Silva, Paulo Adelino de Medeiros, Michelle Midori Sena Fugimura, Thiago Mendes de Freitas, Silvia Umeda Gallani, Gustavo da Silva Claudiano, Eduardo Akifumi Ono, Elizabeth Gusmao Affonso
Summary: The present study evaluated the immunophysiological response in Colossoma macropomum fed with different levels of dietary protein in a biofloc (BFT) and in clear-water (CW) system and under infection with Aeromonas jandaei. The results showed that there were no significant differences in the physiological conditions of the fish between the different rearing systems and protein levels. However, fish raised in the biofloc system showed improved immunological resistance and higher survival rates after bacterial infection.
AQUACULTURE INTERNATIONAL
(2023)
Article
Fisheries
Fernando Carlos Ramos-Espinoza, Victor Alexander Cueva-Quiroz, Norquis Caled Alvarez-Rubio, Nicoli Paganoti de Mello, Julieta Rodini Engracia de Moraes
Summary: This study aimed to investigate the effect of dietary MOS supplementation on immune responses in fish after vaccination with a novel vaccine. The results showed that adding MOS after vaccination did not significantly enhance vaccine efficacy, which could be attributed to the high efficacy of the vaccine itself.
JOURNAL OF THE WORLD AQUACULTURE SOCIETY
(2023)