Microstructure evolution of sandwich graphite oxide/interlayer-embedded Au nanoparticles induced from γ-rays for carcinoembryonic antigen biosensor

With the capability of inducing small particle sizes of supported metal in graphite oxide (GO), the gamma-ray irradiation method applied for preparing graphite oxide-gold (GO-Au) nanocomposites as electrochemical immunosensors has attracted specific attention recently. To study the accurate factors influencing the precise morphology and final performance of the prepared composites in the gamma-irradiation system, we proposed a facile method to investigate the evolution of the GO structure, size and dispersion of Au nanoparticles (AuNPs) produced with the addition of isopropyl alcohol to the system. The GO-Au nanocomposites were characterized by Fourier transform infrared spectroscopy, x-ray diffraction spectra, Raman spectra, x-ray photoelectron spectroscopy and high resolution transmission electron microscopy. These nanocomposites with sandwich morphology exhibited an excellent immunosensor performance with a low detection limit of 15.8 pg ml(-1) (S/N = 3) and a wide linear range from 1 to 40 ng ml(-1) for detecting carcinoembryonic antigens. The enhanced biosensing performance is attributed to the synergistic effect of gamma-irradiation and the precise structure of GO, which endows the smaller size and more uniform distribution of AuNPs on the GO as well as the good signal amplification capability. Furthermore, adopting the gamma-irradiation method and use of GO as a precursor is propitious for application in large-scale production because of its high-efficiency and high-yielding characteristics.