期刊
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 112, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2019.103180
关键词
Plutella xylostella; Doublesex; Sex determination; CRISPR/Cas9; Sterility
资金
- National Science Foundation of China [31420103918]
- Strategic Priority Research Program of Chinese Academy of Sciences [XDB11010600]
- National Basic Research Program of China [2015CB755703]
Doublesex (dsx): the downstream gene in the insect sex determination pathway, plays a critical role in sexual differentiation and development. The functions of dsx have been characterized in several model insect species. However, the molecular mechanism and functions of sex determination of dsx in Plutella xylostella, an agricultural pest, are still unknown. In present study, we identified a male-specific and three female-specific Pxdsx transcripts in P. xylostella. Phylogenetic analyses and multiple sequence alignment revealed that Pxdsx is highly conserved in lepidopterans. The CRISPR/Cas9 technology was used to induce mutations in the male-specific isoform, the female-specific isoform, and common regions of Pxdsx. Disruptions of Pxdsx sex-specific isoforms caused sex-specific defects in external genitals and partial sexual reversal. In addition, we found that female specific transcripts were detected in Pxdsx(M) male mutants and male-specific transcripts were detected in Pxdsx(F) female mutants. Mutations also caused changes in expression of several sex-biased genes and induced sex-specific sterility. This study demonstrates that Pxdsx plays a key role in sex determination of P. xylostella and suggests novel genetic control approaches for the management of P. xylostella.
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