Highly Efficient and Heritable Targeted Mutagenesis in Wheat via the Agrobacterium tumefaciens-Mediated CRISPR/Cas9 System

CRISPR/Cas9-mediated knockout of Ms1 enables the rapid generation of male-sterile hexaploid wheat lines for use in hybrid seed production

(2019) Anzu Okada et al.   PLANT BIOTECHNOLOGY JOURNAL

Development of an Agrobacterium -delivered CRISPR/Cas9 system for wheat genome editing

(2019) Zhengzhi Zhang et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR/Cas9-mediated targeted mutagenesis of GmSPL9 genes alters plant architecture in soybean

(2019) Aili Bao et al.   BMC PLANT BIOLOGY

Analysis of the functions of TaGW2 homoeologs in wheat grain weight and protein content traits

(2018) Yi Zhang et al.   PLANT JOURNAL

Concurrent modifications in the three homeologs of Ms45 gene with CRISPR-Cas9 lead to rapid generation of male sterile bread wheat (Triticum aestivum L.)

(2018) Manjit Singh et al.   PLANT MOLECULAR BIOLOGY

Chromatin accessibility is associated with CRISPR-Cas9 efficiency in the zebrafish (Danio rerio)

(2018) Meri I. E. Uusi-Mäkelä et al.   PLoS One

Gene editing and mutagenesis reveal inter-cultivar differences and additivity in the contribution of TaGW2 homoeologues to grain size and weight in wheat

(2018) Wei Wang et al.   THEORETICAL AND APPLIED GENETICS

Biolistic-delivery-based transient CRISPR/Cas9 expression enables in planta genome editing in wheat

(2018) Haruyasu Hamada et al.   Scientific Reports

Targeted mutagenesis using the Agrobacterium tumefaciens-mediated CRISPR-Cas9 system in common wheat

(2018) Shujuan Zhang et al.   BMC PLANT BIOLOGY

Efficient generation of stable, heritable gene edits in wheat using CRISPR/Cas9

(2018) Rhian M Howells et al.   BMC PLANT BIOLOGY

Hi-TOM: a platform for high-throughput tracking of mutations induced by CRISPR/Cas systems

(2018) Qing Liu et al.   Science China-Life Sciences

Heterochromatin delays CRISPR-Cas9 mutagenesis but does not influence the outcome of mutagenic DNA repair

(2018) Eirini M. Kallimasioti-Pazi et al.   PLOS BIOLOGY

CRISPR/Cas9 genome editing in wheat

(2017) Dongjin Kim et al.   FUNCTIONAL & INTEGRATIVE GENOMICS

Low-gluten, nontransgenic wheat engineered with CRISPR/Cas9

(2017) Susana Sánchez-León et al.   PLANT BIOTECHNOLOGY JOURNAL

Selective gene dosage by CRISPR-Cas9 genome editing in hexaploid Camelina sativa

(2017) Céline Morineau et al.   PLANT BIOTECHNOLOGY JOURNAL

Simultaneous modification of three homoeologs of TaEDR1 by genome editing enhances powdery mildew resistance in wheat

(2017) Yunwei Zhang et al.   PLANT JOURNAL

Characteristics of Genome Editing Mutations in Cereal Crops

(2017) Changfu Zhu et al.   TRENDS IN PLANT SCIENCE

Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes

(2017) Zhen Liang et al.   Nature Communications

MS26/CYP704B is required for anther and pollen wall development in bread wheat (Triticum aestivum L.) and combining mutations in all three homeologs causes male sterility

(2017) Manjit Singh et al.   PLoS One

Efficiency and Inheritance of Targeted Mutagenesis in Maize Using CRISPR-Cas9

(2016) Jinjie Zhu et al.   Journal of Genetics and Genomics

Efficient Targeted Genome Modification in Maize Using CRISPR/Cas9 System

(2016) Chao Feng et al.   Journal of Genetics and Genomics

AnAgrobacterium-delivered CRISPR/Cas9 system for high-frequency targeted mutagenesis in maize

(2016) Si Nian Char et al.   PLANT BIOTECHNOLOGY JOURNAL

Efficient CRISPR/Cas9-based gene knockout in watermelon

(2016) Shouwei Tian et al.   PLANT CELL REPORTS

The Impact of Chromatin Dynamics on Cas9-Mediated Genome Editing in Human Cells

(2016) René M. Daer et al.   ACS Synthetic Biology

A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants

(2015) Xingliang Ma et al.   Molecular Plant

Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system

(2015) Kabin Xie et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Generation of inheritable and “transgene clean” targeted genome-modified rice in later generations using the CRISPR/Cas9 system

(2015) Rong-Fang Xu et al.   Scientific Reports

Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation

(2015) Zhi-Ping Wang et al.   GENOME BIOLOGY

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

(2014) Hui-Li Xing et al.   BMC PLANT BIOLOGY

Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

(2014) Yanpeng Wang et al.   NATURE BIOTECHNOLOGY

Genome editing in rice and wheat using the CRISPR/Cas system

(2014) Qiwei Shan et al.   Nature Protocols

The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation

(2014) Hui Zhang et al.   PLANT BIOTECHNOLOGY JOURNAL

Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System

(2014) C. Brooks et al.   PLANT PHYSIOLOGY

Targeted Genome Editing of Sweet Orange Using Cas9/sgRNA

(2014) Hongge Jia et al.   PLoS One

Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis

(2014) Z. Feng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation

(2014) M. Jinek et al.   SCIENCE

Structural and functional partitioning of bread wheat chromosome 3B

(2014) F. Choulet et al.   SCIENCE

Draft genome of the wheat A-genome progenitor Triticum urartu

(2013) Hong-Qing Ling et al.   NATURE

Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9

(2013) Jian-Feng Li et al.   NATURE BIOTECHNOLOGY

Targeted genome modification of crop plants using a CRISPR-Cas system

(2013) Qiwei Shan et al.   NATURE BIOTECHNOLOGY

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE