Article
Microbiology
Holly K. Huse, Mark J. Lee, Mandy Wootton, Susan E. Sharp, Maria Traczewski, John J. LiPuma, Peter Jorth
Summary: This study evaluated the reproducibility and accuracy of different antimicrobial susceptibility testing methods for Burkholderia cepacia complex (BCC). The findings showed poor accuracy for disk diffusion and MicroScan WalkAway methods, while broth microdilution method demonstrated higher reproducibility for meropenem and minocycline.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shady Estfanous, Kathrin Krause, Midhun N. K. Anne, Mostafa Eltobgy, Kyle Caution, Arwa Abu Khweek, Kaitlin Hamilton, Asmaa Badr, Kylene Daily, Cierra Carafice, Daniel Baetzhold, Xiaoli Zhang, Tianliang Li, Haitao Wen, Mikhail A. Gavrilin, Hesham Haffez, Sameh Soror, Amal O. Amer
Summary: The study revealed that GSDMD restricts bacterial replication, stimulates autophagy, and promotes inflammation by mediating the release of inflammasome dependent and independent cytokines in response to B. cenocepacia infection.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Asmaa Badr, Mostafa Eltobgy, Kathrin Krause, Kaitlin Hamilton, Shady Estfanous, Kylene P. Daily, Arwa Abu Khweek, Ahmad Hegazi, Midhun N. K. Anne, Cierra Carafice, Frank Robledo-Avila, Youssra Saqr, Xiaoli Zhang, Tracey L. Bonfield, Mikhail A. Gavrilin, Santiago Partida-Sanchez, Stephanie Seveau, Estelle Cormet-Boyaka, Amal O. Amer
Summary: Cystic fibrosis (CF) macrophages show defects in clearing bacteria and autophagy process. CFTR is recruited to autophagosomes and improves autophagy flux, lysosomal acidification, function, and bacterial clearance. These findings clarify the role of CFTR in maintaining cellular acidification and autophagy process.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Olga Kondratenko, Artem Lyamin, Tatiana Savinova, Yuliya Bocharova, Elena Vasilyeva, Igor Chebotar
Summary: This report describes a pediatric case of paranasal sinusitis caused by Burkholderia cenocepacia in a cystic fibrosis patient. It is an unusual case where the paranasal sinuses were the only site of colonization by B. cenocepacia for five years, with no infection in other organs. The sinuses were sanitized by endoscopic sinus surgery and no B. cenocepacia was detected in samples after the surgery, even without systemic antibiotic therapy for two years. This case demonstrates the possibility of prolonged remission of Bcc-associated paranasal sinusitis in the absence of systemic antibiotics.
Article
Microbiology
Kevin R. Barker, Michael Santino, John J. LiPuma, Elizabeth Tullis, Matthew P. Muller, Larissa M. Matukas, Manal Tadros
Summary: The IR Biotyper and MALDI-TOF MS-ClinProTools offer rapid and accurate typing of bacteria, with diagnostic sensitivity exceeding 80% for detecting the epidemic strain ET12. However, both methods showed suboptimal accuracy for typing outbreak strains of B. cenocepacia, which could potentially be improved by extending the spectral region analyzed by the IR Biotyper.
MICROBIOLOGY SPECTRUM
(2021)
Article
Immunology
Edgard de Freitas Vianna, Nicoli Izzy Miotto Menezes, Mila Muraro de Almeida, Fla'via Alvim Dutra de Freitas, Cassiana da Costa Ferreira Leite, Monica Cassia Firmida, Davi Ribeiro Iglesias de Medeiros, Robson Souza Leao, Rodolpho Mattos Albano, Elizabeth Andrade Marques
Summary: The genome analysis of Burkholderia cenocepacia complex III revealed high virulence and antimicrobial resistance, with a predominance of genes related to invasion mechanisms and flagellar biosynthesis. Additionally, the isolate exhibited bacterial dissemination mechanisms with invasive determinants.
Review
Biochemistry & Molecular Biology
Evelina Moliteo, Monica Sciacca, Antonino Palmeri, Maria Papale, Sara Manti, Giuseppe Fabio Parisi, Salvatore Leonardi
Summary: There is substantial evidence that patients with cystic fibrosis (CF) have higher oxidative stress levels, which contribute to the progression of chronic lung damage. CF patients exhibit an abnormal proinflammatory environment in their airways even before infection, possibly due to elevated oxidative stress and abnormal lipid metabolism. CFTR deficiency appears to cause a redox imbalance in epithelial cells and extracellular fluids.
Article
Biochemistry & Molecular Biology
Aaron T. Butt, Christopher D. Banyard, Sayali S. Haldipurkar, Kirsty Agnoli, Muslim Mohsin, Srdjan Vitovski, Ameya Paleja, Yingzhi Tang, Rebecca Lomax, Fuzhou Ye, Jeffrey Green, Mark S. Thomas
Summary: This study uncovers the mechanism by which Burkholderia cenocepacia bacteria sense intracellular iron concentration. In addition to the regulation by the ferric uptake regulator, the internal iron-sensing protein of the bacteria also responds to iron concentration.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Respiratory System
C. Olcese, R. Casciaro, D. Pirlo, C. Debbia, E. Castagnola, F. Cresta, C. Castellani
Summary: In this case, a CF patient was co-infected with COVID-19 and Bc, and was treated with antibiotics, dexamethasone, remdesivir, and heparin, leading to gradual improvement and discharge. The reciprocal role of SARS-CoV-2 and Bc, as well as the impact of individual therapies on the outcome, remain unclear.
JOURNAL OF CYSTIC FIBROSIS
(2021)
Article
Chemistry, Applied
Barbara Bellich, Lucrecia C. Teran, Magnus M. Fazli, Francesco Berti, Roberto Rizzo, Tim Tolker-Nielsen, Paola Cescutti
Summary: Burkholderia cenocepacia is an opportunistic pathogen that causes in-fections in cystic fibrosis patients. Biofilm and its associated exopolysaccharides play a crucial role in infection persistence and antibiotic tolerance. This study identified the water-insoluble exopolysaccharide encoded by the B. cenocepacia H111 bepA-L gene cluster and highlighted its potential importance in in vivo infections.
CARBOHYDRATE POLYMERS
(2023)
Review
Biochemistry & Molecular Biology
Caitlyn Harvey, Sinead Weldon, Stuart Elborn, Damian G. Downey, Clifford Taggart
Summary: The advent of CFTR modulators in cystic fibrosis treatment has transformed the management of the disease, shifting it from being a life-limiting condition to one that can be effectively managed. These genotype-specific therapies have shown significant improvements in various clinical endpoints, but their effects on pathogenic burden and airway infection need further exploration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Michael G. Dorrington, Clinton J. Bradfield, Justin B. Lack, Bin Lin, Jonathan J. Liang, Tregei Starr, Orna Ernst, Julia L. Gross, Jing Sun, Alexandra H. Miller, Olivia Steele-Mortimer, Iain D. C. Fraser
Summary: This study focuses on how macrophages combat opportunistic bacterial pathogen Burkholderia cenocepacia and highlights the crucial role of interferons in killing these bacteria. The results could help guide anti-Burkholderia treatments in susceptible patients. The study emphasizes the importance of type I interferons in protection against opportunistic pathogens through innate immunity, without the need for damaging inflammatory responses.
Article
Infectious Diseases
Aditi Aiyer, Arthika Manoharan, Denis Paino, Jessica Farrell, Gregory S. Whiteley, Frederik H. Kriel, Trevor O. Glasbey, Jim Manos, Theerthankar Das
Summary: Cystic fibrosis is a genetic disease that results in thick lung mucus which can be colonised by bacteria, leading to biofilm formation. This study found that a combination therapy of NAC and ciprofloxacin can significantly disrupt B. cenocepacia biofilms and reduce the necessity for high concentrations of antibiotics.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Pharmacology & Pharmacy
Jia Liu, Allison P. Berg, Yiting Wang, Walailak Jantarajit, Katy J. Sutcliffe, Edward B. Stevens, Lishuang Cao, Marko J. Pregel, David N. Sheppard
Summary: This study investigates the action of a new CFTR potentiator, CP-628006, and compares it with the marketed CFTR potentiator ivacaftor. CP-628006 has distinct effects compared to ivacaftor, suggesting a different mechanism of CFTR potentiation. The emergence of CFTR potentiators with diverse modes of action makes therapy with combinations of potentiators a possibility.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Medicine, General & Internal
Hannah Farley, Sarah Poole, Stephen Chapman, William Flight
Summary: A retrospective single-center cohort study identified 19 adult patients diagnosed with CF, all of whom had a history of chronic respiratory symptoms and the majority of whom had a CFTR genotype considered eligible for CFTR modulator therapy.
POSTGRADUATE MEDICAL JOURNAL
(2022)
Article
Immunology
Hany Khalil, Amira Abd ElHady, Khaled A. Elawdan, Dalia Mohamed, Doaa D. Mohamed, Ahmed I. Abd El Maksoud, Farha A. El-Chennawi, Bhgat EI-Fikiy, Ibrahim H. El-Sayed
Summary: Autophagy is a cellular process that maintains cell survival and energy sources, and is associated with the development of many human diseases. Controlling autophagy levels can effectively combat these diseases and the ongoing coronavirus epidemic.
IMMUNOLOGICAL INVESTIGATIONS
(2022)
Article
Behavioral Sciences
Divine C. Nwafor, Sreeparna Chakraborty, Sujung Jun, Allison L. Brichacek, Margaret Dransfeld, Darren E. Gemoets, Duaa Dakhlallah, Candice M. Brown
Summary: Alzheimer's Disease (AD) is the most prevalent form of dementia globally, and studies have shown that AD neuropathology accompanies alterations in learning and memory. This study revealed that CVN-AD mice exhibit impaired metabolic activity, sleep, and sensorimotor function, resembling human-AD clinical progression.
BEHAVIOURAL BRAIN RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Qing Ye, Rehab Mohamed, Duaa Dakhlallah, Marieta Gencheva, Gangqing Hu, Martin C. Pearce, Siva Kumar Kolluri, Clay B. Marsh, Timothy D. Eubank, Alexey V. Ivanov, Nancy Lan Guo
Summary: This study demonstrated that high expression of ZNF71 KRAB is associated with poor survival outcomes in NSCLC patients, as well as with epithelial-to-mesenchymal transition and chemotherapy resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Energy & Fuels
Abdelaziz L. Khlaifat, Duaa Dakhlallah, Faraz Sufyan
Summary: This article discusses the importance of alkaline flooding and its features compared to other flooding methods, including alkaline flooding, hybrid modes of injection, experimental work, pilot projects, screening criteria, and field applications. The findings of this study can help increase the understanding of alkaline flooding and provide a holistic view of the hybrid modes of flooding.
Review
Biochemistry & Molecular Biology
Devi Jaganathan, Emanuela M. Bruscia, Benjamin T. Kopp
Summary: Cystic fibrosis is a disease caused by mutations in the CFTR gene, resulting in chronic inflammation and lung function decline. Macrophages play a crucial role in CF and the mutant CFTR affects phagocytosis, which is linked to disease progression. A better understanding of the interaction between CFTR and macrophages during phagosome formation could contribute to the development of new therapeutic strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pediatrics
Sabrina Palacios, Katelyn Krivchenia, Mariah Eisner, Bailey Young, Octavio Ramilo, Asuncion Mejias, Simon Lee, Benjamin T. Kopp
Summary: This study is the largest to date on long-term pulmonary sequelae in pediatric patients after COVID-19. Identified clinical phenotypes and risk factors require further study and treatment.
PEDIATRIC PULMONOLOGY
(2022)
Article
Medicine, Research & Experimental
Khaled A. Elawdan, Sabah Farouk, Salah Aref, Hamada Shoaib, Mohamed A. El-Razik, Nasser H. Abbas, Mohamed Younis, Abeer A. Alshambky, Hany Khalil
Summary: The study found that cancer patients with low levels of vitamin B12 and ferritin showed changes in the expression of specific genes and progression of DNA methylation.
BIOMARKERS IN MEDICINE
(2022)
Article
Microbiology
Nasser H. Abbas, Afaf Elsayed, Hamdy A. Hassan, Sabha El-Sabbagh, Ashraf F. Elbaz, Hany Khalil
Summary: In this study, new isolates of Halomonas and Marinobacter were obtained from Hamralake in Egypt, which can degrade phenol under high salt and pH conditions via two different mechanisms.
CURRENT MICROBIOLOGY
(2022)
Letter
Pediatrics
Eric S. Mull, Sarah Cohen, Ashish George, Katelyn Krivchenia, Stephen Druhan, Peter B. Baker, Benjamin Kopp
PEDIATRIC PULMONOLOGY
(2023)
Article
Hematology
Rachel N. Zeno, Joseph Stanek, Courtney Pugh, Michelle Gillespie, Benjamin T. Kopp, Susan Creary
Summary: An interdisciplinary clinic providing pulmonary care for people with sickle cell disease (pwSCD) was created in 2014 to address health care access barriers. The study found that pwSCD had significantly fewer hospitalizations for acute chest syndrome, asthma, and vaso-occlusive episodes in the 2 years after their initial SCD-pulmonary clinic visit compared with the 2 years before. This suggests that implementing a multidisciplinary SCD-pulmonary clinic can improve patient management and usage of acute care.
Article
Microbiology
Sherry A. Guirgis, Khalil A. El-Halfawy, Mai Alalem, Hany Khalil
Summary: This study investigates DNA methylation changes in human lung epithelial cells during L. pneumophila infection and the inhibitory effects of methylation inhibitor agents on L. pneumophila replication. The results showed that L. pneumophila infection induces methylation activity in the promoter regions of TET genes, leading to decreased expression levels. Treatment with 5-AZA or EGCG significantly reduced bacterial reproduction and inhibited methionine synthase expression.
JOURNAL OF MEDICAL MICROBIOLOGY
(2023)
Article
Virology
Mai Alalem, Emad Dabous, Ahmed M. Awad, Nedaa Alalem, Adel A. Guirgis, Samir El-Masry, Hany Khalil
Summary: Research has revealed the significant role of miR-141 in influenza virus infection, where it regulates virus replication by modulating the interferon signaling pathway. The downregulation of several genes, including MxA, STAT3, IFI27, and LAMP3, by miR-141 affects the expression of antiviral genes induced by interferon and IL-6 signaling. This study is of great importance in understanding the immune response mechanisms during influenza virus infection.
Article
Oncology
Amira Abd El-Hady, Farha A. El-Chennawi, Bahgat El-Fikiy, Ibrahim H. El-Sayed, Hany Khalil
Summary: This experimental study investigated the anticancer properties of interferon-gamma, rapamycin, and vitamin B17 in hepatocellular carcinoma and stem cells. Vitamin B17 showed competitive inhibition on cancer cell proliferation compared to Sorafenib, while exerting different effects on stem cells. Vitamin B17 selectively regulated the expression of Raf-1 and autophagy-related LC3B in cancer cells, indicating its effectiveness in cancer treatment.
MIDDLE EAST JOURNAL OF CANCER
(2023)
Letter
Respiratory System
Theresa A. Laguna, Benjamin T. Kopp, D'Ann Brown-Janowiak, Nancy Guerrero, Liliana Rose, Brandie Wagner, Paul E. Moore
ANNALS OF THE AMERICAN THORACIC SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Tarek Fekry, Mohamed F. Salem, Amal A. Abd-Elaziz, Shaden Muawia, Yahya M. Naguib, Hany Khalil
Summary: This study demonstrated the anticancer activity of Se-enriched mushrooms in colon cancer cells by targeting the Raf-1 signaling pathway and increasing the production of IL-6 and IL-10. The mushrooms were able to accumulate and convert inorganic selenium to the organic form during growth.
INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS
(2022)