期刊
JOURNAL OF PLANT DISEASES AND PROTECTION
卷 126, 期 6, 页码 517-528出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s41348-019-00258-0
关键词
Pseudomonas amygdali pv; lachrymans; Detection; nfrB gene; Leaf rinse; Real-time PCR
资金
- Project EFRR Multidisciplinary research to increase application potential of nanomaterials in agricultural practice [CZ.02.1.01/0.0/0.0/16_025/0007314]
- [LTC18009]
- [TJ01000274]
Pseudomonas amygdali pv. lachrymans (Pal) is a bacterium that causes angular leaf spot disease of cucumbers. There is no direct protection against this pathogen, only prevention. Previously published detection systems are labour-intensive and time-consuming. The aim of this study was to create a rapid and reliable diagnostic assay for the detection of Pal in cucumber plants without the need to destroy or damage the plant. The created method is based on washing the bacteria off the leaf surface with saline solution and using the sample directly in a quantitative real-time PCR assay. This detection system is based on the specific amplification of a small coding region of the bacteriophage N4 adsorption protein B gene. The assay was tested on 120 plant samples inoculated with seven different Pal strains. The detection limit of the assay was 2.60 x 10(2) CFU ml(-1), and the concentration of Pal in the leaf rinse ranged from 2.71 x 10(2) to 6.94 x 10(7) CFU ml(-1) depending on the particular strain. This assay is a reliable and rapid diagnostic method for the detection of Pal from cucumber leaves and is valuable for contributing to angular leaf spot management.
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