Article
Chemistry, Analytical
Maria Carcelen, Veronica Vidal, Alfredo Franco, Marcos Gomez, Fernando Moreno, Jose L. Fernandez-Luna
Summary: This article introduces a simple, fast and label-free method based on a gold nanohole array biosensor to detect the spectral response and changes in cell-matrix adhesion of cancer cells. The experimental results show that this biosensor can effectively distinguish different cell populations in the cell cycle and detect changes in cell-matrix interaction in real time.
Article
Oncology
Malwina Sosnowska, Marta Kutwin, Barbara Strojny, Piotr Koczon, Jaroslaw Szczepaniak, Jasmina Balaban, Karolina Daniluk, Slawomir Jaworski, Andre Chwalibog, Wieslaw Bielawski, Ewa Sawosz
Summary: The study demonstrates that modifying the physical characteristics of the ECM with graphene oxide and enriching the cell environment with a mixture of natural proteins can reduce the invasiveness of liver cancer cells by increasing adhesion through integrin expression.
CANCER NANOTECHNOLOGY
(2021)
Article
Cell Biology
Anna Germon, Kate J. Heesom, Reiss Amoah, Josephine C. Adams
Summary: PDIA3 is an isomerase of the endoplasmic reticulum that is correlated with aggressive breast cancers. Inhibition of PDIA3 reduces the effects of promigratory factors secreted by MDA-MB-231 breast cancer cells on newly adherent cells, suggesting that PDIA3 may be a potential therapeutic target for basal breast cancer.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Editorial Material
Chemistry, Multidisciplinary
David M. Dolivo, Adrian E. Rodrigues, Thomas A. Mustoe, Robert D. Galiano, Seok Jong Hong
Summary: Cellular therapies hold promise for fibrosis treatment. A recent article presents a strategy and proof-of-concept for delivering stimulated cells to degrade hepatic collagen in vivo. The discussion highlights the strengths of this approach and its potential to generalize to treat other forms of fibrosis.
Review
Cell Biology
Andrea Ravasio, Eugenia Morselli, Cristina Bertocchi
Summary: Cells respond to mechanical forces and physical cues from their environment, with autophagy playing a major role in maintaining cellular processes and regulating interactions with the environment. This review summarizes the interaction between autophagy and mechanotransduction machinery associated with cell adhesions, termed as "Mechanoautophagy".
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Ting Zhang, Xianyu Huang, Sujun Liu, Xinyue Bai, Xinyue Zhu, Dennis O. Clegg, Mei Jiang, Xiaodong Sun
Summary: This study investigated the cryopreservation of hESC-derived RPE cells and found that freezing the cells at passage 2 day 5 (P2D5) resulted in the best post-thawing outcome in terms of cell viability and preservation of RPE cell properties and functions. The RNA-sequencing analysis identified key pathways and molecules associated with RPE cell freezing tolerance. The findings provide valuable insights for further research in this area.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Oncology
Hongjun Xiao, Yezhen Zhang, Zhaofeng Li, Bin Liu, Dan Cui, Fan Liu, Dafan Chen, Yingfu Liu, Gaoliang Ouyang
Summary: Periostin plays a critical role in the pathogenesis of liver disorders and the development of HCC. This study demonstrates that HSC-derived periostin promotes HCC development by enhancing HSC activation through multiple pathways and by augmenting HCC cell proliferation via the ERK pathway.
JOURNAL OF PATHOLOGY
(2021)
Article
Gastroenterology & Hepatology
Ray-Hwang Yuan, Chia-Lang Hsu, Yu-Lin Jhuang, Yun-Ru Liu, Tsung-Han Hsieh, Yung-Ming Jeng
Summary: The interaction between liver cancer cells and the extracellular matrix can induce cholangiocytic differentiation and switch the behavior of liver cancer cells from a proliferative to an invasive phenotype. This partially explains the differences in behavior between hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC).
HEPATOLOGY INTERNATIONAL
(2022)
Article
Cell Biology
Zhiling Zhou, Jiaxin Zhan, Qiong Luo, Xinghua Hou, Shuxia Wang, Dingzhang Xiao, Zhi Xie, Haidan Liang, Shuguang Lin, Meng Zheng
Summary: ADRB3 plays a crucial role in the migration and differentiation of tumor cells and immune cells, and is associated with the malignancy, TNM stage, and prognosis of breast cancer. It promotes the expansion of MDSCs, inhibits the differentiation of tumor cells, and enhances cell proliferation through activation of the mTOR pathway.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Eun-Ju Lee, Khurshid Ahmad, Shiva Pathak, SunJu Lee, Mohammad Hassan Baig, Jee-Heon Jeong, Kyung-Oh Doh, Dong-Mok Lee, Inho Choi
Summary: This study introduces novel peptides derived from fibronectin that enhance cell adhesion, proliferation, and differentiation, offering a new approach to cell culture. The peptides showed significant effects on primary cells and stem cells, increasing proliferation and promoting differentiation into specific cell types such as osteoblasts. The results suggest potential applications in the production of core materials for cell culture such as scaffolds and culture medium components.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Xiaochen Wang, Shaohua Chen, Hanqing Nan, Ruchuan Liu, Yu Ding, Kena Song, Jianwei Shuai, Qihui Fan, Yu Zheng, Fangfu Ye, Yang Jiao, Liyu Liu
Summary: Research on pattern formation in coculture cell systems reveals that invasive cells exhibit centripetal motion and abnormal aggregation, rather than random spreading, due to a collective pulling effect mediated by ECM. This mechanism may provide new insights into biological processes involving multiple cell types.
Article
Biochemistry & Molecular Biology
Jayashree Sahana, Jose Luis Cortes-Sanchez, Viviann Sandt, Daniela Melnik, Thomas J. Corydon, Herbert Schulz, Zexi Cai, Katja Evert, Daniela Grimm, Markus Wehland
Summary: This study cultured breast cancer cell lines MCF-7 and MDA-MB-231 under simulated microgravity conditions and investigated the gene expression pattern and cellular signaling pathways involved. The results showed that the engineered three-dimensional multicellular spheroid model can be used to study breast cancer cell behavior and evaluate the efficacy of drugs against breast cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Ellen Emi Kato, Luciana Araujo Pimenta, Maira Estanislau Soares de Almeida, Vanessa Olzon Zambelli, Marinilce Fagundes dos Santos, Sandra Coccuzzo Sampaio
Summary: The study demonstrated that CTX inhibits major events involved in angiogenesis, such as tube formation and cell adhesion, proliferation, and migration. These actions were particularly effective against tumor stimuli, highlighting the importance of CTX's anti-angiogenic action in inhibiting tumor progression.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Cell Biology
Amanda Haage, Archana Dhasarathy
Summary: Cells are able to sense environmental changes, transmit signals, and influence adaptive changes in cell behavior. Mechanosensing, involving various macromolecular structures, plays a role in integrating physical stimuli and regulating cellular behavior. The extracellular matrix and cell adhesion proteins are crucial components of the cellular microenvironment, and their disruption can lead to disease. Recent advances in microscopy and genomic technologies have enhanced our understanding of the impact of nuclear localization of adhesion proteins. This review will cover the migration of adhesion proteins to the nucleus and their downstream functions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Chemistry, Analytical
Xiaomin Wu, Ri Liu, Li Li, Fan Yang, Dongdong Liu, Lu Wang, Wentao Yu, Junyang Xu, Zhankun Weng, Litong Dong, Zuobin Wang
Summary: This paper investigates the effect of physically modified silicon nanostructures on single-cell patterning. It is found that physically modified grid silicon nanostructures can regulate the single-cell localization growth, and the rational design of substrate structures can maximize the single-cell localization ratio.
ANALYTICAL METHODS
(2022)
Article
Biochemistry & Molecular Biology
Aleksandra Kalinska, Slawomir Jaworski, Mateusz Wierzbicki, Magdalena Kot, Daniel Radzikowski, Sebastian Smulski, Marcin Golebiewski
Summary: This paper aims to evaluate the influence of common cosmetic substrates and mixtures of nanoparticles on the viability of mastitis pathogens. The results show that only collagen + elastin and glycerine increased bacterial viability, while the rest of the cosmetic substrates decreased it. Pre-dipping and dipping mixtures significantly decreased the viability of the mentioned pathogens. These promising results suggest that further testing in different herd conditions is needed to assess their efficacy in mastitis prevention or reduction. Additionally, analysis on the influence of these mixtures on other bacteria species, algae, and fungi is necessary.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nanoscience & Nanotechnology
Jasmina Balaban, Mateusz Wierzbicki, Marlena Zielinska-Gorska, Malwina Sosnowska, Karolina Daniluk, Slawomir Jaworski, Piotr Koczon, Dominik Cysewski, Andre Chwalibog, Ewa Sawosz
Summary: This experiment aimed to prove the existence of the ACE2 receptor on human muscle cells and investigate the impact of the interaction between these cells and the SARS-CoV-2 virus spike protein. The experiments confirmed the presence of ACE2 receptor in human skeletal muscle cells and showed that the spike protein of the SARS-CoV-2 virus influences the activation of pro-inflammatory proteins. The use of graphene oxide at low levels does not have adverse effects on muscle cells and reduces the levels of most proteins, including pro-inflammatory proteins.
NANOTECHNOLOGY SCIENCE AND APPLICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Karolina Daniluk, Agata Lange, Barbara Wojcik, Katarzyna Zawadzka, Jasmina Balaban, Marta Kutwin, Slawomir Jaworski
Summary: This research explores the use of graphene nanomaterials as carriers for bee venom component melittin (M) to target breast cancer cells. The results indicate that M complexes with nanomaterials can effectively reduce oxidative stress levels compared to M alone, but using graphene as the carrier causes more severe DNA damage. Moreover, the use of M and graphene complex shows a greater inhibitory effect on tumor progression than a complex with graphene oxide.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Magdalena Kot, Aleksandra Kalinska, Slawomir Jaworski, Mateusz Wierzbicki, Sebastian Smulski, Marcin Golebiewski
Summary: Digital dermatitis (DD) is a common disease in dairy cattle, causing losses and negative impacts on welfare and milk yield. The etiology of DD is not fully understood and there is limited data available. Antibiotic therapy for disease management has led to antibiotic resistance, reducing effectiveness. This study investigates the biocidal properties of metal nanoparticles (NPs) as an alternative to antibiotics for pathogens causing hoof diseases in cows, particularly DD. AgNPs and CuNPs showed the highest biocidal effect on the isolated pathogens, suggesting their potential use in the treatment and prevention of lameness in cows.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nanoscience & Nanotechnology
Mateusz Wierzbicki, Katarzyna Zawadzka, Barbara Wojcik, Slawomir Jaworski, Barbara Strojny, Agnieszka Ostrowska, Artur Malolepszy, Marta Mazurkiewicz-Pawlicka, Ewa Sawosz
Summary: Diamond nanoparticles show varying toxicity to different cell types, and our study focuses on the specific sensitivity of endothelial cells. We characterized the diamond nanoparticles and evaluated their toxicity on endothelial cells, as well as their effects on various cellular processes and protein synthesis.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Kinga Zelechowska-Matysiak, Kamil Wawrowicz, Mateusz Wierzbicki, Tadeusz Budlewski, Aleksander Bilewicz, Agnieszka Majkowska-Pilip
Summary: In this study, a novel bioconjugate (DOX-AuNPs-Tmab) consisting of gold nanoparticles (AuNPs) attached to chemotherapeutic agent doxorubicin (DOX) and a monoclonal antibody, trastuzumab (Tmab), was synthesized. The bioconjugate exhibited specific binding to HER2 receptors and demonstrated high specificity of binding and internalization capabilities to HER2-positive cancer cells. Cytotoxicity experiments showed that DOX-AuNPs-Tmab significantly reduced cancer cell metabolic activity and surface area of spheroids, inducing cell cycle arrest and late apoptosis. These findings suggest that DOX-AuNPs-Tmab has great potential for targeted therapy of HER2-positive tumors.
Article
Nanoscience & Nanotechnology
Barbara Wojcik, Katarzyna Zawadzka, Slawomir Jaworski, Marta Kutwin, Malwina Sosnowska, Agnieszka Ostrowska, Marta Grodzik, Artur Malolepszy, Marta Mazurkiewicz-Pawlicka, Mateusz Wierzbicki
Summary: This study evaluated the cytotoxicity of 13 types of diamond nanoparticles with different production methods, sizes, and surface functional groups on tumor cell lines and a non-tumor cell line. The results showed that diamond nanoparticles had no toxicity on non-tumor cells but moderate toxicity on glioblastoma cell lines. The production method did not affect the toxicity, but modifications of the nanoparticles had varying effects on cell response.
Article
Nanoscience & Nanotechnology
Marta Kutwin, Malwina Sosnowska, Agnieszka Ostrowska, Maciej Trzaskowski, Agata Lange, Mateusz Wierzbicki, Slawomir Jaworski
Summary: This study investigated the effect of graphene oxide (GO) complexed with antisense miRNA-21 on cytokine expression levels in U87, U118, U251, and T98 glioma cell lines. The results showed that GO-antisense miRNA-21 complexes significantly regulated the expression levels of TIMP-2, IL-6, IL-8, ICAM-1, and MCP-1 compared to electroporation of antisense miRNA-21. Furthermore, GO-mediated antisense miRNA-21 transfection resulted in upregulation of TIMP-2 and downregulation of ICAM-1, as well as significant decrease in IL-8 expression level compared to electroporation.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Izabela Betlej, Boguslaw Andres, Tomasz Cebulak, Ireneusz Kapusta, Maciej Balawejder, Slawomir Jaworski, Agata Lange, Marta Kutwin, Elzbieta Pisulewska, Agnieszka Kidacka, Barbara Krochmal-Marczak, Piotr Borysiuk
Summary: This study assessed the qualitative and quantitative evaluation of bioactive compounds in ethanol extracts from leaves and flowers of Lavandula angustifolia Mill. grown in southern Poland. The antimicrobial properties, antioxidant properties, and composition of volatile substances were also examined. The results showed that the extracts significantly inhibited the viability of bacterial cells and the development of mold fungi. Leaf extracts had higher levels of polyphenols and antioxidant properties compared to flower extracts. The composition of volatile substances was also found to be different between the extracts.
Article
Biochemistry & Molecular Biology
Daniel Radzikowski, Aleksandra Kalinska, Magdalena Kot, Slawomir Jaworski, Mateusz Wierzbicki, Marcin Golebiewski
Summary: Prevention and treatment of mastitis in dry cows is a complex issue, and current intramammary antibiotic treatment varies in effectiveness. This study evaluates the practical use of metal nanoparticles in treating cows during their dry period, as these nanoparticles have strong biocidal properties against bacteria, fungi, and algae. The results show that certain nanoparticles and their complexes have biocidal properties against mastitis pathogens, indicating their potential as alternatives to antibiotics.
Article
Chemistry, Multidisciplinary
Kamil Wawrowicz, Kinga Zelechowska-Matysiak, Agnieszka Majkowska-Pilip, Mateusz Wierzbicki, Aleksander Bilewicz
Summary: Convenient therapeutic protocols against hepatocellular carcinoma (HCC) exhibit low treatment effectiveness, especially in the context of long-term effects, which is primarily related to late diagnosis and high tumor heterogeneity. Current trends in medicine concern combined therapy to achieve new powerful tools against the most aggressive diseases. When designing modern, multimodal therapeutics, it is necessary to look for alternative routes of specific drug delivery to the cell, its selective (with respect to the tumor) activity and multidirectional action, enhancing the therapeutic effect.
NANOSCALE ADVANCES
(2023)