Article
Biotechnology & Applied Microbiology
Xin Luo, Karl R. Matthews
Summary: The emergence of mobile colistin resistant gene (mcr-1) in Enterobacteriaceae has become a global public health concern. This study investigated the transfer frequency of the mcr-1 gene among Escherichia coli in liquid media and during growth of mung bean sprouts. The results showed that the transfer of mcr-1 gene was more frequent at higher temperatures and during mung bean sprout growth. This study suggests that the production of mung bean sprouts may contribute to the spread of antibiotic resistant bacteria.
Article
Food Science & Technology
Shuhong Zhang, Yuanbin Huang, Moutong Chen, Guangzhu Yang, Jumei Zhang, Qingping Wu, Juan Wang, Yu Ding, Qinghua Ye, Tao Lei, Yue Su, Rui Pang, Runshi Yang, Youxiong Zhang
Summary: This study systematically characterized E. coli O157:non-H7 strains isolated from retail food in Chinese markets. The strains displayed diverse H serotypes, virulence genes, and antibiotic resistance. Some strains also harbored plasmid-mediated colistin-resistant genes, which is a significant concern.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Microbiology
Kei Amemiya, David A. Rozak, Jennifer L. Dankmeyer, William R. Dorman, Charles Marchand, David P. Fetterer, Patricia L. Worsham, Brett K. Purcell
Summary: In 2011, Germany experienced a major outbreak of bloody diarrhea caused by an atypical E. coli O104:H4 strain. This strain showed phenotypic differences compared to the O157:H7 strain, such as higher biofilm production and the ability to grow in a novel HEK293 cell culture medium. The study uncovered previously unknown phenotypic properties of E. coli O104:H4 to further differentiate it from E. coli O157:H7.
Article
Environmental Sciences
Katharina Detert, Jonathan Waehrer, Kay Nieselt, Herbert Schmidt
Summary: In this study, the metabolic genes and networks that affect the persistence of pathogenic Escherichia coli O104:H4 strain C227/11fcu in agricultural soil microenvironments at low temperature were explored. RNA sequencing analysis revealed differential expression of over 1500 genes at 1 and 4 weeks compared to the initial time point, including stress response genes and genes involved in primary metabolism. Genes and gene sets related to the uptake of carbohydrates and amino acids were significantly upregulated in low nutrient environments. These transcriptome analysis results suggest a complex interplay of metabolic networks in the persistence of C227/11fcu in soils.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2023)
Article
Food Science & Technology
Yuan Fang, Luisa Linda Bruckner, Lynn M. McMullen, Michael G. Ganzle
Summary: The study shows that the bacteriophage 011-3088 from Escherichia coli O104:H4 can transduce other strains of E. coli, including pathogenic strains, during sprouting. The expression level of the bacteriophage differs among lysogens upon induction, which may result in infections.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Sarah H. Klass, Laura E. Sofen, Zachary F. Hallberg, Tahoe A. Fiala, Alexandra Ramsey, Nicholas S. Dolan, Matthew B. Francis, Ariel L. Furst
Summary: Pathogenic E. coli strains are a significant threat to public health, causing both foodborne illnesses and urinary tract infections. By using a rapid bioconjugation reaction and electrochemical impedance spectroscopy, we can selectively capture and accurately quantify these pathogenic microbes on a disposable gold electrode.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Smriti Shringi, Haiqing Sheng, Andrew A. Potter, Scott A. Minnich, Carolyn J. Hovde, Thomas E. Besser
Summary: Oral immunization with live stx-negative LEE+ E. coli O157:H7 in cattle reduces the colonization of wild-type E. coli O157:H7 strains at the rectoanal junction and shortens the shedding duration.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Ting Zheng, Xianming Li, Ya-Ni Xie, Bin Yang, Peng Wu
Summary: This study proposes a dual-gene RPA-LFA method to selectively detect E. coli O157:H7 by recognizing target amplicons and eliminating false positives. The assay shows high sensitivity and specificity for the detection of this pathogen.
ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Man-Seok Choi, Eun Bi Jeon, Ji Yoon Kim, Eun Ha Choi, Jun Sup Lim, Jinsung Choi, Shin Young Park
Summary: The efficacy of dielectric barrier discharge (DBD) plasma treatment for reducing non-pathogenic Escherichia coli and E. coli O157:H7 in oysters was studied. Exposure to DBD plasma for at least 60 minutes resulted in significant reductions in both types of bacteria, with minimal impact on the glycogen content and texture of the oysters. This non-thermal intervention could be a promising method for improving the safety of fresh oysters.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Immunology
Philipp Schiller, Michael Knoedler, Petya Berger, Lilo Greune, Angelika Fruth, Alexander Mellmann, Petra Dersch, Michael Berger, Ulrich Dobrindt
Summary: The study found differences in adherence properties and pathogenicity between the EHEC outbreak strain O104:H4 and its close relative due to expression of different types of aggregative adherence fimbriae (AAF). Various types of AAF affect bacterial autoaggregation, biofilm formation, and host cell adherence.
Article
Biochemistry & Molecular Biology
Meihong Cai, Gang Zhang, Changzhu Li, Xiaochen Chen, Haiying Cui, Lin Lin
Summary: This study introduced pomegranate peel polyphenols embedded in chitosan to prepare antibacterial nanofibers, showing effective inhibition of bacterial growth on food surfaces (e.g. E. coli) without affecting food quality. By adding different concentrations of polyphenols and polysaccharides, the properties of nanofibers can be controlled to enhance encapsulation rate and tensile strength.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Veterinary Sciences
Aklilu Feleke Haile, Silvia Alonso, Nega Berhe, Tizeta Bekele Atoma, Prosper N. Boyaka, Delia Grace
Summary: This study estimated the prevalence and antimicrobial resistance of Escherichia coli O157:H7 in raw beef sold in butcher shops in Addis Ababa, Ethiopia. Out of the 384 samples examined, 3.64% carried E. coli O157:H7, with 57.14% of the isolates showing resistance to three or more antimicrobial agents. Thus, stricter monitoring of antimicrobial use and further studies on contamination sources and risk mitigation strategies are needed.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biophysics
Bin Chen, Qin Tao, Songtao OuYang, Minhui Wang, Yuanjian Liu, Xiaohui Xiong, Songqin Liu
Summary: This study demonstrates that E. coli O157:H7 can electrochemically catalyze oxygen reduction at the cathode of bipolar electrode (BPE), resulting in electrochemiluminescence (ECL) reporting intensity change at the anode. Most food matrix does not exhibit catalytic activity for electrochemical reduction of O-2, and their interference with the ECL reaction at the anode is negligible. A low detection limit of 10 CFU mL(-1) for E. coli O157:H7 can be achieved within 1 hour using this system, making it an excellent tool for rapid detection of electroactive bacteria in food.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Food Science & Technology
Kai-Sheng Shen, Mei Shu, Meng-Xuan Tang, Wen-Yu Yang, Shu-Chao Wang, Chan Zhong, Guo-Ping Wu
Summary: This study cloned, expressed, and characterized an endolysin named LysJN01 from bacteriophage JN01. The recombinant LysJN01 showed strong lytic activity against E. coli O157:H7 and exhibited broad-spectrum antimicrobial activity against Gram-negative pathogens. Additionally, it significantly reduced the count of E. coli O157:H7 on contaminated lettuce.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Immunology
Hossein Samiei, Shahram Nazarian, Abass Hajizade, Emad Kordbacheh
Summary: In this study, a chimeric protein comprising two antigens of Escherichia coli O157:H7 was designed and evaluated as a vaccine candidate using in silico techniques. The results showed that the proposed antigen has high antigenicity and conformation suitable for being a potent vaccine candidate. Immunization evaluation demonstrated that the protein is able to stimulate the immune responses of mice and provide protective immunity against E. coli O157:H7.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Immunology
Philipp Schiller, Michael Knoedler, Petya Berger, Lilo Greune, Angelika Fruth, Alexander Mellmann, Petra Dersch, Michael Berger, Ulrich Dobrindt
Summary: The study found differences in adherence properties and pathogenicity between the EHEC outbreak strain O104:H4 and its close relative due to expression of different types of aggregative adherence fimbriae (AAF). Various types of AAF affect bacterial autoaggregation, biofilm formation, and host cell adherence.
Article
Chemistry, Multidisciplinary
Andrea Sowa, Alexander Hoeing, Ulrich Dobrindt, Shirley K. Knauer, Anzhela Galstyan, Jens Voskuhl
Summary: This study describes the synthesis, characterization, and application of water-soluble zinc(II) phthalocyanines decorated with umbelliferone moieties for photodynamic therapy. These compounds exhibited excellent phototoxicity and antimicrobial activity in cells and bacteria, making them promising photosensitizers for future broad applications.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Neurosciences
Yvonne Huisman, Katharina Uphoff, Michael Berger, Ulrich Dobrindt, Mario Schelhaas, Thomas Zobel, Jeroen Bussmann, Andreas van Impel, Stefan Schulte-Merker
Summary: Brain lymphatic endothelial cells (BLECs) in zebrafish have been found to efficiently uptake extracellular cargo molecules compared to microglia. While BLECs are effective in uptaking proteins, polysaccharides, and virus particles, microglia are better at ingesting larger particles like bacteria, indicating a clear distribution of tasks between the two cell types. Additionally, BLECs show similar substrate acceptance as scavenger endothelial cells (SECs) of the cardinal vein, and cooperate with microglia to remove particle waste from the brain.
Article
Infectious Diseases
Ulrich Dobrindt, Haleluya T. Wami, Torsten Schmidt-Wieland, Daniela Bertsch, Klaus Oberdorfer, Herbert Hof
Summary: Nitroxoline remains effective against some multidrug resistant uropathogenic bacteria, particularly E. coli, suggesting it could be a potential alternative to cotrimoxazole for treating uncomplicated urinary tract infections.
Article
Multidisciplinary Sciences
James M. Gahan, Ian U. Kouzel, Kamilla Ormevik Jansen, Pawel Burkhardt, Fabian Rentzsch
Summary: This study reveals the role of chromatin modifier Lsd1 in developmental regulation in cnidarians. The findings suggest that the integration of chromatin modifying proteins into developmental regulation predates the divergence of cnidarians and bilaterians, and is an ancient feature of animal development.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Marla Keizers, Ulrich Dobrindt, Michael Berger
Summary: Bacteria use interspecies signaling molecules to control gene expression for the benefit of the bacterial community. The analysis and quantification of the signaling molecule AI-2 are important for understanding changes in bacterial behavior. Current bioassays for AI-2 are complex and sensitive, so researchers have developed a sensitive and reliable Escherichia coli biosensor-based assay for AI-2 quantification.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biology
Krishnendu Mukherjee, Ulrich Dobrindt
Summary: This article reviews the contributions of chromatin remodeling to insect immunity and adaptation to pathogens, focusing on the roles of DNA methylation, histone acetylation, and microRNAs in the evolution of insect resistance.
CURRENT OPINION IN INSECT SCIENCE
(2022)
Article
Evolutionary Biology
Nicolas C. H. Schroeder, Ana Korsa, Haleluya Wami, Olena Mantel, Ulrich Dobrindt, Joachim Kurtz
Summary: This study investigated the persistence and evolution of the probiotic Escherichia coli strain Nissle 1917 in an insect host and flour environment through experimental evolution. The findings suggest that EcN remains stable with weak phenotypic changes during serial passage in the insect host, indicating a certain degree of phenotypic plasticity.
EVOLUTION MEDICINE AND PUBLIC HEALTH
(2022)
Article
Toxicology
Robert Landsiedel, Daniela Hahn, Rainer Ossig, Sabrina Ritz, Lydia Sauer, Roland Buesen, Sascha Rehm, Wendel Wohlleben, Sibylle Groeters, Volker Strauss, Saskia Sperber, Haleluya Wami, Ulrich Dobrindt, Karola Prior, Dag Harmsen, Bennard van Ravenzwaay, Juergen Schnekenburger
Summary: The analysis of intestinal microbiome and plasma metabolome can serve as an early and sensitive indicator of gut microbiome changes induced by orally administered nanoparticles; this will help to recognize potential adverse effects of these changes to the host.
PARTICLE AND FIBRE TOXICOLOGY
(2022)
Article
Oncology
Rati Lama, Chao Xu, Samuel L. Galster, Javier Querol-Garcia, Scott Portwood, Cory K. Mavis, Federico M. Ruiz, Diana Martin, Jin Wu, Marianna C. Giorgi, Jill Bargonetti, Eunice S. Wang, Francisco J. Hernandez-Ilizaliturri, Gerald B. Koudelka, Sherry R. Chemler, Ines G. Munoz, Xinjiang Wang
Summary: This study identifies MMRi62 as a novel MDM2-MDM4-targeting agent that can induce apoptosis in leukemia cells, including those with non-functional p53.
FRONTIERS IN ONCOLOGY
(2022)
Article
Immunology
Paulo A. Schueroff, Cecilia M. Abe, Jonatas W. Silva, Cideli de Paula Coelho, Fernanda B. Andrade, Rodrigo T. Hernandes, Ulrich Dobrindt, Tania A. T. Gomes, Waldir P. Elias
Summary: This study evaluated the involvement of AFP and other adhesins in the uropathogenicity and intestinal colonization of bacteria. The results showed a synergistic role of AFP and TIF in the adherence and colonization of intestinal and urinary epithelia.
Article
Microbiology
Lang Peng, Rexford Mawunyo Dumevi, Marco Chitto, Nadja Haarmann, Petya Berger, Gerald Koudelka, Herbert Schmidt, Alexander Mellmann, Ulrich Dobrindt, Michael Berger
Summary: This study proposes a new method for genetic manipulation of enterohemorrhagic Escherichia coli (EHEC) that is more efficient than traditional methods. The method increases recombineering efficiency by 3-4 times in EHEC O157:H7 EDL933 Delta stx1/2, but shows similar efficiency in Escherichia coli K-12 MG1655. Additionally, the study suggests that the inhibition of the RecFOR pathway indirectly blocks phage induction and improves recombineering efficiency.
Article
Microbiology
Ying Liu, Gerald B. Koudelka
Summary: Protozoan predation is a major cause of bacterial mortality. The recognition and internalization of Escherichia coli by Acanthamoeba castellanii is mediated by the oligosaccharide (OS) region of lipopolysaccharide (LPS), with the Kdo2 moiety of the inner core being necessary and sufficient for recognition. The composition of the variable outer core OS region modulates the efficiency of bacterial consumption by A. castellanii.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Miguel Barquin, Ian U. Kouzel, Beat Ehrmann, Michael Basler, Andreas J. Gruber
Summary: This study presents a single cell-based Terminal Exon Annotation database (scTEA-db), which provides yet unannotated terminal exons and associated transcript isoforms for studying their biological role in cell identity and function.
NUCLEIC ACIDS RESEARCH
(2023)