Article
Biochemistry & Molecular Biology
Ved Prakash, Chad T. Nihranz, Clare L. Casteel
Summary: Virus infection can increase drought tolerance in plants, but the underlying mechanisms are not well understood. In this study, we found that infection with turnip mosaic virus (TuMV) enhances the survival of Arabidopsis thaliana under drought conditions. By expressing each of the major viral proteins in transgenic A. thaliana, we identified three proteins, 6K1, 6K2, and NIa-Pro, that confer drought tolerance. Specifically, the expression of 6K2 leads to increased levels of salicylic acid (SA), which is associated with the induction of secondary metabolites, osmolytes, and antioxidants that enhance drought tolerance.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2023)
Article
Microbiology
Esther O. Bello, Yingshuai Yang, Yue Fang, Mengzhu Chai, Xue Jiang, Yameng Luan, Yuting Wang, Yating Guo, Xiao-Yun Wu, Xiaofei Cheng, Xiao-Xia Wu
Summary: The P1 protein in Potyvirus is crucial for viral infection and adaptation, although its exact mechanism is not fully understood. Through yeast-two-hybrid screening, eight potential P1-interacting proteins in Arabidopsis were identified, with NODULIN 19 (NOD19) being selected for further investigation. Further experiments confirmed the interaction between TuMV P1 and NOD19, which is a membrane-associated protein expressed mainly in plant aerial parts. Knockdown of NOD19 in Arabidopsis and soybean resulted in attenuated infection of TuMV and soybean mosaic virus, respectively. These findings suggest that NOD19 is a host factor required for robust viral infection.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Virology
Kaida Ding, Zhaoxing Jia, Penghuan Rui, Xinxin Fang, Hongying Zheng, Jianping Chen, Fei Yan, Guanwei Wu
Summary: The study investigates the role of host factors and cellular protein changes in the early stages of Turnip mosaic virus (TuMV) infection in Nicotiana benthamiana plants. The researchers identified 225 differentially accumulated proteins (DAPs), including four upregulated proteins belonging to the UGT family, that influenced TuMV replication and reactive oxygen species production. This comparative proteomics analysis provides new insights into the mechanisms of plant viral infection.
Article
Plant Sciences
Long He, Peng Jin, Xuan Chen, Tian-Ye Zhang, Kai-Li Zhong, Peng Liu, Jian-Ping Chen, Jian Yang
Summary: The study identified changes in host proteins during CWMV infection, with a focus on disruptions in chloroplast functions and decreased ABA concentrations. The suppression of the ABA pathway during CWMV infection and the induction of host defenses against CWMV through ABA treatment were highlighted.
Article
Plant Sciences
Kristin Widyasari, John Bwalya, Kook-Hyung Kim
Summary: Infection of Nicotiana benthamiana by viruses from the genera Bromovirus, Potyvirus, and Potexvirus induces up-regulation of genes encoding the HSP70 family, including BiP2. Knockdown of three up-regulated genes showed that Bip2 is important for potyvirus infection but not for the other tested viruses. Knockdown of BiP2 resulted in reduced accumulation of SMV and PepMoV RNA, and it was found to interact with SMV's NIb protein. Therefore, BiP2 plays a crucial role as a proviral host factor in potyvirus infection.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Virology
Haritha Nunna, Feng Qu, Satyanarayana Tatineni
Summary: This study reveals that the P3 and NIa-Pro proteins encoded by Turnip mosaic virus (TuMV) can induce superinfection exclusion (SIE), preventing the replication of TuMV-GFP. Specific regions of these two proteins play essential roles in SIE activity.
Article
Plant Sciences
Tsuyoshi Inukai, Hangil Kim, Wataru Matsunaga, Chikara Masuta
Summary: It has been found that TuMV suppresses AA in Brassica rapa leaves, but leaves with high AA are rarely infected. AA serves as a chemical barrier against TuMV. TuMV also inhibits AA induced by high light in Arabidopsis by suppressing the expression of anthocyanin late-biosynthesis genes.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Microbiology
Shiyou Qiu, Xuwei Chen, Yushan Zhai, Weijun Cui, Xuhong Ai, Shaofei Rao, Jianping Chen, Fei Yan
Summary: The LHCB3 protein in plants plays a crucial role in defense against TuMV infection by inducing the production of ROS, which enhances the resistance of plants to the virus.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kairi Karblane, Andrew E. Firth, Allan Olspert
Summary: This study investigates the dynamics of transcriptional slippage in Potyviruses, finding a modest increase in insertion of viral transcripts at later timepoints during infection. Differences in insertional slippage between different RNA subpopulations are observed, and there may be selection against the use of (-)RNAs with insertions as templates for transcription or replication.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Article
Plant Sciences
Hsiang-Chi Lee, Ying-Ping Huang, Ying-Wen Huang, Chung-Chi Hu, Chin-Wei Lee, Chih-Hao Chang, Na-Sheng Lin, Yau-Heiu Hsu
Summary: This study reveals the involvement of mitochondrial voltage-dependent anion channel proteins in the dynamic Bamboo mosaic virus (BaMV)-induced complex, which supports BaMV accumulation in Nicotiana benthamiana. The study also demonstrates that interaction with BaMV triple gene block protein 1 (TGBp1) can induce aggregation of the NbVDAC proteins and promote BaMV replication. These findings highlight the importance of mitochondrial proteins in viral complex formation and virus infection.
Article
Plant Sciences
Xuefeng Li, Lixue Xie, Xihong Chen, Jiangshan Chen, Jianguo Shen, Fangluan Gao
Summary: Contigs similar to turnip mosaic virus (TuMV) were identified in a diseased passion fruit plant in Fujian Province, China, through deep sequencing of extracted small RNAs. The complete genome of TuMV, named TuMV-FJ, was determined based on these contigs and Sanger sequencing. The TuMV-FJ genome is 9,833 nucleotides in length and contains an open reading frame encoding a polypeptide with 3164 amino acid residues. TuMV-FJ belongs to a world-B phylogenetic group and shares over 94% sequence identity with its closest relative, BJ-B01. An RT-LAMP assay was developed for rapid detection of TuMV-FJ. This study reports the first complete genome sequence of TuMV infecting P. edulis and provides a sensitive detection method using RT-LAMP.
JOURNAL OF PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Guillermo Baena, Ana B. Feria, Luis Hernandez-Huertas, Jacinto Gandullo, Cristina Echevarria, Jose A. Monreal, Sofia Garcia-Maurino
Summary: Phosphoenolpyruvate carboxylase (PEPC) is involved in carbon and nitrogen metabolisms, and a portion of it undergoes degradation via selective autophagy, with monoubiquitination playing a key role in the process. These findings increase understanding of the regulation of PEPC degradation.
Article
Virology
Shusuke Kawakubo, Yasuhiro Tomitaka, Kenta Tomimura, Ryoko Koga, Hiroki Matsuoka, Seiji Uematsu, Kazuo Yamashita, Simon Y. W. Ho, Kazusato Ohshima
Summary: The study found a large number of recombination events in the genome of Turnip Mosaic Virus (TuMV) in Japan, and the virus spread to different parts of Japan before the 20th century. This study also emphasizes the importance of recombination events in the genome and provides an example of virus epidemiology surveying.
Article
Biochemistry & Molecular Biology
Bernardo Villegas-Estrada, Manuel Alejandro Sanchez, Arnubio Valencia-Jimenez
Summary: This study successfully synthesized virus-derived artificial small hairpin RNAs (shRNAs) using a dsDNA template and triggered the RNAi mechanism in Nicotiana benthamiana plants against CMV. The gene silencing was confirmed using PCR, immunological-based assays, and qPCR.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Tong Zhu, Xue Zhou, Jian-Long Zhang, Wei-Hao Zhang, Li-Pei Zhang, Chun-Xiang You, Paula E. Jameson, Peng-Tao Ma, Shan-Li Guo
Summary: Several MYB transcription factors, including MYB4-like, play vital roles in plant resistance to environmental stressors. The study revealed that NbMYB4L is involved in defense against TMV in Nicotiana benthamiana, with the ethylene pathway also playing a role. NbEIL1 acts as a positive regulator of NbMYB4L transcription in the plant, forming a coherent feed-forward loop that robustly regulates resistance to TMV.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Mingzhen Zhang, Pan Gong, Linhao Ge, Zhaoyang Chang, Xiaofei Cheng, Xueping Zhou, Aiming Wang, Fangfang Li
Summary: The study reveals that XPO1 plays a crucial role in TuMV infection by interacting with NIb to facilitate its translocation to viral replication complexes, as well as mediating the nuclear export of sumoylated NIb and host factors tagged with SUMO3 to suppress plant immunity. Deficiencies of XPO1 in Arabidopsis and Nicotiana benthamiana plants inhibit TuMV replication and infection, highlighting the importance of XPO1 as a host factor in potyvirus infections.
Article
Plant Sciences
Yushan Zhai, Quan Yuan, Shiyou Qiu, Saisai Li, Miaomiao Li, Hongying Zheng, Guanwei Wu, Yuwen Lu, Jiejun Peng, Shaofei Rao, Jianping Chen, Fei Yan
Summary: Chloroplast NADH dehydrogenase-like complex M subunit gene (NdhM) plays a crucial role in plant defense against viral infection by regulating chloroplast clustering to resist virus invasion.
PLANT CELL AND ENVIRONMENT
(2021)
Editorial Material
Plant Sciences
A. Simkovich, S. E. Kohalmi, A. Wang
Article
Virology
Aaron J. Simkovich, Yinzi Li, Susanne E. Kohalmi, Jonathan S. Griffiths, Aiming Wang
Summary: This study identified PDV from sweet cherry in the Niagara fruit belt of Ontario, Canada, constructed an infectious cDNA clone inducing dwarfing phenotype in cherry, and found that the clone was infectious on cucurbits and Nicotiana benthamiana but not on Arabidopsis thaliana.
Article
Microbiology
Xiangxiang Zhang, Yueyan Yin, Yunhe Su, Zhaoxing Jia, Liangliang Jiang, Yuwen Lu, Hongying Zheng, Jiejun Peng, Shaofei Rao, Guanwei Wu, Jianping Chen, Fei Yan
Summary: eIF4A acts as a negative regulator of autophagy in plants and plays a crucial role in antiviral defense mechanisms.
Article
Microbiology
Guanwei Wu, Zhaoxing Jia, Kaida Ding, Hongying Zheng, Yuwen Lu, Lin Lin, Jiejun Peng, Shaofei Rao, Aiming Wang, Jianping Chen, Fei Yan
Summary: Experimental evidence has shown that TuMV virus replicates through late endosome, and the VSR4 protein in Arabidopsis plays a crucial role in targeting viral replication vesicles to the enlarged endosome. N-glycosylation of VSR4 is required for its stability and monitoring the trafficking of viral replication vesicles to the enlarged endosome.
Article
Microbiology
Mengfei Ji, Jinping Zhao, Kelei Han, Weijun Cui, Xinyang Wu, Binghua Chen, Yuwen Lu, Jiejun Peng, Hongying Zheng, Shaofei Rao, Guanwei Wu, Jianping Chen, Fei Yan
Summary: Viruses counteract plant antiviral immunity by interfering with the crucial hormone jasmonic acid biosynthesis and signaling pathway through interaction with a conserved chloroplast protein, cpSRP54. This mechanism, involving suppression of cpSRP54 function, may be a common strategy used by viruses to enhance viral infection by suppressing jasmonic acid biosynthesis.
Article
Plant Sciences
Shaokang Zhang, Jonathan S. Griffiths, Genevieve Marchand, Mark A. Bernards, Aiming Wang
Summary: Tomato brown rugose fruit virus (ToBRFV) is an emerging RNA virus that infects tomato and pepper and threatens tomato production worldwide. It is mainly transmitted through contaminated seeds and mechanical contact, and can break down genetic resistance. Currently, no ToBRFV-resistant tomato cultivars are available, and traditional management measures have limited success. The development of novel genetic resistance and resistant cultivars is the most effective strategy for controlling ToBRFV.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Guanwei Wu, Zhaoxing Jia, Penghuan Rui, Hongying Zheng, Yuwen Lu, Lin Lin, Jiejun Peng, Shaofei Rao, Aiming Wang, Jianping Chen, Fei Yan
Summary: This study identified the importance of acidic dileucine motifs in the CI protein of Turnip mosaic virus for interaction with AP2 beta, which is crucial for efficient viral replication.
MOLECULAR PLANT PATHOLOGY
(2022)
Review
Virology
Mariola Usovsky, Pengyin Chen, Dexiao Li, Aiming Wang, Ainong Shi, Cuiming Zheng, Ehsan Shakiba, Dongho Lee, Caio Canella Vieira, Yi Chen Lee, Chengjun Wu, Innan Cervantez, Dekun Dong
Summary: This review summarizes the history and current state of the genetic basis for soybean resistance to Soybean mosaic virus (SMV) and the application of molecular markers in crop improvement. It discusses the molecular underpinnings of SMV-soybean interactions and the distribution and interactions of resistance genes. Although the physical position of some resistance genes has been identified, there are still elusive causative genes. Soybean resistance to SMV has been achieved through the combination of multiple genes.
Article
Cell Biology
Qifu Liang, Jiajia Wan, Huan Liu, Dongsheng Jia, Qian Chen, Aiming Wang, Taiyun Wei
Summary: Southern rice black-streaked dwarf virus (SRBSDV) can induce mitophagy in its vector to prevent apoptosis and promote viral propagation. The nonstructural protein P7-1 of SRBSDV targets mitochondria and interacts with the mitophagy receptor BNIP3 to initiate mitophagy. This study reveals the important role of virus-induced mitophagy in persistent viral infection.
Review
Virology
Rongrong He, Yinzi Li, Mark A. Bernards, Aiming Wang
Summary: Viruses infect all cellular life forms and cause various diseases and significant economic losses worldwide. They induce altered membrane structures in infected host cells, forming viral replication organelles or complexes. These organelles provide an optimum environment for robust viral replication and can also be mobile, reaching neighboring cells via plasmodesmata through the endomembrane-cytoskeleton network.
Article
Virology
Eunseo Lee, Raj Vansia, James Phelan, Andrea Lofano, Adam Smith, Aiming Wang, Guillaume J. Bilodeau, Stephen F. Pernal, M. Marta Guarna, Michael Rott, Jonathan S. Griffiths
Summary: Healthy agroecosystems rely on a complex web of factors and interactions. Bees can transmit plant-viruses and other pathogens while pollinating flowers, which is critical for fruit production. A metagenomics-based monitoring system identified 29 unique plant viral species in blueberry farms in British Columbia and 9 viruses in Ontario. Bee-mediated pathogen monitoring provides insights into virus diversity and ecosystem health, although it may be limited by sampling season and diverse plant material collected by bees.
Article
Multidisciplinary Sciences
Jiahui Liu, Xiaoyun Wu, Yue Fang, Ye Liu, Esther Oreofe Bello, Yong Li, Ruyi Xiong, Yinzi Li, Zheng Qing Fu, Aiming Wang, Xiaofei Cheng
Summary: NONEXPRESSER OF PATHOGENESIS-RELATED GENES 1 (NPR1) is the master regulator of salicylic acid-mediated basal and systemic acquired resistance in plants. The viral RNA-dependent RNA polymerase NUCLEAR INCLUSION B (NIb) counteracts the resistance by binding to NPR1 and preventing its sumoylation. This interaction also hinders the phosphorylation of NPR1, leading to the suppression of NPR1-mediated immunity.
NATURE COMMUNICATIONS
(2023)