Nuclear pores as versatile reference standards for quantitative superresolution microscopy

Quantifying Protein Copy Number in Super-Resolution Using an Imaging-Invariant Calibration

(2019) Francesca Cella Zanacchi et al.   BIOPHYSICAL JOURNAL

Measuring expansion from macro‐ to nanoscale using NPC as intrinsic reporter

(2019) Luca Pesce et al.   Journal of Biophotonics

Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software

(2019) Daniel Sage et al.   NATURE METHODS

Direct visualization of single nuclear pore complex proteins using genetically-encoded probes for DNA-PAINT

(2019) Thomas Schlichthaerle et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Synthetic and genetic dimers as quantification ruler for single-molecule counting with PALM

(2019) Tim N. Baldering et al.   MOLECULAR BIOLOGY OF THE CELL

Real-time 3D single-molecule localization using experimental point spread functions

(2018) Yiming Li et al.   NATURE METHODS

Quantitative mapping and minimization of super-resolution optical imaging artifacts

(2018) Siân Culley et al.   NATURE METHODS

Generation and validation of homozygous fluorescent knock-in cells using CRISPR–Cas9 genome editing

(2018) Birgit Koch et al.   Nature Protocols

Stoichiometry and compositional plasticity of the yeast nuclear pore complex revealed by quantitative fluorescence microscopy

(2018) Sasikumar Rajoo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Systematic Nanoscale Analysis of Endocytosis Links Efficient Vesicle Formation to Patterned Actin Nucleation

(2018) Markus Mund et al.   CELL

Integrative structure and functional anatomy of a nuclear pore complex

(2018) Seung Joong Kim et al.   NATURE

Imaging cellular ultrastructures using expansion microscopy (U-ExM)

(2018) Davide Gambarotto et al.   NATURE METHODS

A DNA origami platform for quantifying protein copy number in super-resolution

(2017) Francesca Cella Zanacchi et al.   NATURE METHODS

Strong signal increase in STED fluorescence microscopy by imaging regions of subdiffraction extent

(2017) Fabian Göttfert et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies

(2016) Paul W Tillberg et al.   NATURE BIOTECHNOLOGY

Bright photoactivatable fluorophores for single-molecule imaging

(2016) Jonathan B Grimm et al.   NATURE METHODS

Efficient homogeneous illumination and optical sectioning for quantitative single-molecule localization microscopy

(2016) Joran Deschamps et al.   OPTICS EXPRESS

Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations

(2016) Nils Gustafsson et al.   Nature Communications

Nuclear pore assembly proceeds by an inside-out extrusion of the nuclear envelope

(2016) Shotaro Otsuka et al.   eLife

A Set of Homo-Oligomeric Standards Allows Accurate Protein Counting

(2015) Kieran Finan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Heavy water: a simple solution to increasing the brightness of fluorescent proteins in super-resolution imaging

(2015) Wei Qiang Ong et al.   CHEMICAL COMMUNICATIONS

Assessing resolution in super-resolution imaging

(2015) Justin Demmerle et al.   METHODS

In situ structural analysis of the human nuclear pore complex

(2015) Alexander von Appen et al.   NATURE

Expansion microscopy

(2015) F. Chen et al.   SCIENCE

One, two or three? Probing the stoichiometry of membrane proteins by single-molecule localization microscopy

(2015) Franziska Fricke et al.   Scientific Reports

SIMcheck: a Toolbox for Successful Super-resolution Structured Illumination Microscopy

(2015) Graeme Ball et al.   Scientific Reports

Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation

(2015) Tino Pleiner et al.   eLife

Single-molecule evaluation of fluorescent protein photoactivation efficiency using an in vivo nanotemplate

(2014) Nela Durisic et al.   NATURE METHODS

3D superresolution microscopy by supercritical angle detection

(2014) Joran Deschamps et al.   OPTICS EXPRESS

Characterization and development of photoactivatable fluorescent proteins for single-molecule-based superresolution imaging

(2014) S. Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Stochastic approach to the molecular counting problem in superresolution microscopy

(2014) Geoffrey C. Rollins et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Polyhedra Self-Assembled from DNA Tripods and Characterized with 3D DNA-PAINT

(2014) R. Iinuma et al.   SCIENCE

Increasing the Brightness of Cyanine Fluorophores for Single-Molecule and Superresolution Imaging

(2013) Kathrin Klehs et al.   CHEMPHYSCHEM

Cell type-specific nuclear pores: a case in point for context-dependent stoichiometry of molecular machines

(2013) A. Ori et al.   Molecular Systems Biology

Measuring image resolution in optical nanoscopy

(2013) Robert P J Nieuwenhuizen et al.   NATURE METHODS

Counting molecules in single organelles with superresolution microscopy allows tracking of the endosome maturation trajectory

(2013) E. M. Puchner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nuclear Pore Scaffold Structure Analyzed by Super-Resolution Microscopy and Particle Averaging

(2013) A. Szymborska et al.   SCIENCE

Super-resolution imaging visualizes the eightfold symmetry of gp210 proteins around the nuclear pore complex and resolves the central channel with nanometer resolution

(2012) A. Loschberger et al.   JOURNAL OF CELL SCIENCE

A simple, versatile method for GFP-based super-resolution microscopy via nanobodies

(2012) Jonas Ries et al.   NATURE METHODS

mMaple: A Photoconvertible Fluorescent Protein for Use in Multiple Imaging Modalities

(2012) Ann L. McEvoy et al.   PLoS One

Counting single photoactivatable fluorescent molecules by photoactivated localization microscopy (PALM)

(2012) S.-H. Lee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Actin, Spectrin, and Associated Proteins Form a Periodic Cytoskeletal Structure in Axons

(2012) K. Xu et al.   SCIENCE

Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging

(2011) Xiaoli Sun et al.   CHEMBIOCHEM

Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging

(2011) Graham T Dempsey et al.   NATURE METHODS

Quantitative Photo Activated Localization Microscopy: Unraveling the Effects of Photoblinking

(2011) Paolo Annibale et al.   PLoS One

Seamless Gene Tagging by Endonuclease-Driven Homologous Recombination

(2011) Anton Khmelinskii et al.   PLoS One

Fast, single-molecule localization that achieves theoretically minimum uncertainty

(2010) Carlas S Smith et al.   NATURE METHODS

Optimized localization analysis for single-molecule tracking and super-resolution microscopy

(2010) Kim I Mortensen et al.   NATURE METHODS

DNA Origami as a Nanoscopic Ruler for Super-Resolution Microscopy

(2009) Christian Steinhauer et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis

(2008) Georgyi V. Los et al.   ACS Chemical Biology

Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution

(2008) Bo Huang et al.   NATURE METHODS

Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy

(2008) B. Huang et al.   SCIENCE