Article
Microbiology
Marcela Manduca Ferreira, Marina Lopes Mechler-Dreibi, Karina Sonalio, Henrique Meiroz de Souza Almeida, Maria Eugenia Silveira Ferraz, Ana Paula Prudente Jacintho, Dominiek Maes, Luis Guilherme de Oliveira
Summary: In this study, Mycoplasma infections in slaughter pigs in Brazil were quantified, revealing correlations between the degree of infection with Mycoplasma hyopneumoniae and Mycoplasma flocculare and the severity of macroscopic lung consolidation lesions. Co-infections of Mycoplasma flocculare and Mycoplasma hyorhinis were also found to be positively correlated with the extent of lung lesions. Additionally, Mycoplasma hyopneumoniae exhibited inhibitory behavior towards Mycoplasma flocculare, suggesting a competitive relationship between them.
VETERINARY MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Thomas Ipoutcha, Geraldine Gourgues, Carole Lartigue, Alain Blanchard, Pascal Sirand-Pugnet
Summary: Mycoplasma gallisepticum (Mgal) is a common poultry pathogen that spread to North American house finches after a host shift in 1994. The molecular determinants of Mgal virulence and host specificity are not well understood due to the lack of efficient methods for functional genomics. This study successfully used a recombination system derived from Bacillus subtilis for gene inactivation and targeted replacement in Mgal, providing the first genetic tool for targeted genome engineering of Mgal.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Microbiology
Kohei Kobayashi, Noriyuki Kodera, Taishi Kasai, Yuhei O. Tahara, Takuma Toyonaga, Masaki Mizutani, Ikuko Fujiwara, Toshio Ando, Makoto Miyata
Summary: This study visualized the real-time movements of the internal structure of Mycoplasma mobile using high-speed atomic force microscopy, revealing a connection between movement and ATP hydrolysis. The internal structure's behavior was analyzed when partially inhibited by sodium azide, showing specific movements and patterns related to gliding on solid surfaces.
Article
Microbiology
Arthur H. Totten, Anna Julia Adams, Hyunmi K. Halas, James E. T. Gebo, Amanda D. East, Anna F. Lau
Summary: Testing of cellular therapy products for Mycoplasma is required by the FDA to ensure sterility and safety. Various molecular assays have been marketed for faster testing, but their performance is not well-documented. This study evaluated the performance of five commercial molecular assays and found that not all assays met the LOD required by pharmacopeia standards.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Microbiology
M. Mizutani, Y. Sasajima, M. Miyata
Summary: The study measured the stall force of Mycoplasma pneumoniae cells and found M129 and FH strains have different binding activity and gliding speed on sialylated oligosaccharides, showing that binding activity is not linked to gliding force. The presence of a drag force during gliding was indicated by the decrease in gliding speed when cell binding was reduced. Stepwise movements were detected, likely caused by a single leg under certain conditions, and the step size and force applied on a leg were investigated in relation to the free energy of ATP hydrolysis.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Alannah S. Deeney, Rachael Collins, Anne M. Ridley
Summary: The study found that Mycoplasma bovis remains the most common species in farmed ruminants in England and Wales, particularly associated with respiratory diseases in cattle. The prevalence of Mycoplasma ovipneumoniae has increased in recent years, especially in coughing lambs, and should be considered a primary differential diagnosis for respiratory diseases in small ruminants.
BMC VETERINARY RESEARCH
(2021)
Review
Microbiology
Ken B. Waites, Donna M. Crabb, Amy E. Ratliff, William M. Geisler, T. Prescott Atkinson, Li Xiao
Summary: Mycoplasma genitalium is a sexually transmitted pathogen that affects both men and women. Molecular-based diagnostic tests are needed due to its slow growth and demanding culture requirements. The availability of FDA-cleared commercial molecular-based assays has expanded diagnostic testing in the United States, leading to updated guidelines for testing and treatment. This review summarizes the importance of M. genitalium as a human pathogen, explains the need for microbiological diagnosis, describes available diagnostic methods, and discusses the impact of antimicrobial resistance on treatment options.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Dermatology
Michael Arabatzis, Aristea Velegraki
Summary: This report describes the detection of an oral Tenericutes species in the skin lesions of a patient with psoriasis, raising new questions about the pathogenesis of the disease. The presence of wall-less bacteria intracellularly and extracellularly in the psoriatic skin suggests a potential link to the autoimmune/autoinflammatory nature of psoriasis.
JOURNAL OF CUTANEOUS PATHOLOGY
(2022)
Article
Immunology
Prakhar Vijayvargiya, Zerelda Esquer Garrigos, Cassie C. Kennedy, Richard C. Daly, Mark E. Wylam, Robin Patel, Elena Beam
Summary: This study evaluated pretransplant screening for Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum in lung transplant recipients and found that donor-derived infections were the main issue. Preemptive therapy did not significantly impact the overall incidence of infection.
OPEN FORUM INFECTIOUS DISEASES
(2022)
Review
Immunology
Ourlad Alzeus G. Tantengco, Mariana de Castro Silva, Clarissa L. Velayo
Summary: Our meta-analysis revealed a significant association between M. genitalium, M. hominis, and U. urealyticum infections and female infertility. This evidence supports the need for guidelines for the diagnosis and treatment of genital mycoplasma infections to prevent female infertility.
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ana S. Ramirez, Jose B. Poveda, Remco Dijkman, Carlos Poveda, Alejandro Suarez-Perez, Anneke Feberwee, Michael P. Szostak, Lorenzo Ressel, Ruben S. Rosales, Tomeu Viver, Pascual Calabuig, Salvatore Catania, Federica Gobbo, Dorina Timofte, Joachim Spergser
Summary: In this study, six Mycoplasma strains were isolated from tracheal swabs of wild seabirds, with one strain showing high similarity to Mycoplasma gallisepticum. Phylogenetic analysis of marker genes further confirmed the close relationship of these strains to M. gallisepticum. The strains grew well in culture and exhibited typical colony and cell morphology.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Natalia C. Gaeta, Ana Marcia de Sa Guimaraes, Jorge Timenetsky, Stephanie Clouser, Lilian Gregory, Erika Ganda
Summary: This study provides the first description of M. ovipneumoniae in Brazilian sheep, revealing the genomic characteristics of the strain and its relatedness to isolates from other countries. This highlights the importance of continuous surveillance and diagnostics of pathogens causing respiratory disease in sheep in Brazil.
VETERINARY RESEARCH COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
Ana Cutoiu, Daniel Boda
Summary: The aim of this study was to assess the antimicrobial susceptibility and resistance to antibiotics against Ureaplasma urealyticum and Mycoplasma hominis genital isolates in Romania. Samples were collected from patients at Ponderas Academic Hospital in Bucharest from January to December 2021. The study found high rates of U. urealyticum genital infection in men and a significant association between U. urealyticum and M. hominis in women. The isolates showed susceptibility to doxycycline and tetracycline but resistance to quinolones.
Article
Agronomy
Barbara Souza Neil Magalhaes, Leandro dos Santos Machado, Arthur de Almeida Figueira, Thomas Salles Dias, Thiago de Almeida Feijo, Maria Lucia Barreto, Giulia de Almeida Tuffanelli, Nathalie Costa da Cunha, Elmiro Rosendo do Nascimento, Virginia Leo de Almeida Pereira, Nadia Regina Pereira Almosny
Summary: This study investigated the occurrence of Mycoplasma species in captive snakes from RioZoo, Brazil, and found a 19.23% positive rate among the 26 snakes tested. Mycoplasma spp. were detected in snakes with and without clinical signs, showing similarity to those described in other reptiles. No relationship was found between the presence of Mycoplasma spp. and clinical signs.
Article
Microbiology
Daiki Matsuike, Yuhei O. Tahara, Takahiro Nonaka, Heng Ning Wu, Tasuku Hamaguchi, Hisashi Kudo, Yuuki Hayashi, Munehito Arai, Makoto Miyata
Summary: In this study, the function and structure of the surface protein Gli123 in Mycoplasma mobile were investigated. It was found that Gli123 has two distinct globular and rod-like structures, and it plays a crucial role in the assembly of the surface gliding machinery through the evolution of repetitive lipoprotein units.
JOURNAL OF BACTERIOLOGY
(2023)
Article
Infectious Diseases
Elisabetta Colosi, Giulia Bassignana, Alain Barratt, Bruno Lina, Philippe Vanhems, Julia Bielicki, Vittoria Colizza
Summary: As the Omicron variant cases increased in early 2022, schools faced significant disruptions. A study compared reactive screening, regular screening, and reactive class closure protocols in France, Baselland (Switzerland), and Italy, respectively. The findings showed that reactive screening required more testing resources but had lower control compared to weekly screening. The study suggests optimizing school protocols based on incidence levels. Evaluation: 9 out of 10.
Article
Multidisciplinary Sciences
Jonathan Roux, Clement R. Massonnaud, Vittoria Colizza, Simon Cauchemez, Pascal Crepey
Summary: We assessed the impact of national and regional lockdowns considering the French first epidemic wave of COVID-19 as a case study. Regional lockdowns for preventing ICU saturation would have been needed in almost all French regions within 10 days. The timing of lockdowns, whether at a regional or national level, significantly affects the morbidity, mortality, and stress on the healthcare system.
SCIENTIFIC REPORTS
(2023)
Article
Agriculture, Dairy & Animal Science
Francisco Rodriguez, Ruben S. Rosales, Ana S. Ramirez, Jose B. Poveda
Summary: Mycoplasma hyopneumoniae is the causative agent of enzootic pneumonia in pigs, leading to impaired immune-inflammatory function and fatal pneumonia. Vaccination is commonly used, but its effectiveness varies. Enhancing Th1 response is crucial to improve vaccine efficacy.
Review
Plant Sciences
Alexandra J. Weisberg, Yu wu, Jeff H. Chang, Erh-Min Lai, Chih-Horng Kuo
Summary: Agrobacteria are feared as agricultural pathogens, causing abnormalities and economic damage to plants, but they are also revered in the laboratory for their usefulness as models and tools in biological research. This review synthesizes elegant work on agrobacterial virulence mechanisms and ecology, emphasizing their genomic and plasmid characteristics, and highlights areas for further research.
ANNUAL REVIEW OF PHYTOPATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yi-Hsin Liu, Tong-Seung Tseng, Ching-Rong Wu, Shu-Ting Cho, Chih-Horng Kuo, Xin-Jie Huang, Jung-Yi Cheng, Kuo-Hsuan Hsu, Kung-Fu Lin, Chia-Chin Liu, Ching-Hui Yeh
Summary: The small heat shock proteins (sHSPs) OsHsp16.9A and OsHsp18.0 have been found to accumulate in response to heat shock and play different roles in thermotolerance in rice. Mass spectrometry analysis revealed that OsHsp16.9A forms a stable complex with OsHsp101, and specific amino acid residues of OsHsp16.9A are essential for this interaction. The interaction between OsHsp16.9A and OsHsp101 is crucial for enhancing thermotolerance in rice plants.
Article
Microbiology
Si-Chong Wang, Ai-Ping Chen, Shu-Jen Chou, Chih-Horng Kuo, Erh-Min Lai
Summary: The T6SS is used by proteobacteria to secrete effector proteins and influence bacterial competition or pathogenesis. In this study, the researchers demonstrated that the T6SS affects disease occurrence and the composition of the microbial community in crown galls.
Article
Agronomy
Shih-Hsun Walter Hung, Tsai-Ching Huang, Yu-Chih Lai, I-Chen Wu, Chia-Ho Liu, Yuh-Feng Huarng, Hau-Hsuan Hwang, En-Pei Isabel Chiang, Chih-Horng Kuo, Chieh-Chen Huang
Summary: Using Burkholderia seminalis 869T2 as a biostimulant can enhance the growth and quality of leafy vegetables, reduce fertilizer usage, and decrease costs while increasing product value.
Article
Microbiology
Yalu Li, Xiao-Hua Yan, Yanzhi Liu, Shen-Chian Pei, Chih-Horng Kuo, Weijie Huang
Summary: This work presents the complete genome sequence of Candidatus Phytoplasma aurantifolia TB2022, a bacterium associated with sweet potato little leaf disease in Fujian Province, China. The genome consists of one 670,073-bp circular chromosome.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Microbiology
Xiao-Hua Yan, Jixiu Lin, Yanzhi Liu, Peizhi Huang, Jianmi Liu, Qibin Hu, Yalu Li, Shen-Chian Pei, Weijie Huang, Chih-Horng Kuo
Summary: The complete genome sequence of Candidatus Phytoplasma asteris QS2022, a bacterium associated with lettuce chlorotic leaf rot disease in Fujian Province, China, is presented in this study. The genome consists of a single 834,303-bp circular chromosome.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Agriculture, Dairy & Animal Science
Marcos Fumero-Hernandez, Mario Encinoso, Ana Sofia Ramirez, Inmaculada Morales, Alejandro Suarez Perez, Jose Raduan Jaber
Summary: Advanced imaging techniques, such as CT, are valuable in providing detailed information on the ocular structures of seabirds, which require specific adaptations for their diverse aquatic, terrestrial, and aerial environments. Understanding the ocular anatomy and biology of these animals is important for clinicians, biologists, and researchers. However, there is a lack of publications regarding the use of imaging diagnosis in avian ophthalmology. Our study focused on the morphometric analysis of the ocular bulb and its internal structures in puffins using advanced imaging techniques such as CT.
Article
Veterinary Sciences
Ana S. Ramirez, Jose Raduan Jaber, Ruben S. Rosales, Magnolia Conde-Felipe, Francisco Rodriguez, Juan Alberto Corbera, Alejandro Suarez-Perez, Mario Encinoso, Ana Muniesa
Summary: The respect of teachers for their students is crucial for effective teaching, even more important than their knowledge and communication capacity. This article presents findings from online surveys, highlighting the need for teachers to analyze and question their attitudes towards students.
VETERINARY SCIENCES
(2023)
Article
Microbiology
Shen-Chian Pei, Ai-Ping Chen, Shu-Jen Chou, Ting-Hsuan Hung, Chih-Horng Kuo
Summary: This study presents the complete genome sequence of Candidatus Phytoplasma pruni strain PR2021, which includes one 705,138 bp circular chromosome and one 4,757 bp circular plasmid, and is associated with the poinsettia cultivar Princettia Pink.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Microbiology
John Raymond B. Salazar, Mary Nia M. Santos, Jeremy L. Palaad, Hsi-Ching Yen, Jose Louis A. Castellano, Leobert D. dela Pena, Edgar C. Amar, Erh-Min Lai, Chih-Horng Kuo
Summary: This study reports the complete genome sequence of Vibrio parahaemolyticus strain PH1273 collected from a Penaeus vannamei pond in the Philippines in 2015. Genome analysis revealed the absence of the pirAB gene responsible for causing acute hepatopancreatic necrosis disease, but the presence of multiple secretion systems and their associated effectors.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Microbiology
Shu-Ting Cho, Ai-Ping Chen, Shu-Jen Chou, Xiao-Hua Yan, Chan-Pin Lin, Chih-Horng Kuo
Summary: This study presents the complete genome sequence of Candidatus Phytoplasma cynodontis strain GY2015, which is an uncultivated plant-pathogenic bacterium associated with Bermuda grass white leaf disease in Taoyuan, Taiwan.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Microbiology
Shih-Hsun Walter Hung, I-Chen Wu, Chieh-Chen Huang, Chih-Horng Kuo
Summary: This study reports the isolation and genome sequence of a coral-associated Erythrobacteraceae bacterium, strain WH01K, which consists of a circular chromosome of 2,745,896 bp and a circular plasmid of 172,502 bp.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
