Article
Biotechnology & Applied Microbiology
Jinduo Wang, Shuo Wang, Siyu Zhao, Pengjie Sun, Zhen Zhang, Qingyang Xu
Summary: This study designed and developed an oxygen-enhanced bioreactor to improve oxygen supply during lysine fermentation. The bioreactor optimized the aeration mix and used oxygen vectors to enhance the ability of cells to take up oxygen. This solution effectively increased the yield and conversion rate of lysine.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Jie Liu, Jian-Zhong Xu, Zhi-Ming Rao, Wei-Guo Zhang
Summary: This article briefly introduces the wide application of L-lysine in food, pharmaceuticals, and feed additives, as well as its production and regulation mechanism in Corynebacterium glutamicum. It also details the strategies for constructing high-producing strains, scaling up culture, and separating and purifying the product, as well as new breeding and fermentation methods.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Computer Science, Interdisciplinary Applications
Christian Spreafico, Matteo Spreafico
Summary: This paper introduces a text mining method to automatically retrieve knowledge from patents about waste recycling and reuse. By using specific dependency patterns and a revised TRIZ ontology, the classification accuracy and retrieval efficiency are improved.
COMPUTERS IN INDUSTRY
(2021)
Article
Green & Sustainable Science & Technology
Philipp Metzger, Sandro Mendonca, Jose A. Silva, Bruno Damasio
Summary: This study examines the long-term progress of a knowledge base in secondary battery innovations, which plays a crucial role in the transition to a transformative, closed-loop, Circular Economy. The analysis of over 90,000 international patent families reveals global acceleration of innovation, mainly originating in Asia, with patterns indicating less toxicity and more diversity in technological trajectories that promote circularity. Emerging technological trajectories, such as solid-state, lithium-sulfur, redox-flow, and sodium-ion batteries, show different potential to advance the circularity pathway and allow for the identification of country clusters. Using an interesting methodology, the study evaluates the circular characteristics of batteries.
Article
Pharmacology & Pharmacy
Juliana Rincon-Lopez, Yara C. Almanza-Arjona, Alejandro P. Riascos, Yareli Rojas-Aguirre
Summary: By analyzing a dataset of CD pharmaceutical patents, a data-driven approach was used to unveil pharmaceutical technologies of cyclodextrins (CDs). Complex networks formed by CD patents demonstrated the supremacy of CDs for solubility enhancement and triggered cutting-edge applications. CDs play a crucial role in formulating aqueous solutions, tablets, and powders, with text-mining showing increasing trends in these dosage forms.
Review
Pharmacology & Pharmacy
Juliana Rincon-Lopez, Yara C. Almanza-Arjona, Alejandro P. Riascos, Yareli Rojas-Aguirre
Summary: This research reveals the evolution of cyclodextrin-based pharmaceutical technologies with a focus on solubility, stability, and taste-masking enhancement. The majority of these technologies are directed toward parenteral aqueous solutions, while oral and ocular formulations are rapidly growing. Although formulations for nasal and pulmonary routes are emerging, other routes such as topical, transdermal, vaginal, and rectal have not been widely explored but may hold great potential. The progress in materials sciences, supramolecular chemistry, and nanotechnology is expected to further influence the development of these pharmaceutical technologies.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2021)
Review
Multidisciplinary Sciences
Gergely Mark Csanyi, Daniel Nagy, Renato Vagi, Janos Pal Vadasz, Tamas Orosz
Summary: Data sharing in judicial systems can enhance transparency, but the sensitive information in legal documents must be anonymized to prevent privacy breaches. Named Entity Recognition methods, machine learning, and anonymization models like differential privacy are crucial for reducing re-identification risk.Preserving the utility of the text while anonymizing legal documents is essential.
Article
Business
Chen Tian, Junyan Zhang, Dayong Liu
Summary: This article comprehensively investigates the differences between standard-essential patents (SEPs) and nonstandard-essential patents (N-SEPs) in terms of knowledge sources, novelty, and generality. It found that SEPs have lower technological diversity and scientific linkage, but higher standard linkage compared to N-SEPs. SEPs are more generic and less novel than N-SEPs. Different knowledge sources have distinct effects on the novelty and generality of SEPs and N-SEPs.
IEEE TRANSACTIONS ON ENGINEERING MANAGEMENT
(2023)
Article
Biotechnology & Applied Microbiology
Abhijith Suvachan, Roshan Lal, K. Madhavan Nampoothiri, Raveendran Sindhu, Thallada Bhaskar, Parameswaran Binod, Ashok Pandey, Rakesh Yasarla
Summary: The paper and pulp industry generates a significant amount of waste material and waste water. One recent trend is the conversion of industrial waste into renewable products through green processes for the production of renewable fuels and other bioproducts. This study focuses on utilizing paper industry rejects for the production of amino acids, and explores the effects of acid pretreatment and enzymatic hydrolysis on cellulose fiber structure.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Article
Biotechnology & Applied Microbiology
Gang Meng, Wei Zhao, Qingguo Liu, Chunguang Zhao, Yanan Zou, Nan Zhao, Dong Liu, Hanjie Ying, Wenjun Sun, Yong Chen
Summary: This study describes a strategy for designing bioreactors for immobilized fermentation, which significantly improves the conversion rate and production through the construction of steel structures and the adjustment of ventilation system.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jiaping Wang, Cong Gao, Xiulai Chen, Liming Liu
Summary: A five-enzyme cascade pathway was designed to efficiently produce glutarate from l-lysine, with ribosome binding site regulation used to adjust the enzyme molar ratio and scanning techniques employed to enhance reaction efficiency. Through these strategies, a novel enzymatic process at industrial scale to produce glutarate from cheaper amino acids was successfully demonstrated.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Hai-De Wang, Jian-Zhong Xu, Wei-Guo Zhang
Summary: In this study, a high L-arginine (L-Arg) producing E. coli strain was obtained through metabolic engineering. The genes speC and speF were found to play important roles in L-Arg accumulation. Feedback inhibition of the L-Arg pathway in E. coli was successfully overcome by combining gene mutations/deletions. Additionally, the insertion of a gene cluster from Corynebacterium glutamicum enhanced the metabolic flux of the L-Arg pathway. The final strain achieved the highest reported level of L-Arg production by E. coli.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Agricultural Engineering
Qi Sheng, Xiaoyu Wu, Yan Jiang, Zhimin Li, Fei Wang, Bin Zhang
Summary: This study demonstrated the biobased production of L-ornithine from mannitol using engineered Corynebacterium glutamicum, providing a reference for the production of additional chemicals from mannitol.
BIORESOURCE TECHNOLOGY
(2021)
Article
Food Science & Technology
Si-Hui Liu, Juan Li, Jian-An Huang, Zhong-Hua Liu, Li-Gui Xiong
Summary: L-theanine, the most abundant non-proteinogenic amino acid in tea plants, is now extensively produced through microbial engineering processes as a sustainable manufacturing approach for its biosynthesis. Recent studies highlight the latest advances and key process parameters in L-theanine production using microbial platforms, showcasing the potential for successful industrial applications.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2021)
Article
Agricultural Engineering
Jie Liu, Ying Ou, Jian-Zhong Xu, Zhi-Ming Rao, Wei-Guo Zhang
Summary: This study describes the systems metabolic engineering of Corynebacterium glutamicum to enhance L-lysine production. Key chromosomal mutations related to L-lysine synthesis were identified through whole-genome sequencing. Carbon flux was redirected to the L-lysine synthesis pathway, increasing energy availability and product transport systems. A promoter library sensitive to intracellular L-lysine concentration was constructed and used for dynamic regulation of the NADPH pool. In fed-batch fermentation, the engineered strain achieved a L-lysine titer of 223.4 ± 6.5 g/L. This study is the first to improve L-lysine production by enhancing ATP supply and NADPH self-regulation, improving intracellular conditions.
BIORESOURCE TECHNOLOGY
(2023)
Review
Agricultural Engineering
Carlos Ricardo Soccol, Brigitte Sthepani Orozco Colonia, Gilberto Vinicius de Melo Pereira, Luis Daniel Goyzueta Mamani, Susan Grace Karp, Vanete Thomaz Soccol, Rafaela de Oliveira Penha, Carlos Jose Dalmas Neto, Julio Cesar de Carvalho
Summary: This review discusses recent advancements in lipid producing microorganism bioprospecting, highlighting the transition from traditional cultivation techniques to state-of-the-art metagenomics approaches. The review also focuses on the biotechnological potential of three promising microorganisms for the production of important compounds. These microbial lipids offer alternative solutions for the development of food, feed, and biofuels.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Natalia Notto Serena, Raphael Aparecido Boschero, Manuel Hospinal-Santiani, Violetta Dias Pacce, Jean Michel Dela Vedova Costa, Francisco Deraldi De Magalhaes, Carlos Wiedmar, Silvana Maria Alban, Carlos Ricardo Soccol, Vanete Thomaz-Soccol
Summary: This study identified new peptide mimotopes of Mycobacterium tuberculosis and applied them in the serodiagnosis of tuberculosis through immunoassays. The selected peptide demonstrated 100% sensitivity and specificity, providing a potential tool for tuberculosis screening.
JOURNAL OF IMMUNOLOGICAL METHODS
(2022)
Article
Agronomy
Susan G. Karp, Walter J. M. Burgos, Luciana P. S. Vandenberghe, Kim Diestra, Luis A. Z. Torres, Adenise L. Woiciechowski, Luiz A. J. Letti, Gilberto V. M. Pereira, Vanete Thomaz-Soccol, Cristine Rodrigues, Julio C. de Carvalho, Carlos R. Soccol
Summary: Sugarcane is a promising biomass resource in the developing bioeconomy, with Brazil being the leading producer. In addition to being a source of sugar, ethanol, and electricity, sugarcane has the potential for other bio-based products. The valorization of sugarcane residues in existing biorefineries also has enormous potential.
Article
Green & Sustainable Science & Technology
L. P. S. Vandenberghe, K. K. Valladares-Diestra, G. A. Bittencourt, L. A. Zevallos Torres, S. Vieira, S. G. Karp, E. B. Sydney, J. C. de Carvalho, V. Thomaz Soccol, C. R. Soccol
Summary: Sugarcane has become an important renewable raw material that can potentially reduce greenhouse gas emissions and decrease the dependence on fossil fuels. Brazilian government policies have created favorable conditions for the establishment of the first sugarcane biorefinery, which aims to produce biofuels, sugar, and bioelectricity in a sustainable and cost-effective manner. The development of 2G and 3G technologies further expands the potential of sugarcane for producing value-added biochemicals and reducing emissions through integrated systems with a non-waste vision and circular bioeconomy.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2022)
Article
Food Science & Technology
Alexander da Silva Vale, Gabriel Balla, Luiz Roberto Saldanha Rodrigues, Dao Pedro de Carvalho Neto, Carlos Ricardo Soccol, Gilberto Vinicius de Melo Pereira
Summary: This study investigated the microbial community structure and chemical changes in different layers of a static coffee beans fermentation tank over different time periods (24, 48, and 72 h). The results showed that extending the fermentation time resulted in increased production of key metabolites and diversification of sensory profiles of coffee beverages.
Article
Agricultural Engineering
Walter J. Martinez-Burgos, Luciana Porto de Souza Vandenberghe, Susan Grace Karp, Ariane Fatima Murawski de Mello, Vanete Thomaz Soccol, Carlos Ricardo Soccol
Summary: Soybean hulls, a lignocellulosic residue from soybean processing, can be valorized as a carbon source for biolipid production. This study optimized the process for producing lipids from soybean hulls using Lipomyces starkeyi LPB 53 strain. The maximum cell biomass concentration reached was 26.5 g/L with 42.5% lipids, and a significant portion of xylose was consumed. The obtained oil was mainly composed of oleic, palmitic, palmitoleic, linoleic, and stearic fatty acids.
BIORESOURCE TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Beatriz A. Braz, Manuel Hospinal-Santiani, Gustavo Martins, Jeferson L. Gogola, Marcia G. P. Valenga, Breno C. B. Beirao, Marcio F. Bergamini, Luiz H. Marcolino-Junior, Vanete Thomaz-Soccol, Carlos R. Soccol
Summary: Electrochemical immunosensors with synthetic peptides and nanomaterials such as gold nanoparticles have been used to detect SARS-CoV-2 Anti-S antibodies. A solid-binding peptide was used as the recognition site, capable of recognizing antibodies of the spike protein. The immunosensor showed good selectivity and sensitivity for antibody detection in human serum samples.
Review
Biotechnology & Applied Microbiology
Julio Cesar de Carvalho, Luciana Porto de Souza Vandenberghe, Eduardo Bittencourt Sydney, Susan Grace Karp, Antonio Irineudo Magalhaes Jr, Walter Jose Martinez-Burgos, Adriane Bianchi Pedroni Medeiros, Vanete Thomaz-Soccol, Sabrina Vieira, Luiz Alberto Junior Letti, Cristine Rodrigues, Adenise Lorenci Woiciechowski, Carlos Ricardo Soccol
Summary: Sugarcane ethanol production generates a large amount of vinasse, which can be used as a suitable substrate for anaerobic digestion to improve energy output. This review discusses the current technology for ethanol production from sugarcane and describes the state of the art in vinasse anaerobic digestion.
FERMENTATION-BASEL
(2023)
Review
Biotechnology & Applied Microbiology
Bianca de Oliveira Hosken, Gilberto Vinicius Melo Pereira, Thamylles Thuany Mayrink Lima, Joao Batista Ribeiro, Walter Coelho Pereira de Magalhaes Jr, Jose Guilherme Prado Martin
Summary: Artisanal cheeses in Brazil have a great diversity and cultural importance. Each producing region has a characteristic microbiota, mainly composed of lactic acid bacteria (LAB), which play important roles in technological, sensory, and safety characteristics. This review summarizes the biotechnological applications of LAB in different artisanal cheeses and highlights the challenges and opportunities in this field.
FERMENTATION-BASEL
(2023)
Review
Biotechnology & Applied Microbiology
Alexander da Silva Vale, Barbara Cogo Venturim, Andre Ricardo Ferreira da Silva Rocha, Jose Guilherme Prado Martin, Bruna Leal Maske, Gabriel Balla, Juliano De Dea Lindner, Carlos Ricardo Soccol, Gilberto Vinicius de Melo Pereira
Summary: In recent years, the development of non-dairy probiotic beverages has been driven by the increasing number of people with milk protein allergies, lactose intolerance, and vegetarian and vegan diets. These functional foods have health benefits and combine plant properties with probiotic effects. However, the limited availability of suitable microbial strains with probiotic characteristics that also meet sensory requirements poses a challenge in formulating these beverages. This review provides an overview of traditional non-dairy fermented beverages worldwide and highlights the biotechnological potential of these products as a source of probiotic strains. It also discusses recent developments in using lactic acid bacteria, Bifidobacterium, and yeast species to create new probiotic beverages from fruits and cereals. Additionally, food safety issues are addressed.
FERMENTATION-BASEL
(2023)
Review
Biotechnology & Applied Microbiology
Alexander da Silva Vale, Gilberto Vinicius de Melo Pereira, Ana Caroline de Oliveira, Dao Pedro de Carvalho Neto, Leonardo Wedderhoff Herrmann, Susan Grace Karp, Vanete Thomaz Soccol, Carlos Ricardo Soccol
Summary: The skin microbiome, consisting of bacteria, fungi, and viruses, plays a critical role in preventing the overgrowth of harmful microorganisms. Probiotic-derived metabolites, known as postbiotics, have emerged as promising bioactive compounds for skin health. Postbiotics, produced by lactic acid bacteria and yeast, possess antimicrobial, immunomodulatory, antioxidant, and anti-inflammatory activities. They also offer advantages in terms of stability and safety compared to probiotics. This article reviews the large-scale production of postbiotics and their applications in cosmetic formulations, as well as discussing their efficacy in treating various skin conditions. Technological advancements in the field of postbiotics are also highlighted through a survey of patents.
FERMENTATION-BASEL
(2023)
Article
Chemistry, Analytical
Beatriz A. Braz, Manuel Hospinal-Santiani, Gustavo Martins, Breno C. B. Beirao, Marcio F. Bergamini, Luiz H. Marcolino-Junior, Carlos R. Soccol, Vanete Thomaz-Soccol
Summary: Neglected tropical diseases, which are endemic in low-income populations, lack research investment and have unacceptable indicators. Electrochemical immunosensors show promise for diagnosis in isolated regions. In this study, different peptides were investigated for detecting Leishmania antibodies with outstanding performance achieved using a graphene affinity peptide as a recognition site.
Review
Food Science & Technology
Kim Kley Valladares-Diestra, Luciana Porto de Souza Vandenberghe, Sabrina Vieira, Luis Daniel Goyzueta-Mamani, Patricia Beatriz Gruening de Mattos, Maria Clara Manzoki, Vanete Thomaz Soccol, Carlos Ricardo Soccol
Summary: Concerns about a good-quality diet have risen in recent years, leading to an increased interest in food supplements like prebiotics that offer great nutritional and health benefits. Xylooligosaccharides (XOs) show high potential among various prebiotics for preventing systemic disorders. While XOs can be found in natural sources, their production is limited. Lignocellulosic biomasses have been identified as a promising source for XOs production, using agro-industrial by-products. However, physicochemical pretreatments are required to obtain XOs, and different methods are discussed to increase their production and limit the production of toxic compounds.
Article
Parasitology
Felipe Dutra Rego, Eduardo Sergio da Silva, Valeriana Valadares Lopes, Rafael Goncalves Teixeira-Neto, Vinicius Silva Belo, Antonio Augusto Fonseca Jr, Diego Andrade Pereira, Heber Paulino Pena, Marcia Dalastra Laurenti, Gabriela Araujo, Vania Lucia Ribeiro da Matta, Islam Hussein Chouman, Thaina Bergantin Burrin, Carmen M. Sandoval, Stella Maria Barrouin-Melo, Flaviane Alves de Pinho, Helida Monteiro de Andrade, Ramon Vieira Nunes, Celia Maria Ferreira Gontijo, Vanete Thomaz Soccol, Donnamae Klocek, Danyil Grybchuk, Diego Henrique Macedo, Rubens Lima do Monte-Neto, Vyacheslav Yurchenko, Rodrigo Pedro Soares
Summary: This study is the first to detect the presence of LRV2 in the New World, which is significant for understanding the transmission and impact of this virus.
MEMORIAS DO INSTITUTO OSWALDO CRUZ
(2023)