Article
Biotechnology & Applied Microbiology
Wen-Fang Wang, Han Xiao, Jian-Jiang Zhong
Summary: In this study, a new GA, DHLDOA, was successfully synthesized in engineered yeast by oxidation of HLDOA using CYP5139G1 from Ganoderma lucidum. Enzymatic experiments confirmed the activity of CYP5139G1, and strategies to increase DHLDOA production were also explored.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Yifeng Zhang, Lin Ma, Ping Su, Luqi Huang, Wei Gao
Summary: Terpenoids, as the largest family of natural products, play significant roles in medicine, agriculture, cosmetics, and food. Traditional extraction methods have low yields, waste resources, and are not suitable for endangered species. Modern biotech approaches are promising in discovering and characterizing plant terpenoid-related P450 enzymes, and metabolic engineering strategies can be employed to increase terpenoid production.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Lihong Jiang, Lei Huang, Jin Cai, Zhinan Xu, Jiazhang Lian
Summary: This article discusses the strategies for improving the expression and activity of eukaryotic P450s in yeast, followed by examples of P450s involved in biosynthetic pathway engineering.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Lihong Jiang, Chang Dong, Tengfei Liu, Yi Shi, Handing Wang, Zeng Tao, Yan Liang, Jiazhang Lian
Summary: A novel strategy was reported to improve the functional expression of P450 enzymes in Saccharomyces cerevisiae by screening three new target genes from an Arabidopsis thaliana cDNA library, resulting in increased production efficiency of natural products.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jiazeng Yang, Yuguang Liu, Dacai Zhong, Linlin Xu, Haixin Gao, Jay D. Keasling, Xiaozhou Luo, Howard H. Chou
Summary: A combinatorial optimization approach was used to assemble cytochrome P450 monooxygenases and reductases, leading to the establishment of a CYP network capable of catalyzing multiple oxidation reactions. The target product titer was improved by remodeling endoplasmic reticulum size and controlling the configuration of CYPs on the endoplasmic reticulum.
METABOLIC ENGINEERING
(2023)
Review
Plant Sciences
Anqi Zhou, Kang Zhou, Yanran Li
Summary: This review highlights the potential of microbial bioproduction as an alternative sourcing to plant natural products, focusing on strategies to enhance the activity of plant CYPs in Escherichia coli and Saccharomyces cerevisiae. In-depth investigations on the native microenvironment of plant CYPs are essential for further improving the efficiency of functional reconstitution of plant CYPs in microbial systems.
CURRENT OPINION IN PLANT BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shotaro Uehara, Yuichi Iida, Miyuki Ida-Tanaka, Motohito Goto, Kenji Kawai, Masafumi Yamamoto, Yuichiro Higuchi, Satoshi Ito, Riichi Takahashi, Hidetaka Kamimura, Mamoru Ito, Hiroshi Yamazaki, Mitsuo Oshimura, Yasuhiro Kazuki, Hiroshi Suemizu
Summary: This study developed a novel humanized liver mouse model that can accurately evaluate human drug metabolism without interference from mouse hepatocytes.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Meghan E. Davies, Daniel Tsyplenkov, Vincent J. J. Martin
Summary: This study demonstrates for the first time de novo synthesis of nepetalactone in Saccharomyces cerevisiae, overcoming bottleneck in the pathway by introducing exogenous genes and optimizing gene copy numbers. By optimizing the pathway, the researchers were able to achieve high yields of nepetalactone from simple sugar in microtiter plates, paving the way for scalable production of the compound.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Junhua Wang, Wei Jiang, Chaojuan Liang, Linghuan Zhu, Youran Li, Qin Mo, Sha Xu, Alex Chu, Liang Zhang, Zhongyang Ding, Guiyang Shi
Summary: This study aimed to maximize the flux of farnesyl diphosphate to farnesene biosynthesis in Saccharomyces cerevisiae. Through screening and genetic engineering, a high alpha-farnesene producing yeast strain was developed, reaching a production level of 10.4 g/L. Various strategies were employed, such as overexpressing key enzymes and optimizing gene expression, to achieve the significant improvement in farnesene production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Review
Biotechnology & Applied Microbiology
Shuaiqi Meng, Yu Ji, LeiLei Zhu, Gaurao Dhoke, Mehdi D. Davari, Ulrich Schwaneberg
Summary: Cytochrome P450s are important enzymes widely used in pharmaceuticals and biosynthesis. Engineering them can improve coupling efficiency and reduce undesirable reactions.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Microbiology
Pan Zhu, Rui Luo, Yize Li, Xiulai Chen
Summary: This study successfully improved the production efficiency and acid tolerance of L-lactic acid by pathway and transporter engineering in Saccharomyces cerevisiae. The engineered strain achieved high levels of L-lactic acid production through adaptive evolution.
MICROBIOLOGY SPECTRUM
(2022)
Article
Plant Sciences
Pramesti Istiandari, Shuhei Yasumoto, Pisanee Srisawat, Keita Tamura, Ayaka Chikugo, Hideyuki Suzuki, Hikaru Seki, Ery Odette Fukushima, Toshiya Muranaka
Summary: Research reveals that different plant CPR classes have varied effects on triterpenoid production when co-expressed with different CYP classes. Co-expression of these genes in yeast can lead to higher yields and more valuable compounds.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Zhan-Tao Zhu, Meng-Meng Du, Bei Gao, Xin-Yi Tao, Ming Zhao, Yu-Hong Ren, Feng-Qing Wang, Dong-Zhi Wei
Summary: Harnessing mitochondria for terpene biosynthesis is promising but can lead to metabolic burden and poor cell growth. Analysis shows toxicity from MVA pathway phosphorylated intermediates within mitochondria inhibits cellular functions. Combining cytoplasmic and mitochondrial engineering strategies can alleviate metabolic burden and enhance terpene production.
METABOLIC ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Tianxiang Xiao, Kai Lu
Summary: The common cutworm, Spodoptera litura, has developed resistance to commonly used insecticides. Two P450 enzymes, CYP6AE43 and CYP6AE48, play a crucial role in detoxifying pyrethroids and promoting resistance development in S. litura.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Physical
Yuhuan Luo, Zeqian Du, Chenjian Jiang, Ziqian Yu, Jian-Jiang Zhong, Ting Shi, Han Xiao
Summary: A new type of CYP512W2 cytochrome P450 enzyme was discovered in this study, which can efficiently catalyze the production of important GAs. Further engineering can increase the yield and reveal the catalytic mechanism.
Article
Biotechnology & Applied Microbiology
Xiaoting Lan, Han Xiao
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2020)
Article
Biotechnology & Applied Microbiology
Yubo Fang, Mingyu Luo, Xin Song, Ying Shen, Han Xiao
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2020)
Review
Biotechnology & Applied Microbiology
Shuobo Shi, Zhihui Wang, Lirong Shen, Han Xiao
Summary: This article introduces the application of synthetic biology in food production, including microbial production of food components and expanding the utilization of feedstocks. Synthetic biology offers potential solutions to high-quality food and the greenhouse effect.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Yubo Fang, Han Xiao
Summary: Efficient secretion of triterpenoids can be achieved by overexpressing YPS3 and SCW10 genes in engineered Saccharomyces cerevisiae strains, resulting in increased production of GA-HLDOA and changes in cell permeability and outer mannan layer.
ACS SYNTHETIC BIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Zhao Chang, Ye Li, Yinhua Lu, Han Xiao
Summary: This review discusses the discovery and rational engineering of key enzymes, C3 oxidases and acetyltransferases, involved in the post-modification of terpenoids and steroids. Strategies for obtaining excellent biocatalysts are proposed based on the discovery strategy and reaction mechanism.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Ziqian Yu, Zhao Chang, Yinhua Lu, Han Xiao
Summary: Metabolic engineering of Saccharomyces cerevisiae for efficient glycerol utilization is vital for the manufacturing industry. Glycerol, a widely available alternative feedstock, is considered promising for microbial fermentation. This review introduces the metabolic pathway and regulation of glycerol in S. cerevisiae, and summarizes various strategies for improving glycerol utilization, including metabolic engineering, adaptive evolution, and reverse metabolic engineering. Proposed methods for further enhancing glycerol utilization provide insights for designing engineered S. cerevisiae.
FEMS YEAST RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Xin Song, Han Xiao, Shangwen Luo, Xiaozheng Wang, Wenfang Wang, Shuangjun Lin
BIORESOURCES AND BIOPROCESSING
(2019)
Review
Biotechnology & Applied Microbiology
Qin Wang, Shu Quan, Han Xiao
BIORESOURCES AND BIOPROCESSING
(2019)