期刊
FEBS LETTERS
卷 593, 期 23, 页码 3370-3380出版社
WILEY
DOI: 10.1002/1873-3468.13612
关键词
direct reprogramming; electrophysiology; human adult neurons; induced neurons; miR124; miR9
资金
- H2020 European Research Council [602278, 30971, FP/2007-2013] Funding Source: Medline
- MarieCurie Horizon 2020 Funding Source: Medline
- Swedish Research Council [70862601, 521-2012-5624] Funding Source: Medline
- Multidisciplinary research in Parkinson's disease (MultiPark) Funding Source: Medline
- Fonds du Québec en Recherche, Santé (FRQS) Funding Source: Medline
- Swedish Parkinson Foundation Funding Source: Medline
Direct neuronal reprogramming can be achieved using different approaches: by expressing neuronal transcription factors or microRNAs; and by knocking down neuronal repressive elements. However, there still exists a high variability in terms of the quality and maturity of the induced neurons obtained, depending on the reprogramming strategy employed. Here, we evaluate different long-term culture conditions and study the effect of expressing the neuronal-specific microRNAs, miR124 and miR9/9*, while reprogramming with forced expression of the transcription factorsAscl1,Brn2, and knockdown of the neuronal repressor REST. We show that the addition of microRNAs supports neuronal maturation in terms of gene and protein expression, as well as in terms of electrophysiological properties.
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