期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 67, 期 35, 页码 9831-9839出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b03939
关键词
exopolysaccharides; mucosal homeostasis; epithelial cell differentiation; Muc2; c-Jun
资金
- National Natural Science Foundation of China for Distinguished Young Scholars [31825020]
- National Key Technology R&D Program of China [2012BAD33B06]
- Natural Science Foundation of Jiangxi Province [20192BAB204024]
- Outstanding Science and Technology Innovation Team Project in Jiangxi Province [2016RCYTB0030]
Probiotic lactobacilli and their exopolysaccharides (EPS) are thought to modulate mucosal homeostasis; however, their mechanisms remain elusive. Thus, we tried to clarify the role of exopolysaccharides from Lactobacillus plantarum NCU116 (EPS116) in the intestinal mucosal homeostasis. Our results indicated that EPS116 regulated the colon mucosal healing and homeostasis, enhanced the goblet cell differentiation, and promoted the expression of Muc2 gene in vivo and in vitro. Further experiments showed that EPS116 promoted the expression and phosphorylation of transcription factor c-Jun and facilitated its binding to the promoter of Muc2. Moreover, knocking down c-Jun or inhibiting its function in LS 174T cells treated with EPS116 led to decreased expression of Muc2, implying that EPS116 promoted the colonic mucosal homeostasis and Muc2 expression via c-Jun. Therefore, our study uncovered a novel model where EPS116 enhanced colon mucosal homeostasis by controlling the epithelial cell differentiation and c-Jun/Muc2 signaling.
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