期刊
JOURNAL OF FISH DISEASES
卷 42, 期 10, 页码 1391-1399出版社
WILEY
DOI: 10.1111/jfd.13059
关键词
cell line; cytopathic effect; immune response; Pampus argenteus; viral haemorrhagic septicaemia virus
资金
- National Natural Science Foundation of China [31602191, 31771587]
- Natural Science Foundation of Guangdong Province [2015A030308012]
- Zhuhai Scholar Professor Program [2015]
- Pearl River S & T Nova Program of Guangzhou [201806010047]
- Fundamental Research Funds for the Central Universities [17lgpy61]
A cell line (PaF) derived from the fin tissue of silver pomfret (Pampus argenteus) was established and characterized in this study. The cell line has been subcultured for more than 50 times in Dulbecco's modified Eagle's medium (DMEM) containing 15% foetal bovine serum (FBS) since the initial primary culture. PaF cells grew well at temperatures from 24 degrees C to 28 degrees C in DMEM supplemented with 15% FBS. Partial amplification and sequence analysis of the cytochrome B gene indicated that PaF originated from silver pomfret. Cytogenetic analysis demonstrated that the modal chromosome number was 48. A significant cytopathic effect was observed in PaF cells during viral haemorrhagic septicaemia virus (VHSV) infection, and the VHSV replication was confirmed by qRT-PCR and viral titre assays. In contrast, PaF cells were resistant to red-spotted grouper nervous necrosis virus infection. Moreover, PaF cells could respond to VHSV and lipopolysaccharide treatments, as indicated by the expression of immune-related genes, TLR5 and TLR9. In conclusion, the establishment of PaF cell line will provide an appropriate in vitro tool for the study of mechanisms of pathogen-silver pomfret interaction.
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