期刊
PHYTOCHEMICAL ANALYSIS
卷 31, 期 1, 页码 68-80出版社
WILEY
DOI: 10.1002/pca.2867
关键词
dietary supplement; metabolomics; natural health product; nuclear magnetic resonance (NMR); Vaccinium macrocarpon
资金
- Leo and Anne Albert Charitable Trust
- UMass Cranberry Health Research Center
- NIST
- Cranberry Institute
Introduction Commercial cranberry supplements provide a low-sugar alternative to juices and sweetened fruit consumed for health benefits, but their phytochemical composition and associated biological activity varies depending on the source material and post-harvest processing. Proton nuclear magnetic resonance (H-1-NMR) is a rapid and environmentally friendly method of generating metabolic profiles of plant materials that may be used to authenticate cranberry products. Objective The H-1 NMR-based chemometrics were used to characterise variations in metabolic profiles of cranberry supplements in comparison to a whole cranberry powder reference standard. Materials and Methods The secondary metabolite profiles of nine commercial cranberry supplements were compared to a whole cranberry powder reference standard, using H-1-NMR with Bruker AssureNMR software and principal component analysis (PCA). Content of selected triterpenoids and organic acids was determined by quantitative NMR. Total proanthocyanidins and anthocyanins were determined by established methods. Results PCA of H-1-NMR spectra showed overlap between the cranberry standard and three supplements, but most products varied substantially in metabolic profile. Metabolites contributing to the observed variance include citric acid and cranberry peel constituents ursolic acid, oleanolic acid and hyperoside. Ursolic, oleanolic, citric, quinic and malic acids were readily determined by quantitative H-1-NMR in the whole cranberry standard, but were below detection limits in many supplements. Proanthocyanidin and flavonoid content in several products was minimal or below detection limits. Conclusion The H-1-NMR chemometrics found significant variation in composition of characteristic cranberry metabolites among commercial preparations, reinforcing the need for reliable industry standards
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据