4.7 Article

Electrochemical detection of DNA hybridization based on three-dimensional ZnO nanowires/graphite hybrid microfiber structure

期刊

BIOELECTROCHEMISTRY
卷 128, 期 -, 页码 126-132

出版社

ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2019.04.003

关键词

DNA hybridization; Electrochemical impedance spectroscopy; Zinc oxide; Graphite fibers; Hybrids electrodes

资金

  1. Major Program of Shandong Province Natural Science Foundation [ZR2018ZC0843]
  2. Natural Science Foundation of Shandong Province [ZR2018BEM010]
  3. National Natural Science Foundation of China [51802115]

向作者/读者索取更多资源

In the present work, zinc oxide (ZnO) nanowire (NWs)/graphite microfiber hybrid electrodes were used for the detection of DNA hybridization. ZnO NWs were in-situ synthesized onto graphite fibers using the hydrothermal technique, and the result was characterized by X-ray diffraction (XRD), scanning electron microscope (SEM) as well as transmission electron microscope (TEM). An electrochemical sensor for the analysis of DNA hybridization was developed based on immobilizing DNA probes onto the ZnO NWs/graphite microfiber electrodes surface via electrostatic interaction. Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and X-ray photo-electron spectroscopy (XPS) were used to corroborate the fabrication of the sensor. Based on the results, ZnO NWs/graphite microfiber hybrid electrodes showed a great hybridization capacity for the determination of target DNA. A wide dynamic detection range from 1.0 x 10(-14) M to 1.0 x 10(-7) M with an ultralow detection limit of 33 x 10(-15) M was achieved for detecting the target DNA. Moreover, it showed good selectivity and stability during the detection process. (C) 2019 Elsevier B.V. All rights reserved.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

Article Engineering, Environmental

Regulation of functional groups enable the metal-free PDINH/GO advisable antibacterial photocatalytic therapy

Longwei Wang, Xiaowen Tang, Zhongwei Yang, Jiawei Guo, Zhen You, Yu Cai, Xiaochen Niu, Xiaoyu Zhang, Liwei Zhang, Jian Zhang, Aizhu Wang, Jing Liu, Hong Liu, Xin Yu

Summary: Functional group-regulated PDINH/GO has enhanced photocatalytic properties, exhibiting effective antibacterial effect and accelerating wound healing. It establishes a successful example of activating nanomaterials by regulating functional groups.

CHEMICAL ENGINEERING JOURNAL (2023)

Letter Materials Science, Multidisciplinary

2D Co metal-organic framework nanosheet as an oxidase-like nanozyme for sensitive biomolecule monitoring

Jia-Wei Guo, Zhong-Wei Yang, Xiu-Li Liu, Li-Wei Zhang, Wei-Bo Guo, Jian Zhang, Long-Hua Ding

Summary: Nanozyme-based biomolecules sensitive and quantitative detection is achieved using two-dimensional (2D) cobalt-metal-organic framework (Co-MOF) nanosheets with oxidase-like activity. These nanosheets exhibit high stability, large specific surface area, and numerous active sites. They show excellent performance in the quantitative determination of dopamine and glutathione with a wider dynamic sensing range and lower detection limits. This study expands the application of 2D-MOF structures in bioassays and provides new insights into the use of OXD-like nanozymes.

RARE METALS (2023)

Article Chemistry, Multidisciplinary

A Molybdenum Disulfide Nanozyme with Charge-Enhanced Activity for Ultrasound-Mediated Cascade-Catalytic Tumor Ferroptosis

Longwei Wang, Xiaodi Zhang, Zhen You, Zhongwei Yang, Mengyu Guo, Jiawei Guo, He Liu, Xiaoyu Zhang, Zhuo Wang, Aizhu Wang, Yawei Lv, Jian Zhang, Xin Yu, Jing Liu, Chunying Chen

Summary: The deficient catalytic activity of nanozymes and insufficient endogenous H2O2 in the tumor microenvironment (TME) are major obstacles for nanozyme-mediated catalytic tumor therapy. We explored the contributing factors of enzymatic activity based on positive and negative charges, which are experimentally and theoretically demonstrated to enhance the peroxidase (POD)-like activity of a MoS2 nanozyme. Hence, an acidic tumor microenvironment-responsive and ultrasound-mediated cascade nanocatalyst (BTO/MoS2@CA) is presented that enables effective tumor ferroptosis with minimal side effects.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2023)

Article Chemistry, Physical

Construction of S-N-C bond for boosting bacteria-killing by synergistic effect of photocatalysis and nanozyme

Longwei Wang, Zhongwei Yang, Guoxin Song, Zhen You, Xiaoyu Zhang, Lin Liu, Jian Zhang, Longhua Ding, Na Ren, Aizhu Wang, Jing Liu, Hong Liu, Xin Yu

Summary: A porous sulfur doped graphitic carbon nitride (g-SCN) was synthesized for ecofriendly, metal-free and low systemic toxicity, with the ability to kill both gram-negative and gram-positive bacteria. Sulfur doping broadens the absorption spectrum and promotes photocarrier separation, while the sulfur element coordinates with nitrogen to endow g-SCN with nanozyme-like activity. The synergistic effect of photocatalysis and nanozyme enables g-SCN to exhibit an antibacterial efficiency up to 100%.

APPLIED CATALYSIS B-ENVIRONMENTAL (2023)

Article Physics, Applied

Epitaxial GaSb films directly grown on on-axis Si(001) with low defect density by MBE

Dong Han, Wen-Qi Wei, Ming Ming, Zihao Wang, Ting Wang, Jian-Jun Zhang

Summary: In recent years, there has been a strong demand for GaSb-on-Si direct heteroepitaxy to expand the operating wavelength range to mid-infrared and high-mobility applications. However, the growth of high-quality GaSb films on Si is challenging due to the generation of high-density defects. In this study, we proposed a novel design and growth strategy to achieve the annihilation of antiphase boundaries (APBs) and the reduction of threading dislocation density (TDD) through the use of a V-grooved Si hollow structure and InGaSb/GaSb dislocation filtering layers. The reported results greatly improve the overall quality of epitaxial Si-based antimonide, benefiting various devices and critical applications.

APPLIED PHYSICS LETTERS (2023)

Review Chemistry, Multidisciplinary

Application of bismuth sulfide based nanomaterials in cancer diagnosis and treatment

Zhongwei Yang, Longwei Wang, Jian Zhang, Jing Liu, Xin Yu

Summary: Cancer is the most threatening disease to human life and health. Nanomaterials based on Bi2S3 have attracted attention in the biomedical field due to their special properties. This review summarizes the synthesis, properties, recent research progress, safety, and biodistribution of Bi2S3-based nanomaterials. The practical applications of these materials are expected in the near future.

NANO TODAY (2023)

Article Immunology

Polymyxin B complexation enhances the antimicrobial potential of graphene oxide

Santosh Pandit, Lucas Jacquemin, Jian Zhang, Zhengfeng Gao, Yuta Nishina, Rikke Louise Meyer, Ivan Mijakovic, Alberto Bianco, Chengfang Pang

Summary: The absorption of antimicrobial peptide polymyxin B onto the surface of graphene oxide significantly enhances its antibacterial activity and can effectively combat bacterial biofilms.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2023)

Article Chemistry, Physical

Polydopamine/graphene oxide coatings loaded with tetracycline and green Ag nanoparticles for effective prevention of biofilms

Jian Zhang, Priyanka Singh, Zhejian Cao, Shadi Rahimi, Santosh Pandit, Ivan Mijakovic

Summary: The development of antibacterial coatings is important for preventing biofilm formation on medical devices. This study presents a simple method to fabricate antibacterial coatings by combining the properties of polydopamine and graphene oxide. The coatings successfully loaded with tetracycline and green-synthesized silver nanoparticles exhibited strong inhibitory effects on Staphylococcus aureus and Escherichia coli biofilms, with over 1000-fold reduction in biofilm formation. The results suggest that these coatings have potential as an approach to prevent infection associated with implantable biomedical devices.

APPLIED SURFACE SCIENCE (2023)

Review Chemistry, Multidisciplinary

Nano-Bio Interactions: Biofilm-Targeted Antibacterial Nanomaterials

Bo Li, Jiahui Mao, Jiawei Wu, Kerou Mao, Yangrui Jia, Fulin Chen, Jing Liu

Summary: This review introduces the biological characteristics of biofilm and discusses the interactions between biofilm and nanomaterials. The focus is on nanomaterials-based antibacterial strategies, including biofilm targeting, preventing biofilm formation, enhancing biofilm penetration, disrupting the mature biofilm, and acting as drug delivery systems. The current advances in nanomaterials for antibacterial nanomedicines via biofilm targeting are also summarized.
Review Chemistry, Multidisciplinary

Piezoelectric Effect Modulates Nanozyme Activity: Underlying Mechanism and Practical Application

Xin Yu, Longwei Wang, Zhiling Zhu, Xun Han, Jian Zhang, Aizhu Wang, Longhua Ding, Jing Liu

Summary: This article comprehensively reviews the potential mechanisms and practical applications of regulating nanozyme activity through the piezoelectric effect. It highlights how the piezoelectric effect enables nanozymes to exhibit catalytic activity, and how the generated charges and electric field enhance nanozyme activity. The article also explores the practical applications of piezoelectric nanozymes in water pollutant degradation, bacterial disinfection, biological detection, and tumor therapy.
Review Engineering, Biomedical

Recent Development of Copper-Based Nanozymes for Biomedical Applications

Xin Yu, Yawen Wang, Jian Zhang, Jing Liu, Aizhu Wang, Longhua Ding

Summary: This comprehensive review highlights the recent advancements in Cu-based nanozymes and their emerging applications in the field of biochemistry. It discusses the types of enzymes mimicked by Cu-based nanomaterials and the regulation of activity in Cu-based nanozymes, as well as their applications in constructing sensors, antibacterial strategies, and cancer therapy.

ADVANCED HEALTHCARE MATERIALS (2023)

Article Nanoscience & Nanotechnology

Antibiotic-Loaded Boron Nitride Nanoconjugate with Strong Performance against Planktonic Bacteria and Biofilms

Jian Zhang, Nisha Neupane, Puspa Raj Dahal, Shadi Rahimi, Zhejian Cao, Santosh Pandit, Ivan Mijakovic

Summary: Developed antibiotic-loaded boron nitride nanoconjugates with strong antimicrobial properties for combating bacterial biofilms.

ACS APPLIED BIO MATERIALS (2023)

Article Nanoscience & Nanotechnology

Biomimetic Antibacterial Gelatin Hydrogels with Multifunctional Properties for Biomedical Applications

Hengzhi Ruan, Marko Bek, Santosh Pandit, Alexandra Aulova, Jian Zhang, Philip Bjellheim, Martin Lovmar, Ivan Mijakovic, Roland Kadar

Summary: This study presents a facile approach of incorporating dopamine and [2-(methacryloyloxy) ethyl] dimethyl-(3-sulfopropyl) ammonium hydroxide via in situ synthesis into gelatin hydrogels. The resulting hydrogels show exceptional antibacterial efficiency and multifunctional performance, making them highly promising for various biomedical applications.

ACS APPLIED MATERIALS & INTERFACES (2023)

Review Chemistry, Multidisciplinary

Nanozyme-Enhanced Electrochemical Biosensors: Mechanisms and Applications

Zhongwei Yang, Jiawei Guo, Longwei Wang, Jian Zhang, Longhua Ding, Hong Liu, Xin Yu

Summary: This article provides an overview of the mechanisms and applications of nanozymes in electrochemical biosensors. It introduces the definition and characteristics of nanozymes, emphasizing their role in constructing efficient sensors. It discusses common categories of nanozyme materials and their applications in electrochemical biosensors. The article showcases the practical applications of nanozyme-based electrochemical biosensors in various domains and provides insights into the future development of this field.
Article Biochemistry & Molecular Biology

Clinical validation of electrochemical biosensor for the detection of methylglyoxal in subjects with type-2 diabetes mellitus

Priyanga Kumar, Noel Nesakumar, Jayashree Gopal, Sakthivel Sivasubramanian, Srinivasan Vedantham, John Bosco Balaguru Rayappan

Summary: This study developed an electrochemical biosensor for measuring MG levels in the plasma of type-2 diabetes mellitus patients. The biosensor showed a wide linear concentration range, high sensitivity, and a 90% correlation with ELISA data. It also exhibited a significant correlation with HbA1c and fasting plasma glucose levels.

BIOELECTROCHEMISTRY (2024)

Article Biochemistry & Molecular Biology

A disposable immunosensor for the detection of salivary MMP-8 as biomarker of periodontitis

Cristina Tortolini, Valeria Gigli, Antonio Angeloni, Federico Tasca, Nguyen T. K. Thanh, Riccarda Antiochia

Summary: The development of a novel voltammetric immunosensor for the detection of salivary MMP-8 at the point-of-care is described. The sensor showed good performance and comparable results to the conventional ELISA method when tested in real saliva samples. This biosensor is single-use, cost-effective, and requires a small quantity of test medium and a short preparation time.

BIOELECTROCHEMISTRY (2024)

Article Biochemistry & Molecular Biology

Electrocatalytic aptasensor for bacterial detection exploiting ferricyanide reduction by methylene blue on mixed PEG/aptamer monolayers

Rimsha B. Jamal, Ulrich Bay Gosewinkel, Elena E. Ferapontova

Summary: Pathogen-triggered infections are a severe global threat to human health. Researchers have developed a fast and inexpensive electrocatalytic aptamer assay for the specific and ultrasensitive detection of E. coli, allowing for timely treatment and prevention. The method is fast, sensitive, and can be used in field and point-of-care applications for analysis of bacteria in the human environment.

BIOELECTROCHEMISTRY (2024)

Article Biochemistry & Molecular Biology

A ratiometric molecular imprinted electrochemiluminescence sensor based on enhanced luminescence of CdSe@ZnS quantum dots by MXene@NaAsc for detecting uric acid

Miao Liu, Yuwei Wang, Shanshan Tang, Wei Wang, Axin Liang, Aiqin Luo

Summary: An unlabeled ratiometric molecular imprinted electrochemiluminescence sensor was developed for the determination of trace uric acid using MXene@NaAsc, CdSe@ZnS quantum dots, and molecularly imprinted polymer composites modified glass carbon electrode. This sensor, with easy preparation, great selectivity, and excellent sensitivity, successfully detected uric acid in human serum.

BIOELECTROCHEMISTRY (2024)

Article Biochemistry & Molecular Biology

Bacterial eradication by a low-energy pulsed electron beam generator

Charlotte Da Silva, Camille Lamarche, Carole Pichereaux, Emmanuelle Mouton-Barbosa, Gauthier Demol, Sebastien Boisne, Etienne Dague, Odile Burlet-Schiltz, Flavien Pillet, Marie-Pierre Rols

Summary: Low-energy electron beams (LEEB) are a safe and practical sterilization solution for industrial applications. To address the limitations of LEEB, we developed a low-energy pulsed electron beam generator (LEPEB) that can effectively and efficiently eradicate bacteria in a wide range of industrial applications.

BIOELECTROCHEMISTRY (2024)

Article Biochemistry & Molecular Biology

N-methyl mesoporphyrin IX (NMM) as electrochemical probe for detection of guanine quadruplexes

Daniel Dobrovodsky, Ales Danhel, Daniel Renciuk, Jean-Louis Mergny, Miroslav Fojta

Summary: In this study, N-methyl mesoporphyrin IX (NMM) was utilized as a voltammetric probe for the electrochemical detection of G4s. The detection of NMM was achieved by cyclic voltammetry on a hanging mercury drop electrode (HMDE) with a limit of detection (LOD) of 40 nM. The reduction signal of NMM was found to be significantly higher when G4 oligodeoxynucleotides (G4 ODNs) were present compared to single- or double-stranded ODNs or unfolded ODNs capable of forming G4s. Gradual transition from unstructured single strand to G4, induced by increasing concentrations of the G4 stabilizing K+ ions, was detected for the first time using electrochemical methods. Circular dichroism spectroscopy provided support for the obtained results. This work expands on the utilization of electrochemical probes for DNA secondary structure recognition and offers a proof of principle for the development of novel electroanalytical methods for nucleic acid structure studies.

BIOELECTROCHEMISTRY (2024)

Article Biochemistry & Molecular Biology

Facile construction of nanocubic Mn3[Fe(CN)6]2@Pt based electrochemical DNA sensors for ultrafast precise determination of SARS-CoV-2

Mengjiao Zhu, Yu Liu, Meiyue Wang, Tao Liu, Zhenyu Chu, Wanqin Jin

Summary: Early rapid diagnosis of COVID-19 is crucial for reducing the risk of severe symptoms and loss of lung function. Researchers have proposed an ultrafast and ultrasensitive DNA sensor that can accurately detect the virus in a short period of time, with outstanding selectivity.

BIOELECTROCHEMISTRY (2024)

Article Biochemistry & Molecular Biology

Development of bioanodes rich in exoelectrogenic bacteria using iron-rich palaeomarine sediment inoculum

Fatima-Zahra Ait-Itto, James A. Behan, Mathieu Martinez, Frederic Barriere

Summary: This study investigated the feasibility of using ancient marine sediments as inoculum for bioanode development in microbial fuel cells (MFC). The results showed the presence of two exoelectrogenic bacterial genera in these iron-rich sediments and confirmed that the development of the bioanode derived from the native microbiota. This study has important implications for understanding the role of these bacteria in broader paleoenvironmental phenomena.

BIOELECTROCHEMISTRY (2024)

Article Biochemistry & Molecular Biology

Paper-based electrodes as a tool for detecting ligninolytic enzymatic activities

Issa Fall, Bastien Doumeche, Sofiene Abdellaoui, Caroline Remond, Harivony Rakotoarivonina, Marjorie Ochs

Summary: This article presents a novel electrochemical tool based on lignin-coated paper electrodes for the detection and characterization of ligninolytic activity. The suitability of this method has been demonstrated using a catalaseperoxidase isolated from Thermobacillus xylanilyticus.

BIOELECTROCHEMISTRY (2024)

Article Biochemistry & Molecular Biology

Proximity hybridization induced bipedal DNA walker and rolling circle amplification for label-free electrochemical detection of apolipoprotein A4

Chenyi Zhuo, Dehong Yu, Jiuying Cui, Zichun Song, Qianli Tang, Xianjiu Liao, Zhao Liu, Ning Xin, Lu Lou, Fenglei Gao

Summary: A label-free detection strategy for quantification of Apolipoprotein A4 (Apo-A4) has been developed based on rolling circle amplification (RCA) and proximity hybridization-induced DNAzyme-driven bipedal DNA walker. This method shows high sensitivity and selectivity for a wide range of protein targets.

BIOELECTROCHEMISTRY (2024)

Article Biochemistry & Molecular Biology

Polyethylene glycol hydrogel coatings for protection of electroactive bacteria against chemical shocks

Niloufar Fattahi, Jeffrey Reed, Evan Heronemus, Priyasha Fernando, Ryan Hansen, Prathap Parameswaran

Summary: In this study, polyethylene glycol hydrogels were developed as protective coatings for electroactive biofilms, improving their viability under low resource conditions and ammonia-N shocks.

BIOELECTROCHEMISTRY (2024)

Article Biochemistry & Molecular Biology

Self-cleaning paper-based microfluidic biosensor employing DNAzyme and semiconducting single-walled carbon nanotube for copper ion detection

Xiaopeng Hou, Qiongyi Cheng, Hui Wang

Summary: A self-cleaning paper-based microfluidic biosensor was developed using hydrophobic paper decorated with polymeric octadecyl trichlorosilane. The biosensor showed improved performance and could effectively measure the concentration of copper ions in livestock feed and manure.

BIOELECTROCHEMISTRY (2024)

Article Biochemistry & Molecular Biology

Magnetically induced self-assembly electrochemical biosensor with ultra-low detection limit and extended measuring range for sensitive detection of HER2 protein

Yanling Zhang, Jie Wang, Min Liu, Yun Ni, Yao Yue, Dawei He, Ruijiang Liu

Summary: An innovative electrochemical biosensor has been developed for sensitive detection of HER2 protein, an important tumor marker for breast cancer diagnosis and treatment evaluation. The biosensor utilizes specific recognition probes and nanomaterials, along with a unique sensing strategy, to achieve ultra-low detection limit and demonstrate excellent selectivity, reproducibility, and stability.

BIOELECTROCHEMISTRY (2024)

Article Biochemistry & Molecular Biology

Nitrogen-enhanced carbon quantum dots mediated immunosensor for electrochemical detection of HER2 breast cancer biomarker

Humayun Amir, Vasanth Subramanian, Sundaram Sornambikai, Nagamony Ponpandian, Chinnusamy Viswanathan

Summary: In this study, the electrochemical detection of breast cancer biomarker HER2 was successfully achieved using the N-CQDs/GS as the detector. The BSA-modified HER2 Antibody/N-CQDs/GS immunoelectrode preserved excellent activity for the biosensor, while the GS electrode provided high stability and conductivity. The method exhibited a low detection limit and optimal specificity, stability, and reproducibility for detecting HER-2 protein in untreated blood samples from breast cancer patients.

BIOELECTROCHEMISTRY (2024)

Article Biochemistry & Molecular Biology

Polypyrrole-based structures for activation of cellular functions under electrical stimulation

Ilona Uzieliene, Anton Popov, Raminta Vaiciuleviciute, Gailute Kirdaite, Eiva Bernotiene, Almira Ramanaviciene

Summary: This review discusses the application of polypyrrole (Ppy), an electroconductive polymer, in cell culture experiments with electrical stimulation (ES) and its potential for tissue regeneration. Ppy can direct ES to cells and stimulate differentiation towards different cell lineages. Different types of Ppy materials and their combination with active molecules are explored.

BIOELECTROCHEMISTRY (2024)