Article
Agronomy
Marlene Lachaux, Emilie Thomas, Adam J. Bogdanove, Boris Szurek, Mathilde Hutin
Summary: We identified and characterized TalD and TalI as two African Xoo TALEs with avirulence activity on IR64 and CT13432 respectively. Resistance of CT13432 against African Xoo results from the combination of two mechanisms, one relying on the TalI-mediated induction of an unknown executor gene and the other on an Xa1-like gene or allele.
Article
Plant Sciences
Alvaro L. Perez-Quintero, R. Luis M. Rodriguez, Sara Cuesta-Morrondo, Eliska Hakalova, Daniela Betancurt-Anzola, Laura Carolina Camelo Valera, Luis Alberto Chica Cardenas, Luisa Matiz-Ceron, Jonathan M. Jacobs, Veronica Roman-Reyna, Alejandro Reyes Munoz, Adriana Jimena Bernal Giraldo, Ralf Koebnik
Summary: Strains of Xanthomonas citri pv. malvacearum cause bacterial blight of cotton, posing a serious threat to cotton production worldwide. The activity of TAL effectors is associated with the development of disease symptoms, and the pathogen exhibits genetic diversity and variation in TAL effector genes. Pseudogenization of TAL effector genes is observed, which may be related to resistance genes.
Article
Biology
Fudan Chen, Bingxiao Yan, Xiangyu Gong, Helin Li, Zuhua He
Summary: The genome sequencing of Xoo strain DY89031 revealed a rich diversity of evolutionary features potentially associated with rice cultivation regions, and the effector proteins secreted by T3SS showed pathogenesis divergence. DY89031 exhibited a remarkable difference from the avirulent strain PXO99A, and plant inoculation experiments with PXO99A transformants expressing DY89031 genes identified additional effectors supporting DY89031-specific virulence. This characterization of DY89031 genome and discovery of new effectors will enhance understanding of rice-Xoo interaction and new mechanisms involved.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Plant Sciences
Ayaka Yoshihisa, Satomi Yoshimura, Motoki Shimizu, Koji Yamaguchi, Tsutomu Kawasaki
Summary: This study determined the whole genome sequence of Xanthomonas oryzae pv. oryzae (Xoo) strain T7133 and identified TAL and iTAL effectors, showing that the iTAL effector of Xoo T7133 may contribute to overcoming rice Xa1-mediated immunity.
JOURNAL OF GENERAL PLANT PATHOLOGY
(2021)
Article
Genetics & Heredity
Sruthi Sivaraman, Dineshkumar Krishnamoorthy, Kumar Arvind, Tony Grace, Jyotsana Sharma, Ginny Antony
Summary: This study investigates the molecular mechanism of pomegranate susceptibility to bacterial blight in India. Through sequencing and RNA-Seq analysis, the researchers identified TALEs in Xcp119 genome and potential host targets in the susceptible pomegranate variety Bhagwa. They discovered that Xcp119 encodes seven TALEs and predicted several candidate susceptibility genes.
Article
Biochemistry & Molecular Biology
Hinda Doucoure, Florence Auguy, Servane Blanvillain-Baufume, Sandrine Fabre, Marc Gabriel, Emilie Thomas, Fleur Dambreville, Coline Sciallano, Boris Szurek, Ousmane Koita, Valerie Verdier, Sebastien Cunnac
Summary: This study investigates the transcriptional activation of Xoo strains in rice. Genome editing can confer broad resistance on Asian Xoo strains but not on African Xoo strains. The study also identifies a possible off-target gene for TalC and documents the first example of divergent transcription activation by a native TALE during infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Doron Teper, Nian Wang
Summary: By using an experimental evolution approach, we identified adaptive mutations and deletions in TALEs that cannot recognize the EBE of host targets, which reconstituted virulence on the host in less than 30 cycles of repeated infections. Adaptive variants originated from TALEs with a small number of mismatches to the EBE, while TALEs with more mismatches failed to adapt during the study period. This study experimentally demonstrates adaptive rearrangements of TALEs during host adaptation and highlights the importance of considering resistance durability in modified crops introduced into the field.
Article
Plant Sciences
Naama Wagner, Daniella Ben-Meir, Doron Teper, Tal Pupko
Summary: Xanthomonas hortorum pv. pelargonii and Xanthomonas fragariae are causative agents of bacterial diseases in geranium and strawberry plants, respectively. They use the type III secretion system and effector proteins to infect plant cells. Through genome sequencing and validation, new effector proteins were identified in X. hortorum and X. fragariae.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Coline Sciallano, Florence Auguy, Gabriel Boulard, Boris Szurek, Sebastien Cunnac
Summary: In this study, we report the genome sequence of the CIX2779 strain of the rice bacterial blight pathogen Xanthomonas oryzae pv. oryzae, which originated from Niger. The genome consists of a circular chromosome and a circular plasmid. Importantly, we identified the plasmid as a genuine conjugative plasmid, the first one sequenced from an African strain of the X. oryzae species. This study is significant for understanding the characteristics of X. oryzae species in Africa.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2023)
Article
Plant Sciences
Shuo Duan, Yunfei Long, Shuyuan Cheng, Jinyun Li, Zhigang Ouyang, Nian Wang
Summary: A rapid evaluation method was developed to test the resistance of citrus plants against citrus bacterial canker (CBC), and citrus germplasms collected in China were evaluated. Two citrus germplasms were found to be resistant to CBC, while eight were tolerant.
Article
Plant Sciences
Syed Mashab Ali Shah, Moein Khojasteh, Qi Wang, Fazal Haq, Xiameng Xu, Ying Li, Lifang Zou, Ebrahim Osdaghi, Gongyou Chen
Summary: This study investigates the transcriptomic profile of wheat cultivars infected with Xanthomonas translucens pv. cerealis and reveals the differential gene expression patterns between different wheat cultivars. The results demonstrate the important roles of Type II and III secretion systems in the pathogenicity of X. translucens pv. cerealis and provide novel insights into host-pathogen interactions.
Article
Zoology
David R. Angelini, Joshua L. Steele, Michael C. Yorsz, Devin M. O'Brien
Summary: Polyphenism allows organisms to adopt alternative morphological traits and reproductive strategies in response to environmental changes. The soapberry bug Jadera haematoloma exhibits wing polyphenism in response to nutritional resources and cohort density, with differences in wing and thorax development correlated to female fecundity. Transcriptome sequencing reveals differential gene expression in the thorax and gonads of polyphenic morphs, and potential mediators of male fertility differences. Nutrient limitation also leads to gene expression related to cuticle structure and spermatogenesis. These findings provide insights into the nutrition-dependent polyphenism in J. haematoloma.
INTEGRATIVE AND COMPARATIVE BIOLOGY
(2022)
Article
Microbiology
Tianjiao Li, Yiming Li, Xiuguo Ma, Xue Dan, Xianjiao Huang, Qinying Li, Shimin Lei, Zhengchun Zhang, Sheng Huang, Wei Jiang, Yanhua Yu, Yong-Qiang He
Summary: This study reveals the genomic characteristics of two Xoo strains, GXO20-01 and GXO20-06, which caused the epidemic of rice bacterial leaf blight in Guangxi in 2020. Despite their high genomic similarity, these two strains differ in a few genes, including the TALE gene. The study also highlights the genomic differences in Xoo strains mainly focus on tal genes, and demonstrates the utility of HiFi long-read sequencing in TALE analysis and pathogen tracking.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Carlos Andres Zarate-Chaves, Corinne Audran, Cesar Augusto Medina Culma, Aline Escalon, Stephanie Javegny, Lionel Gagnevin, Emilie Thomas, Lea-Lou Pimpare, Camilo E. Lopez, Jonathan M. Jacobs, Laurent D. Noel, Ralf Koebnik, Adriana Jimena Bernal, Boris Szurek
Summary: A CRISPR interference strategy was successfully applied to investigate the cryptic role of the transcription activator-like effector (tale) multigene family in various plant-pathogenic Xanthomonas bacterial species. The results revealed their contribution to pathogen virulence and TALE-induced host gene expression. This technology provides a platform for discovery and rapid functional understanding of highly conserved gene families.
Article
Microbiology
In-Jeong Kang, Kyung Seok Kim, Gwyn A. Beattie, Jung-Wook Yang, Kee Hoon Sohn, Sunggi Heu, Ingyu Hwang
Summary: Analysis of pathogenicity genes of Xanthomonas citri pv. glycines strains from Korea and the United States revealed differences in candidate type III secretion system effector (T3SE) genes and diverse repeat sizes of transcription activator-like effectors (TALEs). Phylogenetic analysis showed two distinct clusters of TALEs with 17.5 repeats, suggesting adaptation to different soybean cultivars. The evolving target specificity of TALEs' binding domains in soybean cultivars enhances understanding of Xcg pathogenicity.
Article
Multidisciplinary Sciences
Ian Lorenzo Quibod, Alvaro Perez-Quintero, Nicholas J. Booher, Gerbert S. Dossa, Genelou Grande, Boris Szurek, Casiana Vera Cruz, Adam J. Bogdanove, Ricardo Oliva
SCIENTIFIC REPORTS
(2016)
Article
Biochemistry & Molecular Biology
Li Wang, Fabio C. Rinaldi, Pallavi Singh, Erin L. Doyle, Zoe E. Dubrow, Tuan Tu Tran, Alvaro L. Perez-Quintero, Boris Szurek, Adam J. Bogdanove
Editorial Material
Plant Sciences
Niklas Schandry, Jonathan M. Jacobs, Boris Szurek, Alvaro L. Perez-Quintero
MOLECULAR PLANT PATHOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Alvaro L. Perez-Quintero, Leo Lamy, Carlos A. Zarate, Sebastien Cunnac, Erin Doyle, Adam Bogdanove, Boris Szurek, Alexis Dereeper
MOLECULAR PLANT-MICROBE INTERACTIONS
(2018)
Article
Microbiology
Tuan T. Tran, Alvaro L. Perez-Quintero, Issa Wonni, Sara C. D. Carpenter, Yanhua Yu, Li Wang, Jan E. Leach, Valerie Verdier, Sebastien Cunnac, Adam J. Bogdanove, Ralf Koebnik, Mathilde Hutin, Boris Szurek
Article
Plant Sciences
Jillian M. Lang, Alvaro L. Perez-Quintero, Ralf Koebnik, Elysa DuCharme, Soungalo Sarra, Hinda Doucoure, Ibrahim Keita, Janet Ziegle, Jonathan M. Jacobs, Ricardo Oliva, Ousmane Koita, Boris Szurek, Valerie Verdier, Jan E. Leach
FRONTIERS IN PLANT SCIENCE
(2019)
Article
Microbiology
Dousheng Wu, Edda von Roepenack-Lahaye, Matthias Buntru, Orlando de Lange, Niklas Schandry, Alvaro L. Perez-Quintero, Zasha Weinberg, Tiffany M. Lowe-Power, Boris Szurek, Anthony J. Michael, Caitilyn Allen, Stefan Schillberg, Thomas Lahaye
CELL HOST & MICROBE
(2019)
Article
Biotechnology & Applied Microbiology
Ricardo Oliva, Chonghui Ji, Genelou Atienza-Grande, Jose C. Huguet-Tapia, Alvaro Perez-Quintero, Ting Li, Joon-Seob Eom, Chenhao Li, Hanna Nguyen, Bo Liu, Florence Auguy, Coline Sciallano, Van T. Luu, Gerbert S. Dossa, Sebastien Cunnac, Sarah M. Schmidt, Inez H. Slamet-Loedin, Casiana Vera Cruz, Boris Szurek, Wolf B. Frommer, Frank F. White, Bing Yang
NATURE BIOTECHNOLOGY
(2019)
Article
Plant Sciences
Alvaro L. Perez-Quintero, Mary Ortiz-Castro, Jillian M. Lang, Adrien Rieux, Guangxi Wu, Sanzhen Liu, Toni A. Chapman, Christine Chang, Janet Ziegle, Zhao Peng, Frank F. White, Maria Cristina Plazas, Jan E. Leach, Kirk Broders
Article
Biotechnology & Applied Microbiology
Alejandra Huerta, Emily E. Delorean, Ana M. Bossa-Castro, Bradley W. Tonnessen, Chitra Raghavan, Rene Corral, Alvaro L. Perez-Quintero, Hei Leung, Valerie Verdier, Jan E. Leach
Summary: The study reveals the significant contribution of the minor-effect virulence factor Tal7b to the disease resistance in rice bacterial blight caused by Xanthomonas oryzae pv.oryzae (Xoo). Through genome-wide association studies and interval mapping analysis, 18 QTL were identified, and among them, 6 were specific to Tal7b. The findings suggest that targeting strategic virulence factors like Tal7b could provide a potential new approach for effective disease resistance.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Microbiology
Carlos A. Zarate-Chaves, Daniela Osorio-Rodriguez, Ruben E. Mora, Alvaro L. Perez-Quintero, Alexis Dereeper, Silvia Restrepo, Camilo E. Lopez, Boris Szurek, Adriana Bernal
Summary: This study characterized the TALE diversity in Colombian strains of Xpm, identifying 13 novel variants and a functional variant that activates a susceptibility gene in cassava. Through transcriptomics and prediction analyses, several TALE-targeted candidate genes were selected, along with two potential cases of functional convergence. This research provides new insights into potential TALE targets in the Xpm-cassava interaction, which could be crucial for defining the infection outcome.
Article
Multidisciplinary Sciences
Emile Gluck-Thaler, Aude Cerutti, Alvaro L. Perez-Quintero, Jules Butchacas, Veronica Roman-Reyna, Vishnu Narayanan Madhavan, Deepak Shantharaj, Marcus Merfa, Celine Pesce, Alain Jauneau, Taca Vancheva, Jillian M. Lang, Caitilyn Allen, Valerie Verdier, Lionel Gagnevin, Boris Szurek, Gregg T. Beckham, Leonardo De La Fuente, Hitendra Kumar Patel, Ramesh Sonti, Claude Bragard, Jan E. Leach, Laurent D. Noel, Jason C. Slot, Ralf Koebnik, Jonathan M. Jacobs
Meeting Abstract
Plant Sciences
A. Perez-Quintero, M. C. Ortiz-Castro, G. Wu, J. M. Lang, S. Liu, T. Chapman, C. Chang, J. Ziegle, Z. Peng, F. F. White, M. C. Plazas, J. E. Leach, K. Broders
Article
Plant Sciences
Emily Luna, Leon van Eck, Tony Campillo, Margaret Weinroth, Jessica Metcalf, Alvaro L. Perez-Quintero, Anna-Maria Botha, Theodore W. Thannhauser, Darryl Pappin, Ned A. Tisserat, Nora L. V. Lapitan, Cristiana T. Argueso, Paul J. Ode, Michelle L. Heck, Jan E. Leach
PHYTOBIOMES JOURNAL
(2018)