Article
Environmental Sciences
Lorena Selak, Tamara Markovic, Petra Pjevac, Sandi Orlic
Summary: Climate change-induced rising sea levels and prolonged dry periods pose a global threat to freshwater scarcity, particularly through salinization. This study focused on the impact of anthropogenic activities and drought-induced salinization on microbial communities in Lake Vrana and its inlets. Microbial community spatial differences were driven by salinity, drought, and nutrient loading, with certain halotolerant bacteria taxa emerging as potential indicators for salinization. Despite increased precipitation, the lake displayed increased salinity, and lake water column homogenization took time after summer salinization. This study contributes to our understanding of the Freshwater Salinization Syndrome's impact on Mediterranean lakes' microbial communities and ecosystem resilience.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Agriculture, Dairy & Animal Science
Fabio V. R. Portilho, Juliano Nobrega, Beatriz O. de Almeida, Andre R. Mota, Carolina L. de Paula, Fernando J. P. Listoni, Sandra M. G. Bosco, Alana L. Oliveira, Maria de Lourdes R. S. Cunha, Marcio G. Ribeiro
Summary: We investigated the oral microbiota of 100 healthy dogs and identified a complex community of bacteria and fungi, including potential pathogens to humans. We also observed multiple drug-resistant bacteria in the oral cavity of these dogs. This highlights the risk of transmission of microorganisms from dogs to humans and the challenges in treating infections caused by dog bites.
Article
Environmental Sciences
Josenilda Carlos dos Santos, Deborah Romaskevis Gomes Lopes, Livia Carneiro Fidelis Silva, Jose Luiz Lima Ramos, Roberto Sousa Dias, Helena Santiago Lima, Maira Paula de Sousa, Vinicius de Abreu Waldow, Sergio Oliveira de Paula, Sukarno Olavo Ferreira, Cynthia Canedo da Silva
Summary: The study evaluated the impact of different carbon sources on biofilm structure and bacterial diversity, showcasing that the biofilms formed densely without morphology and microbial density differences, while the planktonic bacterial community exhibited higher species diversity indices compared to the biofilm.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Food Science & Technology
Yuzhao Ling, Hongyuan Tan, Lingwei Shen, Lingyun Wei, Guangquan Xiong, Lan Wang, Wenjin Wu, Yu Qiao
Summary: The effects of ozone water and ultrasound cleaning on the microbial community diversity of crayfish were studied through microbial viable count and 16S rRNA gene sequencing. The results showed that the combined treatment significantly reduced the viable count of various microorganisms. Sensory evaluation indicated that the crayfish treated with ozone water and ultrasound cleaning provided a satisfactory sensory experience.
Article
Immunology
Miquel Rozas, Francois Brillet, Chris Callewaert, Bernhard Paetzold
Summary: This study used MinION(TM) nanopore sequencing technology to classify and analyze skin bacteria, finding that the existing protocols have biases in estimating the relative abundance of certain skin microbiome genera. By changing the amplification protocols, the accuracy of taxonomic classification for these three main skin bacterial genera can be improved. The study shows that MinION(TM) nanopore technology has great potential in studying skin microbiomes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Changwoo Park, Seung Bum Kim, Sang Ho Choi, Seil Kim
Summary: Microbial community analysis based on the 16S rRNA-gene is important for studying microorganisms, and next-generation sequencing technology has improved the accuracy and speed of analysis. However, biases can still occur in the results due to factors like sample handling and sequencing platforms. Different primer pairs and sequencing platforms can lead to varying levels of bias in microbial community analysis.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Engineering, Environmental
Nathan L. R. Williams, Nachshon Siboni, Jaimie Potts, Meredith Campey, Colin Johnson, Shivanesh Rao, Anna Bramucci, Peter Scanes, Justin R. Seymour
Summary: By using molecular microbiological approaches, the causes and point sources of faecal contamination at an urbanised beach have been precisely pinpointed, which helps identify the most suitable locations for targeted management of water quality at the beach.
Article
Microbiology
Jinru Yang, Yuxi Ma, Qiaoyun Tan, Bin Zhou, Dandan Yu, Min Jin, Tao Zhang, Junli Liu, Hongli Liu
Summary: This study found significant differences in gut microbiome composition between pancreatic cancer patients and healthy individuals, and identified Streptococcus as a potential biomarker for early prediction of pancreatic cancer and liver metastasis. This is critical for early diagnosis of diseases.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Panpan Zhang, Xuehua Zhou, Hong Tan, Fangfang Jian, Zenghui Jing, Huajie Wu, Yao Zhang, Jianfeng Luo, Juan Zhang, Xin Sun
Summary: This study investigated the changes in gut microbiota of allergic rhinitis (AR) children in Chinese Mainland and evaluated the correlation between gut microbiota and clinical indexes. The results showed that AR children had lower microbial diversity and distinct microbial characteristics compared to healthy control children. These findings indicate the presence of unique gut microbiota profiles in AR children and highlight the need for further mechanistic studies.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agronomy
Andjin Siegenthaler, Andrew K. Skidmore, G. Arjen de Groot, Ivo Laros, Melody Rousseau, Yiwei Duan
Summary: This study reveals sphere-specific microbial communities in European temperate forests, characterized by low connectivity. It emphasizes the stronger influence of microbial spheres rather than host identity. Only a few taxa inhabit both the top-canopy and soil spheres, yet they make up the majority of the abundance in each sphere.
Article
Environmental Sciences
Alice Delacuvellerie, Axelle Brusselman, Valentine Cyriaque, Samira Benali, Sebastien Moins, Jean-Marie Raquez, Sylvie Gobert, Ruddy Wattiez
Summary: The best-selling compostable plastics, PLA and PBAT, can enter the marine environment accidentally due to mismanagement of plastic waste. However, their degradation and colonization by microbial communities in marine conditions are not well-documented. In this study, PBAT, semicrystalline, and amorphous PLA films were immersed in a marine aquarium for 99, 160, and 260 days to investigate their degradation and bacterial colonization. The results showed that degradation of these plastics was slow and the microbial community structure varied with immersion time, with a high proportion of Archaea. Understanding the degradability of compostable plastics is crucial for assessing their impact on ecosystems and designing new recyclable plastics with optimal degradation properties.
MARINE POLLUTION BULLETIN
(2023)
Article
Zoology
Liping Tang, Liping Yan, Huiping Jia, Yu Xiong, Xinping Ma, Hongjun Chu, Zhicheng Sun, Liang Wang, Mubalake Shalitanati, Kai Li, Defu Hu, Dong Zhang
Summary: Host-associated microbiota can have a significant impact on host fitness, and variations in microbiota may affect the health and survival of hosts. Little is known about how microbiomes are altered in response to conservation translocation, especially in reintroduced animals.
INTEGRATIVE ZOOLOGY
(2023)
Article
Microbiology
Alba Regueira-Iglesias, Lara Vazquez-Gonzalez, Carlos Balsa-Castro, Nicolas Vila-Blanco, Triana Blanco-Pintos, Javier Tamames, Maria Jose Carreira, Inmaculada Tomas
Summary: This study analyzed the coverage of 16S rRNA gene primers used in oral microbiome studies and identified the best primers. The findings showed that the optimal primers can be utilized for detecting both oral bacteria and archaea, with a wide coverage range.
Article
Microbiology
Wilbert Gutierrez-Sarmiento, Betsy Anaid Pena-Ocana, Anayancy Lam-Gutierrez, Jorge Martin Guzman-Albores, Ricardo Jasso-Chavez, Victor Manuel Ruiz-Valdiviezo
Summary: This study analyzed the physicochemical characteristics, microbial communities, and predictive metabolic functionalities of Tepache, a native beverage from Mexico. The research revealed significant changes in the physicochemical properties and microbial communities during the fermentation process. These findings contribute to the understanding of Tepache manufacturing and its potential health benefits.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Microbiology
Virginie Lemieux-Labonte, Chloe Vigliotti, Zoran Tadic, Beck Wehrle, Philippe Lopez, Eric Bapteste, Francois-Joseph Lapointe, Donovan P. German, Anthony Herrel
Summary: Dietary changes are important factors driving variation in mammalian gut microbiota composition, but other factors such as insularity and population of origin also contribute to differences in microbial composition.
Article
Multidisciplinary Sciences
Sonia Szymanska, Marcin Sikora, Katarzyna Hrynkiewicz, Jaroslaw Tyburski, Andrzej Tretyn, Marcin Golebiewski
Summary: The study assessed the diversity of endophytic bacteria in sugar and sea beet roots, and the influence of osmoprotectants during lyophilization on bacterial density, viability, and salt tolerance. Sea beet's endophytic microbiome is more diverse and salt resistant than its crop relative, and supplementing trehalose during lyophilization is more effective in enhancing bacterial community salt stress tolerance, viability, and density. This suggests the potential use of trehalose in formulating improved biofertilizers.
SCIENTIFIC REPORTS
(2021)
Article
Nutrition & Dietetics
Ewa Los-Rycharska, Marcin Golebiewski, Marcin Sikora, Tomasz Grzybowski, Marta Gorzkiewicz, Maria Popielarz, Julia Gawryjolek, Aneta Krogulska
Summary: This study found a relationship between gut and skin microbiota in patients with allergies, especially in terms of bacterial community structure and allergy phenotypes. Different clinical conditions were associated with characteristic bacterial taxa in the skin and gut microbiota, and while infants with allergies and healthy infants showed similar alpha-diversity in their microbiota, differences in beta-diversity and bacterial species abundance were observed. Positive correlations were found between certain bacterial taxa in skin and fecal samples, which may serve as indicators of the host's allergic state.
Article
Biochemistry & Molecular Biology
Karolina Majewska, Patrycja Wroblewska-Ankiewicz, Magda Rudzka, Malwina Hyjek-Skladanowska, Marcin Golebiewski, Dariusz Jan Smolinski, Agnieszka Kolowerzo-Lubnau
Summary: Recent studies have highlighted the crucial role of post-transcriptional processes in gene expression regulation, particularly the retention of mRNA in the nucleus. Analysis of larch microsporocytes revealed two main patterns of nuclear accumulation and transport of transcripts, indicating an evolutionarily conserved mechanism of gene regulation across various cell types.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Piotr Koczorski, Bliss Ursula Furtado, Marcin Golebiewski, Piotr Hulisz, Christel Baum, Martin Weih, Katarzyna Hrynkiewicz
Summary: The study revealed that the low availability of phosphorus in soil is mainly caused by the high iron content in the clay fraction that binds to phosphorus, making it unavailable. The level of plant association, plant genotype, and environmental conditions all have significant effects on the abundance and diversity of phosphorus solubilizing microorganisms in soil.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Cell Biology
Andrzej Tretyn, Joanna Szczepanek, Monika Skorupa, Joanna Jarkiewicz-Tretyn, Dorota Sandomierz, Joanna Dejewska, Karolina Ciechanowska, Aleksander Jarkiewicz-Tretyn, Wojciech Koper, Krzysztof Palgan
Summary: The study evaluated the antibody levels against SARS-CoV-2 virus and cellular immune response after the vaccination with mRNA vaccines. High levels of antibodies were observed in 98% of the vaccinated cohort, and all participants had protective T cells. The immune response is diversified, visible as early as 2-3 weeks after the first dose, and the level of protection significantly increased after the second dose, especially in pre-vaccine healthy subjects.
Article
Nutrition & Dietetics
Marcin Golebiewski, Ewa Los-Rycharska, Marcin Sikora, Tomasz Grzybowski, Marta Gorzkiewicz, Aneta Krogulska
Summary: The study indicates that mother's milk influences the fecal and skin microbiomes of infants, with specific taxa potentially playing a vital role in maintaining health or allergy development.
Article
Immunology
Joanna Szczepanek, Monika Skorupa, Agnieszka Goroncy, Joanna Jarkiewicz-Tretyn, Aleksandra Wypych, Dorota Sandomierz, Aleksander Jarkiewicz-Tretyn, Joanna Dejewska, Karolina Ciechanowska, Krzysztof Palgan, Pawel Rajewski, Andrzej Tretyn
Summary: The study found that mRNA vaccines induce a stronger humoral response of the immune system, with the fastest decline in antibody levels over time.
Article
Biochemistry & Molecular Biology
Magda Rudzka, Patrycja Wroblewska-Ankiewicz, Karolina Majewska, Malwina Hyjek-Skladanowska, Marcin Golebiewski, Marcin Sikora, Dariusz Jan Smolinski, Agnieszka Kolowerzo-Lubnau
Summary: Our study reveals a novel mechanism of gene expression regulation during generative cell development in plants, based on pre-mRNA retention in the nucleus. This phenomenon has a significant impact on gene expression by regulating export and translation delay, allowing synthesis of specific proteins at specific time points.
Review
Cell Biology
Joanna Szczepanek, Monika Skorupa, Andrzej Tretyn
Summary: miRNAs are not only markers of neoplastic disease initiation and progression, but also markers of response to anticancer therapy. Several miRNAs have been identified as biomarkers of drug resistance and have potential to sensitize cancer cells to therapy. The use of miRNAs in cancer treatment has been verified in clinical trials, showing great potential.
Article
Immunology
Monika Skorupa, Joanna Szczepanek, Agnieszka Goroncy, Joanna Jarkiewicz-Tretyn, Barbara Ptaszynska, Pawel Rajewski, Wojciech Koper, Krzysztof Palgan, Andrzej Tretyn
Summary: The characteristics of changes in anti-SARS-CoV-2 IgG within one year of vaccination in healthcare workers were investigated. It was found that booster doses significantly increased the levels of IgG antibodies and could have a real impact on restricting the further transmission of the virus.
Article
Microbiology
Piotr Koczorski, Bliss Ursula Furtado, Marcin Golebiewski, Piotr Hulisz, Dominika Thiem, Christel Baum, Martin Weih, Katarzyna Hrynkiewicz
Summary: Phosphorus is an essential plant nutrient that can limit plant growth due to low availability in the soil. This study investigated the impact of plant species, plant association with bacteria, and environmental factors on the abundance and diversity of phosphorus-solubilizing bacteria in willow short-rotation coppices. The results showed that soil properties and plant-bacteria association were the main factors shaping the bacterial communities.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Palgan Krzysztof, Tretyn Andrzej
Summary: Anaphylaxis is a life-threatening hypersensitivity reaction, often caused by drugs and food. Factors such as physical exercise, acute infections, drugs, alcohol, and menstruation can exacerbate the severity of the reaction. This review highlights the role of platelet-activating factor in the development of severe anaphylactic reactions, including anaphylactic shock.
Review
Biochemistry & Molecular Biology
Joanna Szczepanek, Monika Skorupa, Joanna Jarkiewicz-Tretyn, Cezary Cybulski, Andrzej Tretyn
Summary: Breast cancer demonstrates diverse epigenetic abnormalities that impact gene expression and tumor characteristics. Epigenetic alterations are crucial in cancer development and progression, and drugs targeting epigenetic modifications show promise for cancer treatment, including DNA methyltransferase inhibitors, histone-modifying enzymes, and mRNA regulators. However, currently, there is no effective standalone epigenetic drug therapy for breast cancer. Combining epigenetic drugs with conventional therapies has shown positive results, and targeting specific epigenetic changes may lead to more effective monotherapy options in the future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Ecology
Beata Klimek, Malgorzata Jazwa, Marcin Golebiewski, Marcin Sikora, Edyta Deja-Sikora
ACTA OECOLOGICA-INTERNATIONAL JOURNAL OF ECOLOGY
(2020)