Article
Cell Biology
Laura Mainz, Mohamed A. F. E. Sarhan, Sabine Roth, Ursula Sauer, Charis Kalogirou, Markus Eckstein, Elena Gerhard-Hartmann, Helen-Desiree Seibert, Hans-Ulrich Voelker, Carol Geppert, Andreas Rosenwald, Martin Eilers, Almut Schulze, Markus Diefenbacher, Mathias T. Rosenfeldt
Summary: The potential therapeutic target of macroautophagy/autophagy for various diseases, including cancer, is mainly based on preclinical mouse studies. This study introduces a shRNA transgenic mouse model that allows simultaneous knockdown of Atg7 in most organs, providing a valuable tool for further understanding of the role of autophagy impairment at different disease stages.
Article
Oncology
Ziva Frangez, Deborah Gerard, Zhaoyue He, Marios Gavriil, Yuniel Fernandez-Marrero, S. Morteza Seyed Jafari, Robert E. Hunger, Philippe Lucarelli, Shida Yousefi, Thomas Sauter, Lasse Sinkkonen, Hans-Uwe Simon
Summary: Research found significantly reduced expression of ATG5 and ATG7 in melanoma tissues, correlated with decreased autophagic activity and unfavorable clinical outcomes. NRF1 was identified to promote the production of ATG5 and ATG7, showing involvement in their regulation.
FRONTIERS IN ONCOLOGY
(2021)
Review
Cell Biology
Kaja Urbanska, Arkadiusz Orzechowski
Summary: While ATG5 and ATG7 were thought to be crucial for autophagy, recent research has revealed the existence of an alternative autophagy pathway independent of ATG5/ATG7. This alternative pathway differs in its molecular mechanism from the canonical pathway and may play a role in mitophagy. Further studies are needed to fully understand the function and significance of alternative autophagy in various cellular processes and disease pathogenesis.
Article
Multidisciplinary Sciences
Jaclyn S. Long, Elzbieta Kania, David G. McEwan, Valentin J. A. Barthet, Martina Brucoli, Marcus J. G. W. Ladds, Christoph Nossing, Kevin M. Ryan
Summary: Research findings show that the loss of Atg7 in a model of PDAC with mutant Kras(G12D) and mutant Trp53(172H/+) promotes tumor development and enhances metastasis, while reducing the occurrence of metastasis. Tumors with Atg7(+/-) have lower levels of succinate and lower invasion capabilities.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Nemanja Vujic, Ivan Bradic, Madeleine Goeritzer, Katharina B. Kuentzel, Silvia Rainer, Dagmar Kratky, Branislav Radovic
Summary: The study shows that loss of ATG5 results in depletion of LC3-II, while loss of ATG7 has no significant impact on LC3-PE conjugation. The level of LC3 lipidation in macrophages under inflammatory stimuli is determined by their metabolic status, with ATG7 playing a supportive but nonessential role.
Article
Medicine, Research & Experimental
Kaiju Sun, Yangyang Du, Yuzhu Hou, Mingyue Zhao, Jiajia Li, Yazhe Du, Lingxiao Zhang, Changbao Chen, Hongmei Yang, Fei Yan, Rui Su
Summary: The study revealed that SsD has broad anti-proliferation effects in AML by targeting FTO/m(6)A signaling pathway, increasing global m(6)A RNA methylation levels, and suppressing relevant pathways affecting AML cell proliferation.
Article
Biochemistry & Molecular Biology
Andrea Barrientos-Riosalido, Monica Real, Laia Bertran, Carmen Aguilar, Salome Martinez, David Parada, Margarita Vives, Fatima Sabench, David Riesco, Daniel Del Castillo, Cristobal Richart, Teresa Auguet
Summary: This study analyzed the expression of ATG7 gene in obesity-associated NAFLD and found that ATG7 was highly expressed in NASH patients. ATG7 may play an important role in the pathogenesis of NAFLD, especially in NASH, possibly having a protective effect.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Cell Biology
Jack J. Collier, Monika Olahova, Thomas G. McWilliams, Robert W. Taylor
Summary: ATG7 plays a crucial role in autophagy, and dysfunction of ATG7 may lead to neurological issues. Studies have identified patients with specific ATG7 variants suffering from neurodevelopmental disorders, with the pathology being linked to attenuated autophagy.
Review
Biochemistry & Molecular Biology
Harish Changotra, Sargeet Kaur, Suresh Singh Yadav, Girdhari Lal Gupta, Jyoti Parkash, Ajay Duseja
Summary: Autophagy is a crucial process in maintaining cellular homeostasis, and ATG5 plays a central role in this process. In addition to its role in autophagy, ATG5 also has autophagy-independent functions and is associated with various diseases.
CELL BIOCHEMISTRY AND FUNCTION
(2022)
Article
Critical Care Medicine
Jian Jin, Kai-si Zhu, Shu-min Tang, Yang Xiang, Ming-yi Mao, Xu-dong Hong, Ai-fen Chen, Xu-dong Zhang, Hao Lu, Zheng-li Chen, Jin-fang Wu, Sun-feng Pan, Shi-hui Zhu
Summary: Trehalose reduces stress-induced wound tissue damage by promoting cell proliferation and migration, reducing necrosis and apoptosis, thus promoting wound healing.
Article
Cardiac & Cardiovascular Systems
Yong Wang, Xiaoguang Lu, Xiaoping Wang, Qi Qiu, Ping Zhu, Lin Ma, Xiao Ma, Joerg Herrmann, Xueying Lin, Wei Wang, Xiaolei Xu
Summary: The study aimed to elucidate the dynamic autophagic defects in AIC and identify a mechanism-based therapy through genetic and pharmacological studies. Results showed that atg7-based autophagy activation is an effective therapeutic avenue to reverse the decline in cardiac function in AIC, highlighting the time-dependent nature of autophagy-based therapy.
CIRCULATION RESEARCH
(2021)
Article
Cell Biology
Bora Lee, Hyejin Shin, Ji-Eun Oh, Jaekyoung Park, Mira Park, Seung Chel Yang, Jin-Hyun Jun, Seok-Ho Hong, Haengseok Song, Hyunjung Jade Lim
Summary: The study revealed that the deficit of autophagy in the uterine vessel microenvironment provokes hyperpermeability through the deregulation of VEGFA, NOS1, and CTNNB1, affecting the vascular stability and function.
Review
Medicine, Research & Experimental
Jack J. Collier, Fumi Suomi, Monika Olahova, Thomas G. McWilliams, Robert W. Taylor
Summary: ATG proteins are essential for macroautophagy and play important roles beyond autophagic elimination. Dysfunction of ATG7 is associated with diseases, but the mechanisms of how it contributes to these diseases remain unclear. Further research on the relationship between ATG7 dysfunction and diseases will help develop therapies for disorders involving ATG7 deficiency and impaired autophagy.
EMBO MOLECULAR MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Hu-Qiang He, Yuan-Qing Qu, Betty Yuen Kwan Law, Cong-Ling Qiu, Yu Han, Ivo Ricardo de Seabra Rodrigues Dias, Yong Liu, Jie Zhang, An-Guo Wu, Cheng-Wen Wu, Simon Wing Fai Mok, Xin Cheng, Yan-Zheng He, Vincent Kam Wai Wong
Summary: The study found that calcification was upregulated in HASMCs treated with AGEs when autophagy and apoptosis were activated. However, AGEs-activated calcification and apoptosis were suppressed in Atg7 knockout cells or pretreated with wortmannin (WM), an autophagy inhibitor. This suggests potential clinical applications for autophagy inhibitors in the treatment of diabetes-related vascular calcification.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Cell Biology
Han Wang, Zongwen Liang, Yanling Gou, Zhi Li, Yingying Cao, Na Jiao, Jiahuan Tan, Yangyang Yu, Zongfeng Zhang
Summary: The m6A methylation mechanism of FTO plays an important role in regulating the development of endometriosis. FTO downregulation in endometriotic stromal cells leads to decreased ATG5 expression, which in turn affects glycolysis, proliferation, and metastasis through the ATG5/PKM2 pathway.
CELLULAR SIGNALLING
(2022)
Review
Food Science & Technology
Liyi Wang, Ziye Xu, Defeng Ling, Jie Li, Yizhen Wang, Tizhong Shan
Summary: This article summarizes and discusses the roles of various dietary factors in regulating skeletal muscle development and function, and reviews their potential regulatory molecular mechanisms.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Biotechnology & Applied Microbiology
Cheng Wang, Siyu Wei, Nana Chen, Yun Xiang, Yizhen Wang, Mingliang Jin
Summary: This study investigated the gut microbiota characteristics in 120 pigs of different breeds, growth periods, and genders, revealing that gut microbiota plays important roles in nutrition, metabolism, and immunity. The top 10 most abundant genera accounted for 66.53% of the microbiota, and 28 common species were identified. The explanatory effects of influencing factors suggested that growth period was the greatest contributor to the gut microbiota in pigs.
MICROBIAL BIOTECHNOLOGY
(2022)
Review
Nutrition & Dietetics
Bocheng Xu, Jinping Cao, Jie Fu, Zhi Li, Mingliang Jin, Xinxia Wang, Yizhen Wang
Summary: This meta-analysis examined the effects of nondigestible fermentable carbohydrates (NDFCs) on adiposity and cardiovascular risk factors in adults with overweight or obesity. The findings suggest that increased intake of NDFCs is significantly effective in reducing body weight, waist and hip circumference, blood pressure, cholesterol levels, and low-density lipoprotein in individuals with overweight and obesity.
Article
Biology
Wei Chen, Yushi Chen, Ruifan Wu, Guanqun Guo, Youhua Liu, Botao Zeng, Xing Liao, Yizhen Wang, Xinxia Wang
Summary: This study found that DHA can promote mitochondrial biogenesis and skeletal muscle fiber remodeling through the FTO/m(6)A/DDIT4/PGC1 alpha signaling pathway, protecting against obesity-induced decline in skeletal muscle function.
Article
Biochemistry & Molecular Biology
Ruifan Wu, Shuting Cao, Fan Li, Shengchun Feng, Gang Shu, Lina Wang, Ping Gao, Xiaotong Zhu, Canjun Zhu, Songbo Wang, Qingyan Jiang
Summary: Promoting the thermogenic function of brown adipose tissue (BAT) is a promising strategy to combat obesity and metabolic disorders. Y-box binding protein 1 (YBX1), an RNA binding protein, plays a critical role in the post-transcriptional regulation of thermogenesis through enhancing mitophagy by targeting key regulators of mitophagy. Loss of YBX1 inhibits brown adipocyte differentiation and thermogenic function.
Article
Genetics & Heredity
Botao Zeng, Ruifan Wu, Yushi Chen, Wei Chen, Youhua Liu, Xing Liao, Guanqun Guo, Xinxia Wang
Summary: This study found that knockout of the FTO gene in adipose tissue can alleviate hepatic steatosis caused by obesity and improve the expression levels of genes related to hepatic lipid metabolism. Further experiments demonstrated that FTO knockout increased the levels of IL-6 in adipose tissues and 3T3-L1 cells, and the increase in IL-6 inhibited the expression of lipolysis-related genes. Finally, both depletion of FTO in adipocytes and IL-6 supplementation led to increased expression of lipolysis genes in AML12 cells.
Article
Biochemistry & Molecular Biology
Youhua Liu, Ying Yang, Ruifan Wu, Chun-chun Gao, Xing Liao, Xiao Han, Botao Zeng, Chaoqun Huang, Yaojun Luo, Yuxi Liu, Yushi Chen, Wei Chen, Jiaqi Liu, Qin Jiang, Yuanling Zhao, Zhen Bi, Guanqun Guo, Yongxi Yao, Yun Xiang, Xiaojun Zhang, Teresa G. Valencak, Yizhen Wang, Xinxia Wang
Summary: This study reveals the regulatory role of mRNA m(5)C modification in adipogenesis and myogenesis. Higher levels of mRNA m(5)C are associated with lower fat and higher muscle mass. m(5)C modification inhibits lipid accumulation and promotes myogenic differentiation. YBX2 and SMO are identified as m(5)C targets, which are involved in the regulation of adipogenesis and myogenesis through ALYREF-mediated subcellular localization.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Veterinary Sciences
Zipeng Jiang, Weifa Su, Chaoyue Wen, Wentao Li, Yu Zhang, Tao Gong, Shuai Du, Xinxia Wang, Zeqing Lu, Mingliang Jin, Yizhen Wang
Summary: Clostridium perfringens is a main pathogen that can cause various diseases in humans and animals. This study aimed to build a mouse model infected with porcine C. perfringens and investigated its effects on growth performance, immune status, and intestinal integrity in mice. The results showed that high dose of C. perfringens caused a decline in growth performance, severe damage to intestinal structure, and increased inflammation in mice.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Environmental Sciences
Weike Shaoyong, Yalin Liu, Bocheng Xu, Bo Pan, Xinuer Xianmi, Yizhen Wang, Mingliang Jin
Summary: This study found that exposure to BDE-47 can cause ovarian lipid deposition and hormone disruption accompanied by oxidative stress and downregulation of hormone biosynthesis-related proteins. Using a cellular model, it was shown that BDE-47 inhibits ovarian hormone secretion by interfering with specific pathways and inducing endoplasmic reticulum stress, mitochondrial abnormalities, and lipotoxicity. The findings suggest potential health risks of BDE-47 pollution areas to women, particularly affecting their ovarian hormone secretion.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Engineering, Civil
Hai-Ling Shi, Lun-Yang Zhao, Qi-Zhi Zhu
Summary: In this article, a series of conventional triaxial compression tests were performed to investigate the strength characteristics and deformation behavior of red sandstone. The experimental results show that the red sandstone exhibits increased initial yield stress, volume dilatation stress, and peak strength as the confining pressure increases. Moreover, the failure modes of the specimens change from splitting failure to compressive-shear failure with increasing confining pressure. The non-linear Hoek-Brown criterion is found to be suitable for fitting the strength data. An elastoplastic damage model with a unified plastic hardening/softening law is developed based on the experiments. As a contribution, analytical solutions to the constitutive equations are derived for the conventional triaxial loading path under consideration. The model is validated by comparing the analytical predictions with the experimental data.
EUROPEAN JOURNAL OF ENVIRONMENTAL AND CIVIL ENGINEERING
(2023)
Article
Cell Biology
Ruifan Wu, Shengchun Feng, Fan Li, Gang Shu, Lina Wang, Ping Gao, Xiaotong Zhu, Canjun Zhu, Songbo Wang, Qingyan Jiang
Summary: Obesity is a global health problem associated with metabolic diseases. This study investigates the role of YBX1 in adipogenesis and adipose tissue expansion. The researchers found that YBX1 deficiency inhibited adipocyte differentiation and that YBX1 promotes adipogenesis through ULK1- and ULK2-mediated autophagy. The findings provide insights into the molecular mechanisms and functional importance of YBX1 in regulating autophagy and adipogenesis, highlighting its potential as a therapeutic target for obesity and metabolic diseases.
CELL DEATH & DISEASE
(2023)
Article
Multidisciplinary Sciences
Ruifan Wu, Jooman Park, Yanyu Qian, Zuoxiao Shi, Ruoci Hu, Yexian Yuan, Shaolei Xiong, Zilai Wang, Gege Yan, Sang-Ging Ong, Qing Song, Zhenyuan Song, Abeer M. Mahmoud, Pingwen Xu, Congcong He, Robert W. Arpke, Michael Kyba, Gang Shu, Qingyan Jiang, Yuwei Jiang
Summary: Inhibiting Cdkn2a-Becn1 mediated autophagy can maintain beige adipocytes and provide long term metabolic health benefits in mice.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Hailing Shi, Yichun He, Yiming Zhou, Jiahao Huang, Kamal Maher, Brandon Wang, Zefang Tang, Shuchen Luo, Peng Tan, Morgan Wu, Zuwan Lin, Jingyi Ren, Yaman Thapa, Xin Tang, Ken Y. Chan, Benjamin E. Deverman, Hao Shen, Albert Liu, Jia Liu, Xiao Wang
Summary: Spatially charting molecular cell types in the central nervous system of adult mice, this study creates a high-resolution molecular atlas and identifies previously unknown tissue architectures.
Article
Multidisciplinary Sciences
Hu Zeng, Jiahao Huang, Jingyi Ren, Connie Kangni Wang, Zefang Tang, Haowen Zhou, Yiming Zhou, Hailing Shi, Abhishek Aditham, Xin Sui, Hongyu Chen, Jennifer A. Lo, Xiao Wang
Summary: The precise control of mRNA translation is crucial in posttranscriptional gene regulation, but it is challenging to study it systematically at the transcriptomic scale with spatial and single-cell resolution. The researchers developed a method called RIBOmap to detect cellular translatome. The study revealed cell cycle-dependent translational control and colocalized translation of functional gene modules.
Article
Cell Biology
Ziye Xu, Wentao Chen, Liyi Wang, Wenjing You, Yanfang Wang, Yizhen Wang, Jianguo Zhao, Tizhong Shan
Summary: This study investigated the lipid profiles and regulatory pathways in the skeletal muscles of UCP1 KI pigs using lipidomics and transcriptomics. The results showed altered contents of glycerophospholipids and acyl carnitines in UCP1 KI skeletal muscles, as well as enriched metabolic regulatory pathways. Comparison of transcriptomes suggested a possible crosstalk between adipose and skeletal muscle tissues through nervous system or chemokine signaling in UCP1 KI pigs. Additionally, associations between UCP1 KI-induced metabolic alterations and metabolic and muscle dysfunction were identified using lipid biomarkers.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)