期刊
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
卷 74, 期 -, 页码 32-38出版社
OXFORD UNIV PRESS
DOI: 10.1093/jac/dkz285
关键词
-
资金
- University of Manitoba
- Diagnostic Services Manitoba
- National Microbiology Laboratory
- Astellas
- Merck
- Pfizer
- Sunovion
- The Medicines Company
- Abbott
- Achaogen
- Cubist
- Paladin Labs
- Bayer
- Janssen Ortho/Ortho McNeil
- Affinium
- Basilea
- AstraZeneca
- Paratek
- Tetraphase
- Theravance
- Sanofi-Aventis
- Zoetis
Objectives: Carbapenem-resistant Pseudomonas aeruginosa are emerging worldwide with increasing reports of carbapenemase-producing isolates. Carbapenem-resistant isolates may also be XDR. This study characterized carbapenem-resistant and XDR P. aeruginosa isolated from patients receiving care at Canadian hospitals from 2007 to 2016. Methods: Antimicrobial susceptibility testing was performed using CLSI broth microdilution methods. PCR was used to detect carbapenemases (GES, KPC, NDM, IMP, VIM, OXA-48) and other resistance markers; specific carbapenemase gene variants were identified by DNA sequencing. Genetic relatedness was assessed by MLST and PFGE. Results: From 2007 to 2016, 3864 isolates of P. aeruginosa were collected; 466 (12.1%) isolates were carbapenem resistant. The prevalence of carbapenem-resistant P. aeruginosa reached a peak of 17.3% in 2014. Colistin (94% susceptible) and ceftolozane/tazobactam (92.5%) were the most active agents against carbapenem-resistant P. aeruginosa. XDR P. aeruginosa comprised 4.5% of isolates; they were found to be genetically diverse and remained susceptible to colistin and ceftolozane/tazobactam. Only 4.3% (n=20) of carbapenem-resistant P. aeruginosa harboured a carbapenemase; most were bla(GES-5) (35%, n=7). Wide genetic diversity was observed among carbapenem-resistant P. aeruginosa with >200 different sequence types identified. Conclusions: Although the prevalence of carbapenem-resistant P. aeruginosa in Canada spiked in 2014 and 2015, carbapenemase-producing P. aeruginosa remain rare with only 20 (4.3%) isolates identified over a 10 year period. Broad genetic diversity was observed among both carbapenem-resistant and XDR phenotypes of P. aeruginosa. Pan-drug-resistant P. aeruginosa have not yet been identified in Canada.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据