Article
Food Science & Technology
Wei Fan, Xiao-yue Gao, He-nan Li, Wen-ping Guo, Ying-ying Li, Shou-wei Wang
Summary: This study aimed to develop a method combining IMS and qPCR techniques for rapid and accurate detection of three pathogenic bacteria in meat samples. The method showed high capture efficiency, good repeatability, and low detection limits.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Tao Yang, Zhenghui Wang, Yang Song, Xinyan Yang, Sihan Chen, Shiqian Fu, Xue Qin, Wei Zhang, Chaoxin Man, Yujun Jiang
Summary: A smartphone-based colorimetric aptasensor was developed for the detection of E. coli O157:H7 in milk, showing good reproducibility with no cross-reaction for other bacteria. The sensor could detect a concentration of 8.43 x 10^3 cfu/mL of E. coli O157:H7 in pure culture and 5.24 x 10^2 cfu/mL in artificially contaminated milk after 1 hour of incubation.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Chemistry, Analytical
Zhihua Li, Xue Zhang, Haishan Qi, Xiaowei Huang, Jiyong Shi, Xiaobo Zou
Summary: A renewable electrochemical biosensor based on mussel-inspired adhesive protein was developed for the detection of Escherichia coli O157:H7. The biosensor showed excellent repeatability and sensitivity, with a wide linear range and a low detection limit. It demonstrated good performance in detecting E. coli O157:H7 in seafood samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Sarah H. Klass, Laura E. Sofen, Zachary F. Hallberg, Tahoe A. Fiala, Alexandra Ramsey, Nicholas S. Dolan, Matthew B. Francis, Ariel L. Furst
Summary: Pathogenic E. coli strains are a significant threat to public health, causing both foodborne illnesses and urinary tract infections. By using a rapid bioconjugation reaction and electrochemical impedance spectroscopy, we can selectively capture and accurately quantify these pathogenic microbes on a disposable gold electrode.
CHEMICAL COMMUNICATIONS
(2021)
Article
Veterinary Sciences
Delphine Bibbal, Philippe Ruiz, Panagiotis Sapountzis, Christine Mazuy-Cruchaudet, Estelle Loukiadis, Frederic Auvray, Evelyne Forano, Hubert Brugere
Summary: This study aimed to identify farms with persistent circulation of EHEC O157:H7 in cattle. The results showed that persistent circulation may be due to the presence of a few predominant strains or repeated exposure of cattle to various strains. In addition, differences were found in the fecal microbial communities of shedders and non-shedders, with different bacterial indicators identified.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Food Science & Technology
Jeong-Eun Hyun, Sung-Kwon Moon, Sun-Young Lee
Summary: The study showed that the combination of blue LED with chemicals such as carvacrol can effectively reduce populations of E. coli O157:H7 on fresh produce, indicating a potential application for extending shelf-life without causing color deterioration.
Article
Food Science & Technology
Kai Dong, Yuwan Li, Sijia Luo, Fan Zhang, Hanxu Pan, Liang Zhao, Yongtao Wang, Xiaojun Liao
Summary: The study analyzed the sterilizing effects of different HHP-boosting processes on Escherichia coli O157:H7, finding that linear and fast boosting had the most significant bactericidal effects. The proteins interacting with GroEL under different boost modes and rates were involved in 42 metabolic pathways, particularly ribosome, ABC transporter, oxidative phosphorylation, sulfate assimilation, and cysteine biosynthesis. Key interacting proteins NuoB, NuoF, CysD, ZnuC, and RplX were identified and their interactions with GroEL were verified in vivo.
Article
Immunology
Juliana Gonzalez, Jimena Soledad Cadona, Claudio Marcelo Zotta, Silvina Lavayen, Roberto Vidal, Nora Lia Padola, Andrea Mariel Sanso, Ana Victoria Bustamante
Summary: This study aimed to compare the genetic diversity of VTEC O157:H7 strains isolated from human disease cases in Argentina and Chile. The results showed that these strains mainly belonged to lineage I/II, associated with hypervirulent strains, and were categorized into the phylogroup E. Additionally, genetic diversity was observed among Argentinean and Chilean strains, particularly in relation to putative virulence determinants and nle profiles.
MICROBES AND INFECTION
(2022)
Article
Veterinary Sciences
Aklilu Feleke Haile, Silvia Alonso, Nega Berhe, Tizeta Bekele Atoma, Prosper N. Boyaka, Delia Grace
Summary: This study estimated the prevalence and antimicrobial resistance of Escherichia coli O157:H7 in raw beef sold in butcher shops in Addis Ababa, Ethiopia. Out of the 384 samples examined, 3.64% carried E. coli O157:H7, with 57.14% of the isolates showing resistance to three or more antimicrobial agents. Thus, stricter monitoring of antimicrobial use and further studies on contamination sources and risk mitigation strategies are needed.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Chemistry, Analytical
Manman Du, Jinbiao Ma, Zhiwei Zhang, Guangzu Wu, Jianguo Wu, Hao Wang, Xinwu Xie, Can Wang
Summary: This study developed a rGO-FET biosensor for the rapid and sensitive detection of pathogenic microorganisms. The biosensor demonstrated good specificity, a wide linear range, and the ability to directly detect real samples without pretreatment.
ANALYTICA CHIMICA ACTA
(2023)
Article
Microbiology
Aklilu Feleke Haile, Silvia Alonso, Nega Berhe, Tizeta Bekele Atoma, Prosper N. Boyaka, Delia Grace
Summary: This study examined the prevalence and antimicrobial resistance of E. coli O157:H7 in lettuce in Addis Ababa, Ethiopia. The results indicated a low prevalence but presence of drug-resistant strains, highlighting a potential public health risk associated with this pathogen in the area.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Jiye Li, Jie Wang
Summary: This study demonstrates the use of extinction spectrum to quickly transform the signal of MoS2-NS aggregation and reveals the size-dependent extinction response. Additionally, the size-dependent optical extinction behavior of MoS2-NSs with aptamer-induced dispersion is studied to improve target identification ability. This approach has been successfully employed for label-free detection of Escherichia coli O157:H7.
Article
Acoustics
Jiao Li, Donghong Liu, Tian Ding
Summary: The study investigated the gene regulation at the transcription level in Escherichia coli O157:H7 after ultrasonic stimulation, revealing significant differential expressions of 1217 genes, including up-regulated and down-regulated genes related to crucial biomolecular processes. The most enriched pathways were analyzed in carbohydrate metabolism, energy metabolism, membrane transport, signal transduction, transcription, and translation categories. Additionally, the study found that the citrate cycle genes were down-regulated, affecting carbohydrate decomposition and energy production under ultrasonic stress.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Food Science & Technology
Dianbo Zhao, Shaodan Wang, Yushan Hu, Xiao Liu, Jing Tao, Gianni Sagratini, Qisen Xiang
Summary: This study reveals the antibacterial mechanism of lauric arginate (LAE) against Escherichia coli. LAE damages the cytoplasmic and outer membranes of E. coli, causing a loss of membrane integrity. It also induces oxidative stress and increases reactive oxygen species levels. The combination of membrane damage and oxidative stress contributes to the antibacterial activity of LAE.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Acoustics
Qiao He, Donghong Liu, Muthupandian Ashokkumar, Xingqian Ye, Tony Z. Jin, Mingming Guo
Summary: This study explored the response of bacterial cell membranes to ultrasound exposure. It was found that ultrasonic treatment resulted in a significant reduction in E. coli populations and increased sensitivity to antimicrobial compounds. Additionally, ultrasonic-treated cells showed membrane damages and increased membrane permeability.
ULTRASONICS SONOCHEMISTRY
(2021)
Review
Nanoscience & Nanotechnology
Yuqian Guo, Liang Guo, Yu Su, Yonghua Xiong
Summary: This review provides an overview of the CRISPR-Cas system as a molecular manipulation technology and nanomaterials as novel signal transducers for early cancer diagnosis. It summarizes the working mechanisms of the CRISPR-Cas system and the various signal models used for cancer diagnosis, and discusses the prospects and challenges of CRISPR-Cas-based nanosensors.
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Caicai Fu, Tianying Lu, Xiao Dai, Ping Ding, Yonghua Xiong, Jun Ge, Xiaoyang Li
Summary: In this study, a chemoenzymatic cascade reaction using a hybrid catalyst is proposed for the efficient and green removal of mycotoxins. The GOx-Fe3O4@COF hybrid catalyst, synthesized by compartmental co-immobilized glucose oxidase (GOx) and Fe3O4 nanoparticles (NPs) on a flower-shaped covalent organic framework (COF), exhibits excellent activity in mycotoxin removal. The degradation efficiency of aflatoxin B-1 (AFB(1)) is significantly higher in the chemoenzymatic cascade reaction, catalyzed by GOx-Fe3O4@COF, compared to the Fenton reaction catalyzed by Fe3O4@COF. The GOx-Fe3O4@COF hybrid catalyst shows high activity in a wide pH range of 3.0-7.0, overcoming the pH limitation of the Fenton reaction.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Food Science & Technology
Weipeng Tong, Hanpeng Xiong, Hao Fang, Yuhao Wu, Haichuan Li, Xiaolin Huang, Yuankui Leng, Yonghua Xiong
Summary: In order to achieve high-throughput analysis of mycotoxins, there is a great need for point-of-care (POC) methods that do not require expensive instruments or specialized technicians. The conventional enzyme-linked immunosorbent assay (ELISA), which is widely used for screening, has low sensitivity and is affected by competing antigens. In this study, we developed a plasmonic-photothermal ELISA that uses color-temperature dual-modal signals for highly sensitive detection of ochratoxin A (OTA), with the signal amplified by a bifunctional M13 phage. Under optimal conditions, the colorimetric and photothermal signals showed good dynamic linearity for quantitative OTA detection, with detection limits of 12.1 and 8.6 pg mL(-1), respectively. Additionally, the proposed ELISA could be visually determined with a cutoff limit of 78 pg mL(-1) based on a distinct color change from deep blue to red. The method also showed high accuracy and robustness when applied to OTA-spiked corn samples. Overall, this strategy provides a promising method for eco-friendly and sensitive POC screening of OTA, and can be easily adapted for other analytes by changing the specific mimotope sequence.
Article
Food Science & Technology
Hanpeng Xiong, Ping Chen, Xirui Chen, Xuanang Shen, Xiaolin Huang, Yonghua Xiong, Yu Su
Summary: In this study, a novel aggregation-induced emission (AIE)-based sandwich lateral flow immunoassay (LFIA) was introduced for the sensitive detection of Staphylococcal enterotoxin A (SEA) using red-emissive AIE nanoparticles (AIENPs) as the fluorescent nanoprobe. The ultrasensitive detection of SEA in pasteurised milk was achieved within 20 min with a limit of detection of 0.04 ng mL(-1). The proposed PBA-AIENP-LFIA platform shows promise as a potent tool for the identification of additional compounds in food samples as well as an ideal test method for on-site detections.
Article
Food Science & Technology
Ge Xu, Xiaojing Fan, Xirui Chen, Zilong Liu, Guoxin Chen, Xiaxia Wei, Xiangmin Li, Yuankui Leng, Yonghua Xiong, Xiaolin Huang
Summary: In this study, ultrabright fluorescent microbeads (AIEMBs) were developed by encapsulating a green-emitting fluorophore with aggregation-induced emission (AIE) characteristic in polymer nanoparticles. The AIEMBs were then used as signal reporters in a competitive LFIA for the highly sensitive screening of fumonisin B1 (FB1) in corn samples. The AIE-LFIA demonstrated high sensitivity, excellent selectivity, good accuracy, and high reliability, indicating a promising platform for FB1 screening.
Article
Chemistry, Physical
Gan Zhang, Ganggang Zhang, Xiaocui Lai, Liu Su, Weihua He, Weihua Lai, Shengliang Deng
Summary: In this study, the improved performance of lateral flow immunoassay (LFIA) was achieved by using aggregation-induced emission fluorescent microspheres (AIEFMs) with high-fluorescent property and a biofriendly antibody coupling strategy using a polydopamine (PDA) layer. The fluorescence intensity of AIEFMs was quenched by PDA through Förster resonance energy transfer (FRET), but quenching could be effectively controlled by adjusting the thickness of the PDA layer. The PDA-coated AIEFMs (AIEFM@PDA) not only retained the strong fluorescence of AIEFMs, but also improved the antibody coupling efficiency and reproducibility of LFIAs, making it suitable for highly sensitive detection of pathogenic bacteria (Escherichia coli O157:H7) and antibiotics (enrofloxacin).
CHEMISTRY OF MATERIALS
(2023)
Article
Agriculture, Multidisciplinary
Mengjun Zhou, Xirui Chen, Xuan-ang Shen, Xiangkai Lin, Ping Chen, Zhaohui Qiao, Xiangmin Li, Yonghua Xiong, Xiaolin Huang
Summary: Highly luminescent aggregation-induced emission luminogens embedded nanospheres (AIENPs) were introduced as signal amplification probes of lateral flow immunoassay (LFIA) for quantitative detection of ZEN. Compared with time-resolved dye embedded nanoparticles (TRNPs), AIENPs showed stronger photoluminescence intensity and superior environmental tolerance. AIENP-LFIA exhibited better dynamic linearity and lower detection limit than TRNP-LFIA, making it a practical method for rapid, sensitive, specific, and accurate quantitative detection of ZEN in corn samples.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Tianying Lu, Caicai Fu, Yonghua Xiong, Zheling Zeng, Yunkai Fan, Xiao Dai, Xiaolin Huang, Jun Ge, Xiaoyang Li
Summary: AFB1 contamination in edible oils is a significant concern for safety. Enzymatic degradation provides a promising method, but its efficiency is hindered by low enzyme activity and stability. In this study, an amphipathic laccase-inorganic hybrid nanoflower (Lac NF-P) was developed as a biocatalyst to degrade AFB1 in peanut oil. Lac NF-P exhibited 134- and 3.2-fold higher degradation efficiency compared to laccase and Lac NF, respectively. Within 3 hours, Lac NF-P reduced AFB1 concentration from 50-150 μg/kg to less than 0.96 μg/kg in peanut oil without affecting its quality. This study opens up new possibilities for developing biocatalysts to detoxify mycotoxins in edible oils.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Analytical
Wenchao Zhan, Yu Su, Xirui Chen, Hanpeng Xiong, Xiaxia Wei, Xiaolin Huang, Yonghua Xiong
Summary: A hydrogel probe encapsulating aggregation-induced emission luminogen (AIEgen) was developed for ultrasensitive detection of Hg2+, with improved sensitivity through the use of hydrophilic hydrogels and spatial networks. The probe exhibited high specificity, accuracy and precision in detecting Hg2+ in real-world water samples, with a sensitivity 4-fold higher than that of pure AIEgen probes. This TR hydrogel-based chemosensor holds promising potential as a robust, fast and effective platform for sensitive Hg2+ detection.
Article
Chemistry, Multidisciplinary
Hao Fang, Yaofeng Zhou, Yanbing Ma, Qi Chen, Weipeng Tong, Shengnan Zhan, Qian Guo, Yonghua Xiong, Ben Zhong Tang, Xiaolin Huang
Summary: This study presents a highly sensitive quantitative detection strategy for low-abundance target analytes by utilizing the synergistic combination of M13 phage-mediated leverage effect and GISG-amplified light scattering signal modulation. The strategy achieves ultrasensitive and rapid quantification of target analytes and displays about 4 orders of magnitude enhancement in sensitivity compared to traditional methods.
Article
Agriculture, Dairy & Animal Science
Xudong Jing, Shan Shan, Keyu Xing, Wenkai Cao, Xiaoyue Xiao, Daofeng Liu, Weihua Lai
Summary: Traditional ELISA is not sensitive enough for detecting E. coli O157:H7, but a new method utilizing streptavidin scaffolded DNA tetrad (SS-DNAt) and fluorescence poly adenine-thymine-templated copper nanoclusters shows improved sensitivity. This fluorescence ELISA has a linear range of 104 to 106 cfu/mL, with a detection limit of 3.75 x 10(3) cfu/mL, which is 6.16 times better than traditional ELISA. The method also shows good recovery rate (86.7% to 93.6%) and low coefficient of variation (5.6% to 10.5%) in milk. This method can be used for detecting hazardous materials in food.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Chemistry, Analytical
Qian Guo, Jun Huang, Hao Fang, Xiaoyang Li, Yu Su, Yonghua Xiong, Yuankui Leng, Xiaolin Huang
Summary: In this study, an ultrasensitive dynamic light scattering (DLS) immunosensor was proposed for the detection of Salmonella. The sensor utilized a cargo release-seed growth strategy enabled by gold nanoparticle-decorated covalent organic frameworks (COF@AuNP) to generate a significantly enhanced light-scattering signal, resulting in improved detection sensitivity. The immunosensor could detect trace amounts of Salmonella in milk in the range of 2.0 x 10(2)-2.0 x 10(5) CFU mL(-1), with a limit of detection of 60 CFU mL(-1). It also demonstrated excellent selectivity, accuracy, precision, and reliability for detecting Salmonella in milk.
Article
Chemistry, Multidisciplinary
Gan Zhang, Zhen Huang, Liwen Hu, Yumeng Wang, Shengliang Deng, Daofeng Liu, Juan Peng, Weihua Lai
Summary: This study developed a lateral flow immunoassay based on aggregation-induced emission nanoparticles for the sensitive detection of E. coli O157:H7. By integrating aggregation-induced emission luminogens into a nanostructured system, the detection of E. coli O157:H7 with colorimetric and fluorescent readout was achieved. The proposed method showed superior qualitative and quantitative abilities compared to other methods.
Article
Chemistry, Analytical
Yumeng Wang, Gan Zhang, Xiaoyue Xiao, Xinhui Shu, Dan Fei, Yelan Guang, Yaomin Zhou, Weihua Lai
Summary: This study demonstrates the use of aggregation-induced emission (AIE) luminogens encapsulated in polymers as labels to improve the sensitivity of lateral flow immunoassay (LFIA). The fluorescence resonance energy transfer mode was verified through theoretical calculation and spectral analysis. The labeled AIE polymer microspheres (DAIEPMs) exhibited a large Stokes shift and significantly enhanced fluorescence, enabling sensitive detection of chlorothalonil with a lower detection limit compared to traditional methods using quantum dot beads or gold nanoparticles.
ANALYTICAL CHEMISTRY
(2023)
