期刊
EUROPEAN JOURNAL OF NEUROSCIENCE
卷 50, 期 11, 页码 3772-3785出版社
WILEY
DOI: 10.1111/ejn.14553
关键词
alpha receptor; hippocampus; noradrenaline; norepinephrine; prefrontal cortex
资金
- Deutsche Forschungsgemeinschaft [CRC 1080]
- Gutenberg Forschungskolleg
The locus coeruleus (LC) contains the majority of central noradrenergic neurons sending wide projections throughout the entire CNS. The LC is considered to be essential for multiple key brain functions including arousal, attention and adaptive stress responses as well as higher cognitive functions and memory. Electrophysiological studies of LC neurons have identified several characteristic functional features such as low-frequency pacemaker activity with broad action potentials, transient high-frequency burst discharges in response to salient stimuli and an apparently homogeneous inhibition of firing by activation of somatodendritic alpha 2 autoreceptors (alpha 2AR). While stress-mediated plasticity of the alpha 2AR response has been described, it is currently unclear whether different LC neurons projecting to distinct axonal targets display differences in alpha 2AR function. Using fluorescent beads-mediated retrograde tracing in adult C57Bl6/N mice, we compared the anatomical distributions and functional in vitro properties of identified LC neurons projecting either to medial prefrontal cortex, hippocampus or cerebellum. The functional in vitro analysis of LC neurons confirmed their mostly uniform functional properties regarding action potential generation and pacemaker firing. However, we identified significant differences in tonic and evoked alpha 2AR-mediated responses. While hippocampal-projecting LC neurons were partially inhibited by endogenous levels of norepinephrine and almost completely silenced by application of saturating concentrations of the alpha 2 agonist clonidine, prefrontal-projecting LC neurons were not affected by endogenous levels of norepinephrine and only partially inhibited by saturating concentrations of clonidine. Thus, we identified a limited alpha 2AR control of electrical activity for prefrontal-projecting LC neurons indicative of functional heterogeneity in the LC-noradrenergic system.
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