期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 141, 期 -, 页码 1220-1227出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.09.086
关键词
Platycodon grandiflorus polysaccharides; 3D4/21 cells; Mitochondrial apoptosis pathway
资金
- Shandong Natural Science Foundation of China [ZR2017MC026]
- China Postdoctoral Science Foundation [2017T100505, 2016M592232]
- Special Funding of Postdoctoral Innovation Project in Shandong Province [201603051]
- Natural Science Foundation of China [31402325]
- Funds of Shandong Double Tops Program
This study aimed to investigate the potential protective effects of Platycodon grandiflorus polysaccharide (PGPS) on carbonyl cyanide 3-chlorophenylhydrazone (CCCP)-induced mitochondrial apoptosis in 3D4/21 cells. Apoptosis-related indicators such as cell viability, apoptosis rate, mitochondrial membrane potential (MMP), and apoptosis-related protein were examined. Results indicated that PGPS(t) can inhibit CCCP-induced cell damage, with cell-survival rate reaching 81% and apoptotic rate decreasing to 23%. Nuclear deformation was also significantly reduced in the PGPS(t) group, and changes in MMP were inhibited by PGPS(t). Further analyses showed that the protein expression of Caspase-9 and Bcl-2 increased and the expression of cleaved Caspase-3 decreased, indicating that PGPS(t) significantly inhibited the CCCP-induced change in apoptotic protein expression. All these results suggested that PGPS(t) can antagonize 3D4/21 cell apoptosis by restoring MMP, protecting the integrity of nuclear morphology, and increasing Bcl-2 expression. (C) 2019 Elsevier B.V. All rights reserved.
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