期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 20, 期 17, 页码 -出版社
MDPI
DOI: 10.3390/ijms20174094
关键词
hindlimb unloading; chronic low-dose irradiation; brain; transcriptome
资金
- NASA [80NSSC17K0693, NNX13AL97G]
- NASA Space Biology Program
- NASA Space Life and Physical Sciences Research and Applications Division through the GeneLab Project
- BBSRC [BB/N015894/1]
- NIHR Nottingham Biomedical Research Centre
- National Institutes of Health (NIH) [S10OD019960]
- Ardmore Institute of Health (AIH) [2150141]
- MUSC Center for Genomics Medicine Bioinformatics
- Charles A. Sims' gift
- NASA [NNX13AL97G, 468860] Funding Source: Federal RePORTER
- BBSRC [BB/N015894/1] Funding Source: UKRI
Spaceflight poses many challenges for humans. Ground-based analogs typically focus on single parameters of spaceflight and their associated acute effects. This study assesses the long-term transcriptional effects following single and combination spaceflight analog conditions using the mouse model: simulated microgravity via hindlimb unloading (HLU) and/or low-dose gamma -ray irradiation (LDR) for 21 days, followed by 4 months of readaptation. Changes in gene expression and epigenetic modifications in brain samples during readaptation were analyzed by whole transcriptome shotgun sequencing (RNA-seq) and reduced representation bisulfite sequencing (RRBS). The results showed minimal gene expression and cytosine methylation alterations at 4 months readaptation within single treatment conditions of HLU or LDR. In contrast, following combined HLU+LDR, gene expression and promoter methylation analyses showed multiple altered pathways involved in neurogenesis and neuroplasticity, the regulation of neuropeptides, and cellular signaling. In brief, neurological readaptation following combined chronic LDR and HLU is a dynamic process that involves pathways that regulate neuronal function and structure and may lead to late onset neurological sequelae.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据