Article
Environmental Sciences
Dandan Tian, Wei Shi, Yihan Yu, Weishang Zhou, Yu Tang, Weixia Zhang, Lin Huang, Yu Han, Guangxu Liu
Summary: This study assessed the behavioral effects of enrofloxacin, a typical fluoroquinolone antibiotic, on zebrafish, and investigated its impact on the microbiota-gut-brain axis. Zebrafish exposed to 60μg/L enrofloxacin displayed abnormal anxiety-like behaviors and exhibited changes in the gut microbiota composition. Furthermore, exposure to enrofloxacin also led to alterations in the levels of certain biomarkers related to stress and anxiety in the brain. The disruption of the microbiota-gut-brain axis may contribute to enrofloxacin-induced anxiety-like behaviors in zebrafish.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Immunology
Heidi Mathiessen, Sebastian Kjeldgaard-Nintemann, Carlota Marola Fernandez Gonzalez, Cyril Henard, Julie Algreen Reimer, Sara Vebaek Gelskov, Moonika Haahr Marana, Louise von Gersdorff Jorgensen
Summary: This study investigated the early immune responses in zebrafish larvae infected with the parasite Ichthyophthirius multifiliis. The results showed that the larvae exhibited mild inflammation upon infection, and the 12-day-old larvae were less susceptible compared to the 5-day-old larvae. Neutrophils and macrophages were attracted to the parasites and exhibited neutrophil extracellular traps (NETs) during the fight against the parasite. The parasite employed rotation as an immune evasive strategy, but occasionally, neutrophils and macrophages were able to kill the parasite. In conclusion, the zebrafish larvae responded with localized high activity from neutrophils and macrophages, but only displayed mild inflammation systemically.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Medicine, Research & Experimental
Maria do Socorro Costa, Ana Raquel Pereira da Silva, Nara Juliana Santos Araujo, Jose Maria Barbosa Filho, Josean Fechine Tavares, Thiago Sampaio de Freitas, Francisco Nascimento Pereira, Erlanio Oliveira de Sousa, Francisco Paulo Araujo Maia, John Eversong Lucena de Vasconcelos, Jacqueline Cosmo Andrade Pinheiro, Henrique Douglas Melo Coutinho
Summary: This study reported the antimicrobial activity of diosgenin against various bacteria and evaluated its efflux pump inhibitory activity. The results showed that diosgenin enhanced the antimicrobial activity of certain antibiotics and did not interfere with the efflux pump mechanism.
Article
Cell Biology
Wanbin Hu, Leonie van Steijn, Chen Li, Fons J. Verbeek, Lu Cao, Roeland M. H. Merks, Herman P. Spaink
Summary: In this study using zebrafish larval tail-wounding model, it was found that TLR2 and MyD88 play a role in regulating neutrophil and macrophage cell migration behavior, influencing the direction of neutrophil migration away from the wound and the speed of macrophage migration towards the wound edge. This indicates the involvement of TLR2 and MyD88 in responses to tail wounding by modulating leukocyte migration behavior and speed in vivo.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Irene Pardo-Sanchez, Diana Garcia-Moreno, Victoriano Mulero
Summary: Melanoma is a deadly form of skin cancer and understanding its development is crucial for early detection and treatment.
Article
Immunology
Stefan Muenster, Valentina Zschernack, Birte Dierig, Stilla Frede, Georg Baumgarten, Mark Coburn, Christian Putensen, Christina Katharina Weisheit
Summary: The antibiotics vancomycin and daptomycin have immunomodulatory effects on cells involved in innate immunity in a murine model of LPS-induced sepsis. Further studies are needed to evaluate whether these antibiotics exhibit synergistic effects in the treatment of septic patients, beyond their bactericidal properties.
INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY
(2021)
Article
Engineering, Biomedical
Lais Morandini, Derek Avery, Benjamin Angeles, Paul Winston, Rebecca K. Martin, Henry J. Donahue, Rene Olivares-Navarrete
Summary: Neutrophils play a crucial role in the immune response to biomaterial implantation by releasing cytokines, chemokines, and DNA-based networks called NETs. In this study, we found that inhibiting NET formation suppresses pro-inflammatory macrophage activation and accelerates the inflammatory phase of healing, leading to greater bone formation around the implanted biomaterial. These findings highlight the importance of the neutrophil response and innate immune cell regulation during the integration of biomaterials.
ACTA BIOMATERIALIA
(2023)
Article
Microbiology
Armin Rashidi, Maryam Ebadi, Tauseef Ur Rehman, Heba Elhusseini, Harika Nalluri, Thomas Kaiser, Shernan G. Holtan, Alexander Khoruts, Daniel J. Weisdorf, Christopher Staley
Summary: Our study found that despite heavy and sustained antibiotic pressure, microbial communities within the same patient remained more similar to each other than to those from other patients. We identified 6 taxa within the pre-treatment microbiota that predicted the extent of antibiotic-induced perturbations. Our results suggest that specific species in the pre-treatment microbiota play a key role in determining personalized microbiota responses to antibiotics in humans, which could lead to precision interventions to prevent antibiotic-induced dysbiosis and its adverse clinical consequences.
Article
Biotechnology & Applied Microbiology
David Mealiea, Emilie Boudreau, Naomi De Silva, Lili Okamoto, Tiffany Ho, Jason E. Fish, J. Andrea McCart
Summary: The zebrafish tumor xenograft model has been adapted for oncolytic virotherapy research. It allows for tumor-specific infection and measurable antitumor effects, with the use of confocal fluorescent imaging and the natural transparency of zebrafish embryos for real-time observation of important events during virotherapy.
CANCER GENE THERAPY
(2021)
Article
Microbiology
Madeleine Spatz, Gregory Da Costa, Rebecka Ventin-Holmberg, Julien Planchais, Chloe Michaudel, Yazhou Wang, Camille Danne, Alexia Lapiere, Marie-Laure Michel, Kaija-Leena Kolho, Philippe Langella, Harry Sokol, Mathias L. Richard
Summary: This study investigated the effects of antibiotic treatment on the fungal microbiota in the gut. It was found that amoxicillin-clavulanic acid treatment led to a decrease in the overall fungal population in mouse feces and a remodeling of the fungal community. Certain bacterial strains were shown to inhibit fungal growth. This study provides new insights into the interactions between fungi and bacteria in the gut and may offer new strategies for modulating gut microbiota equilibrium.
Article
Cell Biology
Abdulsalam I. Isiaku, Zuobing Zhang, Vahid Pazhakh, Harriet R. Manley, Ella R. Thompson, Lucy C. Fox, Satwica Yerneni, Piers Blombery, Graham J. Lieschke
Summary: The study introduced transgenic lines allowing for the distinction of phagocyte function in zebrafish, demonstrating the individual contributions of neutrophil- and macrophage-expressed genes to various physiological processes and diseases. Efficient gene editing was achieved in both neutrophils and macrophages, providing improved tools for research in zebrafish models.
DISEASE MODELS & MECHANISMS
(2021)
Article
Medicine, Research & Experimental
Megha Srinivas, Preeti Sharma, Siddharth Jhunjhunwala
Summary: Research findings suggest that immune cells can phagocytose particles under constant fluidic flow. Different cell types show similar or better uptake rates under flow conditions. Even in the presence of crowded red blood cells, neutrophils and monocytes are able to uptake particles while flowing.
MOLECULAR PHARMACEUTICS
(2021)
Article
Environmental Sciences
Weishang Zhou, Wei Shi, Xueying Du, Yu Han, Yu Tang, Sanghyok Ri, Kwangjin Ju, Tongchol Kim, Lin Huang, Weixia Zhang, Yihan Yu, Dandan Tian, Yingying Yu, Liangbiao Chen, Zhichao Wu, Guangxu Liu
Summary: This study evaluated the toxicity of microplastics (MPs) and antibiotic residues related to the occurrence of nonalcoholic fatty liver disease (NAFLD) using a zebrafish model. The results showed that exposure to MPs and oxytetracycline (OTC) resulted in more severe NAFLD symptoms, including lipid accumulation, liver inflammation, and hepatic oxidative stress.
ENVIRONMENTAL HEALTH PERSPECTIVES
(2023)
Article
Gastroenterology & Hepatology
Anders Brunse, Simone Margaard Offersen, Josefine Juliane Mosegaard, Ling Deng, Peter Damborg, Dennis Sandris Nielsen, Per Torp Sangild, Thomas Thymann, Duc Ninh Nguyen
Summary: In this study using preterm piglets, it was found that there is an antagonistic relationship between enteral antibiotics and fecal microbiota transplantation in preventing necrotizing enterocolitis, with minor effects on gut microbiota composition and immune responses.
Article
Chemistry, Multidisciplinary
Xuemei Liu, Geng Dou, Zihan Li, Xiangdong Wang, Ronghua Jin, Yao Liu, Huijuan Kuang, Xiaoyao Huang, Xiaoxue Yang, Xiaoshan Yang, Siying Liu, Meiling Wu, Hao Guo, Feng Ding, Haokun Xu, Shiyu Liu, Yan Jin, Kun Xuan
Summary: This study successfully promotes tissue regeneration in critical-sized bone defects and cutaneous wounds by manipulating the inflammatory responses, providing a new strategy for refractory wound therapy.
