4.7 Article

High-Throughput PET/CT Imaging Using a Multiple-Mouse Imaging System

期刊

JOURNAL OF NUCLEAR MEDICINE
卷 61, 期 2, 页码 292-297

出版社

SOC NUCLEAR MEDICINE INC
DOI: 10.2967/jnumed.119.228692

关键词

high-throughput; mouse hotel; micro PET/CT; F-18-FDG; economic

资金

  1. Wellcome Trust
  2. Royal Society Sir Henry Dale Fellowship [107610/Z/15/Z]
  3. CRUK UCL Centre Non-Clinical Training Award [A23233]
  4. Wellcome Trust [107610/Z/15/Z] Funding Source: Wellcome Trust

向作者/读者索取更多资源

A considerable limitation of current small-animal PET/CT imaging is the low throughput of acquisitions. Consequently, to sufficiently power a study, high costs accumulate. Together with a commercial scanner manufacturer, we developed a 4-bed mouse hotel to simultaneously image up to 4 mice, thereby reducing costs and maximizing the efficiency of radiotracer use when compared with scans performed with a single mouse bed. Methods: For physiologic evaluation of the mouse hotel, temperature and anesthesia were tested for uniformity in conjunction with F-18-FDG PET/CT imaging of mini image-quality phantoms designed to fit the new imaging system. After reconstruction, National Electrical Manufacturers Association NU-4 tests examined uniformity, recovery coefficients, and spillover ratios. To evaluate the mouse hotel under standard in vivo imaging conditions, 4 mice were simultaneously scanned by dynamic F-18-FDG PET/CT over 60 min, and quantified images were compared with those acquired using a single mouse bed. Results: The mouse hotel maintained a constant temperature of 36.8 degrees C +/- 0.4 degrees C, with anesthesia distributed evenly to each nose cone (2.9 +/- 0.1 L/min). The National Electrical Manufacturers Association tests revealed values within tolerable limits for uniformity, for recovery coefficients in rods larger than 2 mm, and for spillover ratios in the nonradioactive water- and air-filled chambers. There was low variability in radiotracer uptake in all major organs for the mouse hotel versus the single mouse bed. Conclusion: Analysis of images acquired using the mouse hotel confirmed its utility to increase the throughput of small-animal PET imaging without considerable loss of image quality or quantitative precision. In comparison to a single mouse bed, the cost and time associated with each scan were substantially reduced.

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